##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933286.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	311194
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.499074532285327	31.0	28.0	33.0	25.0	34.0
2	28.815600557851372	31.0	28.0	33.0	16.0	34.0
3	28.843714853114136	31.0	28.0	34.0	16.0	34.0
4	33.13555209933354	35.0	32.0	37.0	28.0	37.0
5	34.20943848531784	35.0	33.0	37.0	30.0	37.0
6	33.71316927704262	35.0	33.0	37.0	28.0	37.0
7	33.46866906174284	35.0	32.0	37.0	28.0	37.0
8	33.55513602447348	35.0	33.0	37.0	28.0	37.0
9	34.610779770818205	37.0	34.0	39.0	28.0	39.0
10	34.32112122984376	37.0	32.0	39.0	27.0	39.0
11	34.56086235595802	37.0	33.0	39.0	27.0	39.0
12	34.68489752373118	37.0	33.0	39.0	27.0	39.0
13	34.86045039428781	37.0	34.0	39.0	27.0	39.0
14	35.64670591335309	38.0	34.0	40.0	27.0	41.0
15	35.997631702410715	38.0	34.0	40.0	27.0	41.0
16	35.84098986484315	38.0	34.0	40.0	27.0	41.0
17	35.24196803280269	37.0	33.0	40.0	27.0	41.0
18	34.98100220441268	37.0	33.0	39.0	27.0	40.0
19	34.32363413176346	37.0	32.0	38.0	27.0	40.0
20	33.90176867163249	35.0	32.0	38.0	27.0	40.0
21	34.22215081267634	35.0	33.0	38.0	27.0	40.0
22	34.800288565974924	35.0	33.0	39.0	29.0	40.0
23	35.18021555685521	35.0	34.0	39.0	30.0	40.0
24	34.645317711781075	35.0	33.0	39.0	27.0	40.0
25	33.62438864502529	35.0	33.0	38.0	24.0	40.0
26	33.66185402032173	35.0	32.0	38.0	24.0	40.0
27	34.33489720238822	35.0	33.0	39.0	26.0	40.0
28	34.17066845761808	35.0	33.0	39.0	25.0	40.0
29	34.28458774912113	35.0	33.0	39.0	25.0	40.0
30	33.49862786557581	35.0	33.0	39.0	22.0	40.0
31	33.392755001703115	35.0	33.0	39.0	22.0	40.0
32	32.90288694512105	35.0	32.0	39.0	18.0	40.0
33	32.4436685797284	35.0	31.0	39.0	15.0	40.0
34	32.03873467997455	35.0	31.0	39.0	12.0	40.0
35	31.563063555209933	35.0	30.0	40.0	10.0	41.0
36	31.05530312281085	35.0	28.0	40.0	8.0	41.0
37	30.931772463479373	35.0	27.0	40.0	8.0	41.0
38	30.60562221636664	35.0	25.0	39.0	8.0	40.0
39	30.23401800805928	35.0	24.0	39.0	7.0	40.0
40	29.973929445940474	35.0	23.0	39.0	7.0	40.0
41	29.501558513338946	35.0	22.0	39.0	7.0	40.0
42	29.690967692179154	35.0	23.0	39.0	7.0	40.0
43	29.619716318438016	35.0	22.0	39.0	7.0	40.0
44	29.732440214142947	35.0	23.0	39.0	7.0	40.0
45	29.83025379666703	35.0	23.0	39.0	7.0	40.0
46	29.562938231456904	35.0	23.0	39.0	7.0	40.0
47	29.49529232568751	35.0	23.0	39.0	7.0	40.0
48	29.346815812644202	35.0	23.0	38.0	7.0	40.0
49	29.52551463074481	35.0	23.0	39.0	7.0	40.0
50	29.51623424616156	35.0	23.0	39.0	7.0	40.0
51	28.28702031530171	33.0	20.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	12.0
10	30.0
11	24.0
12	31.0
13	43.0
14	53.0
15	99.0
16	182.0
17	332.0
18	659.0
19	1152.0
20	1750.0
21	2658.0
22	3794.0
23	5465.0
24	7867.0
25	11379.0
26	14454.0
27	15492.0
28	14777.0
29	13814.0
30	14377.0
31	16228.0
32	18578.0
33	22059.0
34	24544.0
35	28455.0
36	31887.0
37	32779.0
38	24749.0
39	3459.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.044525280050383	2.715347982287576	34.20502965995488	37.03509707770715
2	36.77480928295533	14.381061331516676	34.47977788774848	14.36435149777952
3	13.505080432142009	14.54141146680206	57.32308463530787	14.630423465748056
4	12.125233777000842	3.2079667345771448	67.3364525022976	17.33034698612441
5	20.43034248732302	3.676484765130433	57.96544920531886	17.927723542227678
6	14.97233237144675	12.901277016909066	60.74474443594671	11.381646175697476
7	55.70480150645578	1.4132663226154747	40.06150504187099	2.8204271290577583
8	55.28962640667879	9.23314716864721	32.27311580557466	3.2041106190993403
9	52.01835510967435	2.8724846880081234	31.775998251894315	13.333161950423209
10	32.44310622955455	16.393953610930804	38.19643052243938	12.966509637075266
11	25.722218294697196	16.134308502091944	43.55289626406679	14.59057693914407
12	19.535723696472296	14.99546906431358	47.644234786017726	17.8245724531964
13	18.651387880229052	15.19598706915943	52.976599805908855	13.176025244702661
14	16.26413105651137	19.876668573301544	47.3601676124861	16.499032757700984
15	13.596020488826904	15.356979890357783	54.77419230447887	16.272807316336433
16	16.766711440451935	16.94762752495228	45.49605712192395	20.78960391267184
17	17.01703760355277	17.391723490812804	48.61340514277268	16.977833762861753
18	17.094802599021833	16.154553108350417	47.84153936129874	18.90910493132901
19	16.98522465086088	18.303373458357168	45.388728574458376	19.32267331632358
20	18.66584831327082	18.951522201584865	48.865016677699444	13.517612807444873
21	18.361857876437206	21.455105175549658	46.182767019929685	14.000269928083448
22	16.879502818177727	14.47521481776641	48.716556231803956	19.928726132251906
23	16.611502792470294	20.815954035103506	47.798158062173435	14.77438511025277
24	17.98203050187343	17.586135979485466	45.4198988412373	19.011934677403804
25	16.335147849894277	23.621599388163013	43.43849817155858	16.604754590384132
26	16.397809726408607	16.19504232086737	46.59601406196778	20.81113389075625
27	17.961143209701984	17.98974273282904	45.281400026992806	18.767714030476164
28	13.906759127746678	18.654922652750376	48.0854386652699	19.352879554233052
29	18.279272736620886	16.70758433645893	44.47675726395753	20.53638566296265
30	16.282768947987428	19.654299247414798	48.96559702307885	15.097334781518922
31	19.447997069352237	16.345109481545276	44.87747193069275	19.329421518409738
32	22.932961432418363	19.36219849997108	42.08628701067501	15.618553056935545
33	18.12567080342166	17.948289491442637	42.35589375116487	21.570145953970833
34	19.562073818903965	19.700251290191968	39.74980237408176	20.987872516822303
35	20.06208345919266	21.953829444012417	39.64279516957268	18.341291927222247
36	19.695431145844715	23.64827085355116	36.985610262408656	19.670687738195465
37	21.634414545267582	22.121570467296927	38.529663168313014	17.714351819122477
38	20.752328129719725	22.629292338541234	38.303116383991984	18.315263147747064
39	22.886045360771735	21.96250570383748	33.65103440297692	21.500414532413863
40	23.36613173775844	21.14308116480395	37.813389718310766	17.677397379126848
41	19.329742861366224	25.75853004877986	33.53695765342519	21.37476943642872
42	21.62477425657307	22.169771910769487	37.64789809572164	18.5575557369358
43	20.96248642326009	22.83559451660379	34.10766274414031	22.094256315995807
44	22.075618424519753	21.784160362989002	34.69507766859258	21.445143543898663
45	19.6167021215062	21.413330591206773	34.786339068233964	24.183628219053066
46	23.235666497426045	23.24659215794649	32.84542761107219	20.672313733555274
47	17.49166115027925	21.938404982101197	41.10554830748665	19.464385560132907
48	19.934831648425096	24.179450760618778	33.136564329646454	22.749153261309665
49	19.915551071036074	21.615455310835042	37.588128305815665	20.88086531231322
50	19.44735438343927	21.884098022455444	35.97723606496269	22.691311529142595
51	19.411685315269576	21.215704672969274	34.22366755143094	25.148942460330215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	44.0
1	444.5
2	845.0
3	9772.0
4	18699.0
5	13893.0
6	9087.0
7	8652.5
8	8218.0
9	7984.0
10	7750.0
11	7315.0
12	6880.0
13	6385.0
14	5890.0
15	5562.5
16	5235.0
17	4808.0
18	4381.0
19	4044.0
20	3707.0
21	3485.5
22	3264.0
23	3229.0
24	3194.0
25	3217.5
26	3330.5
27	3420.0
28	3761.0
29	4102.0
30	4359.5
31	4617.0
32	5141.5
33	5666.0
34	6113.0
35	6560.0
36	6680.0
37	6800.0
38	7314.5
39	7829.0
40	8205.0
41	8581.0
42	9182.5
43	9784.0
44	10708.0
45	11632.0
46	25327.0
47	39022.0
48	28822.0
49	18622.0
50	17674.0
51	16726.0
52	15290.0
53	13854.0
54	13775.5
55	13697.0
56	13383.0
57	13069.0
58	12443.0
59	11817.0
60	10591.0
61	9365.0
62	8289.5
63	7214.0
64	6441.5
65	5669.0
66	4878.0
67	4087.0
68	3521.5
69	2956.0
70	2484.5
71	2013.0
72	1728.0
73	1443.0
74	1222.0
75	804.0
76	607.0
77	443.5
78	280.0
79	225.5
80	171.0
81	135.5
82	100.0
83	62.5
84	25.0
85	22.0
86	19.0
87	13.5
88	8.0
89	5.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	311194.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.135142175892454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.94993930430273	40.371163711036004
2	7.766869908875937	7.943199941040638
3	4.274778161992961	6.557741672517308
4	2.8167641014074873	5.761425312056874
5	1.9492216691467463	4.983686359207464
6	1.3016715598502377	3.9936696167554566
7	0.8715351253537599	3.119625078237413
8	0.5931360305421652	2.426407620113449
9	0.4004106867327749	1.842755165534446
>10	1.006546902799883	8.080243847033612
>50	0.04566157364828471	1.6107643385283712
>100	0.016488901595213924	1.4956402515185925
>500	6.341885228928431E-4	0.18776576444693518
>1k	0.003805131137357059	2.4831130541799356
>5k	0.0025367540915713725	9.142798267793513
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9207	2.958604600345765	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	6708	2.1555685520929067	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	6684	2.147856321137297	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	5594	1.7975924985700238	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	1669	0.5363213943713567	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC	1436	0.4614484855106461	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGTCGAT	1250	0.401678695604671	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	1129	0.3627961978701389	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1096	0.3521918803061756	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTA	1077	0.3460863641329846	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTT	579	0.18605757180408364	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGT	420	0.13496404172316948	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05173621599388163	0.0
2	0.0	0.0	0.0	0.4800863769867028	0.0
3	0.0	0.0	0.0	0.5800240364531449	0.0
4	0.0	0.0	0.0	1.2484173859393175	0.0
5	0.0	0.0	0.0	3.825266553982403	0.0
6	0.0	0.0	0.0	4.108048355688092	0.0
7	0.0	0.0	0.0	4.610628739628656	0.0
8	0.0	0.0	0.0	5.212504097122695	0.0
9	0.0	0.0	0.0	5.318868615718812	0.0
10	0.0	0.0	0.0	7.945525942016877	0.0
11	0.0	0.0	0.0	8.113909651214355	0.0
12	0.0	0.0	0.0	10.34756454173281	0.0
13	0.0	0.0	0.0	10.52815928327667	0.0
14	0.0	0.0	0.0	10.674048985520287	0.0
15	0.0	0.0	0.0	11.143531044943026	0.0
16	0.0	0.0	0.0	11.380039460915055	0.0
17	0.0	0.0	0.0	11.490902780901946	0.0
18	0.0	0.0	0.0	11.58409223828223	0.0
19	0.0	0.0	0.0	12.169579104995597	0.0
20	0.0	0.0	0.0	12.303257774892833	0.0
21	0.0	0.0	0.0	12.410264979401916	0.0
22	0.0	0.0	0.0	12.618816558159862	0.0
23	0.0	0.0	0.0	12.720039589452238	0.0
24	0.0	0.0	0.0	12.817085162310327	0.0
25	0.0	0.0	0.0	12.918629536559187	0.0
26	0.0	0.0	0.0	13.026922112894207	0.0
27	0.0	0.0	0.0	13.187272248179593	0.0
28	0.0	0.0	0.0	13.284639163994164	0.0
29	0.0	0.0	0.0	13.395823826937537	0.0
30	0.0	0.0	0.0	13.541070843268187	0.0
31	0.0	0.0	0.0	13.661253108993105	0.0
32	0.0	0.0	0.0	13.795253121846823	0.0
33	0.0	0.0	0.0	13.918648817136576	0.0
34	0.0	0.0	0.0	14.087996555203507	0.0
35	0.0	0.0	0.0	14.259915036922306	0.0
36	0.0	0.0	0.0	14.409660854643727	0.0
37	0.0	0.0	0.0	14.572903076537465	0.0
38	0.0	0.0	0.0	14.756068561733196	0.0
39	0.0	0.0	0.0	15.095406723780021	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACCG	20	7.025349E-4	45.0	27
CGCGCCA	20	7.025349E-4	45.0	38
CCTACAC	70	0.0	45.0	28
TACGGTG	25	3.884227E-5	45.0	7
CCAGATA	20	7.025349E-4	45.0	13
CTACGAA	20	7.025349E-4	45.0	11
CGAATAT	20	7.025349E-4	45.0	14
GCTACGA	20	7.025349E-4	45.0	10
ATACGGT	25	3.884227E-5	45.0	6
AGCTACG	20	7.025349E-4	45.0	9
ACGAGGG	20	7.025349E-4	45.0	3
CGTTTTT	7455	0.0	44.245476	1
GATACCT	885	0.0	42.203392	5
GCCCTAC	75	0.0	42.0	26
TACGGCT	795	0.0	41.88679	7
GTATGAT	65	0.0	41.538464	1
TGATACC	900	0.0	41.25	4
ATACCTG	915	0.0	41.065575	6
TCGTCGG	50	1.0768417E-9	40.5	2
TACCTGT	930	0.0	40.40323	7
>>END_MODULE