##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933285.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	225477
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.736514145566954	31.0	28.0	31.0	16.0	33.0
2	29.195283776172293	31.0	30.0	33.0	16.0	34.0
3	29.41576302682757	31.0	30.0	33.0	16.0	34.0
4	32.21188413895874	35.0	32.0	37.0	19.0	37.0
5	33.60659845571832	35.0	33.0	37.0	28.0	37.0
6	33.00531318050178	35.0	33.0	37.0	25.0	37.0
7	31.869068685497854	35.0	30.0	35.0	25.0	37.0
8	33.09444865773449	35.0	33.0	35.0	28.0	37.0
9	32.78239909170337	35.0	31.0	37.0	25.0	39.0
10	30.90181260172878	35.0	27.0	39.0	15.0	39.0
11	31.713895430576066	35.0	27.0	39.0	15.0	39.0
12	32.727249342505	35.0	30.0	39.0	21.0	39.0
13	32.85787463909845	35.0	31.0	39.0	21.0	39.0
14	33.24283186311686	36.0	31.0	40.0	18.0	41.0
15	33.84778048315349	36.0	31.0	40.0	23.0	41.0
16	34.016312085046366	36.0	32.0	40.0	23.0	41.0
17	32.02655259738244	35.0	27.0	39.0	17.0	41.0
18	32.73890906833069	36.0	30.0	39.0	21.0	39.0
19	32.52857275908408	36.0	30.0	37.0	18.0	39.0
20	32.35637781237111	34.0	30.0	37.0	24.0	39.0
21	32.67270275903972	35.0	31.0	37.0	23.0	39.0
22	32.971504854153636	35.0	31.0	37.0	25.0	39.0
23	34.04628409993037	35.0	33.0	38.0	27.0	40.0
24	32.22018653787304	35.0	31.0	37.0	21.0	39.0
25	31.421745898694766	34.0	29.0	37.0	18.0	39.0
26	30.74068752023488	33.0	25.0	37.0	19.0	39.0
27	32.341768783512286	34.0	30.0	37.0	22.0	39.0
28	32.43037649072854	35.0	31.0	38.0	21.0	39.0
29	32.73356040749167	35.0	31.0	38.0	22.0	40.0
30	31.64222071430789	35.0	30.0	38.0	18.0	40.0
31	31.86366236911082	35.0	30.0	37.0	20.0	40.0
32	31.726765922910097	35.0	30.0	37.0	18.0	40.0
33	31.77865591612448	35.0	30.0	38.0	16.0	40.0
34	31.028291133907228	35.0	30.0	38.0	12.0	40.0
35	30.428070268807904	35.0	27.0	38.0	10.0	40.0
36	29.80537261006666	34.0	25.0	38.0	8.0	40.0
37	29.885810969633265	35.0	25.0	38.0	8.0	40.0
38	29.771781600784117	35.0	24.0	38.0	8.0	40.0
39	29.286219880519965	34.0	23.0	38.0	7.0	40.0
40	29.02928014830781	34.0	23.0	38.0	8.0	40.0
41	28.514549155789727	33.0	21.0	37.0	7.0	40.0
42	28.96336211675692	34.0	23.0	38.0	7.0	40.0
43	28.996868860238518	34.0	22.0	38.0	7.0	40.0
44	28.951334282432356	34.0	22.0	38.0	7.0	40.0
45	29.194316936982485	34.0	23.0	38.0	7.0	40.0
46	28.93570519387787	34.0	23.0	38.0	7.0	40.0
47	28.980250757283446	34.0	23.0	38.0	7.0	40.0
48	28.7670050603831	34.0	23.0	37.0	7.0	40.0
49	28.562004106849034	34.0	23.0	37.0	7.0	39.0
50	28.732686704187124	34.0	23.0	37.0	7.0	40.0
51	27.077870470158818	32.0	20.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	7.0
11	18.0
12	30.0
13	33.0
14	71.0
15	136.0
16	251.0
17	407.0
18	753.0
19	1204.0
20	1842.0
21	2622.0
22	3777.0
23	5155.0
24	6948.0
25	9366.0
26	11832.0
27	13036.0
28	12822.0
29	12975.0
30	14420.0
31	16101.0
32	18081.0
33	20196.0
34	22435.0
35	23694.0
36	16897.0
37	8698.0
38	1633.0
39	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.873969407079215	2.7510566487934467	37.09602309769954	39.2789508464278
2	40.01605485260137	8.93173139610692	36.91862141149652	14.13359233979519
3	14.111417129019813	8.828838418109164	62.48752644393885	14.572218008932175
4	12.600398266785525	3.2579819671185977	66.422295843922	17.719323922173878
5	17.140550920936505	3.6575792652909165	61.65994757780173	17.54192223597085
6	15.173609725160436	8.54676973704635	63.948429329820776	12.331191207972433
7	56.260283753997086	1.4449367341236579	39.294473493970564	3.0003060179087004
8	57.925642083228	5.841394022450183	33.01755833189194	3.215405562429871
9	53.90483286543638	2.6579207635368576	33.37413572116003	10.063110649866728
10	31.107829179916358	16.640721670059474	39.412002111080064	12.839447038944105
11	23.746546210921736	16.73474456374708	45.46805217383591	14.050657051495275
12	19.449877371084412	15.182923313686098	49.342948504725534	16.024250810503954
13	17.825764933895698	15.306660989812709	53.2262714157098	13.64130266058179
14	15.715128372295178	16.63761714055092	50.56568962687991	17.081564860273996
15	14.056422606296874	15.157643573402165	54.728420193633944	16.05751362666702
16	16.587944668414075	15.435720716525411	48.29494804348115	19.681386571579363
17	17.095756995170238	15.743512642087662	49.94034868301423	17.22038167972787
18	16.971575814828118	14.811266781090755	49.81927203218067	18.39788537190046
19	17.08289537292052	17.45499540973137	47.30903817240783	18.153071044940283
20	18.23733684588672	18.030663881460192	49.7452068281909	13.986792444462184
21	18.307410511936915	18.83784155368397	47.88648066099868	14.968267273380434
22	17.21417262071076	14.843642588822807	49.28041441033897	18.661770380127464
23	16.615441929775542	19.421936605507437	48.33131538915277	15.631306075564247
24	16.86912634104587	16.945409066113175	47.702426411563046	18.483038181277912
25	16.408768965349015	19.48314018724748	45.813985461931814	18.29410538547169
26	15.9976405575735	16.883761980157622	48.00134825281514	19.117249209453735
27	16.01050217982322	17.25275748745992	48.120207382571174	18.616532950145693
28	14.312324538644742	17.557888387729125	49.39084696000036	18.73894011362578
29	17.497571814420095	16.27128265854167	46.921415488054215	19.30973003898402
30	16.253985994136873	18.04042097420136	49.4582596007575	16.24733343090426
31	18.23911086274875	16.86557830732181	46.716516540489714	18.178794289439722
32	20.359948021305943	18.462636987364565	44.89770575269318	16.279709238636315
33	17.763230839509127	17.856810228981228	44.32203728096435	20.05792165054529
34	18.411190498365688	19.74569468282796	42.17015482732163	19.67295999148472
35	18.14065292690607	21.800449713274524	41.672543097522144	18.386354262297264
36	19.40109190737858	21.74501168633608	39.99121861653295	18.86267778975239
37	20.677940543824867	21.88870705216053	40.13402697392638	17.299325430088214
38	20.061913188484855	21.66828545705327	40.86891345902243	17.400887895439446
39	20.944486577344918	21.217685174097582	37.3514815258319	20.4863467227256
40	22.077639847966754	20.873525902863708	39.44393441459661	17.604899834572926
41	18.781960022530015	23.688890662905752	37.22463932019674	20.304509994367496
42	20.19230342784408	21.75033373692217	39.34503297453843	18.712329860695327
43	19.727067505776645	21.77029142662001	37.524448169879854	20.978192897723495
44	20.426917157847583	21.53346017553897	37.4330863014853	20.60653636512815
45	18.86489531082993	21.220789703606133	37.574564146232206	22.339750839331728
46	20.455744931855577	22.408050488519894	36.29727200557041	20.838932574054116
47	17.492693268049514	21.536564705047525	41.47695773848331	19.49378428841966
48	19.246752440381947	22.773054457882623	36.58599324986584	21.39419985186959
49	19.175791765900733	21.366259086292615	38.62699964963167	20.83094949817498
50	18.777968484590446	21.51527650270316	37.94843819990509	21.758316812801308
51	18.879530949941678	20.842480607778178	36.99313011970179	23.284858322578355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	247.0
2	428.0
3	10191.0
4	19954.0
5	13345.5
6	6737.0
7	6550.5
8	6364.0
9	6229.5
10	6095.0
11	5651.5
12	5208.0
13	4869.0
14	4530.0
15	4097.5
16	3665.0
17	3365.5
18	3066.0
19	2796.0
20	2526.0
21	2364.0
22	2202.0
23	2038.0
24	1874.0
25	1924.5
26	2056.0
27	2137.0
28	2420.0
29	2703.0
30	2889.0
31	3075.0
32	3474.5
33	3874.0
34	4156.5
35	4439.0
36	4630.5
37	4822.0
38	5236.5
39	5651.0
40	5937.0
41	6223.0
42	6726.5
43	7230.0
44	7888.5
45	8547.0
46	13727.0
47	18907.0
48	16769.0
49	14631.0
50	14032.0
51	13433.0
52	12051.0
53	10669.0
54	10262.0
55	9855.0
56	9527.0
57	9199.0
58	8607.0
59	8015.0
60	7415.0
61	6815.0
62	6190.5
63	5566.0
64	4960.5
65	4355.0
66	3847.0
67	3339.0
68	2874.0
69	2409.0
70	2064.5
71	1720.0
72	1461.5
73	1203.0
74	1011.5
75	709.0
76	598.0
77	430.0
78	262.0
79	212.5
80	163.0
81	120.5
82	78.0
83	53.5
84	29.0
85	21.0
86	13.0
87	8.0
88	3.0
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	225477.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.34575402076329
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.23413771263102	36.63139499510958
2	10.725594652347917	10.585251108823488
3	5.37686291804584	7.959760648717607
4	3.205129809201369	6.326381886778669
5	2.190125029257908	5.403668548423886
6	1.3538279050864948	4.008339527450606
7	0.9349812560642665	3.229614855304011
8	0.5281571709242571	2.0849851072584498
9	0.3501144007059199	1.554899318672515
>10	1.0294548959921366	7.809829006791313
>50	0.04276962137421462	1.4405384182898762
>100	0.021882131865877247	1.9053390595575124
>500	0.0029839270726196247	0.8221130666560621
>1k	0.0029839270726196247	3.3772895592002166
>5k	0.0	0.0
>10k+	9.946423575398749E-4	6.860594892966231
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13978	6.199301924364791	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCG	2924	1.2968063261441298	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGC	2105	0.9335763736434315	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTC	1852	0.8213698071200167	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCT	628	0.2785206473387529	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTGTCGAT	537	0.23816176372756423	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	510	0.2261871499088599	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTC	412	0.18272373678911819	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTA	315	0.13970382788488406	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCT	260	0.11531109603196779	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCTTGTCGATCGTATGCCG	254	0.11265007073892237	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09091836417905153	0.0
2	0.0	0.0	0.0	0.4647924178519317	0.0
3	0.0	0.0	0.0	0.6080442794608762	0.0
4	0.0	0.0	0.0	1.122065665234148	0.0
5	0.0	0.0	0.0	2.2889252562345606	0.0
6	0.0	0.0	0.0	2.599821711305366	0.0
7	0.0	0.0	0.0	2.923579788625891	0.0
8	0.0	0.0	0.0	3.3484568270821415	0.0
9	0.0	0.0	0.0	3.516101420544002	0.0
10	0.0	0.0	0.0	5.420952026149009	0.0
11	0.0	0.0	0.0	5.934529907706772	0.0
12	0.0	0.0	0.0	7.398980827312764	0.0
13	0.0	0.0	0.0	7.612306354971904	0.0
14	0.0	0.0	0.0	7.723182408848796	0.0
15	0.0	0.0	0.0	8.042505444014246	0.0
16	0.0	0.0	0.0	8.275788661371227	0.0
17	0.0	0.0	0.0	8.479800600504708	0.0
18	0.0	0.0	0.0	8.672281430034992	0.0
19	0.0	0.0	0.0	9.114011628680531	0.0
20	0.0	0.0	0.0	9.308266475072845	0.0
21	0.0	0.0	0.0	9.557072339972592	0.0
22	0.0	0.0	0.0	9.859098710733246	0.0
23	0.0	0.0	0.0	10.108791584064006	0.0
24	0.0	0.0	0.0	10.291071816637617	0.0
25	0.0	0.0	0.0	10.473352049211227	0.0
26	0.0	0.0	0.0	10.615716902389158	0.0
27	0.0	0.0	0.0	10.790901067514646	0.0
28	0.0	0.0	0.0	10.942136005002727	0.0
29	0.0	0.0	0.0	11.123529229145323	0.0
30	0.0	0.0	0.0	11.344394328468091	0.0
31	0.0	0.0	0.0	11.52179601467112	0.0
32	0.0	0.0	0.0	11.718268382140971	0.0
33	0.0	0.0	0.0	11.882364941878773	0.0
34	0.0	0.0	0.0	12.096577477968928	0.0
35	0.0	0.0	0.0	12.355583939825348	0.0
36	0.0	0.0	0.0	12.540081693476496	0.0
37	0.0	0.0	0.0	12.717926883895032	0.0
38	0.0	0.0	0.0	12.950323092820998	0.0
39	0.0	0.0	0.0	13.380522181863338	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCCG	25	3.8808008E-5	45.000004	40
ATTCCAA	25	3.8808008E-5	45.000004	21
GGGCATA	25	3.8808008E-5	45.000004	7
CACCGGT	35	1.2061173E-7	45.000004	16
CCGTTGG	25	3.8808008E-5	45.000004	2
GTTTGCG	20	7.021214E-4	45.0	1
GATACTG	20	7.021214E-4	45.0	5
TGATACT	20	7.021214E-4	45.0	4
ATCAGGA	20	7.021214E-4	45.0	16
TAGCGGG	20	7.021214E-4	45.0	3
GGCATAT	20	7.021214E-4	45.0	8
CGTTTTT	6095	0.0	44.40935	1
TACGGCT	355	0.0	41.197186	7
ATACGGC	690	0.0	41.086956	6
GGACTAA	55	6.002665E-11	40.909092	8
CGACCAC	220	0.0	40.909092	12
CGGGATT	55	6.002665E-11	40.909092	6
GAATGAT	1215	0.0	40.74074	1
ACGGCTG	360	0.0	40.625	8
ACGGCGA	360	0.0	40.625	8
>>END_MODULE