##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933284.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	577585
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.59164278850732	31.0	28.0	33.0	25.0	34.0
2	29.075644277465653	31.0	28.0	33.0	16.0	34.0
3	29.08272375494516	31.0	28.0	34.0	16.0	34.0
4	33.2449249893955	35.0	32.0	37.0	28.0	37.0
5	34.23904706666551	35.0	33.0	37.0	30.0	37.0
6	33.83848437892258	35.0	33.0	37.0	28.0	37.0
7	33.50446947202576	35.0	32.0	37.0	28.0	37.0
8	33.66476968757845	35.0	33.0	37.0	28.0	37.0
9	34.71939195096826	37.0	34.0	39.0	28.0	39.0
10	34.4589419739086	37.0	32.0	39.0	27.0	39.0
11	34.70835807716613	37.0	34.0	39.0	27.0	39.0
12	34.79679008284495	37.0	34.0	39.0	27.0	39.0
13	34.98864755836803	37.0	34.0	39.0	27.0	39.0
14	35.76706978193686	38.0	34.0	40.0	27.0	41.0
15	36.160594544525914	38.0	34.0	40.0	27.0	41.0
16	36.03045266064735	38.0	34.0	40.0	28.0	41.0
17	35.36857778508791	37.0	33.0	40.0	27.0	41.0
18	35.14785183133218	37.0	33.0	39.0	27.0	40.0
19	34.43200394747093	37.0	33.0	38.0	27.0	40.0
20	33.984241280504165	35.0	32.0	38.0	27.0	40.0
21	34.250027268713694	35.0	33.0	38.0	27.0	40.0
22	34.77278322671122	35.0	33.0	39.0	29.0	40.0
23	35.18289256126804	35.0	34.0	39.0	30.0	40.0
24	34.59852142974627	35.0	33.0	39.0	27.0	40.0
25	33.53369633906698	35.0	33.0	38.0	24.0	40.0
26	33.61044175316187	35.0	32.0	38.0	24.0	40.0
27	34.321632313858565	35.0	33.0	39.0	26.0	40.0
28	34.22945886752599	35.0	33.0	39.0	25.0	40.0
29	34.199975761143385	35.0	33.0	39.0	25.0	40.0
30	33.42170243340807	35.0	33.0	39.0	22.0	40.0
31	33.32177947834518	35.0	33.0	39.0	21.0	40.0
32	32.65155951072136	35.0	31.0	39.0	18.0	40.0
33	32.11518304665114	35.0	31.0	39.0	15.0	40.0
34	31.65367348528788	35.0	30.0	39.0	10.0	40.0
35	31.153224200766985	35.0	29.0	40.0	8.0	41.0
36	30.62840793995689	35.0	25.0	40.0	7.0	41.0
37	30.449937238674828	35.0	24.0	40.0	7.0	40.0
38	30.10143961494845	35.0	23.0	39.0	7.0	40.0
39	29.720567535514252	35.0	23.0	39.0	7.0	40.0
40	29.52401637854169	35.0	22.0	39.0	7.0	40.0
41	29.10802565856108	35.0	21.0	39.0	7.0	40.0
42	29.287954153934052	35.0	21.0	39.0	7.0	40.0
43	29.18593453777366	35.0	21.0	39.0	7.0	40.0
44	29.269477219803147	35.0	21.0	39.0	7.0	40.0
45	29.325837755481878	35.0	21.0	39.0	7.0	40.0
46	29.13635915060121	35.0	20.0	39.0	7.0	40.0
47	29.06689231887947	35.0	20.0	39.0	7.0	40.0
48	28.934816520512133	34.0	20.0	38.0	7.0	40.0
49	29.07970082325545	35.0	20.0	38.0	7.0	40.0
50	29.08920764909061	35.0	20.0	38.0	7.0	40.0
51	27.970496117454573	33.0	20.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	8.0
9	32.0
10	44.0
11	45.0
12	57.0
13	62.0
14	103.0
15	187.0
16	326.0
17	635.0
18	1236.0
19	2203.0
20	3507.0
21	5325.0
22	7534.0
23	10683.0
24	15593.0
25	22457.0
26	28426.0
27	30321.0
28	28281.0
29	26178.0
30	26514.0
31	29313.0
32	33648.0
33	38719.0
34	44147.0
35	51297.0
36	56562.0
37	60552.0
38	46851.0
39	6735.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.000597314680956	2.6160651678973657	34.45968991576998	39.92364760165171
2	38.455292294640614	12.726611667546766	34.670914237731246	14.147181800081373
3	12.812313339162202	12.908576226875699	59.12515041076205	15.153960023200048
4	11.403516365556584	3.155379727659132	68.35011297038531	17.090990936398974
5	18.94214704329233	3.5478760701888032	59.92780283421488	17.58217405230399
6	14.667970947998995	11.490256845312812	62.58749794402555	11.254274262662639
7	55.00177463057385	1.3116684124414588	41.04071262238458	2.645844334600102
8	54.96766709661781	8.011115247106487	34.14683553070111	2.8743821255745905
9	51.72173792601955	2.8870209579542405	33.72490629084896	11.666334825177247
10	30.892422760286365	17.761195321900672	39.616852930737465	11.729528987075495
11	23.5731537349481	16.286260896664558	46.39871187790542	13.74187349048192
12	18.732827202922515	15.056312057965492	49.39307634374161	16.817784395370378
13	18.138802081078975	15.323632019529592	53.99690088904663	12.540665010344798
14	15.472008448972879	19.37810019304518	49.313088116900545	15.836803241081398
15	13.309902438602112	15.548533982011307	55.49018759143676	15.651375987949825
16	16.144463585446296	17.047187859795528	47.53932321649627	19.2690253382619
17	16.458183643965825	17.222573300899434	50.233298994953124	16.085944060181617
18	16.76567085364059	16.077633595055275	49.56586476449354	17.5908307868106
19	16.427538803812425	18.21913657730031	46.824276946250336	18.529047672636928
20	17.97293904793234	18.84190205770579	50.24680350078343	12.938355393578435
21	17.80846109230676	20.74517170632894	48.0945661677502	13.3518010336141
22	16.068976860548663	15.128682358440749	50.51446973172779	18.28787104928279
23	16.35187894422466	19.97610741276176	49.646372395405	14.025641247608577
24	17.380818407680255	17.46288425080291	47.657401075166426	17.498896266350407
25	15.887531705290131	22.78816104988876	45.52316974990694	15.801137494914169
26	16.172857674627977	16.38183124561753	48.58194032047231	18.863370759282184
27	17.24837036972913	17.404364725538233	47.14492239237515	18.20234251235749
28	14.513707939091217	18.40491009981215	49.11640710891037	17.96497485218626
29	19.40961070664924	17.315027225430025	45.78079416882363	17.494567899097103
30	16.78713955521698	19.41393907390254	47.0822476345473	16.716673736333178
31	20.67661036903659	17.435355835071896	44.46704814010059	17.420985655790922
32	20.1409316377676	22.478249954552144	41.928374178692316	15.452444228987941
33	17.839105932460157	21.29193105776639	40.57584597937966	20.293117030393795
34	20.823774855648953	20.79676584398833	42.48205891773505	15.897400382627666
35	16.693127418475203	23.42321909329363	37.85693880554377	22.026714682687395
36	22.496429097016023	24.894690824727096	35.298700624150555	17.310179454106322
37	18.422050434135233	25.433659115108597	37.143104478128755	19.001185972627404
38	21.79125150410762	25.625838621155328	34.232364067626406	18.35054580711064
39	20.92419297592562	24.500290000605972	36.24747872607495	18.328038297393455
40	20.82896889635292	24.16008033449622	35.513733909294736	19.497216859856124
41	19.3550732792576	26.48354787607019	35.31532155440325	18.846057290268963
42	18.96344261017859	23.771219820459326	37.2428300596449	20.022507509717187
43	21.319459473497407	23.946432126873102	34.26318204246994	20.470926357159552
44	21.80025450799449	22.683414562358788	34.46349887895288	21.052832050693837
45	19.72385016923916	21.94153241514236	34.44757048746072	23.88704692815776
46	22.082637187600092	24.024862141502982	33.45152661513024	20.440974055766684
47	17.22309270496983	23.172000657911823	40.19546906515924	19.409437571959106
48	19.476613831730393	24.621484283698504	34.6664127357878	21.235489148783294
49	18.853502081944647	22.251616645169108	38.14693941151519	20.747941861371054
50	19.08255927698953	22.139252231273318	36.867300916748185	21.910887574988962
51	18.900421582970473	22.02861916427885	35.173697377875115	23.897261874875557
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	70.0
1	924.5
2	1779.0
3	20039.0
4	38299.0
5	28126.0
6	17953.0
7	16980.0
8	16007.0
9	15649.5
10	15292.0
11	14638.0
12	13984.0
13	13115.5
14	12247.0
15	11419.5
16	10592.0
17	9842.5
18	9093.0
19	8438.5
20	7784.0
21	7283.5
22	6783.0
23	6660.5
24	6538.0
25	6584.0
26	6956.0
27	7282.0
28	7685.0
29	8088.0
30	8575.0
31	9062.0
32	10110.5
33	11159.0
34	12081.5
35	13004.0
36	13340.0
37	13676.0
38	14546.5
39	15417.0
40	16102.0
41	16787.0
42	17336.0
43	17885.0
44	19967.0
45	22049.0
46	44377.5
47	66706.0
48	49249.0
49	31792.0
50	30592.5
51	29393.0
52	27352.0
53	25311.0
54	24813.5
55	24316.0
56	24012.5
57	23709.0
58	22226.0
59	20743.0
60	18523.5
61	16304.0
62	14525.5
63	12747.0
64	10961.5
65	9176.0
66	7948.0
67	6720.0
68	5717.5
69	4715.0
70	3878.5
71	3042.0
72	2482.5
73	1923.0
74	1739.0
75	1287.0
76	1019.0
77	767.0
78	515.0
79	372.0
80	229.0
81	179.0
82	129.0
83	85.0
84	41.0
85	33.5
86	26.0
87	17.5
88	9.0
89	7.0
90	5.0
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	577585.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.4854372267853
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52772220669615	38.559363828419855
2	6.942212996916759	6.731924649539611
3	3.7416004101943736	5.442393954485802
4	2.5292096953884813	4.905193516765399
5	1.8428845814461934	4.4676532344959945
6	1.402190861730822	4.079150220385305
7	1.0383523314158531	3.5241476748904654
8	0.7619131490271276	2.9553353727533684
9	0.5460661263496308	2.382862940171895
>10	1.5755490484588086	10.995642485403819
>50	0.057050704883396586	1.926247224513392
>100	0.03016056352445265	2.601746329256479
>500	0.0010901408502814209	0.30497886476217
>1k	0.002543661983989982	2.366008766546032
>5k	3.633802834271403E-4	1.5537889129622053
>10k+	0.0010901408502814209	7.203562024648229
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18529	3.2080126734593177	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	11563	2.0019564219984938	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	10794	1.8688158452868409	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	8819	1.5268748322757688	No Hit
GAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT	2948	0.5104010665096912	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTC	2225	0.3852246855441191	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1969	0.34090220487027884	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAATCAT	1867	0.3232424664767956	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCT	1725	0.2986573404780249	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTA	1574	0.27251400226806444	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTT	1121	0.19408398763818313	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCGAATCATCGT	632	0.10942112416354303	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	578	0.10007185089640484	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04501501943436897	0.0
2	0.0	0.0	0.0	0.44807257806210343	0.0
3	0.0	0.0	0.0	0.5278876702130423	0.0
4	0.0	0.0	0.0	1.1553277872520928	0.0
5	0.0	0.0	0.0	3.529004388964395	0.0
6	0.0	0.0	0.0	3.825757247850966	0.0
7	0.0	0.0	0.0	4.262922340434741	0.0
8	0.0	0.0	0.0	4.796177186041882	0.0
9	0.0	0.0	0.0	4.913562505951505	0.0
10	0.0	0.0	0.0	7.167083632712068	0.0
11	0.0	0.0	0.0	7.318922755957997	0.0
12	0.0	0.0	0.0	9.229290926876564	0.0
13	0.0	0.0	0.0	9.38718976427712	0.0
14	0.0	0.0	0.0	9.502324333215025	0.0
15	0.0	0.0	0.0	9.871620627266982	0.0
16	0.0	0.0	0.0	10.051334435624193	0.0
17	0.0	0.0	0.0	10.157812270055489	0.0
18	0.0	0.0	0.0	10.238146766276826	0.0
19	0.0	0.0	0.0	10.712708951929153	0.0
20	0.0	0.0	0.0	10.824380827064415	0.0
21	0.0	0.0	0.0	10.914410865933153	0.0
22	0.0	0.0	0.0	11.103647082247635	0.0
23	0.0	0.0	0.0	11.19748608429928	0.0
24	0.0	0.0	0.0	11.28059073556273	0.0
25	0.0	0.0	0.0	11.353480440108383	0.0
26	0.0	0.0	0.0	11.449916462512011	0.0
27	0.0	0.0	0.0	11.583230173913796	0.0
28	0.0	0.0	0.0	11.664776612966056	0.0
29	0.0	0.0	0.0	11.764155925101933	0.0
30	0.0	0.0	0.0	11.878078551208914	0.0
31	0.0	0.0	0.0	11.965338435035536	0.0
32	0.0	0.0	0.0	12.079953599903044	0.0
33	0.0	0.0	0.0	12.173100063194163	0.0
34	0.0	0.0	0.0	12.319745145736125	0.0
35	0.0	0.0	0.0	12.454963338729364	0.0
36	0.0	0.0	0.0	12.577023295272557	0.0
37	0.0	0.0	0.0	12.704969831280245	0.0
38	0.0	0.0	0.0	12.871005999117013	0.0
39	0.0	0.0	0.0	13.171740955876624	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGAC	20	7.0303667E-4	45.000004	30
ACGATAC	20	7.0303667E-4	45.000004	3
CGTTTTT	15110	0.0	44.151226	1
GATACCT	1285	0.0	41.67315	5
CCGATGA	395	0.0	41.582275	18
TGATACC	1295	0.0	41.351353	4
ATACCTG	1310	0.0	40.87786	6
TACGGCT	1030	0.0	40.631065	7
TGATCCG	50	1.0804797E-9	40.5	4
CGATGAA	400	0.0	40.5	19
ACGGCTG	1035	0.0	40.434784	8
GAATCTG	1325	0.0	39.566036	1
TTGTGCG	80	0.0	39.375004	1
CGGCTGT	1085	0.0	39.19355	9
ATGATAC	4190	0.0	39.146782	3
GAATGAT	4330	0.0	39.128178	1
AATGATA	4165	0.0	38.89556	2
CGACCAC	1115	0.0	38.744396	12
TGGGCGA	610	0.0	38.729507	6
CACGACC	215	0.0	38.720932	27
>>END_MODULE