##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933283.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427947
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.906871645320564	31.0	28.0	31.0	19.0	33.0
2	29.404351473430122	31.0	30.0	33.0	16.0	34.0
3	29.6027241691144	31.0	30.0	33.0	16.0	34.0
4	32.26348589895478	35.0	32.0	37.0	19.0	37.0
5	33.574372527439145	35.0	33.0	37.0	28.0	37.0
6	33.1432840982645	35.0	33.0	37.0	26.0	37.0
7	31.788693459704124	35.0	30.0	35.0	23.0	37.0
8	33.17874643355369	35.0	33.0	35.0	28.0	37.0
9	32.75442286077482	35.0	31.0	37.0	25.0	39.0
10	31.05641119110544	35.0	27.0	39.0	15.0	39.0
11	31.93217150721935	35.0	27.0	39.0	15.0	39.0
12	32.82496430632765	35.0	31.0	39.0	21.0	39.0
13	32.96363101038213	35.0	31.0	39.0	22.0	39.0
14	33.27740584698572	36.0	31.0	40.0	18.0	41.0
15	33.94919931673782	37.0	32.0	40.0	23.0	41.0
16	34.15049293487277	37.0	32.0	40.0	24.0	41.0
17	32.14150584067653	35.0	27.0	39.0	18.0	41.0
18	32.885083900576475	36.0	30.0	39.0	21.0	39.0
19	32.67758390641832	36.0	30.0	37.0	19.0	39.0
20	32.46336812736157	34.0	30.0	37.0	24.0	39.0
21	32.73356981121494	35.0	31.0	37.0	24.0	39.0
22	32.88885539564479	35.0	30.0	37.0	25.0	39.0
23	34.02202609201607	35.0	33.0	38.0	27.0	40.0
24	32.13545135262077	34.0	30.0	37.0	21.0	39.0
25	31.292470796617337	34.0	29.0	37.0	18.0	39.0
26	30.544611832773686	33.0	25.0	37.0	18.0	39.0
27	32.250872187443775	34.0	30.0	37.0	22.0	39.0
28	32.40157309199504	35.0	31.0	38.0	21.0	39.0
29	32.57348223027618	35.0	31.0	38.0	21.0	40.0
30	31.488539468672524	35.0	29.0	38.0	18.0	40.0
31	31.80356679682297	35.0	30.0	37.0	20.0	40.0
32	31.566553802223172	35.0	30.0	37.0	18.0	40.0
33	31.521153320387803	35.0	30.0	38.0	15.0	40.0
34	30.73287813677862	35.0	29.0	38.0	10.0	40.0
35	30.13743056967335	35.0	26.0	38.0	9.0	40.0
36	29.595805088013236	34.0	24.0	38.0	8.0	40.0
37	29.645432728819223	35.0	24.0	38.0	8.0	40.0
38	29.564189023407103	34.0	24.0	38.0	8.0	40.0
39	29.078166221518085	34.0	23.0	38.0	7.0	40.0
40	28.95151736079468	34.0	23.0	38.0	7.0	40.0
41	28.471100393272998	33.0	21.0	37.0	7.0	40.0
42	28.863212033265803	34.0	22.0	38.0	7.0	40.0
43	28.85875587397505	34.0	22.0	38.0	7.0	40.0
44	28.847735817753133	34.0	22.0	38.0	7.0	40.0
45	29.045996350015304	34.0	23.0	38.0	7.0	40.0
46	28.84611879508444	34.0	22.0	38.0	7.0	40.0
47	28.908640555956694	34.0	23.0	38.0	7.0	40.0
48	28.63343124265388	34.0	22.0	37.0	7.0	40.0
49	28.373445777163994	33.0	22.0	37.0	7.0	39.0
50	28.575758213049745	34.0	22.0	37.0	7.0	39.0
51	27.077481557295645	31.0	20.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	14.0
10	24.0
11	46.0
12	48.0
13	79.0
14	132.0
15	248.0
16	488.0
17	901.0
18	1518.0
19	2553.0
20	3698.0
21	5252.0
22	7273.0
23	10198.0
24	13463.0
25	18169.0
26	22865.0
27	24205.0
28	23978.0
29	24814.0
30	26921.0
31	30053.0
32	33883.0
33	37649.0
34	41900.0
35	44809.0
36	32181.0
37	17088.0
38	3436.0
39	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.844499435677783	2.6891180449915524	37.75981605198774	41.706566467342924
2	41.18103410001706	7.652816820774536	37.41654924558415	13.749599833624258
3	13.494194374537033	7.6315525053336035	63.59829605067917	15.275957069450188
4	12.16482414878478	3.204368765291029	67.13378058497898	17.49702650094521
5	15.997775425461564	3.6067550420963346	63.01691564609636	17.37855388634574
6	15.133883401449244	7.498825789174828	64.94075200901047	12.426538800365467
7	56.285007255571365	1.3459610652721015	39.55957162919707	2.8094600499594575
8	58.29647129200578	4.843824118407186	34.04860882305519	2.811095766531837
9	54.12936648697152	2.8727856486901415	34.37061131401786	8.627236550320484
10	29.826357002152136	18.31418376574669	40.1811439267012	11.678315305399968
11	21.935660256994442	17.065898347225243	47.57247976969111	13.425961626089212
12	18.635017887729088	15.708720939742538	50.18565383096505	15.470607341563325
13	17.32340687047695	15.722507693709737	53.82302014034448	13.13106529546883
14	15.212631470719504	16.5973823861366	51.54680369298067	16.64318245016322
15	13.69001301563044	15.876732399105498	54.7287397738505	15.704514811413564
16	16.062503066968574	15.790740442157558	49.78303388036369	18.36372261051018
17	16.551348648313926	15.90313753805962	50.913547705673835	16.63196610795262
18	16.536393525366456	15.416628694674808	50.72380458327783	17.323173196680898
19	16.853722540408043	17.697752291755755	48.04870696604953	17.399818201786672
20	17.71925028099274	18.4151308456421	50.18401811439267	13.68160075897249
21	18.321427653424372	18.588049454722196	48.7114058516592	14.379117040194231
22	16.563499685708745	15.901268147691185	50.24173554201805	17.293496624582016
23	16.410443349293256	19.064042977284572	49.4203721488876	15.105141524534579
24	16.322348328180826	17.128990272159868	49.296291363182824	17.25237003647648
25	16.28028704489107	19.13110735675212	47.28272426258392	17.305881335772888
26	16.211820622647195	17.471556057175306	48.687337450665616	17.62928586951188
27	15.7344250573085	17.34233444795734	48.95816538029242	17.965075114441742
28	14.978256653277159	17.868567836671364	49.75569404622535	17.39748146382613
29	17.869268858059524	17.26639046423973	47.321046765136806	17.54329391256394
30	16.88293176491481	18.148275370548223	48.10619071987886	16.8626021446581
31	18.656048529373965	18.0800426221004	46.45575269834816	16.808156150177474
32	18.869860052763542	20.760514736637948	44.43260497211104	15.937020238487476
33	17.35074670461529	20.472161272307083	43.27919111478758	18.897900908290044
34	19.230886067667257	20.95773542050768	43.363781028959195	16.44759748286587
35	16.826149032473648	23.15683951517361	40.01406716252246	20.002944289830282
36	20.717285084367923	23.2680682420954	38.1007461204308	17.913900553105876
37	18.671237326117488	24.685299815164026	38.62721318294088	18.01624967577761
38	20.293634492121686	24.46377705650466	37.90586217452161	17.33672627685204
39	19.52531505069553	23.840335368632097	38.40662511946572	18.227724461206645
40	19.984016712349895	23.603156465637102	37.963813275943046	18.449013546069956
41	18.711896566630916	24.621039521249127	38.26992594877403	18.397137963345926
42	18.404381851023608	23.577686021867194	38.615295819342116	19.402636307767082
43	19.78399194292751	23.446595022280796	37.45276868397255	19.316644350819143
44	20.456738801767507	22.71426134544698	37.05435486170016	19.774644991085346
45	19.034833752777796	22.203684101068593	37.08730286694381	21.67417927920981
46	19.76716742961161	23.366211236438154	36.81554024213279	20.051081091817444
47	17.577877634379956	22.750013436243275	40.802716224205334	18.869392705171435
48	18.75746295686148	23.48141241789287	37.59881480650641	20.162309818739235
49	18.408120631760475	22.273318892292735	38.94500954557457	20.373550930372218
50	18.309276616029553	21.92841636931676	38.95739425676544	20.804912757888243
51	18.173512140522075	22.02282058292265	37.779210977060245	22.024456299495032
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100.0
1	476.5
2	853.0
3	20954.5
4	41056.0
5	27329.0
6	13602.0
7	12672.0
8	11742.0
9	11435.5
10	11129.0
11	10593.0
12	10057.0
13	9421.0
14	8785.0
15	8140.0
16	7495.0
17	6981.0
18	6467.0
19	6016.0
20	5565.0
21	5071.0
22	4577.0
23	4497.5
24	4418.0
25	4393.5
26	4550.0
27	4731.0
28	4996.5
29	5262.0
30	5737.0
31	6212.0
32	7202.0
33	8192.0
34	9001.5
35	9811.0
36	10101.0
37	10391.0
38	11125.5
39	11860.0
40	12294.5
41	12729.0
42	13239.0
43	13749.0
44	15327.0
45	16905.0
46	24871.0
47	32837.0
48	28896.5
49	24956.0
50	24331.5
51	23707.0
52	21603.0
53	19499.0
54	18530.5
55	17562.0
56	17096.5
57	16631.0
58	15833.0
59	15035.0
60	13626.5
61	12218.0
62	11151.5
63	10085.0
64	8747.5
65	7410.0
66	6510.0
67	5610.0
68	4872.5
69	4135.0
70	3515.5
71	2896.0
72	2393.5
73	1891.0
74	1667.0
75	1246.0
76	1049.0
77	766.0
78	483.0
79	355.0
80	227.0
81	170.0
82	113.0
83	78.5
84	44.0
85	44.0
86	44.0
87	26.5
88	9.0
89	6.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	427947.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.681071240801835
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.6135636524729	39.05606056311816
2	7.921359271367895	7.870832285696289
3	3.9871505182065183	5.9425772682845395
4	2.5268871052814417	5.021538331798032
5	1.8291819673224585	4.543785981546855
6	1.3653736777404215	4.069993617248248
7	0.9359930782540178	3.255079716114468
8	0.703161870403814	2.7947067981877876
9	0.5305154201381973	2.3720916944006727
>10	1.5075232409706927	10.663659785895627
>50	0.04949630531930399	1.7389874431800911
>100	0.025949519293590347	2.4394527312467646
>500	0.0014416399607550191	0.5261845585895352
>1k	0.0019221866143400259	2.8455507050039337
>5k	0.0	0.0
>10k+	4.8054665358500647E-4	6.859498519688985
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28732	6.713915508228823	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	4560	1.0655525100070804	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	3180	0.743082671452306	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	3157	0.7377081741430598	No Hit
GAACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCT	1022	0.23881461956737635	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAATCAT	800	0.18693903684334742	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	761	0.17782575879723422	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTC	643	0.1502522508628405	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTA	445	0.103984839244112	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.336737960541843E-4	0.0	0.0	0.046267411618728486	0.0
2	2.336737960541843E-4	0.0	0.0	0.32831168345612893	0.0
3	2.336737960541843E-4	0.0	0.0	0.4339322392726202	0.0
4	2.336737960541843E-4	0.0	0.0	0.8578165053149105	0.0
5	2.336737960541843E-4	0.0	0.0	1.894627138407326	0.0
6	2.336737960541843E-4	0.0	0.0	2.2091520678962584	0.0
7	2.336737960541843E-4	0.0	0.0	2.5038147247205846	0.0
8	2.336737960541843E-4	0.0	0.0	2.922791841045737	0.0
9	2.336737960541843E-4	0.0	0.0	3.148520728034079	0.0
10	2.336737960541843E-4	0.0	0.0	4.652678953234863	0.0
11	2.336737960541843E-4	0.0	0.0	5.078432609645587	0.0
12	2.336737960541843E-4	0.0	0.0	6.242361787791479	0.0
13	2.336737960541843E-4	0.0	0.0	6.395651798003024	0.0
14	2.336737960541843E-4	0.0	0.0	6.49987031104319	0.0
15	2.336737960541843E-4	0.0	0.0	6.740320647182946	0.0
16	2.336737960541843E-4	0.0	0.0	6.880992272407564	0.0
17	2.336737960541843E-4	0.0	0.0	7.035917999191489	0.0
18	2.336737960541843E-4	0.0	0.0	7.177757993396378	0.0
19	2.336737960541843E-4	0.0	0.0	7.533643184786901	0.0
20	2.336737960541843E-4	0.0	0.0	7.693242387491909	0.0
21	2.336737960541843E-4	0.0	0.0	7.857047718525892	0.0
22	2.336737960541843E-4	0.0	0.0	8.077869455797096	0.0
23	2.336737960541843E-4	0.0	0.0	8.248217653120596	0.0
24	2.336737960541843E-4	0.0	0.0	8.391226016305758	0.0
25	2.336737960541843E-4	0.0	0.0	8.514372106826313	0.0
26	2.336737960541843E-4	0.0	0.0	8.637518197346868	0.0
27	2.336737960541843E-4	0.0	0.0	8.785433710249166	0.0
28	2.336737960541843E-4	0.0	0.0	8.906476736605233	0.0
29	2.336737960541843E-4	0.0	0.0	9.049718773586449	0.0
30	2.336737960541843E-4	0.0	0.0	9.220066970909949	0.0
31	2.336737960541843E-4	0.0	0.0	9.359570227154297	0.0
32	2.336737960541843E-4	0.0	0.0	9.49323163849729	0.0
33	2.336737960541843E-4	0.0	0.0	9.628295092616609	0.0
34	2.336737960541843E-4	0.0	0.0	9.782519798012371	0.0
35	2.336737960541843E-4	0.0	0.0	9.964084337546472	0.0
36	2.336737960541843E-4	0.0	0.0	10.10919576489612	0.0
37	2.336737960541843E-4	0.0	0.0	10.26108373233134	0.0
38	2.336737960541843E-4	0.0	0.0	10.46204319693794	0.0
39	2.336737960541843E-4	0.0	0.0	10.82400390702587	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGG	20	7.028319E-4	45.0	2
TACGACA	20	7.028319E-4	45.0	20
CCGCGAA	40	6.7975634E-9	45.0	41
GTACGGG	25	3.886687E-5	45.0	3
CGTTTTT	12580	0.0	44.42766	1
CGGGATA	75	0.0	42.0	6
TCGTTTG	70	0.0	41.785717	1
TGATACC	665	0.0	41.616543	4
TACGGCT	430	0.0	41.337208	7
ACGGCTG	430	0.0	41.337208	8
GATACCT	670	0.0	41.30597	5
GTTTTTT	14090	0.0	40.257275	2
AATGATA	1760	0.0	39.758522	2
ATGATAC	1785	0.0	39.45378	3
GAATGAT	1880	0.0	39.375	1
GTTGGGA	465	0.0	39.19355	4
ATACGGC	1105	0.0	38.687786	6
CGAATAT	35	6.239099E-6	38.57143	14
GATACGG	1115	0.0	38.34081	5
ATACCTG	730	0.0	38.219177	6
>>END_MODULE