##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933280.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	614958
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.47506333765883	31.0	28.0	33.0	25.0	34.0
2	28.700594186920082	31.0	28.0	33.0	16.0	34.0
3	28.69177244624836	31.0	28.0	34.0	16.0	34.0
4	33.178898396313244	35.0	32.0	37.0	28.0	37.0
5	34.25131635005968	35.0	33.0	37.0	30.0	37.0
6	33.73216707482462	35.0	33.0	37.0	28.0	37.0
7	33.64420659622283	35.0	33.0	37.0	29.0	37.0
8	33.61030184175179	35.0	33.0	37.0	28.0	37.0
9	34.760502343249456	37.0	34.0	39.0	28.0	39.0
10	34.53395679054505	37.0	32.0	39.0	27.0	39.0
11	34.93476952897596	37.0	34.0	39.0	27.0	39.0
12	35.06133589610998	37.0	34.0	39.0	27.0	39.0
13	35.26128938886883	37.0	34.0	39.0	30.0	39.0
14	36.064643439064135	38.0	34.0	40.0	29.0	41.0
15	36.40952878082731	38.0	34.0	40.0	30.0	41.0
16	36.30919997788467	38.0	34.0	40.0	30.0	41.0
17	35.595917119543124	38.0	33.0	40.0	27.0	41.0
18	35.35731545894191	38.0	33.0	39.0	27.0	40.0
19	34.71252996139574	37.0	33.0	38.0	27.0	40.0
20	33.94249363371157	35.0	32.0	38.0	27.0	40.0
21	34.27064287317182	35.0	33.0	38.0	27.0	40.0
22	34.77196166242248	35.0	33.0	39.0	29.0	40.0
23	35.09668139938012	35.0	33.0	39.0	30.0	40.0
24	34.55116934815061	35.0	33.0	39.0	27.0	40.0
25	33.55234015981579	35.0	33.0	38.0	24.0	40.0
26	33.566232165448696	35.0	32.0	38.0	24.0	40.0
27	34.36526559537399	35.0	33.0	38.0	27.0	40.0
28	34.082701582872325	35.0	33.0	39.0	25.0	40.0
29	34.00200338884932	35.0	33.0	39.0	24.0	40.0
30	33.32952169091222	35.0	33.0	39.0	21.0	40.0
31	33.24592411189057	35.0	33.0	39.0	21.0	40.0
32	32.56774283772225	35.0	31.0	39.0	17.0	40.0
33	31.77950201477174	35.0	30.0	39.0	12.0	40.0
34	31.117695842642913	35.0	28.0	39.0	8.0	40.0
35	30.656500118707292	35.0	24.0	40.0	7.0	41.0
36	30.062178230057988	35.0	22.0	40.0	7.0	41.0
37	30.017539409195425	35.0	22.0	40.0	7.0	41.0
38	29.729617307198215	35.0	21.0	39.0	7.0	40.0
39	29.41618126766381	35.0	21.0	39.0	7.0	40.0
40	29.1971809456906	35.0	20.0	39.0	7.0	40.0
41	28.881720377651806	35.0	18.0	39.0	7.0	40.0
42	29.027715713918674	35.0	18.0	39.0	7.0	40.0
43	28.939143811447284	35.0	18.0	39.0	7.0	40.0
44	29.031398892282073	35.0	18.0	39.0	7.0	40.0
45	29.12644603371287	35.0	19.0	39.0	7.0	40.0
46	28.88414493347513	35.0	18.0	39.0	7.0	40.0
47	28.800814039332767	35.0	18.0	39.0	7.0	40.0
48	28.66659836931953	35.0	18.0	39.0	7.0	40.0
49	28.80558834912303	35.0	18.0	39.0	7.0	40.0
50	28.76472214362607	35.0	18.0	39.0	7.0	40.0
51	27.64963298306551	33.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	10.0
9	25.0
10	33.0
11	33.0
12	44.0
13	80.0
14	85.0
15	191.0
16	321.0
17	680.0
18	1394.0
19	2384.0
20	4011.0
21	6141.0
22	8863.0
23	12727.0
24	18920.0
25	27733.0
26	34372.0
27	33113.0
28	27992.0
29	24766.0
30	25143.0
31	28272.0
32	32983.0
33	39506.0
34	45825.0
35	54803.0
36	61650.0
37	65094.0
38	50261.0
39	7501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.878284370639946	2.423092308743036	33.49025461901463	39.20836870160239
2	38.79793416786187	15.66903756028867	31.700538898591446	13.832489373258012
3	10.559095092672996	15.828235424207831	59.203555364756625	14.409114118362554
4	9.778553982548402	2.5591991648210124	71.8302713356034	15.83197551702718
5	19.368965035010522	3.0806981940230065	60.79520877848569	16.755127992480787
6	12.678426819392543	12.920394563531168	64.16714637422393	10.234032242852358
7	51.84549188725084	0.9870592788450594	44.719476777275844	2.4479720566282577
8	51.052266984086714	9.15395848171745	36.800399376868015	2.993375157327818
9	48.01596206570205	2.3977247226639866	35.49884707573525	14.087466135898714
10	28.307786873249878	14.30959512682167	44.03780420776703	13.344813792161418
11	21.709612688996646	16.154111337684853	47.62910637799654	14.507169595321956
12	16.175413605482	14.234305432240902	51.98387532156667	17.606405640710424
13	15.966944083986224	14.954191993599563	57.43936984314376	11.639494079270454
14	14.701979647390553	20.517986594206434	49.6144452141447	15.165588544258307
15	12.329785123536892	15.94092604698207	57.85126789146576	13.878020938015279
16	13.843221813522225	17.929029299561922	48.53778632036659	19.68996256654926
17	14.440335762767539	18.059607322776515	52.138194803547556	15.361862110908387
18	14.713037313117319	17.54379973916918	51.162355803160544	16.58080714455296
19	14.61530706162047	19.725086916504868	47.751228539184794	17.908377482689875
20	16.19037397675939	19.129924320034863	52.58554242728771	12.094159275918031
21	16.267777636846745	21.798074014810766	49.83055753401045	12.103590814332035
22	14.429440709772049	15.154368265800267	51.69702646359588	18.719164560831796
23	14.822150455803484	20.79572913922577	50.99958045915331	13.382539945817436
24	16.977094370672468	17.484933930447284	48.19174642821136	17.346225270668892
25	13.663534745462291	25.03699439636528	46.54350378399826	14.755967074174169
26	13.990874173520792	17.242640960846106	50.69110410792281	18.07538075771028
27	17.146699449393292	18.16042721616761	47.20419931117247	17.48867402326663
28	12.768676885250699	19.27432442540791	51.174063919812404	16.782934769528975
29	16.920342527457162	19.817776173332163	46.764982323996115	16.496898975214567
30	13.988434982551654	20.840447640326655	48.29711947807817	16.87399789904351
31	16.558203974905602	16.794968111643396	48.74918287102534	17.897645042425662
32	21.275599309221118	20.74109126151705	43.63940301614094	14.343906413120896
33	15.98320535711382	21.284868234903847	41.728378198185894	21.003548209796442
34	17.955535174759902	23.179957005193852	43.82998513719636	15.034522682849886
35	19.048780567128162	21.86084252908329	38.07154309725217	21.01883380653638
36	16.8510695039336	24.077091443643305	38.417420376676134	20.65441867574696
37	19.577597169237574	22.802370243171076	38.82135040116561	18.79868218642574
38	19.07414815320721	25.62223761622745	36.638437096517166	18.66517713404818
39	22.33941179722843	22.27778157207484	37.57280985042881	17.80999678026792
40	21.42032464005672	24.484273722758303	36.87845348788048	17.216948149304507
41	17.93911128890103	25.020895735968963	36.36215806607931	20.6778349090507
42	20.050800217250607	23.22711469726388	39.181049762748025	17.54103532273749
43	20.462698265572605	22.787572484624967	34.50886076772723	22.2408684820752
44	20.318298160199557	22.836356304007754	34.56122206719808	22.2841234685946
45	18.03489018762257	22.26851264639211	35.92212801524657	23.77446915073875
46	23.102715957837773	24.47581786073195	33.069575483203735	19.351890698226544
47	16.797732528075088	22.982219923962287	40.95108934268682	19.26895820527581
48	19.35335421280803	24.045381961044495	34.07484738795169	22.526416438195778
49	18.728433486514525	21.998087674280196	38.550437590859865	20.723041248345417
50	18.8598245733855	21.557732397984903	36.56184650008619	23.020596528543415
51	18.671031192374112	22.161025631018706	34.29843989345614	24.869503283151044
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	45.0
1	801.5
2	1558.0
3	18833.5
4	36109.0
5	27168.5
6	18228.0
7	17544.5
8	16861.0
9	16649.0
10	16437.0
11	16173.0
12	15909.0
13	15314.5
14	14720.0
15	14125.0
16	13530.0
17	12867.5
18	12205.0
19	11423.0
20	10641.0
21	10083.0
22	9525.0
23	9058.5
24	8592.0
25	8508.0
26	8371.5
27	8319.0
28	8637.5
29	8956.0
30	9580.0
31	10204.0
32	10923.0
33	11642.0
34	12724.5
35	13807.0
36	14747.5
37	15688.0
38	16624.0
39	17560.0
40	18954.5
41	20349.0
42	21351.0
43	22353.0
44	24679.5
45	27006.0
46	58114.0
47	89222.0
48	63737.0
49	38252.0
50	34713.5
51	31175.0
52	27780.0
53	24385.0
54	23055.0
55	21725.0
56	20559.0
57	19393.0
58	17620.0
59	15847.0
60	13969.0
61	12091.0
62	10310.5
63	8530.0
64	7129.5
65	5729.0
66	4735.5
67	3742.0
68	3023.0
69	2304.0
70	1935.0
71	1566.0
72	1229.5
73	893.0
74	760.0
75	508.0
76	389.0
77	302.5
78	216.0
79	169.0
80	122.0
81	75.5
82	29.0
83	26.5
84	24.0
85	19.5
86	15.0
87	10.0
88	5.0
89	4.0
90	3.0
91	3.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	614958.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.66728255826605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.90006281988724	46.4105400135429
2	8.563325847368374	9.705208108626532
3	4.041721456767135	6.871001154372898
4	2.1714849162274614	4.922085972754969
5	1.2351831615728552	3.4997236614030687
6	0.7111446504692579	2.417918090876447
7	0.4092648371885538	1.623434831908857
8	0.2562346281833915	1.1616096061184389
9	0.1592101493039838	0.8119805865077317
>10	0.4849477005156413	4.697718880858224
>50	0.03748775563720162	1.4511308768158842
>100	0.025282433868599283	2.6870288395198236
>500	0.001453013440723925	0.5162612865045105
>1k	0.002034218817013495	2.981470683305953
>5k	0.0	0.0
>10k+	0.0011624107525791399	10.24288740688375
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	18200	2.9595517092224184	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18181	2.956462067328175	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	14946	2.430409881650454	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	10873	1.768088227163481	No Hit
GAACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCT	3787	0.6158144133420493	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTC	3047	0.49548099219784114	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACTGATGCT	2600	0.4227931013174884	No Hit
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	2578	0.41921562122941725	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCT	2306	0.37498495832235695	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTT	1926	0.3131921204374933	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACTGATGCTCGTA	1861	0.30262229290455606	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCACTGATGCTCGT	937	0.15236812920557175	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.049434270307890944	0.0
2	0.0	0.0	0.0	0.5507693208316665	0.0
3	0.0	0.0	0.0	0.6519144396853118	0.0
4	0.0	0.0	0.0	1.4171699530699657	0.0
5	0.0	0.0	0.0	4.969282455061972	0.0
6	0.0	0.0	0.0	5.468828765541712	0.0
7	0.0	0.0	0.0	6.032119266681627	0.0
8	0.0	0.0	0.0	6.745013480595423	0.0
9	0.0	0.0	0.0	6.9006338644265135	0.0
10	0.0	0.0	0.0	9.902790109243233	0.0
11	0.0	0.0	0.0	10.102641155981384	0.0
12	0.0	0.0	0.0	12.356941449659978	0.0
13	0.0	0.0	0.0	12.569313676706377	0.0
14	0.0	0.0	0.0	12.720868742255568	0.0
15	0.0	0.0	0.0	13.14317400537923	0.0
16	0.0	0.0	0.0	13.398475993482482	0.0
17	0.0	0.0	0.0	13.544177000705739	0.0
18	0.0	0.0	0.0	13.644021217709177	0.0
19	0.0	0.0	0.0	14.28260141342986	0.0
20	0.0	0.0	0.0	14.416756916732524	0.0
21	0.0	0.0	0.0	14.521154290211689	0.0
22	0.0	0.0	0.0	14.716289567742837	0.0
23	0.0	0.0	0.0	14.829793254173456	0.0
24	0.0	0.0	0.0	14.931100985758377	0.0
25	0.0	0.0	0.0	15.014846542365495	0.0
26	0.0	0.0	0.0	15.13648086535991	0.0
27	0.0	0.0	0.0	15.30706162046839	0.0
28	0.0	0.0	0.0	15.407393675665656	0.0
29	0.0	0.0	0.0	15.516018980157995	0.0
30	0.0	0.0	0.0	15.631799244826476	0.0
31	0.0	0.0	0.0	15.744002029406886	0.0
32	0.0	0.0	0.0	15.876694018128067	0.0
33	0.0	0.0	0.0	15.997515277466103	0.0
34	0.0	0.0	0.0	16.138663128213633	0.0
35	0.0	0.0	0.0	16.284364135436892	0.0
36	0.0	0.0	0.0	16.42876424080994	0.0
37	0.0	0.0	0.0	16.579506242702752	0.0
38	0.0	0.0	0.0	16.742281586709986	0.0
39	0.0	0.0	0.0	16.954003362831283	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	50	2.1827873E-11	45.0	5
TCGTTAG	20	7.030725E-4	45.0	1
CGACGAA	25	3.888683E-5	45.0	19
CTACGGC	20	7.030725E-4	45.0	6
ACTACCG	25	3.888683E-5	45.0	14
CTACCGA	20	7.030725E-4	45.0	15
CGTTTTT	17555	0.0	44.44888	1
CGCTTTT	355	0.0	43.09859	1
GATACCT	1675	0.0	42.179104	5
TGATACC	1690	0.0	42.071007	4
TACGGCT	1355	0.0	42.011066	7
ACGGCTG	1365	0.0	41.703297	8
CGACCTG	120	0.0	41.250004	12
CGGCTGT	1400	0.0	41.142857	9
AATCTGT	2125	0.0	40.55294	2
CGGTAGT	50	1.0804797E-9	40.5	12
ATACCTG	1760	0.0	40.397728	6
GCGACCT	145	0.0	40.344826	11
GAATCTG	2160	0.0	40.104164	1
CGATGAA	455	0.0	39.56044	19
>>END_MODULE