FastQCFastQC Report
Sat 14 Jan 2017
SRR2933274.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933274.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences757050
Sequences flagged as poor quality0
Sequence length51
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT303284.006076216894525No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC193122.550954362327455RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG166972.2055346410408827No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC125171.653391453668846No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT45610.602470114259296RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC36350.48015322633907936No Hit
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC29570.39059507298064855RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT28410.37527243907271646No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT27780.3669506637606499No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA23730.31345353675450766No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT21010.2775246020738393RNA PCR Primer, Index 38 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGT10420.13763952182814873No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8750.1155802126675913No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA8330.11003236245954691No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT207.0317596E-445.04
CTACGGG502.1827873E-1145.03
CGATGCA253.88954E-545.010
ATACGTC207.0317596E-445.06
CTTACGG253.88954E-545.02
CGTTTTT264850.044.413821
CGATGAA4250.042.8823519
GCGACTG3150.042.14285711
CCGATGA4500.042.018
TCAAGCG1450.041.89655317
TACGGCT14900.041.5268447
CGGCTGT15150.041.4356469
TGATACC20150.041.4267964
ACGGCTG14950.041.2374578
GATACCT20200.040.9900975
ACATACG556.002665E-1140.90909217
GGCACCG1550.040.645168
GAATCTG22100.040.4185521
CGCTTTT7400.040.1351361
GAATGAT63450.040.0709231
Produced by FastQC (version 0.11.5)