##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933273.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	483473
Sequences flagged as poor quality	0
Sequence length	51
%GC	34
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.781356973398722	31.0	28.0	31.0	16.0	33.0
2	29.141583914717057	31.0	30.0	33.0	16.0	34.0
3	29.256202518030996	31.0	30.0	33.0	16.0	34.0
4	32.32557557505797	35.0	32.0	37.0	19.0	37.0
5	33.754236534408335	35.0	33.0	37.0	28.0	37.0
6	33.093107577879216	35.0	33.0	37.0	25.0	37.0
7	32.4558186289617	35.0	32.0	37.0	25.0	37.0
8	33.22512322301349	35.0	33.0	37.0	28.0	37.0
9	33.324864056524355	35.0	32.0	38.0	25.0	39.0
10	32.16505575285486	35.0	28.0	39.0	15.0	39.0
11	32.89137345829033	37.0	31.0	39.0	17.0	39.0
12	33.633185307142284	37.0	31.0	39.0	25.0	39.0
13	33.80085547693459	37.0	32.0	39.0	25.0	39.0
14	34.087545736783646	37.0	32.0	40.0	21.0	41.0
15	34.66934865028657	37.0	32.0	40.0	25.0	41.0
16	34.78671611444693	37.0	32.0	40.0	25.0	41.0
17	32.933892895777014	36.0	30.0	40.0	18.0	41.0
18	33.434559944402274	36.0	31.0	39.0	23.0	39.0
19	33.11720199473393	36.0	32.0	37.0	23.0	39.0
20	32.48595061151295	35.0	31.0	36.0	24.0	39.0
21	32.68627617260943	35.0	31.0	36.0	24.0	39.0
22	32.6056760149998	34.0	30.0	37.0	24.0	39.0
23	33.73072126054609	35.0	32.0	38.0	27.0	39.0
24	31.895431595973303	34.0	30.0	36.0	21.0	39.0
25	31.132538942195325	34.0	28.0	36.0	18.0	39.0
26	29.971965342428636	33.0	25.0	36.0	18.0	39.0
27	31.973950975545687	34.0	30.0	37.0	22.0	39.0
28	32.093204791167246	35.0	31.0	37.0	21.0	39.0
29	32.04597154339539	35.0	30.0	37.0	21.0	39.0
30	31.097343595195596	34.0	27.0	37.0	18.0	39.0
31	31.421365826013034	34.0	30.0	37.0	19.0	39.0
32	31.136717045212453	35.0	30.0	37.0	16.0	39.0
33	30.610046476225147	35.0	27.0	37.0	12.0	40.0
34	29.279463382650118	34.0	23.0	37.0	8.0	39.0
35	28.472011880704816	34.0	21.0	37.0	7.0	39.0
36	27.786308645984366	33.0	18.0	37.0	7.0	40.0
37	27.749210400580797	33.0	18.0	37.0	7.0	40.0
38	27.801798652665195	33.0	18.0	38.0	7.0	40.0
39	27.36834321668428	33.0	17.0	37.0	7.0	40.0
40	27.21422085618018	33.0	16.0	37.0	7.0	40.0
41	26.769852711526806	33.0	15.0	37.0	7.0	40.0
42	27.118296161316145	33.0	15.0	37.0	7.0	40.0
43	27.099881482523326	33.0	15.0	37.0	7.0	40.0
44	27.004207060166753	33.0	15.0	37.0	7.0	40.0
45	27.207364216822864	33.0	15.0	37.0	7.0	40.0
46	26.993234368827213	33.0	15.0	37.0	7.0	40.0
47	26.979289432915593	33.0	15.0	37.0	7.0	40.0
48	26.744105668775713	33.0	15.0	37.0	7.0	40.0
49	26.47118660194054	33.0	12.0	36.0	7.0	39.0
50	26.672610466354893	33.0	15.0	37.0	7.0	39.0
51	25.29634126414505	30.0	12.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	17.0
10	21.0
11	24.0
12	52.0
13	70.0
14	153.0
15	313.0
16	597.0
17	1170.0
18	1913.0
19	3294.0
20	5002.0
21	7124.0
22	10101.0
23	14278.0
24	19526.0
25	26377.0
26	31815.0
27	31296.0
28	28219.0
29	26931.0
30	28831.0
31	31800.0
32	35328.0
33	39553.0
34	43445.0
35	45578.0
36	31510.0
37	15947.0
38	3133.0
39	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.994442295640088	2.2443859326167126	31.72793516907873	47.033236602664466
2	48.10506481230596	9.613360001489225	29.79028818568979	12.491287000515024
3	10.788192929077734	9.62597704525382	66.52574187183153	13.060088153836928
4	10.006970399587981	2.5778068268548604	72.35419558072529	15.061027192831864
5	15.571914046906446	2.914743946404453	66.79545703689762	14.717884969791486
6	12.34608757883067	8.361790627398014	68.73786126629615	10.554260527475163
7	46.43278942153957	1.1510466975405038	49.77134193636459	2.644821944555332
8	47.40409495463035	5.932492610755927	43.40883565369731	3.2545767809164112
9	43.89469525702573	2.25472777176802	43.48701995768119	10.363557013525057
10	24.905010207395243	13.137238273905677	49.79740337102589	12.160348147673188
11	19.49457363699731	14.639700665807604	53.83816676422468	12.027558932970404
12	15.506346786687159	13.077876117177176	57.02758995848786	14.388187137647812
13	14.574133405588316	13.727343615879272	60.68653265022038	11.011990328312026
14	13.208803800832724	15.360940528219777	57.341154521555495	14.089101149392002
15	11.53776943076449	14.16811280050799	61.343032599545374	12.951085169182146
16	12.907649444746655	14.254777412595946	55.53650358965236	17.30106955300503
17	13.300432495713308	15.127008126617206	57.74717512663582	13.825384251033668
18	13.713485551416522	14.110612174826723	56.856122265359176	15.319780008397574
19	13.668808806282872	16.971578557644378	54.005911395258885	15.35370124081386
20	14.694512413309532	16.862368736206573	57.15148519152052	11.291633658963375
21	14.878803986985831	18.171852409545103	54.960876822490604	11.988466780978461
22	13.61213552773371	13.864062729459556	56.45982298908109	16.063978753725646
23	13.621029509403835	18.144549954185653	55.399163965723005	12.835256570687505
24	14.026636440918109	15.744209087167224	54.61649357875207	15.612660893162596
25	13.15854246255737	19.02939771197151	52.96676339733553	14.845296428135594
26	13.463213043954886	15.732833064100788	55.37703242993921	15.426921462005117
27	14.158184634922735	16.454279763295983	53.8596777896594	15.527857812121876
28	12.989349974041984	16.425736287238376	55.69369954475224	14.891214193967398
29	16.695658289087497	15.40478992622132	52.84224765395379	15.057304130737394
30	14.823992239483902	18.226870993830058	54.02411303216519	12.925023734520853
31	16.764534937835204	16.140301526662295	51.53690071627578	15.55826281922672
32	16.63815766340623	17.461574896633316	51.033046312824084	14.867221127136364
33	15.41657962285381	17.291141387419774	49.47018758027853	17.82209140944789
34	18.128830358675664	20.548407046515525	46.59184690768667	14.730915687122135
35	15.106531285097615	23.244524513261343	44.70384075222401	16.945103449417033
36	21.681252107149724	20.484287643777417	41.27552107356564	16.558939175507213
37	17.1339454323199	23.264174007648826	41.76696527003576	17.83491528999551
38	19.73326328460948	21.194151483123154	43.0274286257971	16.045156606470268
39	19.42031923189092	22.454201165318434	40.45706792313118	17.668411679659464
40	19.082554765209224	21.35527733710052	44.30567994489868	15.256487952791572
41	20.09874388021668	23.607109393906175	39.8022226680704	16.491924057806745
42	18.007003493473263	21.872989805014964	43.56872048697652	16.55128621453525
43	21.00923939909778	20.60487348828166	40.22065348013229	18.165233632488267
44	19.798003197696666	22.442411468685947	38.864631530612876	18.894953803004512
45	18.4328804297241	20.947188364189934	39.59642006895938	21.023511137126583
46	19.935963331975103	22.32906491158762	38.10181747481245	19.633154281624826
47	16.665253281982658	21.127550038988733	43.48536526341699	18.721831415611625
48	18.412196751421487	22.824439006935236	38.24867159076101	20.514692650882264
49	17.057415822600227	21.121551772280974	41.234360553743436	20.58667185137536
50	17.97184124035882	20.465672333305065	40.17452887751746	21.38795754881865
51	17.877109993732844	20.635278495386505	38.900827967642456	22.586783543238194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	399.0
2	736.0
3	25575.5
4	50415.0
5	34303.0
6	18191.0
7	17289.0
8	16387.0
9	16129.0
10	15871.0
11	15382.0
12	14893.0
13	14171.5
14	13450.0
15	12624.5
16	11799.0
17	10989.5
18	10180.0
19	9498.0
20	8816.0
21	8196.5
22	7577.0
23	7177.0
24	6777.0
25	6548.0
26	6318.5
27	6318.0
28	6467.0
29	6616.0
30	6889.0
31	7162.0
32	7581.5
33	8001.0
34	8725.0
35	9449.0
36	10006.0
37	10563.0
38	11088.5
39	11614.0
40	12162.5
41	12711.0
42	13672.5
43	14634.0
44	16285.5
45	17937.0
46	30888.5
47	43840.0
48	36493.5
49	29147.0
50	27430.5
51	25714.0
52	22615.0
53	19516.0
54	18134.0
55	16752.0
56	15549.5
57	14347.0
58	13492.5
59	12638.0
60	11089.5
61	9541.0
62	8420.5
63	7300.0
64	6444.0
65	5588.0
66	4804.0
67	4020.0
68	3420.5
69	2821.0
70	2400.5
71	1980.0
72	1668.0
73	1356.0
74	1174.5
75	847.0
76	701.0
77	549.0
78	397.0
79	298.0
80	199.0
81	145.5
82	92.0
83	61.0
84	30.0
85	22.5
86	15.0
87	10.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	483473.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.24222703123755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.64472263268114	46.48123253240908
2	8.380447288841243	9.426700380846567
3	3.6156836015927625	6.100622939817083
4	1.9652290551678613	4.421154347565412
5	1.1324963595769584	3.184705868368866
6	0.7048054890539904	2.3783898196942164
7	0.471127031653588	1.8548063432369895
8	0.2710998750154634	1.2197808575007854
9	0.16725809815609669	0.8466271136377382
>10	0.5595995978717804	5.27447087963436
>50	0.045635805085444	1.78256592903516
>100	0.03628420566478923	3.8478851445481577
>500	0.002618447831479635	0.9427213413187694
>1k	0.001870319879628311	2.179766975542015
>5k	7.481279518513243E-4	2.6344022843101165
>10k+	3.7406397592566217E-4	7.424167242534714
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35289	7.299063236209674	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	7273	1.5043239229491616	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	5249	1.0856862741042417	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	4673	0.9665482870811812	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	1885	0.38988733600428566	RNA PCR Primer, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1403	0.29019200658568317	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	1266	0.26185536731110115	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	1134	0.23455291195164985	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	733	0.1516113619581652	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTT	712	0.1472677895146161	RNA PCR Primer, Index 38 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	659	0.13630544001423037	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCC	651	0.1346507457500212	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGAGCTATGTCGTATGCCG	613	0.12679094799502763	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	579	0.11975849737213867	No Hit
GAATCTTTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	534	0.1104508421359621	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06515358665323606	0.0
2	0.0	0.0	0.0	0.4755177641771102	0.0
3	0.0	0.0	0.0	0.6296111675315892	0.0
4	0.0	0.0	0.0	1.2991418341872245	0.0
5	0.0	0.0	0.0	2.839869030948988	0.0
6	0.0	0.0	0.0	3.385090791005909	0.0
7	0.0	0.0	0.0	3.892461419769046	0.0
8	0.0	0.0	0.0	4.549995553009165	0.0
9	0.0	0.0	0.0	4.9084436979934765	0.0
10	0.0	0.0	0.0	7.1131169682691695	0.0
11	0.0	0.0	0.0	7.695776186053823	0.0
12	0.0	0.0	0.0	9.213337663116658	0.0
13	0.0	0.0	0.0	9.447270064719229	0.0
14	0.0	0.0	0.0	9.599295100243447	0.0
15	0.0	0.0	0.0	9.900656293112542	0.0
16	0.0	0.0	0.0	10.095910216289225	0.0
17	0.0	0.0	0.0	10.267377909417899	0.0
18	0.0	0.0	0.0	10.442775501424071	0.0
19	0.0	0.0	0.0	10.902987343657246	0.0
20	0.0	0.0	0.0	11.075282383918026	0.0
21	0.0	0.0	0.0	11.260194467943402	0.0
22	0.0	0.0	0.0	11.514810547848587	0.0
23	0.0	0.0	0.0	11.70303202040238	0.0
24	0.0	0.0	0.0	11.855884403058703	0.0
25	0.0	0.0	0.0	11.984123208534912	0.0
26	0.0	0.0	0.0	12.125185894558745	0.0
27	0.0	0.0	0.0	12.28052031861138	0.0
28	0.0	0.0	0.0	12.42282402533337	0.0
29	0.0	0.0	0.0	12.585190900008895	0.0
30	0.0	0.0	0.0	12.76948247368519	0.0
31	0.0	0.0	0.0	12.920886998860329	0.0
32	0.0	0.0	0.0	13.082840199969802	0.0
33	0.0	0.0	0.0	13.23858829758849	0.0
34	0.0	0.0	0.0	13.412331195330452	0.0
35	0.0	0.0	0.0	13.601173178233324	0.0
36	0.0	0.0	0.0	13.776777607022522	0.0
37	0.0	0.0	0.0	13.95031366798146	0.0
38	0.0	0.0	0.0	14.146188101507219	0.0
39	0.0	0.0	0.0	14.494708080906276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGG	45	3.8380676E-10	45.000004	3
CGTTGAT	20	7.0292276E-4	45.0	25
GCACGGG	40	6.8012014E-9	45.0	3
TCTAGCG	30	2.162882E-6	44.999996	1
AGGGCGA	30	2.162882E-6	44.999996	6
CTAGCGG	30	2.162882E-6	44.999996	2
CGTTTTT	18030	0.0	44.463394	1
CCGATGA	140	0.0	43.39286	18
GATACCT	1040	0.0	42.187504	5
TACGGCT	750	0.0	42.0	7
TGATACC	1040	0.0	41.971157	4
CGATGAA	140	0.0	41.785717	19
ACGGCTG	760	0.0	41.44737	8
CGACCAC	605	0.0	40.909092	12
GAATGAT	3025	0.0	40.834713	1
CGGCTGT	730	0.0	40.684933	9
ATGATAC	2870	0.0	40.53136	3
ATACCTG	1085	0.0	40.437794	6
GTTTTTT	20000	0.0	40.41	2
ACCACCG	335	0.0	40.298508	14
>>END_MODULE