SRR2933272.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933272.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	857031
Sequences flagged as poor quality	0
Sequence length	51
%GC	36

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	22298	2.6017728646921756	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	20427	2.3834610416659374	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20042	2.3385385126092286	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	19155	2.2350416729383182	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	5067	0.5912271551437462	RNA PCR Primer, Index 33 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	4099	0.47827908208687897	RNA PCR Primer, Index 33 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCT	3984	0.4648606643166933	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTC	3929	0.458443160165735	RNA PCR Primer, Index 33 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTA	3440	0.40138571416903235	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	3435	0.40080230470076345	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	1925	0.22461264528354283	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGT	1493	0.1742060672251062	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTAT	987	0.11516502903628922	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCC	25	3.8899714E-5	45.000004	33
ACGGGCG	20	7.0322823E-4	45.0	5
GCGATCG	35	1.211447E-7	45.0	9
CGCCCAA	35	1.211447E-7	45.0	37
CGTTTTT	22565	0.0	44.421673	1
CGGCTGT	2165	0.0	42.713627	9
ACGGCTG	2135	0.0	42.576115	8
GATACCT	2495	0.0	41.753506	5
TGATACC	2515	0.0	41.510937	4
CGTTAGG	60	3.6379788E-12	41.249996	2
TACGGCT	2210	0.0	41.23303	7
CGATGAA	760	0.0	41.151314	19
CGCTTTT	580	0.0	41.12069	1
TGATCCG	55	6.002665E-11	40.909092	4
GCGACTG	485	0.0	40.824745	11
GAATCTG	2655	0.0	40.508472	1
TGTTGCG	50	1.0804797E-9	40.500004	1
CCGATGA	750	0.0	40.5	18
ATACCTG	2595	0.0	40.40462	6
ACCGGTG	90	0.0	40.0	17