SRR2933271.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933271.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	494235
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20169	4.080852226167714	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	9353	1.892419597964531	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	7909	1.6002508927939136	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	6829	1.3817313626109038	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGTGATCT	2258	0.45686768440114517	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	2112	0.42732708124677526	RNA PCR Primer, Index 33 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	2095	0.42388742197537604	RNA PCR Primer, Index 33 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTA	1159	0.23450382915010068	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTC	1130	0.2286361750988902	RNA PCR Primer, Index 33 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCT	850	0.17198296356996165	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGGTGATCTCGTATGCCG	781	0.15802199358604713	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	693	0.14021669853409816	RNA PCR Primer, Index 33 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTT	646	0.13070705231317087	Illumina PCR Primer Index 5 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGT	545	0.11027142958309306	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCCC	35	1.2100463E-7	45.0	35
CACGACC	20	7.029379E-4	45.0	27
ACGTGAG	20	7.029379E-4	45.0	44
GTTCCCG	20	7.029379E-4	45.0	1
TCCCGCG	20	7.029379E-4	45.0	1
ACCGGTG	120	0.0	44.999996	17
CACCGGT	130	0.0	44.999996	16
CGTTTTT	15335	0.0	44.54516	1
TGATCCG	140	0.0	43.392857	4
CCGGTGT	110	0.0	42.954544	18
TACGGCT	1100	0.0	42.340904	7
ACGGCTG	1110	0.0	41.959457	8
AATGATC	325	0.0	41.538464	2
CGTAGGG	65	0.0	41.53846	3
AGGGCGA	65	0.0	41.53846	6
GAATGAT	3800	0.0	41.328953	1
TTGCTCG	60	3.6379788E-12	41.249996	1
CGGCTGT	1075	0.0	41.23256	9
TGATACC	1265	0.0	41.08696	4
GATACCT	1260	0.0	41.07143	5