##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933270.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	741288
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.42375298129742	31.0	28.0	33.0	25.0	34.0
2	28.50058681646	31.0	27.0	33.0	16.0	34.0
3	28.489867635790677	31.0	26.0	33.0	16.0	34.0
4	33.14178834676941	35.0	32.0	37.0	28.0	37.0
5	34.292292604224	35.0	33.0	37.0	30.0	37.0
6	33.66692972232115	35.0	33.0	37.0	28.0	37.0
7	33.79028663623315	35.0	33.0	37.0	29.0	37.0
8	33.574311198886264	35.0	33.0	37.0	28.0	37.0
9	34.86554618447891	37.0	34.0	39.0	28.0	39.0
10	34.758343585758844	37.0	33.0	39.0	28.0	39.0
11	35.25734802128188	37.0	34.0	39.0	30.0	39.0
12	35.37805684160542	37.0	34.0	39.0	30.0	39.0
13	35.5733156344093	37.0	34.0	39.0	30.0	39.0
14	36.42311490270988	38.0	35.0	40.0	30.0	41.0
15	36.65454856951684	38.0	35.0	40.0	30.0	41.0
16	36.58540000647522	38.0	34.0	41.0	31.0	41.0
17	35.835676552163264	38.0	34.0	40.0	27.0	41.0
18	35.53868267124248	38.0	34.0	39.0	28.0	40.0
19	34.8596969059259	37.0	34.0	38.0	27.0	40.0
20	33.8446379814593	35.0	32.0	37.0	27.0	40.0
21	34.324482522312515	35.0	33.0	38.0	28.0	40.0
22	34.71400589244666	35.0	33.0	38.0	29.0	40.0
23	34.98551709996654	35.0	33.0	39.0	30.0	40.0
24	34.465754200796454	35.0	33.0	38.0	27.0	40.0
25	33.53067633632273	35.0	33.0	38.0	24.0	40.0
26	33.444152610051695	35.0	32.0	38.0	24.0	40.0
27	34.25549044366022	35.0	33.0	38.0	27.0	40.0
28	33.841751114276775	35.0	33.0	39.0	24.0	40.0
29	33.80519717033056	35.0	33.0	39.0	24.0	40.0
30	33.01010538414219	35.0	32.0	38.0	21.0	40.0
31	32.79758339538749	35.0	32.0	38.0	20.0	40.0
32	31.931122586632995	35.0	31.0	38.0	15.0	40.0
33	30.941538241547146	35.0	27.0	39.0	10.0	40.0
34	30.14137959875244	35.0	23.0	39.0	7.0	40.0
35	29.532841486709618	35.0	20.0	39.0	7.0	40.0
36	28.851685174992717	35.0	18.0	39.0	7.0	40.0
37	28.729076957943473	35.0	15.0	39.0	7.0	40.0
38	28.532421946665803	35.0	15.0	39.0	7.0	40.0
39	28.206798167513842	35.0	15.0	39.0	7.0	40.0
40	28.078668479727177	35.0	15.0	39.0	7.0	40.0
41	27.69423489925643	34.0	15.0	39.0	7.0	40.0
42	27.88073731127443	35.0	15.0	39.0	7.0	40.0
43	27.861733900993947	35.0	15.0	39.0	7.0	40.0
44	27.902515621458868	35.0	13.0	39.0	7.0	40.0
45	28.044098919718113	35.0	13.0	39.0	7.0	40.0
46	27.80869918304357	35.0	12.0	39.0	7.0	40.0
47	27.71504732303774	35.0	12.0	39.0	7.0	40.0
48	27.591950766773508	34.0	12.0	38.0	7.0	40.0
49	27.707375540950345	35.0	12.0	39.0	7.0	40.0
50	27.728075997453082	35.0	12.0	39.0	7.0	40.0
51	26.533690009820745	33.0	10.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	16.0
10	22.0
11	33.0
12	64.0
13	91.0
14	141.0
15	225.0
16	371.0
17	881.0
18	1818.0
19	3459.0
20	5545.0
21	8551.0
22	12493.0
23	18513.0
24	27518.0
25	39885.0
26	48038.0
27	43861.0
28	34926.0
29	29205.0
30	28684.0
31	31570.0
32	37567.0
33	44145.0
34	52673.0
35	62619.0
36	70477.0
37	72831.0
38	56860.0
39	8200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.460970634894938	2.0948673120298724	29.09409028609663	42.35007176697856
2	41.53824154714497	17.815073223902182	28.196733253472335	12.449951975480515
3	9.305290251562145	17.905591349111276	59.86000043168107	12.929117967645503
4	8.667616365029517	2.252430904048089	75.06043535036315	14.019517380559243
5	19.812542493605726	2.731192195206182	62.138197299834886	15.318068011353212
6	10.925308382167255	14.503135083800089	65.4026505217945	9.168906012238159
7	46.32032354496498	0.7992844886198077	50.496028534119	2.3843634322962193
8	45.163957867927174	10.586708539730846	40.98434076903983	3.2649928233021446
9	42.47593378012324	2.0535878093264697	39.68147332750564	15.789005083044646
10	25.403217103204152	11.087458585596961	49.22594187414338	14.283382437055502
11	19.754265548612686	14.83202210207099	50.862687646366865	14.55102470294946
12	13.729886359957263	12.761436850454885	55.654347568016746	17.854329221571103
13	14.051488760104036	13.87544382210423	61.71960155836867	10.353465859423059
14	13.097743387185549	20.174614994442106	52.05075490227819	14.67688671609415
15	10.341324829216175	15.07133529748222	61.404069673325345	13.183270199976258
16	11.051844896990104	16.63698859282762	51.560661982927016	20.750504527255266
17	11.763174366777825	17.56456330063349	55.83066230668782	14.841600025900863
18	12.075603544101618	16.723459707967752	54.583778504440915	16.617158243489712
19	12.334477288179494	19.11564735973063	50.62836576337402	17.921509588715857
20	14.21228996017742	17.135445332987988	57.00591403071411	11.646350676120482
21	14.329248551170396	21.16910026872147	52.7367231089671	11.764928071141041
22	12.370900378800142	13.816087674426134	54.48030994701115	19.332701999762573
23	12.992521125392559	20.54909832615664	53.473413841853635	12.984966706597165
24	15.503690873182894	15.832442991118162	51.144224646830914	17.519641488868025
25	11.34471352564725	24.981383753682778	49.53918045348097	14.134722267189002
26	12.527384768133304	15.258037361996957	55.13538597684031	17.07919189302943
27	16.919065194634204	17.104688056463885	49.588688876657926	16.387557872243985
28	11.566489677426317	16.920144397319262	55.76294233820054	15.750423587053886
29	16.286787321526855	14.667038991593012	53.36778687905376	15.678386807826378
30	15.944814970699648	19.390169542741823	51.258889932118144	13.40612555444038
31	14.43501041430591	15.757438404506752	51.93069360356568	17.876857577621653
32	16.16901932852009	22.093707169143435	46.35903994129137	15.378233561045098
33	13.955035020127129	16.84986132245497	43.38934395268775	25.805759704730146
34	15.524465524870227	19.088802192939855	48.1876139907836	17.19911829140631
35	17.24107229578787	20.03243004068594	38.47802743333225	24.248470230193934
36	15.385248378497964	19.347271236010837	42.109004867204106	23.158475518287087
37	17.395802980757814	24.866583568059916	39.95909821823636	17.7785152329459
38	17.100506146059292	22.024098595957305	37.14939402769234	23.72600123029106
39	19.083675980185838	24.08591532575733	39.06335998963966	17.767048704417178
40	17.59032926473921	21.890142562674697	37.38762802041852	23.131900152167578
41	17.196420284693666	24.539720054823498	37.19957695254746	21.064282707935376
42	16.753407582478065	23.019797973257358	39.94965519474212	20.277139249522453
43	19.53181489515546	21.589854415557785	35.46583783900454	23.412492850282213
44	20.644742664119747	22.543599788476275	34.925696895133875	21.885960652270104
45	16.68420371029883	21.652717971962314	36.978070601439654	24.685007716299197
46	22.174377569851394	24.878184996924272	33.50276815488717	19.444669278337166
47	16.396191493724434	20.947593917613666	42.18009734408219	20.476117244579704
48	19.7804362137253	22.952617606112604	34.23514207703349	23.031804103128607
49	16.960344697337607	21.209435469075448	39.501920980779396	22.328298852807546
50	18.472172758765822	20.399898554947605	37.18136540723713	23.94656327904944
51	18.90331423144581	20.83953874877241	34.846915099124764	25.41023192065702
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	812.0
2	1562.0
3	20614.0
4	39666.0
5	31048.0
6	22430.0
7	22039.5
8	21649.0
9	21815.0
10	21981.0
11	21876.0
12	21771.0
13	21355.5
14	20940.0
15	20189.5
16	19439.0
17	18758.0
18	18077.0
19	17257.0
20	16437.0
21	15376.0
22	14315.0
23	13636.0
24	12957.0
25	12529.5
26	11605.0
27	11108.0
28	11188.5
29	11269.0
30	11636.0
31	12003.0
32	12625.0
33	13247.0
34	14372.0
35	15497.0
36	16362.5
37	17228.0
38	18533.5
39	19839.0
40	21103.5
41	22368.0
42	23828.0
43	25288.0
44	28001.0
45	30714.0
46	76209.0
47	121704.0
48	83581.5
49	45459.0
50	41677.5
51	37896.0
52	32557.5
53	27219.0
54	24978.0
55	22737.0
56	20653.0
57	18569.0
58	16462.0
59	14355.0
60	12659.0
61	10963.0
62	9251.5
63	7540.0
64	6200.5
65	4861.0
66	3964.0
67	3067.0
68	2523.0
69	1979.0
70	1576.0
71	1173.0
72	943.0
73	713.0
74	605.0
75	378.5
76	260.0
77	230.5
78	201.0
79	138.0
80	75.0
81	57.0
82	39.0
83	28.0
84	17.0
85	10.0
86	3.0
87	4.5
88	6.0
89	3.5
90	1.0
91	3.0
92	5.0
93	2.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	741288.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.97936425871569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.75351702362394	45.209616033667416
2	6.640496368622017	7.1689954468105315
3	3.3003028074933223	5.344447422292323
4	2.0523274874644244	4.431333320960676
5	1.3178120791389485	3.556732912218838
6	0.8611828503622644	2.789166166383823
7	0.5791630358552139	2.1883996734328544
8	0.4175366547723391	1.8030690543457368
9	0.2711520398689258	1.3172953256620688
>10	0.7367933202836796	6.078475295689885
>50	0.03991010886589164	1.5082976261415533
>100	0.02525951156703411	2.778933811251651
>500	0.0015155706940025734	0.49085725530839663
>1k	0.0017681658096696691	3.309332345441693
>5k	2.525951156670956E-4	0.739149216951894
>10k+	0.0010103804626683823	11.285899093440724
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	24513	3.3068119273480754	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCG	23232	3.1340045974034383	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19349	2.610186594144246	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	15678	2.1149674620390453	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	5421	0.731294719461262	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTC	4826	0.6510290197602011	No Hit
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	4334	0.5846580546292399	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTACTCCT	4022	0.542569149912045	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCT	3852	0.519636092854599	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTACTCCTCGTA	2955	0.39863049179266363	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTT	2843	0.3835216542018757	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTACTCCTCGT	1439	0.19412158297449844	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTAT	767	0.10346855742977087	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06556156311716904	0.0
2	0.0	0.0	0.0	0.723605400330236	0.0
3	0.0	0.0	0.0	0.8388102869599939	0.0
4	0.0	0.0	0.0	1.7180906746095985	0.0
5	0.0	0.0	0.0	5.567741552540983	0.0
6	0.0	0.0	0.0	6.108826798761076	0.0
7	0.0	0.0	0.0	6.792231899072965	0.0
8	0.0	0.0	0.0	7.644397319260531	0.0
9	0.0	0.0	0.0	7.785637970667271	0.0
10	0.0	0.0	0.0	11.445753877035646	0.0
11	0.0	0.0	0.0	11.621798815035453	0.0
12	0.0	0.0	0.0	14.205275142724554	0.0
13	0.0	0.0	0.0	14.399531626034685	0.0
14	0.0	0.0	0.0	14.56491943751956	0.0
15	0.0	0.0	0.0	15.086713935744273	0.0
16	0.0	0.0	0.0	15.388620886888766	0.0
17	0.0	0.0	0.0	15.546454279578247	0.0
18	0.0	0.0	0.0	15.670157887352824	0.0
19	0.0	0.0	0.0	16.429242075954285	0.0
20	0.0	0.0	0.0	16.581004953540326	0.0
21	0.0	0.0	0.0	16.690274225402273	0.0
22	0.0	0.0	0.0	16.922437703025004	0.0
23	1.3490033563203505E-4	0.0	0.0	17.04290370274441	0.0
24	1.3490033563203505E-4	0.0	0.0	17.153791778633945	0.0
25	1.3490033563203505E-4	0.0	0.0	17.24350050182925	0.0
26	1.3490033563203505E-4	0.0	0.0	17.365990006583136	0.0
27	1.3490033563203505E-4	0.0	0.0	17.532861721759964	0.0
28	1.3490033563203505E-4	0.0	0.0	17.630259764086293	0.0
29	1.3490033563203505E-4	0.0	0.0	17.737235730242496	0.0
30	1.3490033563203505E-4	0.0	0.0	17.856487626941217	0.0
31	1.3490033563203505E-4	0.0	0.0	17.970613310885916	0.0
32	1.3490033563203505E-4	0.0	0.0	18.09593572268808	0.0
33	1.3490033563203505E-4	0.0	0.0	18.21613192173622	0.0
34	1.3490033563203505E-4	0.0	0.0	18.37841702550156	0.0
35	1.3490033563203505E-4	0.0	0.0	18.508326048715208	0.0
36	1.3490033563203505E-4	0.0	0.0	18.633918261188633	0.0
37	1.3490033563203505E-4	0.0	0.0	18.776373015616063	0.0
38	1.3490033563203505E-4	0.0	0.0	18.92611238816762	0.0
39	1.3490033563203505E-4	0.0	0.0	19.08043837213067	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACGACG	30	2.1644537E-6	45.000004	26
CGATAGG	25	3.88946E-5	45.000004	10
ACGACGG	30	2.1644537E-6	45.000004	27
CGTTTTT	23995	0.0	44.521774	1
CGATGAA	605	0.0	42.768597	19
ACGGCTG	1990	0.0	42.738693	8
CGGCTGT	2005	0.0	42.531174	9
TACGGCT	2020	0.0	42.103962	7
GATACCT	2695	0.0	41.743973	5
CCGATGA	615	0.0	41.707317	18
ATACCTG	2710	0.0	41.512913	6
TGATACC	2715	0.0	41.436462	4
CGCTTTT	640	0.0	41.132812	1
CGGGTAT	115	0.0	41.086956	6
GAATCTG	2855	0.0	40.980732	1
TACCTGT	2795	0.0	40.73345	7
CGACCAC	1760	0.0	40.653408	12
GCGGGTA	135	0.0	40.0	5
GCGACCA	1820	0.0	39.931316	11
GAATGAT	8195	0.0	39.838318	1
>>END_MODULE