##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933267.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	468755
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.68278738360124	31.0	28.0	31.0	16.0	33.0
2	29.128034900961058	31.0	30.0	33.0	16.0	34.0
3	29.373079753815958	31.0	30.0	33.0	16.0	34.0
4	32.09337500399996	35.0	30.0	37.0	19.0	37.0
5	33.53566361958806	35.0	33.0	37.0	28.0	37.0
6	32.898593081673795	35.0	33.0	37.0	25.0	37.0
7	31.541950485861484	33.0	30.0	35.0	23.0	37.0
8	33.01051295452849	35.0	32.0	35.0	28.0	37.0
9	32.474516538490256	34.0	31.0	37.0	25.0	39.0
10	30.459114036116947	34.0	27.0	37.0	15.0	39.0
11	31.242832609785495	35.0	27.0	39.0	15.0	39.0
12	32.117822743224075	35.0	30.0	39.0	18.0	39.0
13	32.340030506341265	35.0	30.0	39.0	21.0	39.0
14	32.57888875851991	36.0	30.0	39.0	17.0	41.0
15	33.23891798487483	36.0	30.0	39.0	21.0	41.0
16	33.4191592623012	36.0	31.0	39.0	22.0	41.0
17	31.428893558468708	34.0	27.0	38.0	17.0	41.0
18	32.33572548559482	34.0	29.0	39.0	19.0	39.0
19	32.23187805996736	35.0	30.0	37.0	18.0	39.0
20	32.31442224616271	34.0	30.0	37.0	23.0	39.0
21	32.61336732408188	35.0	31.0	37.0	23.0	39.0
22	33.22536932939382	35.0	31.0	38.0	25.0	39.0
23	34.33043487536133	35.0	33.0	39.0	27.0	40.0
24	32.4633891905153	35.0	31.0	37.0	22.0	39.0
25	31.440793164872908	34.0	29.0	37.0	18.0	39.0
26	31.278034367633413	34.0	27.0	37.0	20.0	39.0
27	32.744975520261114	35.0	31.0	38.0	22.0	40.0
28	32.699390939829975	35.0	31.0	38.0	22.0	40.0
29	33.174148542415544	35.0	31.0	38.0	22.0	40.0
30	31.93549935467355	35.0	30.0	38.0	18.0	40.0
31	32.21546436838007	35.0	30.0	38.0	21.0	40.0
32	32.17374108009515	35.0	30.0	38.0	20.0	40.0
33	32.47930795404849	35.0	31.0	38.0	18.0	40.0
34	31.84366886753208	35.0	31.0	38.0	15.0	40.0
35	31.44434939360647	35.0	30.0	38.0	12.0	40.0
36	30.8848780279677	35.0	29.0	38.0	10.0	40.0
37	31.15959723096287	35.0	30.0	38.0	10.0	40.0
38	31.08748706680462	35.0	29.0	38.0	10.0	40.0
39	30.57859649497072	35.0	28.0	38.0	10.0	40.0
40	30.352486906806327	35.0	26.0	38.0	10.0	40.0
41	29.852950901857046	34.0	25.0	38.0	8.0	40.0
42	30.348757879915947	35.0	27.0	38.0	9.0	40.0
43	30.34912480933537	35.0	27.0	38.0	8.0	40.0
44	30.363964117716076	35.0	27.0	38.0	8.0	40.0
45	30.693950997856025	35.0	28.0	38.0	8.0	40.0
46	30.449804268754466	35.0	27.0	38.0	8.0	40.0
47	30.52897995754712	35.0	28.0	38.0	8.0	40.0
48	30.28921291506224	34.0	27.0	38.0	8.0	40.0
49	30.089163848918943	34.0	27.0	38.0	8.0	40.0
50	30.33363484122836	34.0	27.0	38.0	8.0	40.0
51	28.547232562852663	33.0	24.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	31.0
10	35.0
11	26.0
12	45.0
13	70.0
14	128.0
15	225.0
16	459.0
17	822.0
18	1401.0
19	2254.0
20	3174.0
21	4779.0
22	6589.0
23	8956.0
24	12252.0
25	16567.0
26	20742.0
27	23268.0
28	24103.0
29	26166.0
30	29643.0
31	34663.0
32	39851.0
33	44728.0
34	49886.0
35	52666.0
36	40026.0
37	20789.0
38	4332.0
39	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.973525615726768	2.8247165363569455	41.45363782786317	33.74812002005312
2	33.30545807511386	9.387419867521412	41.29513285191624	16.011989205448476
3	15.227144243794733	9.372273362417467	59.509978560228696	15.890603833559108
4	13.195165918230206	3.4043370204051153	64.20774178408763	19.192755277277044
5	18.586681742061415	3.8245992042751547	58.91393158472976	18.674787468933665
6	16.170067519279794	9.04374353340231	60.68031274333073	14.105876203987158
7	63.16049962133737	1.251186654009024	32.58354577551173	3.0047679491418755
8	65.47087497733357	5.982656185000693	25.436102014911842	3.1103668227538908
9	61.3941184627364	2.434320700579194	25.971562969994988	10.199997866689422
10	32.78450363196126	18.949557871382705	33.34087103070901	14.92506746594703
11	25.730712205736474	19.166728888225194	39.762135870550715	15.340423035487621
12	21.939819308594043	17.039178248765346	42.73959744429393	18.281404998346684
13	18.971530970336318	18.423056820727247	46.92941941952619	15.675992789410245
14	17.03725826924513	18.986250813324656	44.5059786028949	19.470512314535313
15	16.17902742370748	17.15181704728483	48.20407248989344	18.465083039114248
16	17.885249224008277	17.211763074527205	42.6952245842711	22.207763117193416
17	18.49836268413137	17.853462896395772	44.97701357852183	18.67116084095103
18	18.96662435600687	16.39427846102975	44.42000618660067	20.219090996362706
19	18.16769954453819	20.536740941429958	41.17225416262226	20.123305351409584
20	19.661870273383748	20.377595972309628	44.697123230685534	15.263410523621083
21	19.681710061759343	22.001471984298835	42.02813836652409	16.288679587417736
22	18.160872950688525	17.249096009642564	44.465445701912515	20.124585337756397
23	18.341777687704663	21.53065033972971	42.90215571033909	17.22541626222654
24	18.09047370161385	19.38389990506768	43.269298460816415	19.256327932502053
25	17.77709037770264	22.32274855734872	40.133545242184084	19.766615822764557
26	16.9508591908353	19.79072223229619	42.44861388145193	20.80980469541658
27	17.07373788012928	19.73568281938326	42.27176243453403	20.91881686595343
28	14.80432208723107	21.362972128297297	43.85659886294546	19.97610692152617
29	17.810370022719756	19.601284252967968	41.691501957312454	20.89684376699982
30	18.033727640238503	20.54868748066687	44.272167763543855	17.145417115550767
31	19.19552858102847	19.826135187891328	41.742914742242746	19.235421488837453
32	20.179411419611522	20.538447589892375	39.44981920192851	19.83232178856759
33	19.47349894934454	19.3858198845879	38.496656035668956	22.64402513039861
34	18.63894785122292	23.10631353265565	39.34870028053034	18.906038335591088
35	17.245256050602126	23.387270535780953	37.70477114910774	21.66270226450918
36	19.751255986602807	24.421286172947486	35.02021311772675	20.80724472272296
37	19.059210035092956	23.1528197032565	36.563663320924576	21.224306940725963
38	21.626009322567228	22.522853089567043	36.07641518490469	19.774722402961036
39	20.38634254567951	22.849889601177587	34.91909419632857	21.84467365681433
40	22.520293116873418	22.322961888406525	34.602937568665936	20.55380742605412
41	18.953611161480943	23.045087519066463	35.48164819575258	22.519653123700014
42	18.864865441435292	22.521786434278035	35.50895457115124	23.104393553135434
43	20.331516463824386	22.27816236626809	33.66172094164329	23.72860022826423
44	21.45278450363196	22.46845366982752	34.341820353916226	21.73694147262429
45	19.372380027946367	21.70536847606959	34.268861132147926	24.65339036383612
46	21.210013759853226	23.43100340263037	33.203699160542286	22.155283676974115
47	17.7627971968299	22.441573956544463	39.43488602788237	20.360742818743265
48	19.572057898049085	23.80219944320594	34.78810892683811	21.837633731906863
49	18.85825217864343	22.318695267250483	36.34073236552143	22.482320188584655
50	20.04906614329447	21.311559343367005	35.252957301788776	23.38641721154974
51	19.264434512698532	21.57843649667737	34.41819287260936	24.73893611801474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	372.5
2	659.0
3	15408.5
4	30158.0
5	20182.0
6	10206.0
7	9647.0
8	9088.0
9	8917.5
10	8747.0
11	8229.0
12	7711.0
13	7433.5
14	7156.0
15	6600.5
16	6045.0
17	5536.0
18	5027.0
19	4603.5
20	4180.0
21	3940.0
22	3700.0
23	3589.0
24	3478.0
25	3676.0
26	4220.5
27	4567.0
28	4840.0
29	5113.0
30	5824.0
31	6535.0
32	7557.5
33	8580.0
34	9490.0
35	10400.0
36	11336.0
37	12272.0
38	12971.5
39	13671.0
40	14536.5
41	15402.0
42	16571.5
43	17741.0
44	19571.0
45	21401.0
46	32925.0
47	44449.0
48	39124.5
49	33800.0
50	32831.0
51	31862.0
52	29158.0
53	26454.0
54	25061.0
55	23668.0
56	22499.0
57	21330.0
58	19758.5
59	18187.0
60	16487.0
61	14787.0
62	13049.5
63	11312.0
64	9927.5
65	8543.0
66	7148.0
67	5753.0
68	4880.0
69	4007.0
70	3576.5
71	3146.0
72	2669.0
73	2192.0
74	1862.5
75	1235.0
76	937.0
77	741.0
78	545.0
79	369.5
80	194.0
81	157.0
82	120.0
83	93.0
84	66.0
85	58.0
86	50.0
87	31.5
88	13.0
89	9.0
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	468755.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.67423701010386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.34665515669876	34.21730386262538
2	7.103218315507956	6.204552804920103
3	3.305715260135848	4.331237752772705
4	2.172184747358928	3.7947404594354563
5	1.6729980530678712	3.6533456743564257
6	1.3131087205646181	3.4409412889184026
7	1.0401941258303828	3.180077935162376
8	0.8586785517607533	3.0001704468073496
9	0.7136002046195905	2.8049350020313146
>10	3.3781057846041054	21.888153791333508
>50	0.05952559425285131	1.7308991348183762
>100	0.03051311974305012	2.2919175594314996
>500	0.0020008603110196798	0.6110469367772285
>1k	0.0020008603110196798	1.7800537865072785
>5k	0.0010004301555098399	2.52895221313307
>10k+	5.002150777549199E-4	4.5416713509695406
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20789	4.43493936064682	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	6563	1.400091732354855	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	5013	1.0694285927616773	No Hit
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	3848	0.8208979104222889	No Hit
GAACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCT	1531	0.3266098494949387	No Hit
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1494	0.31871660035626287	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTCGTCCT	1275	0.2719970986976139	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTC	877	0.18709133769239794	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTA	828	0.17663811586009748	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCT	547	0.11669208861772142	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCATTCGTCCTCGTATGCCG	545	0.11626542650211731	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08831905793004875	0.0
2	0.0	0.0	0.0	0.5663939584644431	0.0
3	0.0	0.0	0.0	0.710819084596431	0.0
4	0.0	0.0	0.0	1.2659064969973655	0.0
5	0.0	0.0	0.0	2.460133758573242	0.0
6	0.0	0.0	0.0	2.775863724120276	0.0
7	0.0	0.0	0.0	3.116340092372348	0.0
8	0.0	0.0	0.0	3.5905750338663056	0.0
9	0.0	0.0	0.0	3.8004927947435228	0.0
10	0.0	0.0	0.0	5.764205181811394	0.0
11	0.0	0.0	0.0	6.282812983328178	0.0
12	0.0	0.0	0.0	7.893249138675854	0.0
13	0.0	0.0	0.0	8.102313575321864	0.0
14	0.0	0.0	0.0	8.233725506927927	0.0
15	0.0	0.0	0.0	8.613668120873378	0.0
16	0.0	0.0	0.0	8.83766573156553	0.0
17	0.0	0.0	0.0	9.060810018026475	0.0
18	0.0	0.0	0.0	9.284807628718626	0.0
19	0.0	0.0	0.0	9.793602201576517	0.0
20	0.0	0.0	0.0	10.001386651875713	0.0
21	0.0	0.0	0.0	10.253117300082133	0.0
22	0.0	0.0	0.0	10.59935360689486	0.0
23	0.0	0.0	0.0	10.854924214141716	0.0
24	0.0	0.0	0.0	11.064415312903328	0.0
25	0.0	0.0	0.0	11.255559940693965	0.0
26	0.0	0.0	0.0	11.426011455877804	0.0
27	0.0	0.0	0.0	11.617369414726243	0.0
28	0.0	0.0	0.0	11.79400753058634	0.0
29	0.0	0.0	0.0	12.004565284636964	0.0
30	0.0	0.0	0.0	12.238589455045814	0.0
31	0.0	0.0	0.0	12.422907488986784	0.0
32	0.0	0.0	0.0	12.614478778893025	0.0
33	0.0	0.0	0.0	12.810103358897505	0.0
34	0.0	0.0	0.0	13.027914368913398	0.0
35	0.0	0.0	0.0	13.283911638275859	0.0
36	0.0	0.0	0.0	13.478896225106933	0.0
37	0.0	0.0	0.0	13.679214088383057	0.0
38	0.0	0.0	0.0	13.962091071028576	0.0
39	0.0	0.0	0.0	14.440379302620771	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTACG	70	0.0	45.000004	4
CGCGCAA	25	3.887259E-5	45.0	16
TCCGTCA	20	7.029007E-4	45.0	28
TGTCGTG	20	7.029007E-4	45.0	1
CGTTTTT	9520	0.0	44.267334	1
TACGGCT	745	0.0	41.677853	7
ACCGGTG	65	0.0	41.53846	17
CGACCAC	510	0.0	41.47059	12
GAATGAT	2615	0.0	41.38623	1
TTACGGG	55	6.002665E-11	40.909092	3
ATGATAC	2480	0.0	40.64516	3
AATGATA	2490	0.0	40.391563	2
ATACGGC	1625	0.0	40.153847	6
TGATACC	910	0.0	40.054943	4
GTTTTTT	10665	0.0	40.000004	2
ACGGCTG	780	0.0	39.807693	8
GATACCT	915	0.0	39.590164	5
GCGACCA	535	0.0	39.53271	11
TCGTTGA	80	0.0	39.375	24
ATCCGGC	40	3.4523873E-7	39.375	6
>>END_MODULE