FastQCFastQC Report
Sat 14 Jan 2017
SRR2933266.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933266.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences510247
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCG134012.626375069329168No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTC125602.461552934167178No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124942.4486180222519685No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGC122332.39746632513273No Hit
GAACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCT29880.5855987394340388No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCAGCTATT25580.5013258284713089No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTC25340.49662222413850543No Hit
GCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTTCTGC24180.47388813652995515No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTA21570.4227364394107168No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCT20520.4021581704547013No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGCAGCTATTCGT9040.17716909653559942No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATGCCGTCTT8570.1679578713838592No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTAT5670.11112265236248325No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGCAGCTATTCGTATG5430.10641904802967973No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT110550.044.3080021
TACGGCT15450.042.5242737
ACGGCTG15450.042.5242738
GATACCT15200.042.18755
TGATACC15250.042.0491834
CGGCTGT16000.041.4843759
CCGATGA4250.041.29411718
AATGATC3750.040.82
CGATGAA4250.040.76470619
GAATCTG14150.040.7067151
ATACCTG15950.040.4858936
GAATGAT51500.040.325241
ATGATAC49600.040.1461683
CGACCAC12150.040.012
CACAACG451.9246727E-840.012
ATACGGC34500.039.978266
AATGATA49300.039.7515222
CACCGGT850.039.70588316
AATCTGT14600.039.6061672
GCGACCA12650.039.48616411
Produced by FastQC (version 0.11.5)