FastQCFastQC Report
Sat 14 Jan 2017
SRR2933260.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933260.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736586
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC198722.6978519819817373TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG194662.642732824137304No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180252.4471005422313215No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC165902.252282829160478No Hit
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT54030.7335192360430418TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCT42930.5828240015422503No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC41940.56938361576245No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA38200.5186088250387599No Hit
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC36300.4928141452593451TruSeq Adapter, Index 16 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT34750.4717711170182436No Hit
GAATGATACCTGTCTCTTATACACATCTGACGGTCTCTCTCTCGTATGCCG21880.2970461018808394No Hit
GAATGATACGGCTGTCTCTTATACACATCTGAGGCTCTCTCTCTCGTATGC21180.2875427988042129No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGT16330.2216984846304437No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT15350.2083938603231666No Hit
GAATCTGTCTCTTATACACATCTGATGCTCTCTCTCTCGTATGCCGTCTTC11600.15748330812695327TruSeq Adapter, Index 16 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGATCTCTCTCTCGTATGCCGTCTTC10270.13942703228136294No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATG9320.12652969239165557No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTAT9320.12652969239165557No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCGA207.0316344E-445.011
CGATGCG351.2111377E-745.010
CACCGGT1400.045.016
TAAGCGG207.0316344E-445.034
ATGCCGA302.1644373E-644.99999641
CGTTTTT155300.044.4494551
CCGATGA6950.043.38129418
CGGCTGT23100.042.2727289
TGATACC27750.041.918924
CGATGAA7250.041.8965519
GATACCT27900.041.6935465
ACGGCTG23250.041.6129048
ACCGGTG1150.041.08695617
CCACCGG1700.041.0294115
TACGGCT23600.040.995767
GCGACTG5050.040.990111
GCGACCT2200.040.90909211
TTCCGCG556.002665E-1140.9090921
GCTACGA556.002665E-1140.90909210
GCGAGAC950.040.26315721
Produced by FastQC (version 0.11.5)