FastQCFastQC Report
Sat 14 Jan 2017
SRR2933259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences461926
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254685.513437217216611No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG94372.0429679212687746No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC73491.5909474677762239No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC72471.568866008841243TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT27060.5858081164515528TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC22550.48817343037629407TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCT20230.43794893554378844No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC17010.3682408004745349No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA14880.3221295185808982No Hit
GAATGATACCTGTCTCTTATACACATCTGACGGTCTCTCTCTCGTATGCCG12160.2632456280876158No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT11200.2424630785017514No Hit
GAATGATACGGCTGTCTCTTATACACATCTGAGGCTCTCTCTCTCGTATGC8310.17989894485263874No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCTCTCTCTCGTATGCCG7800.16885821538514825No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGT6820.14764269601624502No Hit
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT6750.14612730177560906TruSeq Adapter, Index 27 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT6710.1452613622095314No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC5280.1143040227222542TruSeq Adapter, Index 16 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGG207.0288987E-445.0000042
CGATTCG406.7993824E-945.00000410
CGTTAGG207.0288987E-445.0000042
GCGGGTC207.0288987E-445.0000045
ATACGGA207.0288987E-445.00000428
ATCCGGC453.8380676E-1045.06
CCGATGA1250.044.99999618
CGTTTTT105100.044.3363461
CTTTGCG1450.043.4482731
CACCGGT1250.043.19999716
GCGACCT1200.043.12499611
GGCGACT1900.042.6315810
ACGGCTG12650.042.3320168
TACGGCT12900.042.034887
CGGTGTC750.042.00000419
TGATACC15450.041.7961164
GCGAGAC700.041.78571321
GCGATTC700.041.7857139
GAATGAT43850.041.6647641
GCGACTG2000.041.62511
Produced by FastQC (version 0.11.5)