##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933259.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	461926
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.216621277001078	31.0	26.0	31.0	16.0	33.0
2	28.281794053593	31.0	28.0	33.0	16.0	34.0
3	28.481414772063058	31.0	28.0	33.0	16.0	34.0
4	31.747087195784605	35.0	28.0	37.0	19.0	37.0
5	33.56310318102899	35.0	33.0	37.0	28.0	37.0
6	32.42380381273191	35.0	32.0	37.0	17.0	37.0
7	32.056106389335085	35.0	31.0	35.0	25.0	37.0
8	32.69801007087715	35.0	32.0	35.0	26.0	37.0
9	32.75218108528206	35.0	31.0	37.0	25.0	39.0
10	31.26781562414759	35.0	27.0	38.0	15.0	39.0
11	32.473253291652775	35.0	30.0	39.0	17.0	39.0
12	33.079757363733584	35.0	31.0	39.0	23.0	39.0
13	33.29617730978555	35.0	32.0	39.0	25.0	39.0
14	33.15750791252278	36.0	31.0	39.0	18.0	41.0
15	33.80437126292956	36.0	32.0	39.0	25.0	41.0
16	34.02292791486082	36.0	32.0	40.0	25.0	41.0
17	31.945311586704364	34.0	27.0	39.0	18.0	41.0
18	32.788173863346074	35.0	30.0	39.0	22.0	39.0
19	32.7248888350082	36.0	30.0	37.0	19.0	39.0
20	32.43095647354771	34.0	30.0	37.0	24.0	39.0
21	32.903400111706205	35.0	31.0	37.0	24.0	39.0
22	33.352770790126556	35.0	31.0	38.0	26.0	39.0
23	34.24768036438737	35.0	33.0	39.0	27.0	40.0
24	32.61796045254002	35.0	31.0	37.0	22.0	39.0
25	31.34551205171391	34.0	28.0	37.0	18.0	39.0
26	30.76681546394877	33.0	25.0	37.0	18.0	39.0
27	32.59184588007603	35.0	31.0	38.0	22.0	39.0
28	32.17280257010863	35.0	30.0	38.0	20.0	39.0
29	32.684181016006896	35.0	31.0	38.0	21.0	40.0
30	31.494533756489133	34.0	29.0	38.0	17.0	40.0
31	31.635300892350724	34.0	30.0	37.0	18.0	40.0
32	31.82611500543377	35.0	30.0	37.0	18.0	40.0
33	31.32144326147478	35.0	30.0	38.0	15.0	40.0
34	31.073702281317786	35.0	30.0	38.0	15.0	40.0
35	30.485434896498575	35.0	27.0	38.0	10.0	40.0
36	30.252964760589357	35.0	26.0	38.0	9.0	40.0
37	30.636426613786625	35.0	29.0	38.0	9.0	40.0
38	30.483322003957344	35.0	27.0	38.0	9.0	40.0
39	30.115074708936064	35.0	26.0	38.0	8.0	40.0
40	29.79115053060447	34.0	24.0	38.0	8.0	40.0
41	29.210505578815653	34.0	23.0	38.0	8.0	40.0
42	29.84010642397267	35.0	25.0	38.0	8.0	40.0
43	29.855970436823213	35.0	24.0	38.0	8.0	40.0
44	29.93698774262544	35.0	25.0	38.0	8.0	40.0
45	30.241006135181827	35.0	26.0	38.0	8.0	40.0
46	29.943640323341835	35.0	24.0	38.0	7.0	40.0
47	30.10823378636405	35.0	25.0	38.0	7.0	40.0
48	29.872213298233916	35.0	25.0	38.0	7.0	40.0
49	29.804836705446327	35.0	26.0	38.0	7.0	40.0
50	30.044719284041168	35.0	26.0	38.0	7.0	40.0
51	28.155503262427317	33.0	22.0	37.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	4.0
10	14.0
11	15.0
12	30.0
13	47.0
14	57.0
15	153.0
16	371.0
17	728.0
18	1311.0
19	2255.0
20	3284.0
21	4835.0
22	6803.0
23	9205.0
24	12785.0
25	17576.0
26	22402.0
27	24472.0
28	25167.0
29	26829.0
30	30134.0
31	34538.0
32	39347.0
33	43221.0
34	46671.0
35	48634.0
36	36434.0
37	20148.0
38	4380.0
39	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.693509349982463	2.503864255313622	37.17067235877608	34.63195403592783
2	34.20201504137027	14.97837315933721	36.8621813883609	13.95743041093162
3	11.954945164376978	14.925767330697989	59.05123331442699	14.068054190498044
4	11.16239397652438	2.848724687504059	69.39682979524859	16.592051540722974
5	20.503933530478907	3.5178794871905885	58.66156050969202	17.316626472638475
6	14.233015677835844	12.264085589466712	61.19032052753038	12.312578205167062
7	55.67103821824275	1.042158267774492	40.17245186458437	3.1143516493983885
8	56.32352368128228	9.291531544013543	30.118244047747904	4.266700726956266
9	52.292358516299146	2.7218645410736784	29.9409429215935	15.044834021033674
10	29.93488134463096	22.34881777600741	35.340725570762416	12.375575308599213
11	20.1917623169079	17.596324952481567	47.06727917458641	15.144633556024125
12	17.239557851257562	16.198482007940665	47.836882963937946	18.725077176863827
13	16.883440204708116	17.567532461909483	52.94982313184363	12.599204201538775
14	14.72097262332062	21.291722050718082	45.68004398972996	18.307261336231345
15	11.10610790472933	17.839221000766354	54.7698116148474	16.284859479656916
16	13.135004308049341	18.58890818009811	45.40315981347662	22.872927698375932
17	13.925823616769787	18.63848322025606	49.44146897987989	17.99422418309426
18	13.513636383316808	17.676640847235273	48.980572645835046	19.829150123612873
19	13.684226477834113	20.557188813792685	45.62527331217554	20.13331139619766
20	15.239886908292672	20.24501760022168	50.8505691387798	13.664526352705844
21	15.827426903876379	22.93029619462858	46.33577672614228	14.906500175352763
22	13.704143087853899	17.12287249472859	48.447586842914234	20.725397574503276
23	14.114165472391683	22.702337603858627	46.9557894554539	16.227707468295787
24	15.025566865688445	18.538900170157124	46.43341141221754	20.00212155193689
25	12.475591328481185	25.28327048055316	44.01917190199296	18.221966288972695
26	13.544160753021048	19.06820572992211	48.0113697865026	19.376263730554243
27	14.748033234760547	20.40954611777644	44.86714322207453	19.975277425388484
28	11.234266960508826	19.768534353987434	50.571953083394305	18.425245602109428
29	14.804319306555596	16.48510800431238	48.29734632819975	20.41322636093227
30	13.158601161224958	20.756571398882073	47.81783229348424	18.266995146408732
31	14.958023579534386	18.094023718084713	46.219307854504834	20.728644847876065
32	15.818551023324082	21.24322943501773	44.045366573866815	18.892852967791377
33	14.15269978308214	18.491489978914373	43.62993206704104	23.725878170962446
34	15.18013707823331	19.943670631226645	44.74807653173885	20.128115758801194
35	12.676056338028168	20.542467841169366	42.3074258647489	24.474049956053566
36	14.447768690223109	20.018141433909328	43.6316639461732	21.902425929694367
37	14.038395760359885	21.487857362434674	40.573598368569854	23.90014850863558
38	15.40398245606439	21.232405190441757	43.18289076605344	20.180721587440413
39	16.468222182773864	21.414252499318074	38.40182193684703	23.715703381061033
40	15.895403159813476	20.76176703627854	44.62208232487455	18.720747479033438
41	15.126665310028011	22.870979334352256	37.130189684061946	24.872165671557784
42	15.523049146400073	21.068959097344596	43.55069859674494	19.857293159510398
43	17.518823361317615	20.45847170325983	37.93529699562267	24.08740793979988
44	17.715175157925728	21.204695124327273	37.62507414607534	23.455055571671654
45	15.72611197464529	20.516489654187033	39.12791226300317	24.62948610816451
46	19.674796395959525	22.869896909894656	36.4820339188528	20.97327277529301
47	13.745708187025626	20.276840879275035	45.17152097955084	20.8059299541485
48	16.77259994025017	22.122158094586577	37.07087282378563	24.034369141377624
49	15.519585388135763	19.832830366768704	42.757064984434734	21.8905192606608
50	16.643358460013076	19.363924091737637	39.65981564146638	24.332901806782907
51	16.387689803128637	19.533864731580383	37.77033550828487	26.3081099570061
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	292.0
2	522.0
3	18022.0
4	35522.0
5	23662.5
6	11803.0
7	11158.0
8	10513.0
9	10283.5
10	10054.0
11	9585.0
12	9116.0
13	8551.5
14	7987.0
15	7319.5
16	6652.0
17	6189.0
18	5726.0
19	5279.0
20	4832.0
21	4623.0
22	4414.0
23	4332.5
24	4251.0
25	4560.0
26	5230.0
27	5591.0
28	6360.5
29	7130.0
30	7681.5
31	8233.0
32	9207.0
33	10181.0
34	11559.5
35	12938.0
36	13406.5
37	13875.0
38	14629.5
39	15384.0
40	16942.5
41	18501.0
42	19777.0
43	21053.0
44	22980.5
45	24908.0
46	44325.0
47	63742.0
48	52012.5
49	40283.0
50	36072.5
51	31862.0
52	26235.5
53	20609.0
54	17944.0
55	15279.0
56	13076.0
57	10873.0
58	9710.0
59	8547.0
60	7206.5
61	5866.0
62	4895.5
63	3925.0
64	3314.5
65	2704.0
66	2177.5
67	1651.0
68	1287.5
69	924.0
70	762.5
71	601.0
72	433.5
73	266.0
74	258.0
75	232.5
76	215.0
77	169.0
78	123.0
79	80.0
80	37.0
81	29.0
82	21.0
83	23.5
84	26.0
85	14.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	461926.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.06448263099178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4133035348174	36.68848403076441
2	7.074727513762722	6.376378703259197
3	2.841646819991448	3.841720312887529
4	1.786624932049404	3.2205331287374896
5	1.2781687304019396	2.880000627533751
6	0.9669764780279816	2.614577681920175
7	0.7550806631904572	2.3819123601940877
8	0.6401765292612398	2.3079379266929396
9	0.537053001939067	2.1781814110024573
>10	2.6085193176489594	18.06378822474032
>50	0.052053179720581606	1.616132964783392
>100	0.03732114772418703	3.1855722344317714
>500	0.002946406399277923	0.9221451546284956
>1k	0.0034374741324909104	2.768205840952208
>5k	0.0014732031996389616	5.318434005564346
>10k+	4.910677332129873E-4	5.6359953919074925
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25468	5.513437217216611	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCG	9437	2.0429679212687746	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGC	7349	1.5909474677762239	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTC	7247	1.568866008841243	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCT	2706	0.5858081164515528	TruSeq Adapter, Index 27 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	2255	0.48817343037629407	TruSeq Adapter, Index 16 (95% over 24bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTCTCTCT	2023	0.43794893554378844	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTC	1701	0.3682408004745349	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTA	1488	0.3221295185808982	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGGTCTCTCTCTCGTATGCCG	1216	0.2632456280876158	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCT	1120	0.2424630785017514	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGAGGCTCTCTCTCTCGTATGC	831	0.17989894485263874	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTCTCTCTCTCGTATGCCG	780	0.16885821538514825	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGT	682	0.14764269601624502	No Hit
CTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGCT	675	0.14612730177560906	TruSeq Adapter, Index 27 (95% over 24bp)
GAATGCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTT	671	0.1452613622095314	No Hit
CCTGTCTCTTATACACATCTGACGCTCTCTCTCTCGTATGCCGTCTTCTGC	528	0.1143040227222542	TruSeq Adapter, Index 16 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1648489151942086E-4	0.0	0.0	0.1835791880084689	0.0
2	2.1648489151942086E-4	0.0	0.0	1.0122833527448118	0.0
3	2.1648489151942086E-4	0.0	0.0	1.2822400124695297	0.0
4	2.1648489151942086E-4	0.0	0.0	2.367262288764867	0.0
5	2.1648489151942086E-4	0.0	0.0	4.853807752756935	0.0
6	2.1648489151942086E-4	0.0	0.0	5.386144101003191	0.0
7	2.1648489151942086E-4	0.0	0.0	6.001827132484424	0.0
8	2.1648489151942086E-4	0.0	0.0	6.889415187714049	0.0
9	2.1648489151942086E-4	0.0	0.0	7.151794876235588	0.0
10	2.1648489151942086E-4	0.0	0.0	10.341266782991214	0.0
11	2.1648489151942086E-4	0.0	0.0	11.227122959088685	0.0
12	2.1648489151942086E-4	0.0	0.0	14.049220004935856	0.0
13	2.1648489151942086E-4	0.0	0.0	14.446469780873993	0.0
14	2.1648489151942086E-4	0.0	0.0	14.678541584582812	0.0
15	2.1648489151942086E-4	0.0	0.0	15.347046929594784	0.0
16	2.1648489151942086E-4	0.0	0.0	15.765512224901824	0.0
17	2.1648489151942086E-4	0.0	0.0	16.15475205985374	0.0
18	2.1648489151942086E-4	0.0	0.0	16.571485476028627	0.0
19	2.1648489151942086E-4	0.0	0.0	17.540038880686517	0.0
20	2.1648489151942086E-4	0.0	0.0	17.953308538597092	0.0
21	2.1648489151942086E-4	0.0	0.0	18.400566324476216	0.0
22	2.1648489151942086E-4	0.0	0.0	19.06322657741716	0.0
23	2.1648489151942086E-4	0.0	0.0	19.544256006373317	0.0
24	2.1648489151942086E-4	0.0	0.0	19.930032083060922	0.0
25	2.1648489151942086E-4	0.0	0.0	20.239388993042176	0.0
26	4.3296978303884173E-4	0.0	0.0	20.549395357697986	0.0
27	4.3296978303884173E-4	0.0	0.0	20.87801942302447	0.0
28	4.3296978303884173E-4	0.0	0.0	21.161398146023387	0.0
29	4.3296978303884173E-4	0.0	0.0	21.47508475383503	0.0
30	4.3296978303884173E-4	0.0	0.0	21.843542039201083	0.0
31	4.3296978303884173E-4	0.0	0.0	22.150084645592585	0.0
32	4.3296978303884173E-4	0.0	0.0	22.452514039045216	0.0
33	4.3296978303884173E-4	0.0	0.0	22.759922585002794	0.0
34	4.3296978303884173E-4	0.0	0.0	23.065382766936697	0.0
35	4.3296978303884173E-4	0.0	0.0	23.408727804886496	0.0
36	4.3296978303884173E-4	0.0	0.0	23.722847382481177	0.0
37	4.3296978303884173E-4	0.0	0.0	24.03566805072674	0.0
38	4.3296978303884173E-4	0.0	0.0	24.369920723232724	0.0
39	4.3296978303884173E-4	0.0	0.0	24.797261899092064	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCGG	20	7.0288987E-4	45.000004	2
CGATTCG	40	6.7993824E-9	45.000004	10
CGTTAGG	20	7.0288987E-4	45.000004	2
GCGGGTC	20	7.0288987E-4	45.000004	5
ATACGGA	20	7.0288987E-4	45.000004	28
ATCCGGC	45	3.8380676E-10	45.0	6
CCGATGA	125	0.0	44.999996	18
CGTTTTT	10510	0.0	44.336346	1
CTTTGCG	145	0.0	43.448273	1
CACCGGT	125	0.0	43.199997	16
GCGACCT	120	0.0	43.124996	11
GGCGACT	190	0.0	42.63158	10
ACGGCTG	1265	0.0	42.332016	8
TACGGCT	1290	0.0	42.03488	7
CGGTGTC	75	0.0	42.000004	19
TGATACC	1545	0.0	41.796116	4
GCGAGAC	70	0.0	41.785713	21
GCGATTC	70	0.0	41.785713	9
GAATGAT	4385	0.0	41.664764	1
GCGACTG	200	0.0	41.625	11
>>END_MODULE