SRR2933258.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2933258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39085
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1369	3.5026224894460793	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	1000	2.558526288857618	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	795	2.0340283996418065	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	375	0.9594473583216069	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	303	0.7752334655238583	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGCGTT	217	0.5552002046821031	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTA	128	0.3274913649737751	No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111	0.2839964180631956	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCT	92	0.23538441857490086	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTC	84	0.21491620826403993	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGT	72	0.1842138927977485	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATG	58	0.14839452475374185	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	58	0.14839452475374185	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.13560189330945374	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.13560189330945374	No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	53	0.13560189330945374	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	50	0.1279263144428809	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTT	49	0.12536778815402327	No Hit
GTATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	48	0.12280926186516566	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.1151336829985928	No Hit
GGATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	44	0.11257515670973518	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCT	44	0.11257515670973518	No Hit
GATTCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	44	0.11257515670973518	No Hit
GGATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	42	0.10745810413201996	No Hit
GTATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	40	0.10234105155430473	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	20	6.949808E-4	45.000004	14
AATCCTA	20	6.949808E-4	45.000004	13
AACTGTC	40	6.528353E-9	45.000004	2
GATACCT	160	0.0	45.000004	5
TCCTAGC	20	6.949808E-4	45.000004	15
ATGATGC	20	6.949808E-4	45.000004	3
CTGGGAT	30	2.1119304E-6	45.000004	5
CGCTTTT	105	0.0	45.000004	1
ATGAATG	40	6.528353E-9	45.000004	21
CTTGGGA	35	1.1721204E-7	45.000004	4
TTGGGCT	20	6.949808E-4	45.000004	5
TTGGGCA	20	6.949808E-4	45.000004	5
GAACTGT	40	6.528353E-9	45.000004	1
CTAATCC	20	6.949808E-4	45.000004	11
TAATCCT	20	6.949808E-4	45.000004	12
GCATGGG	20	6.949808E-4	45.000004	3
TTGATAC	20	6.949808E-4	45.000004	3
TTCGGGC	20	6.949808E-4	45.000004	4
GACTAAT	20	6.949808E-4	45.000004	9
CGGGGCT	25	3.8217833E-5	45.0	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content