##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933258.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39085
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.554483817321223	30.0	25.0	31.0	16.0	33.0
2	24.197518229499806	28.0	16.0	31.0	10.0	33.0
3	24.439247793271075	28.0	16.0	31.0	10.0	34.0
4	30.433977229116028	32.0	28.0	35.0	19.0	37.0
5	33.14895740053729	35.0	32.0	35.0	28.0	37.0
6	32.799692976845336	35.0	32.0	35.0	28.0	37.0
7	32.70016630420878	35.0	32.0	35.0	27.0	37.0
8	30.832390942816936	35.0	29.0	35.0	17.0	37.0
9	31.68537802225918	35.0	30.0	38.0	16.0	39.0
10	32.903031853652294	35.0	31.0	39.0	22.0	39.0
11	33.91822949980811	37.0	32.0	39.0	27.0	39.0
12	34.22448509658437	37.0	32.0	39.0	26.0	39.0
13	34.44024561852373	37.0	32.0	39.0	27.0	39.0
14	35.16001023410516	38.0	33.0	40.0	25.0	41.0
15	35.72521427657669	38.0	33.0	40.0	27.0	41.0
16	35.325342202891136	38.0	33.0	40.0	27.0	41.0
17	35.11224254829219	37.0	33.0	40.0	27.0	41.0
18	34.49036714852245	37.0	33.0	39.0	25.0	40.0
19	33.70981194831777	36.0	32.0	37.0	25.0	40.0
20	33.295688883203276	35.0	32.0	37.0	25.0	39.0
21	33.45104259946271	35.0	32.0	37.0	26.0	39.0
22	33.933990021747476	35.0	33.0	38.0	27.0	39.0
23	34.375156709735194	35.0	33.0	38.0	28.0	40.0
24	33.29085326851733	35.0	33.0	37.0	25.0	39.0
25	32.41176922092875	35.0	31.0	37.0	21.0	39.0
26	32.230062683894076	34.0	30.0	37.0	22.0	39.0
27	33.22348727133171	35.0	32.0	37.0	25.0	39.0
28	33.262965331968786	35.0	33.0	37.0	24.0	40.0
29	32.99800434949469	35.0	32.0	38.0	23.0	40.0
30	32.20506588205194	35.0	31.0	37.0	21.0	40.0
31	32.02008443136753	35.0	31.0	37.0	18.0	40.0
32	31.157528463604965	35.0	30.0	37.0	15.0	40.0
33	30.532045541767943	35.0	27.0	38.0	10.0	40.0
34	29.967557886657286	35.0	24.0	38.0	8.0	40.0
35	29.287936548548036	35.0	22.0	38.0	7.0	40.0
36	28.650633235256493	35.0	18.0	38.0	7.0	40.0
37	28.533606242804144	35.0	18.0	38.0	7.0	40.0
38	28.363489829858	34.0	18.0	38.0	7.0	40.0
39	27.870538569783804	34.0	17.0	38.0	7.0	40.0
40	27.640194447997953	33.0	16.0	38.0	7.0	40.0
41	26.89886145580146	33.0	15.0	37.0	7.0	40.0
42	26.781757707560445	33.0	15.0	37.0	7.0	40.0
43	26.960368427785596	33.0	15.0	37.0	7.0	40.0
44	27.24364845848791	33.0	15.0	38.0	7.0	40.0
45	27.43720097223999	34.0	15.0	38.0	7.0	40.0
46	27.291237047460662	33.0	15.0	38.0	7.0	40.0
47	27.229909172316745	33.0	15.0	38.0	7.0	40.0
48	27.27870026864526	33.0	15.0	37.0	7.0	40.0
49	27.329487015479085	34.0	15.0	38.0	7.0	40.0
50	27.376435972879623	33.0	15.0	38.0	7.0	40.0
51	26.822873225022388	33.0	15.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	6.0
13	18.0
14	11.0
15	35.0
16	44.0
17	89.0
18	146.0
19	218.0
20	383.0
21	520.0
22	821.0
23	1153.0
24	1543.0
25	2067.0
26	2260.0
27	2382.0
28	2161.0
29	2103.0
30	2197.0
31	2538.0
32	2786.0
33	3302.0
34	3590.0
35	3683.0
36	2659.0
37	1630.0
38	641.0
39	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.011001663042087	2.144045030062684	29.325828322886018	46.51912498400921
2	39.549699373161054	13.56786490981195	31.973903031853652	14.90853268517334
3	12.124856082896251	14.463349110912116	56.13918383011386	17.27261097607778
4	8.67852117180504	2.5943456569016248	73.30689522834847	15.420237942944864
5	20.058846104643724	2.6020212357681975	63.37981322758092	13.959319432007163
6	12.073685557119099	13.342714596392478	65.01982857873865	9.563771267749777
7	44.019444799795316	1.051554304720481	52.73378533964437	2.1952155558398365
8	40.660099782525265	10.47204810029423	46.009978252526544	2.857873864653959
9	38.42138927977485	2.013560189330945	44.68210310860944	14.882947422284765
10	29.43072790072918	11.963668926698222	47.25853908148906	11.347064091083537
11	24.211334271459638	11.65408724574645	53.358065754125626	10.776512728668287
12	19.07381348343354	10.692081361135985	53.90047332736344	16.333631828067034
13	16.899066137904565	10.656261993091979	61.82678777024434	10.617884098759115
14	14.468466163489829	14.818984265063323	56.238966355379304	14.473583216067546
15	11.469873352948703	10.843034412178586	64.00153511577331	13.685557119099398
16	14.609185109376998	12.247665344761417	53.73161059229884	19.411538953562747
17	14.944352053217347	13.248049123704748	58.917743379813224	12.88985544326468
18	14.949469105795062	12.173468082384547	56.77881540232826	16.098247409492135
19	14.893181527440195	15.653063835230906	53.96443648458488	15.48931815274402
20	15.141358577459382	15.184853524369963	59.263144428809014	10.410643469361649
21	14.923883842906486	19.329666112319305	54.808750159907895	10.937699884866317
22	13.122681335550723	11.129589356530637	59.227325060765004	16.52040424715364
23	13.744403223743124	18.252526544710246	56.22617372393501	11.776896507611616
24	13.979787642318026	14.517078162978125	54.10515543047205	17.3979787642318
25	14.302161954714085	20.65754125623641	51.49290008954842	13.547396699501087
26	12.654471024689778	12.72866828706665	56.96047076883715	17.656389919406422
27	14.343098375335806	14.3738006908021	53.96443648458488	17.318664449277215
28	11.474990405526416	15.389535627478573	58.058078546757066	15.077395420237943
29	16.22873225022387	13.624152488166816	54.13074069336062	16.01637456824869
30	14.788281949597032	17.011641294614304	55.908916464116665	12.291160291671996
31	17.03466803121402	15.03645899961622	50.97096072662146	16.957912242548293
32	19.59319432007164	17.832928233337597	47.957016758347194	14.616860688243571
33	17.965971600358195	15.686324676986056	45.27568120762441	21.072022515031342
34	18.6900345401049	18.802609696814635	47.44019444799795	15.067161315082512
35	17.05769476781374	21.138544198541638	44.505564794678264	17.298196238966355
36	19.084047588588973	22.978124600230267	42.07752334655239	15.860304464628374
37	18.203914545221952	21.38672124856083	41.701419982090314	18.7079442241269
38	23.97339132659588	23.62031469873353	37.541256236407825	14.86503773826276
39	19.621338109249074	19.75182294998081	37.69220928745043	22.93462965331969
40	27.01292055775873	19.810669054624537	37.99411538953563	15.182294998081106
41	19.682742740181656	21.220417039785083	39.53434821542791	19.562492004605346
42	20.498912626327233	21.739797876423182	40.39401304848408	17.367276448765512
43	19.79531789689139	19.148010745810414	41.3585774593834	19.698093897914802
44	22.832288601765384	20.424715363950366	37.909684022003326	18.833312012280924
45	18.434181911219138	20.703594729435846	38.40092106946399	22.461302289881026
46	20.713828834591276	21.455801458359986	36.93488550594857	20.895484201100167
47	17.50031981578611	19.298963796853013	44.873992580273764	18.326723807087117
48	19.275937060253295	23.12651912498401	37.3800690802098	20.217474734552898
49	18.36510170141998	20.654982729947548	39.85160547524626	21.12831009338621
50	18.444416016374568	19.10963285147755	40.007675578866575	22.438275553281308
51	18.797492644236918	20.457976205705513	38.17321222975566	22.571318920301906
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	345.0
2	684.0
3	1903.0
4	3122.0
5	2436.5
6	1751.0
7	1551.0
8	1351.0
9	1286.5
10	1222.0
11	1198.5
12	1175.0
13	1105.5
14	1036.0
15	966.0
16	896.0
17	824.0
18	752.0
19	674.0
20	596.0
21	556.5
22	517.0
23	522.5
24	528.0
25	507.5
26	511.5
27	536.0
28	546.5
29	557.0
30	591.0
31	625.0
32	628.0
33	631.0
34	623.0
35	615.0
36	681.5
37	748.0
38	730.5
39	713.0
40	748.5
41	784.0
42	820.5
43	857.0
44	965.5
45	1074.0
46	2702.5
47	4331.0
48	3317.0
49	2303.0
50	1950.0
51	1597.0
52	1493.0
53	1389.0
54	1384.5
55	1380.0
56	1398.0
57	1416.0
58	1365.0
59	1314.0
60	1171.0
61	1028.0
62	913.5
63	799.0
64	729.0
65	659.0
66	576.0
67	493.0
68	431.0
69	369.0
70	314.5
71	260.0
72	218.5
73	177.0
74	155.0
75	111.0
76	89.0
77	68.0
78	47.0
79	34.5
80	22.0
81	15.5
82	9.0
83	6.0
84	3.0
85	3.0
86	3.0
87	1.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	39085.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.14250991428938
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.4357933337699	74.57592426762186
2	2.8943749590727523	4.523474478700269
3	0.6351908846833868	1.4890623001151337
4	0.3274179817955602	1.0234105155430473
5	0.1571606312618689	0.6140463093258284
6	0.08512867526684566	0.3991301010617884
7	0.07858031563093446	0.42983241652807985
8	0.04256433763342283	0.26608673404119226
9	0.03929015781546723	0.27632083919662276
>10	0.25211184598258135	4.068056799283613
>50	0.02619343854364482	1.3381092490725344
>100	0.01637089908977801	2.901368811564539
>500	0.006548359635911205	4.592554688499424
>1k	0.0032741798179556024	3.5026224894460793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1369	3.5026224894460793	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	1000	2.558526288857618	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	795	2.0340283996418065	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	375	0.9594473583216069	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	303	0.7752334655238583	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGCGTT	217	0.5552002046821031	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTA	128	0.3274913649737751	No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111	0.2839964180631956	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCT	92	0.23538441857490086	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTC	84	0.21491620826403993	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGT	72	0.1842138927977485	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATG	58	0.14839452475374185	No Hit
GATTGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	58	0.14839452475374185	No Hit
CCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.13560189330945374	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.13560189330945374	No Hit
GATTGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	53	0.13560189330945374	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	50	0.1279263144428809	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTT	49	0.12536778815402327	No Hit
GTATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	48	0.12280926186516566	No Hit
CTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	45	0.1151336829985928	No Hit
GGATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	44	0.11257515670973518	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCT	44	0.11257515670973518	No Hit
GATTCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	44	0.11257515670973518	No Hit
GGATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	42	0.10745810413201996	No Hit
GTATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	40	0.10234105155430473	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.01279263144428809	0.0
2	0.0	0.0	0.0	0.17653831393117564	0.0
3	0.0	0.0	0.0	0.22259178713061276	0.0
4	0.0	0.0	0.0	0.608929256748113	0.0
5	0.0	0.0	0.0	2.2668542919278494	0.0
6	0.0	0.0	0.0	2.5022387105027506	0.0
7	0.0	0.0	0.0	2.765766918255085	0.0
8	0.0	0.0	0.0	3.1904822822054495	0.0
9	0.0	0.0	0.0	3.3670205961366255	0.0
10	0.0	0.0	0.0	6.959191505692721	0.0
11	0.0	0.0	0.0	7.107586030446463	0.0
12	0.0	0.0	0.0	9.947550211078418	0.0
13	0.0	0.0	0.0	10.21363694511961	0.0
14	0.0	0.0	0.0	10.400409364206217	0.0
15	0.0	0.0	0.0	10.886529359089165	0.0
16	0.0	0.0	0.0	11.175642829730075	0.0
17	0.0	0.0	0.0	11.308686196750672	0.0
18	0.0	0.0	0.0	11.377766406549828	0.0
19	0.0	0.0	0.0	12.188819240117692	0.0
20	0.0	0.0	0.0	12.270692081361137	0.0
21	0.0	0.0	0.0	12.332096712293719	0.0
22	0.0	0.0	0.0	12.419086606114877	0.0
23	0.0	0.0	0.0	12.50351797364718	0.0
24	0.0	0.0	0.0	12.587949341179481	0.0
25	0.0	0.0	0.0	12.646795445823205	0.0
26	0.0	0.0	0.0	12.751695023666368	0.0
27	0.0	0.0	0.0	12.86938723295382	0.0
28	0.0	0.0	0.0	12.961494179352693	0.0
29	0.0	0.0	0.0	13.048484073173851	0.0
30	0.0	0.0	0.0	13.127798388128438	0.0
31	0.0	0.0	0.0	13.209671229371882	0.0
32	0.0	0.0	0.0	13.301778175770757	0.0
33	0.0	0.0	0.0	13.365741332992197	0.0
34	0.0	0.0	0.0	13.509018805168223	0.0
35	0.0	0.0	0.0	13.624152488166816	0.0
36	0.0	0.0	0.0	13.752078802609697	0.0
37	0.0	0.0	0.0	13.864653959319432	0.0
38	0.0	0.0	0.0	14.010489957784316	0.0
39	0.0	0.0	0.0	14.35077395420238	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTAG	20	6.949808E-4	45.000004	14
AATCCTA	20	6.949808E-4	45.000004	13
AACTGTC	40	6.528353E-9	45.000004	2
GATACCT	160	0.0	45.000004	5
TCCTAGC	20	6.949808E-4	45.000004	15
ATGATGC	20	6.949808E-4	45.000004	3
CTGGGAT	30	2.1119304E-6	45.000004	5
CGCTTTT	105	0.0	45.000004	1
ATGAATG	40	6.528353E-9	45.000004	21
CTTGGGA	35	1.1721204E-7	45.000004	4
TTGGGCT	20	6.949808E-4	45.000004	5
TTGGGCA	20	6.949808E-4	45.000004	5
GAACTGT	40	6.528353E-9	45.000004	1
CTAATCC	20	6.949808E-4	45.000004	11
TAATCCT	20	6.949808E-4	45.000004	12
GCATGGG	20	6.949808E-4	45.000004	3
TTGATAC	20	6.949808E-4	45.000004	3
TTCGGGC	20	6.949808E-4	45.000004	4
GACTAAT	20	6.949808E-4	45.000004	9
CGGGGCT	25	3.8217833E-5	45.0	6
>>END_MODULE