##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933257.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	93477
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.24781497052751	31.0	28.0	33.0	25.0	34.0
2	29.494260620259528	31.0	30.0	33.0	16.0	34.0
3	29.392032264621243	31.0	30.0	34.0	16.0	34.0
4	32.57062165024551	35.0	32.0	37.0	19.0	37.0
5	34.107930293013254	35.0	33.0	37.0	30.0	37.0
6	33.35605550028349	35.0	35.0	37.0	26.0	37.0
7	32.67703285300127	35.0	32.0	37.0	26.0	37.0
8	33.55967778170031	35.0	35.0	37.0	28.0	37.0
9	34.028958995260865	35.0	32.0	39.0	27.0	39.0
10	31.956310108368903	37.0	27.0	39.0	15.0	39.0
11	32.65869679172417	37.0	30.0	39.0	17.0	39.0
12	33.45947131379911	37.0	31.0	39.0	23.0	39.0
13	33.55238186933684	37.0	32.0	39.0	23.0	39.0
14	34.463033687431135	37.0	32.0	40.0	22.0	41.0
15	34.70648394792302	38.0	32.0	40.0	25.0	41.0
16	35.02520406089199	38.0	32.0	40.0	25.0	41.0
17	33.663457321052235	36.0	31.0	40.0	21.0	41.0
18	33.83754292499759	37.0	32.0	39.0	24.0	39.0
19	33.413149758764185	37.0	32.0	37.0	24.0	39.0
20	32.947783946853235	35.0	32.0	36.0	25.0	39.0
21	33.1254961113429	35.0	32.0	37.0	25.0	39.0
22	33.07819035698621	35.0	30.0	37.0	25.0	39.0
23	34.24238047862041	35.0	33.0	38.0	28.0	40.0
24	32.58613348738192	35.0	31.0	37.0	23.0	39.0
25	32.350310771633666	35.0	31.0	37.0	21.0	39.0
26	30.832718208757235	33.0	25.0	37.0	20.0	39.0
27	32.572279812146306	34.0	31.0	37.0	23.0	40.0
28	32.95663104293035	35.0	32.0	37.0	23.0	40.0
29	32.80859462755544	35.0	31.0	38.0	22.0	40.0
30	31.809247194497043	35.0	30.0	37.0	20.0	40.0
31	32.123495619243236	35.0	30.0	37.0	21.0	40.0
32	31.665971308450207	35.0	30.0	37.0	18.0	40.0
33	31.49722391604352	35.0	30.0	38.0	15.0	40.0
34	30.782106828417685	35.0	29.0	38.0	10.0	40.0
35	30.21076842431828	35.0	27.0	38.0	8.0	40.0
36	29.463921606384456	35.0	23.0	38.0	7.0	40.0
37	29.42436107277726	35.0	23.0	38.0	7.0	40.0
38	29.305091091926357	35.0	23.0	38.0	7.0	40.0
39	28.704226708174204	34.0	22.0	37.0	7.0	40.0
40	28.324208093969638	33.0	21.0	37.0	7.0	40.0
41	27.616023192870973	33.0	18.0	37.0	7.0	40.0
42	27.94974164767804	33.0	20.0	37.0	7.0	40.0
43	28.032992072916333	33.0	20.0	37.0	7.0	40.0
44	28.07140793992105	33.0	20.0	37.0	7.0	40.0
45	28.52936016346267	34.0	20.0	37.0	7.0	40.0
46	28.32510671074168	34.0	20.0	37.0	7.0	40.0
47	28.42344106036779	34.0	20.0	37.0	7.0	40.0
48	28.406228270055735	34.0	21.0	37.0	7.0	40.0
49	28.49781229607283	34.0	20.0	37.0	7.0	40.0
50	28.635407640382127	34.0	22.0	37.0	7.0	40.0
51	27.309979995079004	32.0	20.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	6.0
12	3.0
13	15.0
14	24.0
15	26.0
16	51.0
17	131.0
18	184.0
19	290.0
20	500.0
21	764.0
22	1163.0
23	1786.0
24	2769.0
25	4087.0
26	5443.0
27	5788.0
28	5846.0
29	5594.0
30	5956.0
31	6546.0
32	7351.0
33	8193.0
34	8869.0
35	9934.0
36	6651.0
37	4246.0
38	1236.0
39	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.4967746076575	2.3171475336178955	34.1420884281695	44.04398943055511
2	45.08595697337313	10.45069910245301	31.782149619692547	12.681194304481316
3	11.73336756635322	10.49562994105502	63.747231939407556	14.023770553184207
4	10.704237405992918	2.485103287439691	71.41328883040748	15.39737047615991
5	17.592562876429497	2.910876472287301	63.93658333065888	15.559977320624323
6	13.683579918054708	9.66761877253228	65.63646672443488	11.012334584978124
7	49.24740845341635	1.1863880954673343	47.150635985322594	2.4155674657937247
8	50.80394107641452	7.254190870481509	39.226761663296855	2.715106389807118
9	47.167752495266214	2.3246360067182303	39.47602083935086	11.0315906586647
10	30.186035067449744	13.22464349519133	44.61846229553794	11.970859141820982
11	23.62185350407052	13.387250339655743	50.86063951560277	12.130256640670966
12	19.21007306610182	11.734437348224697	52.92424874568076	16.131240839992724
13	17.119719289236922	12.113140130727345	58.78772318324293	11.979417396792794
14	14.740524407073396	14.09972506605903	55.56874953197043	15.591000994897142
15	12.490773131358516	12.190164425473645	60.256533692780046	15.062528750387797
16	15.062528750387797	12.358120179295442	52.86862008836398	19.71073098195278
17	15.98147137798603	13.351947537897022	55.652192517945586	15.014388566171357
18	15.896958610139391	12.515378114402473	54.570643045882946	17.017020229575188
19	15.794259550477657	15.611326850455193	51.505717984103036	17.08869561496411
20	16.22003273532527	16.243567936497747	55.062742706762094	12.473656621414895
21	16.45752431079303	18.023684970634488	52.47066123217476	13.048129486397725
22	14.876386704750901	12.841661585202777	55.184697840110395	17.09725386993592
23	14.718058987772393	17.71665757352076	53.30830043754079	14.256983001166063
24	14.913829070252577	15.246531232281738	52.23637900232143	17.60326069514426
25	15.746119366261219	18.908394578345476	49.860393465772326	15.485092589620978
26	14.325449040940551	14.489125667276442	53.37890604105823	17.80651925072478
27	14.808990446847888	14.93308514393915	52.3369384982402	17.920985910972753
28	13.15938680103127	15.756817184975983	54.65301624998663	16.43077976400612
29	16.720690651176227	14.660290766712667	51.84911796484697	16.76990061726414
30	15.693700054558876	16.92715855237117	53.238764615894816	14.140376777175135
31	16.911111824299024	16.027471998459514	49.963092525434064	17.098323651807394
32	19.125560298255188	17.410699958278506	47.825668346224205	15.638071397242104
33	18.03759213496368	16.116263893792055	45.83801362902104	20.00813034222322
34	18.253688073001914	18.68374038533543	46.88105095371054	16.181520587952118
35	16.877948586283257	21.02335333825433	44.641997496710424	17.45670057875199
36	18.97472105437701	22.223648598050858	42.55378328358847	16.247847063983652
37	18.184152251355947	21.568942092707296	41.669073675877485	18.577831980059266
38	22.809889063619927	21.92303989216599	39.13903954983579	16.1280314943783
39	19.813430041614517	19.72891727376788	38.59024144976839	21.867411234849214
40	24.937685205986497	19.704312290723923	39.06415481883244	16.293847684457138
41	18.901975887116617	20.927072969821452	40.877435090984946	19.29351605207698
42	19.55347304684575	21.121773270430158	41.73005124255164	17.594702440172448
43	19.2111428479733	19.463611369641733	42.12907988061234	19.19616590177263
44	21.25014709500733	20.390042470340298	39.4877884399371	18.872021994715276
45	18.405597098751564	20.52055585866042	39.51560276859548	21.558244273992532
46	19.674358398322582	21.364613755255306	38.68652181820127	20.274506028220845
47	17.391443884591933	19.890454336360815	44.29538817035206	18.422713608695187
48	18.802486173069312	21.985087240711618	39.136899986092835	20.075526600126235
49	18.18094290574152	20.345111631738288	40.8571092354269	20.616836227093298
50	18.235501781186816	19.280678669619263	40.80468992372455	21.67912962546937
51	18.482621393497865	20.020967724680936	39.88146816864042	21.61494271318078
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	24.0
1	135.0
2	246.0
3	5884.5
4	11523.0
5	7464.0
6	3405.0
7	3122.5
8	2840.0
9	2721.5
10	2603.0
11	2569.0
12	2535.0
13	2308.0
14	2081.0
15	1907.0
16	1733.0
17	1570.0
18	1407.0
19	1292.5
20	1178.0
21	1117.0
22	1056.0
23	980.0
24	904.0
25	902.0
26	945.5
27	991.0
28	1034.0
29	1077.0
30	1169.0
31	1261.0
32	1329.0
33	1397.0
34	1516.0
35	1635.0
36	1615.5
37	1596.0
38	1671.0
39	1746.0
40	1840.0
41	1934.0
42	2006.5
43	2079.0
44	2345.5
45	2612.0
46	5240.0
47	7868.0
48	6645.0
49	5422.0
50	5111.0
51	4800.0
52	4369.5
53	3939.0
54	3866.0
55	3793.0
56	3701.0
57	3609.0
58	3545.5
59	3482.0
60	3112.5
61	2743.0
62	2491.0
63	2239.0
64	2056.5
65	1874.0
66	1683.0
67	1492.0
68	1287.5
69	1083.0
70	970.5
71	858.0
72	707.5
73	557.0
74	487.5
75	343.0
76	268.0
77	200.5
78	133.0
79	105.5
80	78.0
81	57.5
82	37.0
83	25.5
84	14.0
85	9.5
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	93477.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.3812809568129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.99711046899311	58.619767429421145
2	8.971835010954814	12.09067471142634
3	2.23700504874099	4.521967970730769
4	0.7588988029085829	2.045422938262888
5	0.26355063029879655	0.8879189533254169
6	0.15876543993903405	0.6418691228858435
7	0.10637284475915282	0.5017276977224344
8	0.0349283967865875	0.18828160937984745
9	0.046041977582319885	0.27921306845534194
>10	0.37944940145429146	4.781924965499535
>50	0.02222716159146477	1.0259208147458734
>100	0.01746419839329375	1.8774671844410926
>500	0.0015876543993903407	0.7734522930774415
>1k	0.0031753087987806813	3.1419493565262044
>5k	0.0015876543993903407	8.622441884099832
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8060	8.622441884099832	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCG	1793	1.918118895557196	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGC	1144	1.2238304609690083	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTC	723	0.7734522930774415	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTACGCGTT	312	0.3337719439006387	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCT	212	0.22679375675299807	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTTACGCGTTCGTATGCCG	190	0.20325855558051714	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTC	179	0.19149095499427668	No Hit
CGCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	145	0.15511837136407886	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTT	131	0.14014142516340916	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCTTACGCGTTCGTATGC	128	0.13693207954897998	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGCGTTCGTATGCCGTCTTCTGC	128	0.13693207954897998	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGCGTTCGTA	120	0.1283738245771687	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	108	0.11553644211945184	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTTACGCGTTCGT	102	0.10911775089059342	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	94	0.10055949591878217	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02139563742952812	0.0
2	0.0	0.0	0.0	0.23000310236742727	0.0
3	0.0	0.0	0.0	0.3070273971137285	0.0
4	0.0	0.0	0.0	0.7049862533029515	0.0
5	0.0	0.0	0.0	1.8164896177669374	0.0
6	0.0	0.0	0.0	2.2722166950158864	0.0
7	0.0	0.0	0.0	2.6519892593900103	0.0
8	0.0	0.0	0.0	3.2029269232003594	0.0
9	0.0	0.0	0.0	3.5666527595023374	0.0
10	0.0	0.0	0.0	6.163013361575575	0.0
11	0.0	0.0	0.0	6.726788407843641	0.0
12	0.0	0.0	0.0	8.429881147234079	0.0
13	0.0	0.0	0.0	8.689838142002845	0.0
14	0.0	0.0	0.0	8.850305422724306	0.0
15	0.0	0.0	0.0	9.109192635621596	0.0
16	0.0	0.0	0.0	9.332777046760166	0.0
17	0.0	0.0	0.0	9.498593236839008	0.0
18	0.0	0.0	0.0	9.630176407030607	0.0
19	0.0	0.0	0.0	10.184323416455385	0.0
20	0.0	0.0	0.0	10.364046770863421	0.0
21	0.0	0.0	0.0	10.487071686083208	0.0
22	0.0	0.0	0.0	10.656097221776479	0.0
23	0.0	0.0	0.0	10.799447992554319	0.0
24	0.0	0.0	0.0	10.937449853974774	0.0
25	0.0	0.0	0.0	11.044428041122416	0.0
26	0.0	0.0	0.0	11.166383174470726	0.0
27	0.0	0.0	0.0	11.312943290862993	0.0
28	0.0	0.0	0.0	11.41778191426768	0.0
29	0.0	0.0	0.0	11.544016175101897	0.0
30	0.0	0.0	0.0	11.68308781839383	0.0
31	0.0	0.0	0.0	11.796484696770328	0.0
32	0.0	0.0	0.0	11.934486558190786	0.0
33	0.0	0.0	0.0	12.032906490366614	0.0
34	0.0	0.0	0.0	12.191234207345122	0.0
35	0.0	0.0	0.0	12.368817998010206	0.0
36	0.0	0.0	0.0	12.49505225884442	0.0
37	0.0	0.0	0.0	12.619146955935685	0.0
38	0.0	0.0	0.0	12.81384725654439	0.0
39	0.0	0.0	0.0	13.207526985247709	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATA	25	3.8632672E-5	45.000004	19
CGTGGGC	30	2.1440883E-6	45.000004	4
GATACAT	30	2.1440883E-6	45.000004	22
TCACAAT	30	2.1440883E-6	45.000004	41
TTATGGG	25	3.8632672E-5	45.000004	3
TGTGGGC	30	2.1440883E-6	45.000004	4
ACCCTTA	25	3.8632672E-5	45.000004	39
TTATCAC	30	2.1440883E-6	45.000004	38
TATTGGG	50	2.1827873E-11	45.000004	3
AATGATC	30	2.1440883E-6	45.000004	2
AACATGA	30	2.1440883E-6	45.000004	30
ACATGAT	30	2.1440883E-6	45.000004	31
AATTATC	30	2.1440883E-6	45.000004	45
TAGATAC	25	3.8632672E-5	45.000004	20
TGGGCTC	50	2.1827873E-11	45.000004	6
TGGGATC	35	1.1959855E-7	45.000004	6
ATACATC	30	2.1440883E-6	45.000004	23
TCAACAT	30	2.1440883E-6	45.000004	28
ACAACTA	20	7.0000323E-4	45.0	15
CCGGGAT	20	7.0000323E-4	45.0	5
>>END_MODULE