##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933254.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	861060
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.661347641279352	31.0	28.0	33.0	25.0	34.0
2	29.0860311708824	31.0	28.0	33.0	16.0	34.0
3	28.975116716605115	31.0	28.0	34.0	16.0	34.0
4	33.45571737161173	35.0	32.0	37.0	28.0	37.0
5	34.44445102548022	35.0	35.0	37.0	32.0	37.0
6	34.047719090423435	35.0	33.0	37.0	28.0	37.0
7	34.10830139595382	35.0	33.0	37.0	30.0	37.0
8	33.94986644368569	35.0	35.0	37.0	28.0	37.0
9	35.225546419529415	37.0	34.0	39.0	30.0	39.0
10	35.25571737161174	37.0	34.0	39.0	28.0	39.0
11	35.61482010545142	37.0	34.0	39.0	30.0	39.0
12	35.74667735117181	37.0	35.0	39.0	30.0	39.0
13	35.911881866536596	37.0	35.0	39.0	30.0	39.0
14	36.70529347548371	39.0	35.0	41.0	30.0	41.0
15	37.07753931201078	39.0	36.0	41.0	31.0	41.0
16	37.03247625020324	39.0	36.0	41.0	31.0	41.0
17	36.2001080064107	38.0	34.0	41.0	29.0	41.0
18	35.8571516502915	38.0	34.0	39.0	29.0	40.0
19	35.0283441339744	37.0	34.0	37.0	27.0	40.0
20	34.00516224188791	35.0	33.0	37.0	27.0	39.0
21	34.130306831115135	35.0	33.0	37.0	29.0	39.0
22	34.41285508559218	35.0	33.0	38.0	29.0	40.0
23	34.77009732190556	35.0	33.0	38.0	30.0	40.0
24	34.102335493461545	35.0	33.0	38.0	27.0	40.0
25	33.13838408473277	35.0	33.0	37.0	23.0	40.0
26	32.98465147608761	35.0	31.0	37.0	23.0	40.0
27	33.93965693447611	35.0	33.0	38.0	25.0	40.0
28	33.61787564165099	35.0	33.0	38.0	24.0	40.0
29	33.39634287970641	35.0	33.0	38.0	22.0	40.0
30	32.55182101131164	35.0	32.0	38.0	20.0	40.0
31	32.27297865421689	35.0	31.0	38.0	18.0	40.0
32	31.209762385896454	35.0	30.0	38.0	14.0	40.0
33	29.96803358650965	35.0	23.0	38.0	8.0	40.0
34	28.87208092351288	35.0	18.0	39.0	7.0	40.0
35	28.09727080575105	35.0	15.0	39.0	7.0	40.0
36	27.229828351102128	34.0	12.0	39.0	7.0	40.0
37	27.005839314333496	34.0	10.0	39.0	7.0	40.0
38	26.822511787796437	33.0	10.0	39.0	7.0	40.0
39	26.452954497944393	33.0	10.0	38.0	7.0	40.0
40	26.339315494855178	33.0	10.0	38.0	7.0	40.0
41	26.048622627923724	33.0	10.0	38.0	7.0	40.0
42	25.98955589622094	33.0	10.0	38.0	7.0	40.0
43	25.918891830999	33.0	10.0	38.0	7.0	40.0
44	25.866153345876015	33.0	10.0	38.0	7.0	40.0
45	25.932713167491233	33.0	10.0	38.0	7.0	40.0
46	25.72671939237684	32.0	10.0	38.0	7.0	40.0
47	25.563044387150722	32.0	10.0	38.0	7.0	40.0
48	25.437295891110956	32.0	10.0	38.0	7.0	40.0
49	25.50460827352333	32.0	10.0	38.0	7.0	40.0
50	25.473486168211274	32.0	10.0	38.0	7.0	40.0
51	24.634434301906953	30.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	13.0
10	33.0
11	59.0
12	74.0
13	110.0
14	166.0
15	289.0
16	572.0
17	1181.0
18	2496.0
19	4380.0
20	7201.0
21	11015.0
22	15886.0
23	24137.0
24	35179.0
25	51408.0
26	64595.0
27	62421.0
28	51085.0
29	41893.0
30	38247.0
31	38399.0
32	41129.0
33	46659.0
34	53081.0
35	61646.0
36	68135.0
37	72324.0
38	58065.0
39	9176.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.836294799433254	2.0018349476226978	25.757090098250995	51.40478015469305
2	50.24655656980931	12.798759668315796	25.69774464032704	11.256939121547859
3	8.891714863075743	13.085266996492695	66.16902422595405	11.853993914477504
4	7.61840057603419	2.0038092583559797	77.56997189510602	12.807818270503798
5	15.926067869834853	2.409239774231761	68.67779248832834	12.986899867605045
6	10.16990685898776	10.398926904048498	70.72561726244396	8.705548974519777
7	41.44612454416649	0.7059902910366292	56.00202076510348	1.8458643996934012
8	41.157178361554365	7.456739367756021	49.0027408078415	2.3833414628481173
9	38.78336004459619	1.9494576452279748	48.03544468445869	11.23173762571714
10	22.11704178570599	14.008431468190368	53.89032123196989	9.984205514133743
11	16.338582677165352	13.363993217661951	59.370427147933945	10.926996957238751
12	12.41133022089053	12.550693331475157	61.376326852948694	13.66164959468562
13	12.082665551761782	13.04543237405059	65.84326295496248	9.028639119225142
14	11.36273894966669	17.23445520637354	59.47622697605277	11.926578867906997
15	9.594337212273246	13.70880078043342	65.73014656353796	10.966715443755373
16	10.827816876872692	15.579634404106566	58.414860753025344	15.177687965995402
17	11.08540635960328	15.603558404756928	62.076742619561934	11.234292616077859
18	11.596172159895943	14.639281815436785	60.882633033702646	12.881912990964626
19	10.969502705967065	16.82275335052145	58.14449631849116	14.063247625020322
20	13.045200102199614	15.836643207209718	62.10136343576522	9.016793254825448
21	12.752421434046408	18.856641813578612	59.26044642649757	9.130490325877407
22	11.638677908624254	12.73174923930969	61.10445265138318	14.525120200682878
23	11.70661742503426	17.95031705107658	60.44863308015701	9.894432443732144
24	13.182821173901935	14.660883097577404	58.88799851346015	13.268297215060507
25	10.607623162148979	21.243583490116833	57.053515434464494	11.09527791326969
26	11.594546256939122	14.511532297400878	60.3853390007665	13.508582444893502
27	13.881030357930923	15.547813160523077	57.312846956077394	13.25830952546861
28	10.429470652451629	16.431607553480593	60.35049822312034	12.788423570947439
29	16.498153438784755	14.397719090423431	56.75365247485657	12.350474995935242
30	14.352658351334401	17.721993821568763	57.234803614149996	10.690544212946833
31	13.88230785311128	17.98388033354238	54.49794439411888	13.635867419227463
32	14.88432861821476	20.82874596427659	52.43606717301931	11.85085824448935
33	13.216152184516758	17.981557615032635	49.30469421410819	19.497595986342414
34	16.943999256730077	21.137202982370567	49.206210949295055	12.712586811604302
35	15.893898218474902	24.556825308340883	44.20969502705967	15.339581446124544
36	18.86256474577846	24.515597054792927	41.46575151557383	15.156086683854783
37	17.371495598448426	25.8005249343832	42.726987666364714	14.10099180080366
38	17.308433790908882	25.481383411144403	39.66854806865956	17.54163472928716
39	19.326179360323323	23.83236940515179	41.74842635820965	15.093024876315239
40	18.328455624462872	25.528070053190255	40.46454370194876	15.678930620398113
41	19.976656678977076	25.28476528929459	39.81859568438901	14.919982347339324
42	17.187884700253175	26.13999024458226	41.49699207952988	15.175132975634684
43	21.20525863470606	22.2719671103059	38.482684133509856	18.040090121478176
44	20.227161870252942	23.58221262165238	37.44280305669756	18.747822451397113
45	17.23886837154205	23.455508326945857	38.93596265068636	20.36966065082573
46	20.551064966436716	24.854017141662602	37.19055582653938	17.404362065361298
47	16.624160917938354	22.473230669175205	43.235663019998604	17.666945392887836
48	18.720995052609574	23.930852669964928	37.47532111583397	19.872831161591524
49	16.964206907764847	23.238101874433838	40.72317840800873	19.074512809792584
50	18.106287599005878	22.02401690939075	39.57633614382273	20.293359347780644
51	18.19780270828978	21.892551041739253	37.687965995401015	22.221680254569947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	51.0
1	1401.0
2	2751.0
3	38544.0
4	74337.0
5	55723.5
6	37110.0
7	35659.5
8	34209.0
9	33636.0
10	33063.0
11	32846.0
12	32629.0
13	31438.5
14	30248.0
15	28821.0
16	27394.0
17	26120.5
18	24847.0
19	23211.5
20	21576.0
21	19907.0
22	18238.0
23	17028.0
24	15818.0
25	15007.0
26	13812.0
27	13428.0
28	13702.0
29	13976.0
30	14512.5
31	15049.0
32	15843.5
33	16638.0
34	18023.0
35	19408.0
36	20540.5
37	21673.0
38	22568.5
39	23464.0
40	24510.0
41	25556.0
42	26181.0
43	26806.0
44	28520.5
45	30235.0
46	66365.5
47	102496.0
48	71826.0
49	41156.0
50	37867.5
51	34579.0
52	30686.5
53	26794.0
54	24777.5
55	22761.0
56	20640.5
57	18520.0
58	16565.5
59	14611.0
60	12554.5
61	10498.0
62	8708.0
63	6918.0
64	5544.0
65	4170.0
66	3169.5
67	2169.0
68	1692.0
69	1215.0
70	928.5
71	642.0
72	568.5
73	495.0
74	500.0
75	476.5
76	448.0
77	342.5
78	237.0
79	162.0
80	87.0
81	62.0
82	37.0
83	25.0
84	13.0
85	9.5
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	861060.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.24413815700673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.69961258011665	50.179555495413766
2	8.20607992420095	9.723242655136007
3	3.2561257550050327	5.78719092258318
4	1.5618964356284193	3.7013283287722576
5	0.8066043541678253	2.3893289898180914
6	0.4380903031841282	1.5572569468251274
7	0.26181032122036324	1.0857508788916657
8	0.16237453975808425	0.7695791733286661
9	0.10872329300549971	0.5797096012532292
>10	0.42121314819002437	4.356622266637146
>50	0.03351146418253739	1.3743266107287395
>100	0.039029867742394746	4.580087669082678
>500	0.002365446527089885	0.998956370553728
>1k	0.0017740848953174137	2.8158564422248644
>5k	0.0	0.0
>10k+	7.884821756966282E-4	10.101207648750906
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35278	4.0970431793370965	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	19866	2.3071562957285208	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	19244	2.2349197500754885	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	12108	1.406173785798899	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	4742	0.5507165586602559	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	3522	0.40903072956588393	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3105	0.3606020486377256	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT	2919	0.3390007664971082	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	2747	0.3190253873133115	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	2668	0.30985064919982347	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA	2265	0.26304787122848583	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGT	1102	0.1279817898868836	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	1042	0.1210136343576522	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	952	0.11056140106380508	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	904	0.10498687664041995	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03774417578333682	0.0
2	0.0	0.0	0.0	0.44503286646691287	0.0
3	0.0	0.0	0.0	0.5274893735628179	0.0
4	0.0	0.0	0.0	1.1953870810396487	0.0
5	0.0	0.0	0.0	3.896128028244257	0.0
6	0.0	0.0	0.0	4.3541681183657355	0.0
7	0.0	0.0	0.0	4.8313706361926	0.0
8	0.0	0.0	0.0	5.414372982138294	0.0
9	0.0	0.0	0.0	5.568485355259796	0.0
10	0.0	0.0	0.0	8.233688709265325	0.0
11	0.0	0.0	0.0	8.384200868696723	0.0
12	0.0	0.0	0.0	10.130769052098577	0.0
13	0.0	0.0	0.0	10.300095231458899	0.0
14	1.161359254871902E-4	0.0	0.0	10.425522030985064	0.0
15	1.161359254871902E-4	0.0	0.0	10.781246370752328	0.0
16	1.161359254871902E-4	0.0	0.0	10.980535618888347	0.0
17	1.161359254871902E-4	0.0	0.0	11.0825029614661	0.0
18	1.161359254871902E-4	0.0	0.0	11.155320186746568	0.0
19	1.161359254871902E-4	0.0	0.0	11.634380879381228	0.0
20	2.322718509743804E-4	0.0	0.0	11.734838454927647	0.0
21	2.322718509743804E-4	0.0	0.0	11.799874573200475	0.0
22	2.322718509743804E-4	0.0	0.0	11.949341509302487	0.0
23	2.322718509743804E-4	0.0	0.0	12.017629433488956	0.0
24	2.322718509743804E-4	0.0	0.0	12.082665551761782	0.0
25	2.322718509743804E-4	0.0	0.0	12.139107611548557	0.0
26	2.322718509743804E-4	0.0	0.0	12.223190021601281	0.0
27	2.322718509743804E-4	0.0	0.0	12.347571597798062	0.0
28	2.322718509743804E-4	0.0	0.0	12.41713701716489	0.0
29	2.322718509743804E-4	0.0	0.0	12.492276960955103	0.0
30	2.322718509743804E-4	0.0	0.0	12.578565953592085	0.0
31	2.322718509743804E-4	0.0	0.0	12.659396557731169	0.0
32	2.322718509743804E-4	0.0	0.0	12.747079181473998	0.0
33	2.322718509743804E-4	0.0	0.0	12.834064989663903	0.0
34	2.322718509743804E-4	0.0	0.0	12.942768215919912	0.0
35	2.322718509743804E-4	0.0	0.0	13.040322393329152	0.0
36	2.322718509743804E-4	0.0	0.0	13.127540473370033	0.0
37	2.322718509743804E-4	0.0	0.0	13.229159408171324	0.0
38	2.322718509743804E-4	0.0	0.0	13.339372401458668	0.0
39	2.322718509743804E-4	0.0	0.0	13.470605997259192	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCGCG	25	3.889988E-5	45.0	1
GCGATTA	35	1.211447E-7	45.0	9
GGCTGCG	20	7.0323E-4	45.0	1
CTAGCGG	20	7.0323E-4	45.0	2
CGTTTTT	35030	0.0	44.59535	1
ACGGCTG	1480	0.0	43.631756	8
TACGGCT	1490	0.0	43.489933	7
CGGCTGT	1515	0.0	43.069305	9
GATACCT	2280	0.0	42.335526	5
CGACCTG	140	0.0	41.785713	12
TGATACC	2360	0.0	41.5678	4
ATACCTG	2330	0.0	41.42704	6
GAATCTG	2560	0.0	41.132812	1
CGCTTTT	860	0.0	40.813953	1
CCGTTTG	50	1.0804797E-9	40.5	1
CCGATGA	495	0.0	40.454544	18
TACCTGT	2435	0.0	40.287476	7
GGCGACT	320	0.0	40.078125	10
ATGATAC	6185	0.0	40.016167	3
AGCGACT	45	1.9274012E-8	40.000004	19
>>END_MODULE