FastQCFastQC Report
Sat 14 Jan 2017
SRR2933253.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933253.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505349
Sequences flagged as poor quality0
Sequence length51
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT333446.598212324551943No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG81931.621255805393896No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC53141.0515505126160336No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC44060.8718727057934219No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT16600.32848585828803456No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC12790.2530924173195158No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT11440.22637820595271782No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC10510.20797508256670144No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT8340.16503446133266317No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGAACTAGTCGTATGCCG7020.13891389910734958No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6740.1333731737868285No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA6320.1250620858060469No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT6300.12466631971172397No Hit
GAATCTTTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC5720.11318910297635891No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA5640.11160603859906718No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5430.10745049460867638No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC5150.10190976928815532No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5150.10190976928815532No Hit
GAATGCTGCCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT5140.10171188624099384No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT5080.10052458795802505No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTGC700.045.0000049
TTGTGCG406.8012014E-945.01
CGACCCT207.02953E-445.012
TTCGTCG207.02953E-445.01
CTACGGG207.02953E-445.03
TCTCGCG253.887692E-545.01
TCGTCGG207.02953E-445.02
CGGTGTC406.8012014E-945.019
CGTTTTT213200.044.725611
CACCGGT850.042.3529416
GATACCT10850.042.3041465
GACCGCC700.041.78571713
CTACGGC700.041.7857176
TGATACC11050.041.5384644
GGACCGA1200.041.2500048
ACGGCTG6500.041.192318
GTTTTTT236300.040.572372
TACGGCT6600.040.5681847
ATACCTG11200.040.3794676
GAATGAC1450.040.3448261
Produced by FastQC (version 0.11.5)