##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933253.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	505349
Sequences flagged as poor quality	0
Sequence length	51
%GC	31
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.96228744887197	31.0	28.0	31.0	16.0	33.0
2	29.284171928706694	31.0	30.0	33.0	16.0	34.0
3	29.363881198933807	31.0	30.0	33.0	16.0	34.0
4	32.64586849879984	35.0	32.0	37.0	19.0	37.0
5	33.969148054117056	35.0	33.0	37.0	30.0	37.0
6	33.35824944741159	35.0	35.0	37.0	26.0	37.0
7	32.87890744812001	35.0	32.0	37.0	27.0	37.0
8	33.509726941183224	35.0	33.0	37.0	28.0	37.0
9	33.70674523942859	35.0	32.0	39.0	26.0	39.0
10	32.902396165818075	37.0	31.0	39.0	17.0	39.0
11	33.76625658703193	37.0	32.0	39.0	23.0	39.0
12	34.30438963963518	37.0	32.0	39.0	27.0	39.0
13	34.57598610069477	37.0	32.0	39.0	27.0	39.0
14	34.65938588975144	38.0	32.0	40.0	24.0	41.0
15	35.27863516104712	38.0	32.0	40.0	27.0	41.0
16	35.44617482175685	38.0	33.0	40.0	27.0	41.0
17	33.60969152011778	37.0	31.0	40.0	19.0	41.0
18	33.975927527312805	37.0	32.0	39.0	25.0	39.0
19	33.57961329694924	37.0	32.0	37.0	25.0	39.0
20	32.83232775764868	35.0	32.0	35.0	25.0	39.0
21	32.914655020589734	35.0	32.0	36.0	25.0	39.0
22	32.62205525290443	34.0	30.0	37.0	25.0	39.0
23	33.76405414871702	35.0	33.0	38.0	27.0	39.0
24	31.940160166538373	34.0	31.0	36.0	21.0	39.0
25	31.108855464243522	34.0	28.0	36.0	18.0	39.0
26	29.766945220036053	33.0	24.0	36.0	18.0	39.0
27	32.01993275934058	34.0	30.0	37.0	22.0	39.0
28	32.11475237904894	35.0	31.0	37.0	21.0	39.0
29	31.92857807178801	35.0	30.0	37.0	21.0	39.0
30	30.997825265311697	34.0	27.0	37.0	18.0	39.0
31	31.37190139883526	34.0	30.0	36.0	18.0	39.0
32	30.871275098990996	35.0	29.0	37.0	15.0	39.0
33	30.034061608907905	35.0	25.0	37.0	10.0	40.0
34	28.46952700015237	34.0	20.0	37.0	7.0	40.0
35	27.589515364629197	33.0	18.0	37.0	7.0	40.0
36	26.736811589614305	33.0	15.0	37.0	7.0	40.0
37	26.699839121082658	33.0	12.0	37.0	7.0	40.0
38	26.698744827831856	33.0	12.0	37.0	7.0	40.0
39	26.40199941030852	33.0	12.0	37.0	7.0	40.0
40	26.292623513650963	33.0	12.0	37.0	7.0	40.0
41	25.979954447322545	32.0	11.0	37.0	7.0	40.0
42	26.11206116960754	32.0	10.0	37.0	7.0	40.0
43	26.164828662963615	33.0	10.0	37.0	7.0	40.0
44	26.023819182386827	32.0	10.0	37.0	7.0	40.0
45	26.14504431590841	33.0	10.0	38.0	7.0	40.0
46	25.912333852446526	32.0	10.0	37.0	7.0	40.0
47	25.88470344257137	32.0	10.0	37.0	7.0	40.0
48	25.62300311270033	32.0	10.0	37.0	7.0	40.0
49	25.272860933730946	31.0	10.0	36.0	7.0	39.0
50	25.455904731185775	32.0	10.0	37.0	7.0	39.0
51	24.293260697062824	29.0	10.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	11.0
11	17.0
12	28.0
13	53.0
14	115.0
15	263.0
16	532.0
17	1102.0
18	2063.0
19	3410.0
20	5592.0
21	8291.0
22	11487.0
23	16207.0
24	22609.0
25	30886.0
26	36838.0
27	34774.0
28	30186.0
29	27515.0
30	28196.0
31	30663.0
32	34280.0
33	37831.0
34	41722.0
35	44556.0
36	33398.0
37	18615.0
38	4028.0
39	77.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	17.820159929078716	1.9548866229081288	28.69264607231834	51.53230737569482
2	52.01217376506138	9.233420863601193	27.50594143849102	11.248463932846409
3	9.249647273468435	9.274184771316458	69.70074146777772	11.775426487437395
4	8.215906235096933	2.0577858074320914	76.22573706487992	13.50057089259106
5	14.259650261502447	2.445636579868566	70.28706893651714	13.007644222111846
6	10.757120326744488	7.646992474507716	71.87508038998791	9.720806808759887
7	42.97999996042339	0.7016932852345606	54.085196567124896	2.2331101872171506
8	44.31036768648993	5.40913309415869	47.750564461392024	2.5299347579593507
9	41.19786523768722	1.9000730188444024	47.42346378443412	9.478597959034252
10	21.570439438882833	15.537381097024037	52.83793972086618	10.054239743226958
11	15.14240653488975	14.033469938596891	59.957969640782906	10.866153885730457
12	12.366107383214372	13.096295827240183	61.35403453850705	13.183562251038392
13	11.798380920908125	13.527087220910698	64.88565328119775	9.788878576983432
14	11.350373702134565	15.385209033756869	60.54785900437124	12.71655825973733
15	9.97073309732482	14.301997233595001	64.4354693489054	11.29180032017477
16	11.172278959689244	14.792747190555438	59.11498785987506	14.91998598988026
17	11.308620379183495	15.13449121300329	61.966284686424636	11.590603721388584
18	11.574575194568506	14.380754686365266	60.74138862449515	13.30328149457108
19	11.254598307308415	17.060289027978683	58.07412303180575	13.610989632907158
20	12.93818727255817	16.306354618293494	61.05067982720852	9.704778281939808
21	13.031785953865546	18.133013026640995	58.657086488743424	10.178114530750037
22	11.7168531054776	14.017441411776813	60.246087357449994	14.019618125295588
23	11.625629020736165	18.008544589976434	59.41181243061726	10.954013958670147
24	12.363732786648434	15.247482432932488	59.02712778693536	13.361656993483711
25	11.156250432869165	18.977379988878972	57.30495162748912	12.56141795076274
26	11.733871047533487	15.8266861119741	59.548945382300154	12.89049745819226
27	12.674607053739098	16.011113111928587	57.68053365100159	13.633746183330727
28	10.906324144304232	16.692424443305516	59.808963706270326	12.592287706119928
29	15.288048457600588	14.79136200922531	57.09618501273377	12.82440452044033
30	13.943631035185586	16.961545387445113	57.49768971542439	11.597133861944913
31	13.64324456959448	17.465157742471042	55.57090248521319	13.32069520272129
32	14.222844014730414	19.528286392176497	54.10359969051092	12.145269902582175
33	12.772757045131186	18.10570516613271	51.52122592505377	17.60031186368233
34	16.099764717056924	21.22374834025594	49.39061915626626	13.28586778642087
35	15.034362391139588	23.90803187500124	45.366865275285	15.690740458574174
36	17.398669038624792	24.043581762306843	43.030460137449566	15.527289061618802
37	16.791959616027736	25.27243548517955	43.248725138468664	14.686879760324054
38	16.757527965821637	24.4535954360254	42.148297513203744	16.640579084949213
39	18.11223530668904	23.22335653182256	43.46857320386505	15.195834957623344
40	18.03110325735284	24.45676156477998	42.06419721816012	15.447937959707053
41	19.240762324650884	23.933360905037905	42.04144066773656	14.784436102574656
42	17.170707768294783	24.856485320046147	42.92558212245399	15.047224789205085
43	19.906243012254897	22.021414903363816	40.86245347274854	17.209888611632753
44	19.365032878268284	23.095721966403417	39.855624528791	17.683620626537305
45	16.93423752693683	22.921782767948486	40.805265272118874	19.33871443299581
46	18.966496421285093	24.106706454351347	39.181239104064716	17.745558020298844
47	16.588733726592906	21.789298089043413	43.71216723492082	17.90980094944286
48	18.04060164361659	22.831350215395695	39.8324722122731	19.295575928714612
49	16.46485893906983	22.62654126158358	41.07260526883401	19.835994530512576
50	17.423008653425654	21.2583778735092	41.15729921301912	20.16131426004603
51	17.61673615659673	21.105612161100545	39.89322230775167	21.384429374551054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53.0
1	332.5
2	612.0
3	26801.5
4	52991.0
5	37233.0
6	21475.0
7	20384.0
8	19293.0
9	19048.5
10	18804.0
11	18643.5
12	18483.0
13	17729.5
14	16976.0
15	16036.5
16	15097.0
17	14455.5
18	13814.0
19	12708.5
20	11603.0
21	10742.5
22	9882.0
23	9211.5
24	8541.0
25	8105.5
26	7525.0
27	7380.0
28	7710.0
29	8040.0
30	8357.0
31	8674.0
32	9249.0
33	9824.0
34	10699.0
35	11574.0
36	12442.5
37	13311.0
38	13853.0
39	14395.0
40	14993.5
41	15592.0
42	16486.5
43	17381.0
44	18555.0
45	19729.0
46	32435.0
47	45141.0
48	37034.0
49	28927.0
50	26486.0
51	24045.0
52	21007.5
53	17970.0
54	15964.5
55	13959.0
56	12365.0
57	10771.0
58	9402.5
59	8034.0
60	6823.5
61	5613.0
62	4698.5
63	3784.0
64	2982.5
65	2181.0
66	1694.0
67	1207.0
68	929.0
69	651.0
70	527.5
71	404.0
72	383.0
73	362.0
74	385.5
75	397.5
76	386.0
77	297.5
78	209.0
79	132.5
80	56.0
81	41.0
82	26.0
83	19.0
84	12.0
85	8.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	505349.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.319241903771335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.27620060128906	52.29071973304975
2	8.287822360707786	10.16405968383452
3	3.1220372956900633	5.743228805010453
4	1.3937836691348493	3.418630318768232
5	0.6836767100851122	2.096126878484177
6	0.36994004411591774	1.361066583302148
7	0.22069185518925494	0.9472860078179398
8	0.12840331395601193	0.6298875095771675
9	0.07771038533005072	0.42886277248397975
>10	0.3538100862890034	3.987734633409157
>50	0.04066616843261976	1.7950032068063955
>100	0.03869845039183458	4.348750189320909
>500	0.003935435633067923	1.4485108723088327
>1k	0.0016397648471116345	1.918477540167467
>5k	6.559059388446539E-4	2.716234395706706
>10k+	3.279529694223269E-4	6.705420869952203
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33344	6.598212324551943	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCG	8193	1.621255805393896	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	5314	1.0515505126160336	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	4406	0.8718727057934219	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	1660	0.32848585828803456	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1279	0.2530924173195158	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAACTAGT	1144	0.22637820595271782	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTC	1051	0.20797508256670144	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	834	0.16503446133266317	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGAACTAGTCGTATGCCG	702	0.13891389910734958	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	674	0.1333731737868285	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTA	632	0.1250620858060469	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	630	0.12466631971172397	No Hit
GAATCTTTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTC	572	0.11318910297635891	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	564	0.11160603859906718	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	543	0.10745049460867638	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	515	0.10190976928815532	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	515	0.10190976928815532	No Hit
GAATGCTGCCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTT	514	0.10171188624099384	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCT	508	0.10052458795802505	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06094797852573172	0.0
2	0.0	0.0	0.0	0.4199078260766322	0.0
3	0.0	0.0	0.0	0.5564471286180441	0.0
4	0.0	0.0	0.0	1.1502941531496056	0.0
5	0.0	0.0	0.0	2.6314487611531834	0.0
6	0.0	0.0	0.0	3.2148079841851867	0.0
7	0.0	0.0	0.0	3.7524562233228917	0.0
8	0.0	0.0	0.0	4.355207984976719	0.0
9	0.0	0.0	0.0	4.7111995868201975	0.0
10	0.0	0.0	0.0	6.897807257954404	0.0
11	0.0	0.0	0.0	7.323255809351557	0.0
12	0.0	0.0	0.0	8.625722025768331	0.0
13	0.0	0.0	0.0	8.851704465626725	0.0
14	0.0	0.0	0.0	8.98606705464936	0.0
15	0.0	0.0	0.0	9.241336185487652	0.0
16	0.0	0.0	0.0	9.41230713823516	0.0
17	0.0	0.0	0.0	9.570613575964334	1.9788304716146664E-4
18	0.0	0.0	0.0	9.721400457901371	1.9788304716146664E-4
19	0.0	0.0	0.0	10.126862821535216	1.9788304716146664E-4
20	0.0	0.0	0.0	10.269140732444312	1.9788304716146664E-4
21	0.0	0.0	0.0	10.410231345070436	1.9788304716146664E-4
22	0.0	0.0	0.0	10.594658345024923	1.9788304716146664E-4
23	0.0	0.0	0.0	10.743070630396023	1.9788304716146664E-4
24	0.0	0.0	0.0	10.862987756975873	1.9788304716146664E-4
25	0.0	0.0	0.0	10.9773641582352	1.9788304716146664E-4
26	0.0	0.0	0.0	11.10024953052247	1.9788304716146664E-4
27	0.0	0.0	0.0	11.219770891007997	1.9788304716146664E-4
28	0.0	0.0	0.0	11.328606566946803	1.9788304716146664E-4
29	0.0	0.0	0.0	11.451096173139751	1.9788304716146664E-4
30	0.0	0.0	0.0	11.587635475681163	1.9788304716146664E-4
31	0.0	0.0	0.0	11.713489093675856	1.9788304716146664E-4
32	0.0	0.0	0.0	11.853788174113335	1.9788304716146664E-4
33	0.0	0.0	0.0	11.986369815711518	1.9788304716146664E-4
34	0.0	0.0	0.0	12.12429429958306	1.9788304716146664E-4
35	0.0	0.0	0.0	12.272112935812675	1.9788304716146664E-4
36	0.0	0.0	0.0	12.407662823118281	1.9788304716146664E-4
37	0.0	0.0	0.0	12.555679342395058	1.9788304716146664E-4
38	0.0	0.0	0.0	12.722098985057851	1.9788304716146664E-4
39	0.0	0.0	0.0	12.904942920635046	1.9788304716146664E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCTGC	70	0.0	45.000004	9
TTGTGCG	40	6.8012014E-9	45.0	1
CGACCCT	20	7.02953E-4	45.0	12
TTCGTCG	20	7.02953E-4	45.0	1
CTACGGG	20	7.02953E-4	45.0	3
TCTCGCG	25	3.887692E-5	45.0	1
TCGTCGG	20	7.02953E-4	45.0	2
CGGTGTC	40	6.8012014E-9	45.0	19
CGTTTTT	21320	0.0	44.72561	1
CACCGGT	85	0.0	42.35294	16
GATACCT	1085	0.0	42.304146	5
GACCGCC	70	0.0	41.785717	13
CTACGGC	70	0.0	41.785717	6
TGATACC	1105	0.0	41.538464	4
GGACCGA	120	0.0	41.250004	8
ACGGCTG	650	0.0	41.19231	8
GTTTTTT	23630	0.0	40.57237	2
TACGGCT	660	0.0	40.568184	7
ATACCTG	1120	0.0	40.379467	6
GAATGAC	145	0.0	40.344826	1
>>END_MODULE