##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933252.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	396214
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.507208225857745	31.0	28.0	33.0	25.0	34.0
2	28.86952253075358	31.0	28.0	33.0	16.0	34.0
3	28.86138551388896	31.0	28.0	34.0	16.0	34.0
4	33.20602755076802	35.0	32.0	37.0	28.0	37.0
5	34.224896141983876	35.0	33.0	37.0	30.0	37.0
6	33.83198978329892	35.0	33.0	37.0	28.0	37.0
7	33.60043562317333	35.0	32.0	37.0	29.0	37.0
8	33.62744627903103	35.0	33.0	37.0	28.0	37.0
9	34.755197948583344	37.0	34.0	39.0	28.0	39.0
10	34.46701529981273	37.0	32.0	39.0	27.0	39.0
11	34.61298692120925	37.0	33.0	39.0	27.0	39.0
12	34.697729005032635	37.0	33.0	39.0	27.0	39.0
13	34.83132095281842	37.0	34.0	39.0	27.0	39.0
14	35.70446021594391	38.0	34.0	40.0	27.0	41.0
15	36.024368144487575	38.0	34.0	40.0	27.0	41.0
16	35.92821303638942	38.0	34.0	40.0	27.0	41.0
17	35.221536846249755	37.0	33.0	40.0	27.0	41.0
18	34.96489270949537	37.0	33.0	39.0	27.0	40.0
19	34.24943086311943	37.0	33.0	37.0	27.0	40.0
20	33.60974877212819	35.0	32.0	37.0	27.0	39.0
21	33.881700293275856	35.0	33.0	38.0	27.0	40.0
22	34.435873038307584	35.0	33.0	38.0	28.0	40.0
23	34.83190144719773	35.0	33.0	39.0	30.0	40.0
24	34.25528628468454	35.0	33.0	38.0	27.0	40.0
25	33.353521076993744	35.0	32.0	38.0	24.0	40.0
26	33.30281363101758	35.0	32.0	38.0	24.0	40.0
27	34.00895728066146	35.0	33.0	38.0	25.0	40.0
28	33.798974796448384	35.0	33.0	38.0	24.0	40.0
29	33.794469655287294	35.0	33.0	39.0	24.0	40.0
30	33.020340018272954	35.0	32.0	38.0	21.0	40.0
31	32.88339129864164	35.0	32.0	38.0	21.0	40.0
32	32.21006072475985	35.0	31.0	38.0	18.0	40.0
33	31.57258451240996	35.0	30.0	39.0	13.0	40.0
34	30.945112994492874	35.0	28.0	39.0	10.0	40.0
35	30.295353016299273	35.0	24.0	39.0	8.0	40.0
36	29.76791834715583	35.0	23.0	39.0	7.0	40.0
37	29.588964549460645	35.0	22.0	39.0	7.0	40.0
38	29.29176657059064	35.0	21.0	39.0	7.0	40.0
39	28.92242828370527	35.0	20.0	38.0	7.0	40.0
40	28.568081895137475	34.0	18.0	38.0	7.0	40.0
41	28.15424240435724	34.0	18.0	38.0	7.0	40.0
42	28.31414589085696	34.0	18.0	38.0	7.0	40.0
43	28.177328413433145	34.0	18.0	38.0	7.0	40.0
44	28.24755308999682	34.0	18.0	38.0	7.0	40.0
45	28.30362127537139	34.0	18.0	38.0	7.0	40.0
46	28.1246195237927	34.0	18.0	38.0	7.0	40.0
47	27.950362682792633	34.0	18.0	38.0	7.0	40.0
48	27.86547168954151	34.0	18.0	38.0	7.0	40.0
49	27.981843145370934	34.0	18.0	38.0	7.0	40.0
50	28.006915454779488	34.0	18.0	38.0	7.0	40.0
51	26.733838279313705	32.0	16.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	30.0
10	40.0
11	30.0
12	34.0
13	37.0
14	67.0
15	158.0
16	208.0
17	465.0
18	953.0
19	1638.0
20	2590.0
21	4063.0
22	5736.0
23	8360.0
24	11723.0
25	17221.0
26	21772.0
27	23081.0
28	21275.0
29	19768.0
30	20078.0
31	21473.0
32	24670.0
33	27839.0
34	30022.0
35	33699.0
36	35570.0
37	34721.0
38	25276.0
39	3608.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.180584229734436	2.28588591013947	31.240188383045524	41.29334147708057
2	40.46247734809976	14.750614566875475	31.02818174017072	13.758726344854043
3	11.894077442997975	14.957321043678414	59.060002927710784	14.088598585612825
4	10.456470493218312	2.761134134583836	70.60023118819629	16.182164184001575
5	19.325919831202327	3.2015526962701975	60.6598959148339	16.81263155769357
6	13.562367811334278	12.171452800759186	63.3501087795989	10.916070608307631
7	50.87654651274312	1.2102045864103743	45.37144068609388	2.5418082147526335
8	50.22235458615798	8.803071067655358	37.87852019363274	3.096054152553923
9	47.32871629977739	2.3835603991782217	36.93660496600322	13.351118335041164
10	31.07689279025981	14.457843488619787	42.71025254029388	11.755011180826523
11	25.57254413019227	13.785227175213393	47.091723159706625	13.550505534887714
12	19.67295451447955	12.92937654903663	50.903299731963024	16.49436920452079
13	18.230047398628013	13.652975412277204	56.09695770467474	12.020019484420036
14	14.885137829556754	18.31207377831172	51.7220491956367	15.080739196494825
15	13.323860338100118	12.95663454597768	58.60746970071729	15.112035415204913
16	16.606177469751195	15.612774914566371	48.54220194137512	19.23884567430732
17	16.17560207362688	16.005239592745333	52.58471432104873	15.23444401257906
18	17.22074434522758	14.58832852953202	50.94772017142252	17.243206953817886
19	16.025935479311684	17.68084923803803	47.99199422534287	18.301221057307416
20	17.924656877344063	18.69242379118355	51.78565118849915	11.597268142973242
21	17.10287874734361	20.84353404978118	49.93135022992625	12.122236972948961
22	15.166046631365878	13.161321911896096	53.21038630638998	18.462245150348046
23	16.268228785454326	18.65380829551707	52.138238426708796	12.939724492319806
24	18.249228952030975	16.18872629437627	48.876112403902944	16.685932349689814
25	16.436067377730215	22.395220764536337	45.82675019055359	15.341961667179858
26	15.22434845815645	14.175925131368402	50.601695043587554	19.99803136688759
27	17.195505459171052	15.328080279848768	48.96672000484587	18.509694256134313
28	13.615117083192416	17.891341547749448	50.167586203415325	18.32595516564281
29	20.575749468721447	15.751084010156127	46.891578793278384	16.781587727844045
30	17.592765525700756	17.708107234979074	51.81467590746415	12.884451331856017
31	22.566340411999576	14.504283038963791	45.62635343526478	17.30302311377185
32	21.339478160791895	17.695235403090248	44.02847955902619	16.936806877091673
33	19.405927100001513	19.33601538562494	41.14140338302029	20.116654131353258
34	24.443608756883904	18.68359018106377	42.588348720640866	14.28445234141146
35	16.596586693049716	22.65215262459176	44.23266214722347	16.518598535135055
36	23.218765616560745	25.853200543140826	35.373055974801495	15.554977865496927
37	19.127542186798046	22.4136451513576	39.93018924116765	18.528623420676706
38	22.936594870448797	24.790643440161126	35.15171094408577	17.121050745304306
39	25.08997662879151	20.985628978279415	34.303179594865405	19.621214798063672
40	21.82381238421661	22.381339377205247	39.23081970854134	16.564028530036797
41	21.489901921688784	25.810042047984172	33.77265820995724	18.9273978203698
42	19.277965947694934	21.938396926913235	40.528603229567864	18.255033895823974
43	21.97474092283463	22.01083252989546	34.76101298793077	21.253413559339148
44	22.927256482607884	20.910669486691535	34.46647518765112	21.695598843049464
45	20.585592634283493	20.313012664873025	34.09218250743285	25.00921219341063
46	23.278581776514713	22.691020509118808	32.679814443709716	21.350583270656767
47	16.670031851474203	22.627418516256366	40.96927418011479	19.733275452154643
48	20.22089073076671	23.959526922319757	33.44303835805903	22.376543988854507
49	19.20376362268875	20.977047757020195	38.83204530884825	20.987143311442807
50	19.37538804787312	20.803404220951304	36.5118345136719	23.309373217503673
51	19.29134255730489	20.26808744769241	34.7600034324885	25.680566562514194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	39.0
1	712.0
2	1385.0
3	15406.5
4	29428.0
5	21513.0
6	13598.0
7	12937.0
8	12276.0
9	11813.5
10	11351.0
11	10674.5
12	9998.0
13	9324.5
14	8651.0
15	7926.0
16	7201.0
17	6692.0
18	6183.0
19	5730.5
20	5278.0
21	4998.5
22	4719.0
23	4469.5
24	4220.0
25	4299.0
26	4501.0
27	4624.0
28	4966.0
29	5308.0
30	5531.5
31	5755.0
32	6031.5
33	6308.0
34	6761.0
35	7214.0
36	7363.5
37	7513.0
38	7771.0
39	8029.0
40	8390.5
41	8752.0
42	9088.5
43	9425.0
44	10825.5
45	12226.0
46	30555.0
47	48884.0
48	34835.5
49	20787.0
50	19924.0
51	19061.0
52	18069.0
53	17077.0
54	17323.5
55	17570.0
56	17264.5
57	16959.0
58	16303.0
59	15647.0
60	14111.0
61	12575.0
62	11419.5
63	10264.0
64	8860.0
65	7456.0
66	6204.5
67	4953.0
68	4211.5
69	3470.0
70	2972.5
71	2475.0
72	2180.0
73	1885.0
74	1639.0
75	1186.0
76	979.0
77	739.0
78	499.0
79	374.0
80	249.0
81	171.0
82	93.0
83	66.5
84	40.0
85	28.5
86	17.0
87	15.5
88	14.0
89	9.0
90	4.0
91	3.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	396214.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.74671908907723
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.5254022273344	44.0850157527483
2	9.437539085611245	10.333486024242914
3	4.350140603667008	7.144677768808395
4	2.3334595706001147	5.109970224694529
5	1.217873335603486	3.333728469518076
6	0.6796599043144309	2.2325489914566754
7	0.37292449953652085	1.4291474972292433
8	0.22497437715498586	0.9853287222675301
9	0.13556441055746868	0.6679536032939467
>10	0.6177467796658503	6.051360215032064
>50	0.06404456792752498	2.395241334644817
>100	0.035060894850809085	3.2262434303361114
>500	4.674785980107878E-4	0.15893203000465853
>1k	0.0032723501860755146	2.7888604363297325
>5k	9.349571960215756E-4	3.723513855938449
>10k+	9.349571960215756E-4	6.3339916434545795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14288	3.6061320397562935	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	10461	2.6402398703730814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	7987	2.0158298293346526	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	6562	1.656175703029171	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCT	2597	0.6554538708879545	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTC	1729	0.4363803399173174	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGCAGTAT	1480	0.3735355136365701	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1447	0.3652066812379169	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCT	1260	0.3180099643122151	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGCAGTATCGTA	1193	0.3010999106543434	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTT	1191	0.3005951329332129	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGT	621	0.1567334824110203	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	481	0.12139904193188529	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	447	0.1128178206726668	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	431	0.10877959890362278	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.5238886056525007E-4	0.0	0.0	0.04139177313270102	0.0
2	2.5238886056525007E-4	0.0	0.0	0.4631335591372339	0.0
3	2.5238886056525007E-4	0.0	0.0	0.5597984927337247	0.0
4	2.5238886056525007E-4	0.0	0.0	1.351289959466349	0.0
5	2.5238886056525007E-4	0.0	0.0	4.504383994508018	0.0
6	2.5238886056525007E-4	0.0	0.0	4.951869444290207	0.0
7	2.5238886056525007E-4	0.0	0.0	5.437465612017748	0.0
8	2.5238886056525007E-4	0.0	0.0	6.076766595829526	0.0
9	2.5238886056525007E-4	0.0	0.0	6.254195964806898	0.0
10	2.5238886056525007E-4	0.0	0.0	8.924217720726679	0.0
11	2.5238886056525007E-4	0.0	0.0	9.140767363091662	0.0
12	2.5238886056525007E-4	0.0	0.0	11.335288505706512	0.0
13	2.5238886056525007E-4	0.0	0.0	11.515746541010666	0.0
14	2.5238886056525007E-4	0.0	0.0	11.652288914576467	0.0
15	2.5238886056525007E-4	0.0	0.0	12.06014931324991	0.0
16	2.5238886056525007E-4	0.0	0.0	12.301685452810855	0.0
17	2.5238886056525007E-4	0.0	0.0	12.424851216766697	0.0
18	2.5238886056525007E-4	0.0	0.0	12.502334596960228	0.0
19	2.5238886056525007E-4	0.0	0.0	13.044718258314951	0.0
20	5.047777211305001E-4	0.0	0.0	13.166622077967967	0.0
21	5.047777211305001E-4	0.0	0.0	13.251172346257325	0.0
22	5.047777211305001E-4	0.0	0.0	13.448540435219352	0.0
23	5.047777211305001E-4	0.0	0.0	13.529052481739665	0.0
24	5.047777211305001E-4	0.0	0.0	13.615874249774112	0.0
25	5.047777211305001E-4	0.0	0.0	13.673418909982988	0.0
26	5.047777211305001E-4	0.0	0.0	13.773112509906262	0.0
27	5.047777211305001E-4	0.0	0.0	13.935650936110283	0.0
28	5.047777211305001E-4	0.0	0.0	14.015910593770034	0.0
29	5.047777211305001E-4	0.0	0.0	14.099956084338261	0.0
30	5.047777211305001E-4	0.0	0.0	14.216559737919408	0.0
31	5.047777211305001E-4	0.0	0.0	14.304895839117245	0.0
32	5.047777211305001E-4	0.0	0.0	14.421751881558956	0.0
33	5.047777211305001E-4	0.0	0.0	14.521193092621663	0.0
34	5.047777211305001E-4	0.0	0.0	14.691555573503209	0.0
35	5.047777211305001E-4	0.0	0.0	14.832388557698618	0.0
36	5.047777211305001E-4	0.0	0.0	14.953787599630502	0.0
37	5.047777211305001E-4	0.0	0.0	15.082758307379345	0.0
38	5.047777211305001E-4	0.0	0.0	15.280126396341371	0.0
39	5.047777211305001E-4	0.0	0.0	15.651390410232853	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGTTG	35	1.2092278E-7	45.000004	1
ACATACG	60	0.0	45.000004	17
TCGTTAG	20	7.0276856E-4	45.0	1
GTACCGT	20	7.0276856E-4	45.0	37
ACCGGTC	20	7.0276856E-4	45.0	17
GATCAAT	20	7.0276856E-4	45.0	9
TGCGTTG	25	3.886163E-5	45.0	1
GTTGCGT	20	7.0276856E-4	45.0	39
CGTTTTT	11505	0.0	44.4133	1
AATGATC	235	0.0	42.12766	2
GATACCT	1000	0.0	41.85	5
TGATACC	1000	0.0	41.624996	4
CCGATGA	330	0.0	41.590908	18
ATACCTG	1040	0.0	40.67308	6
GAATCTG	1300	0.0	40.673077	1
TACGGCT	820	0.0	40.335365	7
ATGATCT	225	0.0	40.0	3
CGATGAA	350	0.0	39.857143	19
ACGGCTG	830	0.0	39.8494	8
AATCTGT	1330	0.0	39.755642	2
>>END_MODULE