FastQCFastQC Report
Sat 14 Jan 2017
SRR2933250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787900
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268793.4114735372509206No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC204212.591826373905318No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG187432.378855184668105No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC145211.8430003807589794No Hit
GAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT47590.6040106612514279Illumina PCR Primer Index 10 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTC40990.5202436857469221No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC32870.4171849219444092Illumina PCR Primer Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCT32560.4132504124888945No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCATTAGCT32220.408935144053814No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTA28760.3650209417438761No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT16710.21208275161822568No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGT13010.16512247747176037No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8260.10483563904048737No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA8190.10394720142150018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGA302.1646283E-645.0000049
CCGGTGA650.045.00000418
TCGTTAG253.8896855E-545.01
TACGCGG207.0319354E-445.02
CGTTTTT224250.044.4581951
TCAAGCG1200.043.12500417
TACGGCT18450.042.3170747
ACGGCTG18650.041.863278
ACCGGTG700.041.78571317
GATACCT22750.041.7362635
ACACGAC1250.041.426
GACACGA1250.041.425
AGACACG1250.041.424
CGGCTGT18900.041.3095259
GCTACGA603.6379788E-1241.25000410
CCGATGA5250.041.14285318
CTCGTTG556.002665E-1140.909091
CGATGAA5400.040.83333619
TGATACC23400.040.5769234
ATACCTG23450.040.4904066
Produced by FastQC (version 0.11.5)