##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933250.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	787900
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.488488386851124	31.0	28.0	33.0	25.0	34.0
2	28.756557938824724	31.0	28.0	33.0	16.0	34.0
3	28.744707450184034	31.0	28.0	34.0	16.0	34.0
4	33.173532174133776	35.0	32.0	37.0	28.0	37.0
5	34.239663662901386	35.0	33.0	37.0	30.0	37.0
6	33.73215890341414	35.0	33.0	37.0	28.0	37.0
7	33.65384693489021	35.0	33.0	37.0	29.0	37.0
8	33.60502982612007	35.0	33.0	37.0	28.0	37.0
9	34.7559639548166	37.0	34.0	39.0	28.0	39.0
10	34.69803020687905	37.0	33.0	39.0	27.0	39.0
11	35.08365147861404	37.0	34.0	39.0	28.0	39.0
12	35.16625587003427	37.0	34.0	39.0	28.0	39.0
13	35.341860642213476	37.0	34.0	39.0	30.0	39.0
14	36.089695392816346	38.0	34.0	40.0	28.0	41.0
15	36.42597156999619	38.0	34.0	40.0	30.0	41.0
16	36.34983119685239	38.0	34.0	40.0	30.0	41.0
17	35.63150399796928	38.0	33.0	40.0	27.0	41.0
18	35.366498286584594	38.0	33.0	39.0	27.0	40.0
19	34.69261327579642	37.0	33.0	38.0	27.0	40.0
20	34.00795405508313	35.0	33.0	38.0	27.0	40.0
21	34.323235182129714	35.0	33.0	38.0	27.0	40.0
22	34.79787663409062	35.0	33.0	39.0	29.0	40.0
23	35.130965858611496	35.0	33.0	39.0	30.0	40.0
24	34.55797436222871	35.0	33.0	39.0	27.0	40.0
25	33.525670770402336	35.0	33.0	38.0	24.0	40.0
26	33.546296484325424	35.0	32.0	38.0	24.0	40.0
27	34.37200659982231	35.0	33.0	38.0	27.0	40.0
28	34.10831196852392	35.0	33.0	39.0	25.0	40.0
29	34.13724711257774	35.0	33.0	39.0	24.0	40.0
30	33.40487879172484	35.0	33.0	39.0	22.0	40.0
31	33.33328468079706	35.0	33.0	39.0	21.0	40.0
32	32.72351567457799	35.0	32.0	39.0	18.0	40.0
33	32.072882345475314	35.0	31.0	39.0	14.0	40.0
34	31.376609975885266	35.0	30.0	39.0	10.0	40.0
35	30.95099251174007	35.0	27.0	40.0	8.0	41.0
36	30.2890354105851	35.0	23.0	40.0	7.0	41.0
37	30.215257012311206	35.0	23.0	40.0	7.0	40.0
38	29.9014925751999	35.0	22.0	39.0	7.0	40.0
39	29.469145830689175	35.0	21.0	39.0	7.0	40.0
40	29.33727249650971	35.0	21.0	39.0	7.0	40.0
41	28.85344713796167	35.0	18.0	39.0	7.0	40.0
42	29.04692219824851	35.0	18.0	39.0	7.0	40.0
43	28.988770148496002	35.0	18.0	39.0	7.0	40.0
44	29.025826881583956	35.0	18.0	39.0	7.0	40.0
45	29.10748318314507	35.0	20.0	39.0	7.0	40.0
46	28.88243812666582	35.0	19.0	39.0	7.0	40.0
47	28.82966493209798	35.0	20.0	39.0	7.0	40.0
48	28.68909633202183	35.0	19.0	38.0	7.0	40.0
49	28.894369843888818	35.0	20.0	39.0	7.0	40.0
50	28.894424419342556	35.0	20.0	39.0	7.0	40.0
51	27.818126665820536	33.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	28.0
10	44.0
11	53.0
12	70.0
13	107.0
14	133.0
15	229.0
16	384.0
17	820.0
18	1591.0
19	3003.0
20	4973.0
21	7127.0
22	10486.0
23	15203.0
24	22120.0
25	31566.0
26	40954.0
27	43405.0
28	39647.0
29	35691.0
30	35618.0
31	38027.0
32	43293.0
33	50378.0
34	58497.0
35	68980.0
36	76631.0
37	81697.0
38	66636.0
39	10501.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.821170199263868	2.3117146846046452	33.29559588780302	40.57151922832847
2	39.025637771290775	15.162457164614798	32.20345221474806	13.608452849346364
3	10.750222109404747	15.375936032491433	59.76139103947201	14.112450818631805
4	9.944536108643229	2.6186064221347887	71.84960020307145	15.587257266150525
5	19.69539281634725	2.950374412996573	60.86698819647164	16.487244574184544
6	12.997080847823328	12.880822439395862	63.35562888691458	10.766467825866227
7	50.951516689935275	1.0836400558446504	45.30574946059144	2.659093793628633
8	50.48813301180353	9.369336210178956	37.070313491559844	3.072217286457672
9	47.51846681050895	2.820408681304734	36.005584465033635	13.655540043152683
10	28.114989211828913	19.495367432415282	41.674831831450696	10.714811524305116
11	19.8327198883107	15.539916233024496	51.05889072217287	13.568473156491942
12	16.243812666582052	14.265769767737021	52.44777255996954	17.042645005711385
13	15.906714050006347	15.219824850869399	57.21944409188984	11.65401700723442
14	13.70871938063206	20.36578245970301	49.85175783728899	16.073740322375937
15	11.197867749714431	15.959766467825867	58.43647671024242	14.405889072217287
16	13.412361974869908	17.939840081228585	49.01294580530524	19.63485213859627
17	13.628252316283792	17.703388754918137	53.32250285569234	15.345856073105724
18	13.265642848077167	16.831577611372	52.85048864069044	17.052290899860388
19	13.398146972966114	18.92524432034522	49.43749206752126	18.239116639167406
20	15.3751745145323	18.4121081355502	54.00748825993147	12.20522908998604
21	15.228582307399416	21.53357025003173	50.51643609595126	12.721411346617591
22	13.54905444853408	14.969285442315014	52.743241528112705	18.738418581038204
23	13.814697296611245	20.348775225282395	52.356644244193426	13.479883233912934
24	15.339637009772813	17.066632821424037	50.07374032237594	17.51998984642721
25	12.679908617844903	24.508820916359944	48.00025383931971	14.81101662647544
26	13.457164614798833	16.35892879807082	51.607183652747814	18.576722934382538
27	15.592714811524305	18.067648178702882	48.318695265896686	18.020941743876126
28	11.305749460591445	18.747810635867495	52.44612260439142	17.500317299149636
29	15.131361847950247	15.52950882091636	49.14379997461607	20.195329356517323
30	13.106993273258027	20.35270973473791	49.93349409823581	16.606802893768243
31	15.167914709988578	20.051021703261835	46.83005457545374	17.95100901129585
32	16.908364005584463	20.031349155984262	48.34801370732326	14.712273131108008
33	14.115369970808478	19.434953674324156	45.739560857976905	20.71011549689047
34	16.512247747176037	23.589161061048355	42.43343063840589	17.465160553369717
35	17.01764183271989	21.658713034649065	40.6142911536997	20.709353978931336
36	15.6021068663536	24.592207132884884	38.04188348775225	21.763802513009264
37	15.852265515928417	23.216651859373016	42.648178702881076	18.28290392181749
38	17.41794643990354	24.748952912806192	39.475060286838435	18.35804036045183
39	18.205863688285316	25.451707069425055	38.940728518847564	17.40170072344206
40	20.282777002157633	22.654143926894278	40.58687650717096	16.47620256377713
41	16.806574438380505	27.56200025383932	35.39751237466683	20.23391293311334
42	18.794009392054832	23.587257266150527	40.43533443330372	17.183398908490926
43	18.88463002919152	22.182891229851503	36.290772940728516	22.641705800228458
44	19.159284173118415	23.41388501078817	36.24939713161569	21.177433684477727
45	17.077801751491307	23.228074628759995	36.768752379743624	22.925371240005077
46	20.869145830689174	24.47582180479756	35.79642086559208	18.85861149892118
47	15.249524051275543	22.134661759106486	43.84731564919406	18.768498540423913
48	18.039472014215	24.559208021322505	35.7682447011042	21.633075263358297
49	17.231628379235943	21.761517959131872	41.17984515801498	19.82700850361721
50	17.456656936159412	21.175783728899606	39.409188983373525	21.958370351567456
51	17.297626602360705	21.414900368067013	37.27110039345094	24.016372636121336
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	1239.5
2	2413.0
3	29602.5
4	56792.0
5	42618.5
6	28445.0
7	26994.5
8	25544.0
9	24609.5
10	23675.0
11	22604.0
12	21533.0
13	20143.5
14	18754.0
15	17415.0
16	16076.0
17	14845.0
18	13614.0
19	12484.0
20	11354.0
21	10840.5
22	10327.0
23	9756.0
24	9185.0
25	9113.5
26	9611.5
27	10181.0
28	10886.0
29	11591.0
30	12771.0
31	13951.0
32	15179.5
33	16408.0
34	18224.0
35	20040.0
36	21036.0
37	22032.0
38	23794.5
39	25557.0
40	26352.0
41	27147.0
42	28206.0
43	29265.0
44	31087.5
45	32910.0
46	71225.0
47	109540.0
48	78281.5
49	47023.0
50	42468.5
51	37914.0
52	33792.0
53	29670.0
54	27734.5
55	25799.0
56	23926.5
57	22054.0
58	19990.5
59	17927.0
60	15903.0
61	13879.0
62	11938.0
63	9997.0
64	8352.5
65	6708.0
66	5432.5
67	4157.0
68	3422.5
69	2688.0
70	2120.0
71	1552.0
72	1344.0
73	1136.0
74	992.5
75	688.0
76	527.0
77	409.5
78	292.0
79	218.5
80	145.0
81	122.0
82	99.0
83	60.0
84	21.0
85	18.0
86	15.0
87	8.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	787900.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.49732162654552
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.7943061350907	38.85010465718528
2	6.189800367252352	5.879978776949891
3	2.9562347443388286	4.212396973662898
4	2.1035006167194714	3.996425813358463
5	1.6061487833437065	3.8143882671280402
6	1.2161025424498013	3.465696815375876
7	0.9354938324799243	3.1103416008664055
8	0.7442592137454354	2.828025539902946
9	0.5748793541590808	2.4574706622859166
>10	1.7832483143424587	12.172273722042414
>50	0.05355825816308166	1.7703042464674419
>100	0.03678742647433359	3.1500297822789833
>500	0.002434462046072678	0.7978499448505311
>1k	0.0021639662631757137	3.1439913044182526
>5k	0.0	0.0
>10k+	0.0010819831315878568	10.35072189322676
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26879	3.4114735372509206	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	20421	2.591826373905318	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCG	18743	2.378855184668105	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	14521	1.8430003807589794	No Hit
GAACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	4759	0.6040106612514279	Illumina PCR Primer Index 10 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTC	4099	0.5202436857469221	No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	3287	0.4171849219444092	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCT	3256	0.4132504124888945	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCATTAGCT	3222	0.408935144053814	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCATTAGCTCGTA	2876	0.3650209417438761	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT	1671	0.21208275161822568	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGT	1301	0.16512247747176037	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	826	0.10483563904048737	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	819	0.10394720142150018	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.058890722172864576	0.0
2	0.0	0.0	0.0	0.558446503363371	0.0
3	0.0	0.0	0.0	0.6658205356009645	0.0
4	0.0	0.0	0.0	1.415915725345856	0.0
5	0.0	0.0	0.0	4.492955958878031	0.0
6	0.0	0.0	0.0	4.828531539535474	0.0
7	0.0	0.0	0.0	5.40741210813555	0.0
8	0.0	0.0	0.0	6.112450818631806	0.0
9	0.0	0.0	0.0	6.24419342556162	0.0
10	0.0	0.0	0.0	9.153826627744637	0.0
11	0.0	0.0	0.0	9.336210178956721	0.0
12	0.0	0.0	0.0	11.65998223124762	0.0
13	0.0	0.0	0.0	11.861784490417566	0.0
14	0.0	0.0	0.0	12.019418707957863	0.0
15	0.0	0.0	0.0	12.532935651732453	0.0
16	0.0	0.0	0.0	12.800355375047594	0.0
17	0.0	0.0	0.0	12.934890214494224	0.0
18	0.0	0.0	0.0	13.04873714938444	0.0
19	0.0	0.0	0.0	13.666328214240385	0.0
20	0.0	0.0	0.0	13.812285823073994	0.0
21	0.0	0.0	0.0	13.924863561365656	0.0
22	0.0	0.0	0.0	14.149257519989847	0.0
23	0.0	0.0	0.0	14.27262342936921	0.0
24	0.0	0.0	0.0	14.382535854803908	0.0
25	0.0	0.0	0.0	14.467318187587257	0.0
26	0.0	0.0	0.0	14.579134407919787	0.0
27	0.0	0.0	0.0	14.73549942886153	0.0
28	0.0	0.0	0.0	14.83297372763041	0.0
29	0.0	0.0	0.0	14.947962939459321	0.0
30	0.0	0.0	0.0	15.073486483056225	0.0
31	0.0	0.0	0.0	15.183906587130346	0.0
32	0.0	0.0	0.0	15.30079959385709	0.0
33	0.0	0.0	0.0	15.417058002284554	0.0
34	0.0	0.0	0.0	15.563904048737148	0.0
35	0.0	0.0	0.0	15.713796167026272	0.0
36	0.0	0.0	0.0	15.851503997969285	0.0
37	0.0	0.0	0.0	16.002665312856962	0.0
38	0.0	0.0	0.0	16.153065109785505	0.0
39	0.0	0.0	0.0	16.369336210178957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACGA	30	2.1646283E-6	45.000004	9
CCGGTGA	65	0.0	45.000004	18
TCGTTAG	25	3.8896855E-5	45.0	1
TACGCGG	20	7.0319354E-4	45.0	2
CGTTTTT	22425	0.0	44.458195	1
TCAAGCG	120	0.0	43.125004	17
TACGGCT	1845	0.0	42.317074	7
ACGGCTG	1865	0.0	41.86327	8
ACCGGTG	70	0.0	41.785713	17
GATACCT	2275	0.0	41.736263	5
ACACGAC	125	0.0	41.4	26
GACACGA	125	0.0	41.4	25
AGACACG	125	0.0	41.4	24
CGGCTGT	1890	0.0	41.309525	9
GCTACGA	60	3.6379788E-12	41.250004	10
CCGATGA	525	0.0	41.142853	18
CTCGTTG	55	6.002665E-11	40.90909	1
CGATGAA	540	0.0	40.833336	19
TGATACC	2340	0.0	40.576923	4
ATACCTG	2345	0.0	40.490406	6
>>END_MODULE