##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933248.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	273841
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.798488904145106	31.0	28.0	33.0	25.0	34.0
2	28.9122848660354	31.0	28.0	33.0	16.0	34.0
3	28.884250349655456	31.0	28.0	34.0	16.0	34.0
4	33.28268593819041	35.0	32.0	37.0	28.0	37.0
5	34.47166786565927	35.0	35.0	37.0	32.0	37.0
6	34.06246690597828	35.0	33.0	37.0	28.0	37.0
7	33.82335369794881	35.0	33.0	37.0	29.0	37.0
8	33.8430914289679	35.0	33.0	37.0	28.0	37.0
9	35.12912237393232	37.0	34.0	39.0	30.0	39.0
10	35.048531812256016	37.0	34.0	39.0	28.0	39.0
11	35.32648142535267	37.0	34.0	39.0	30.0	39.0
12	35.39518552736807	37.0	34.0	39.0	30.0	39.0
13	35.55783830763107	37.0	34.0	39.0	30.0	39.0
14	36.45509985721641	38.0	35.0	40.0	30.0	41.0
15	36.72629372519089	39.0	35.0	41.0	30.0	41.0
16	36.69649906332507	38.0	34.0	41.0	31.0	41.0
17	36.081952665963094	38.0	34.0	40.0	29.0	41.0
18	35.771250470163345	38.0	34.0	39.0	29.0	40.0
19	35.05422489692924	37.0	34.0	38.0	27.0	40.0
20	34.38279877739272	35.0	33.0	38.0	27.0	40.0
21	34.69384058632564	35.0	34.0	38.0	29.0	40.0
22	35.17925730624705	35.0	33.0	39.0	30.0	40.0
23	35.51095708823733	35.0	34.0	39.0	30.0	40.0
24	35.065282408404876	35.0	34.0	39.0	30.0	40.0
25	34.08686427525462	35.0	33.0	38.0	25.0	40.0
26	34.08985506187897	35.0	33.0	38.0	25.0	40.0
27	34.872991991703216	35.0	34.0	39.0	30.0	40.0
28	34.60650888654365	35.0	33.0	39.0	27.0	40.0
29	34.63656282295201	35.0	33.0	39.0	25.0	41.0
30	33.874865341566824	35.0	33.0	39.0	23.0	40.0
31	33.753269232876015	35.0	33.0	39.0	23.0	40.0
32	33.0851698613429	35.0	33.0	39.0	18.0	40.0
33	32.40674698091228	35.0	32.0	39.0	15.0	41.0
34	31.715962912785155	35.0	30.0	40.0	10.0	41.0
35	31.199845895976132	35.0	28.0	40.0	8.0	41.0
36	30.63761452813859	35.0	24.0	40.0	7.0	41.0
37	30.465211564374947	35.0	23.0	40.0	7.0	41.0
38	30.252226657074726	35.0	23.0	40.0	7.0	41.0
39	29.863738446762902	35.0	22.0	39.0	7.0	40.0
40	29.587600103709818	35.0	21.0	39.0	7.0	41.0
41	29.182014380607725	35.0	20.0	39.0	7.0	40.0
42	29.288945044752246	35.0	20.0	39.0	7.0	40.0
43	29.244729605866176	35.0	20.0	39.0	7.0	40.0
44	29.3085476608689	35.0	20.0	39.0	7.0	40.0
45	29.423629040209466	35.0	20.0	39.0	7.0	40.0
46	29.178764319440845	35.0	20.0	39.0	7.0	40.0
47	28.99482911616595	35.0	20.0	39.0	7.0	40.0
48	28.927549198257385	35.0	20.0	39.0	7.0	40.0
49	29.04628233171804	35.0	20.0	39.0	7.0	40.0
50	29.020942809878726	35.0	20.0	39.0	7.0	40.0
51	27.889713373819113	34.0	18.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	10.0
10	15.0
11	11.0
12	13.0
13	27.0
14	29.0
15	67.0
16	106.0
17	216.0
18	413.0
19	739.0
20	1169.0
21	1827.0
22	2880.0
23	4408.0
24	6928.0
25	10790.0
26	14310.0
27	14927.0
28	13582.0
29	12286.0
30	11985.0
31	12983.0
32	14332.0
33	17095.0
34	19750.0
35	24059.0
36	27103.0
37	30256.0
38	26787.0
39	4732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.499983567106458	2.228665539491895	32.53055605259987	40.74079484080178
2	39.63102676370595	14.89952198538568	31.517194284274453	13.952256966633922
3	11.511424512764707	15.195314069113097	59.30083515616727	13.992426261954929
4	10.108785755237529	2.6822134012072696	71.33007840316095	15.878922440394245
5	20.23838650895958	3.0901143364215002	60.54827436359055	16.12322479102837
6	12.975777914921432	13.061594136743585	63.079305144226026	10.883322804108953
7	51.189924080031844	0.978670104184545	45.432933709707456	2.3984721060761536
8	51.0686858432448	9.514645359898626	36.78265855003451	2.634010246822061
9	48.14107456516738	2.500356046026709	36.060341585080394	13.29822780372552
10	29.07745735664126	18.302956825311036	41.24729313725848	11.372292680789217
11	22.067915323125465	15.224162926661823	50.01369407795034	12.69422767226237
12	17.107372526393053	14.50075043547168	51.44226028973017	16.9496167484051
13	16.080864443235306	15.118992408003184	57.059753652667055	11.740389496094448
14	14.429906405541901	19.599329537943554	50.51581026946293	15.45495378705161
15	11.782749843887512	15.19020161334497	58.36342987353975	14.663618669227763
16	14.431732282601947	17.02301700621894	48.996315380092824	19.548935331086287
17	14.30026913427865	17.34619724584704	53.52631636606644	14.827217253807866
18	14.953567946363036	15.92383901607137	52.08752524275035	17.03506779481524
19	14.319258255703126	18.673244693088325	49.51449928973382	17.492997761474722
20	16.43618011912022	17.96809097249864	53.66508302262992	11.93064588575122
21	15.966564539276442	22.19974364686077	49.9045066297596	11.929185184103185
22	14.628927005086895	14.329117991827376	53.05304903210257	17.98890597098316
23	14.299173608042622	21.095088025533066	51.49375002282346	13.11198834360085
24	15.640097720940254	16.741466763559878	50.19956836266301	17.418867152836864
25	13.680201284687099	24.394082697623805	47.47864636778276	14.447069649906332
26	13.713432247179933	16.576772652743745	51.80743570173934	17.90235939833699
27	16.061144970986813	18.099554120821935	48.50807585423658	17.331225053954665
28	11.876599924773865	18.839764680964503	53.076055083059146	16.207580311202484
29	15.948305768675983	16.692898433762657	50.636683330837975	16.72211246672339
30	14.123524234866219	22.19937847144876	50.69547657217144	12.981620721513579
31	16.73891053567581	17.067933581896064	48.3853769158015	17.80777896662662
32	18.560040315365487	20.33734904561406	45.258745038179086	15.843865600841363
33	17.951292903546218	19.19142860272932	41.81112397340062	21.04615452032384
34	17.739125989168897	20.60684849967682	44.59960341950256	17.054422091651723
35	17.996574654635353	25.751074528649838	38.96421646137722	17.288134355337586
36	17.47473899087427	25.13831018729847	41.51971399461731	15.867236827209949
37	17.045657881763503	25.54986287663279	38.37518852180645	19.029290719797253
38	21.90029980901326	23.63597854229279	37.97203486694834	16.491686781745614
39	19.41747218276299	22.76430483382693	35.78719037689754	22.03103260651254
40	24.792854247537804	22.687252821892997	36.91156547047374	15.608327460095456
41	18.099188945409928	27.456808878144617	34.877903600994735	19.56609857545072
42	20.35159088668242	23.40153592778291	38.943036287480695	17.30383689805398
43	20.00467424527372	24.19615762431484	36.076774478620806	19.722393651790636
44	21.181269422767226	23.664097049017496	35.5224382031909	19.632195325024377
45	18.6962507440449	22.012773835912082	36.81479398629131	22.4761814337517
46	21.654901932143105	24.493410409690295	34.3779784619542	19.4737091962124
47	17.23408839436023	22.72888281886204	41.842529058833414	18.19449972794432
48	19.160753868120555	24.666138379570626	35.11891937292078	21.054188379388037
49	18.562596543249548	21.993784714487603	39.1110169770049	20.33260176525794
50	18.810915823415776	22.5598066031018	37.44070464247501	21.18857293100741
51	18.487005232963654	21.758976924565715	35.94385062864947	23.81016721382116
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	40.0
1	540.5
2	1041.0
3	10695.0
4	20349.0
5	14882.5
6	9416.0
7	8768.5
8	8121.0
9	7887.0
10	7653.0
11	7409.0
12	7165.0
13	6827.5
14	6490.0
15	6001.0
16	5512.0
17	5179.5
18	4847.0
19	4466.0
20	4085.0
21	3775.5
22	3466.0
23	3333.5
24	3201.0
25	3284.0
26	3503.5
27	3640.0
28	3857.0
29	4074.0
30	4582.5
31	5091.0
32	5399.0
33	5707.0
34	6185.0
35	6663.0
36	6913.0
37	7163.0
38	7705.5
39	8248.0
40	8369.5
41	8491.0
42	8811.0
43	9131.0
44	9808.5
45	10486.0
46	23855.5
47	37225.0
48	26189.5
49	15154.0
50	13945.5
51	12737.0
52	11633.0
53	10529.0
54	10225.0
55	9921.0
56	9210.0
57	8499.0
58	7745.5
59	6992.0
60	6202.0
61	5412.0
62	4835.5
63	4259.0
64	3675.5
65	3092.0
66	2609.0
67	2126.0
68	1819.5
69	1513.0
70	1243.0
71	973.0
72	825.5
73	678.0
74	634.5
75	460.0
76	329.0
77	258.5
78	188.0
79	143.0
80	98.0
81	73.5
82	49.0
83	33.5
84	18.0
85	14.0
86	10.0
87	5.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	273841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.59860191858734
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.89958730083231	46.59703124318644
2	9.736235339693785	11.215870870334086
3	4.446593993355119	7.683527919505294
4	2.1774708433836407	5.016771051895399
5	1.0915904648338723	3.1437042321045965
6	0.5529107431922624	1.9108131476184842
7	0.3095463596895828	1.2480606282975693
8	0.1708886304451153	0.7874356957936627
9	0.09403241904877979	0.4874522285009212
>10	0.4456924173165938	4.726453712041157
>50	0.048595873761698494	1.9438696129219322
>100	0.019822001139640173	1.953445325300858
>500	0.0019182581748038879	0.9932960109985697
>1k	0.002557677566405184	1.8193853519958971
>5k	0.002557677566405184	10.472882969505127
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9652	3.5246730767123986	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG	6875	2.5105809575629654	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTC	6102	2.2283003640798857	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	5807	2.1205736175371843	No Hit
GAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT	1433	0.5232963654091243	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTATCGCGAT	1253	0.45756479124747573	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTC	1241	0.45318268630336583	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	1013	0.36992269236527764	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTT	938	0.3425345364645908	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCT	932	0.34034348399253583	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTA	827	0.3020000657315741	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTATCGCGATCGT	460	0.16798068952421297	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	351	0.12817656961521465	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATG	300	0.10955262360274759	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03396131331685175	0.0
2	0.0	0.0	0.0	0.44770505512322845	0.0
3	0.0	0.0	0.0	0.5349819785934173	0.0
4	0.0	0.0	0.0	1.167100616781271	0.0
5	0.0	0.0	0.0	3.8047626177234233	0.0
6	0.0	0.0	0.0	4.272917495919165	0.0
7	0.0	0.0	0.0	4.734134041286732	0.0
8	0.0	0.0	0.0	5.332656541569743	0.0
9	0.0	0.0	0.0	5.461198286596967	0.0
10	0.0	0.0	0.0	8.4848506980328	0.0
11	0.0	0.0	0.0	8.6323815644845	0.0
12	0.0	0.0	0.0	11.155378486055778	0.0
13	0.0	0.0	0.0	11.375944434909309	0.0
14	0.0	0.0	0.0	11.511789688176716	0.0
15	0.0	0.0	0.0	11.906909483970626	0.0
16	0.0	0.0	0.0	12.154498413312835	0.0
17	0.0	0.0	0.0	12.283405333752068	0.0
18	0.0	0.0	0.0	12.36922155557422	0.0
19	0.0	0.0	0.0	12.999879492114037	0.0
20	0.0	0.0	0.0	13.137915797853498	0.0
21	0.0	0.0	0.0	13.21131605566734	0.0
22	0.0	0.0	0.0	13.377470868131507	0.0
23	0.0	0.0	0.0	13.45452288006544	0.0
24	0.0	0.0	0.0	13.539608751063573	0.0
25	0.0	0.0	0.0	13.607896553109287	0.0
26	0.0	0.0	0.0	13.702476984819658	0.0
27	0.0	0.0	0.0	13.82736697572679	0.0
28	0.0	0.0	0.0	13.893828900712457	0.0
29	0.0	0.0	0.0	13.98366205206671	0.0
30	0.0	0.0	0.0	14.084815641193247	0.0
31	0.0	0.0	0.0	14.168805985955354	0.0
32	0.0	0.0	0.0	14.286392468622303	0.0
33	0.0	0.0	0.0	14.381703251156694	0.0
34	0.0	0.0	0.0	14.51791367983611	0.0
35	0.0	0.0	0.0	14.63878674121114	0.0
36	0.0	0.0	0.0	14.73081094503745	0.0
37	0.0	0.0	0.0	14.85168400641248	0.0
38	0.0	0.0	0.0	14.993737241684043	0.0
39	0.0	0.0	0.0	15.193488192053053	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	20	7.0238643E-4	45.0	24
ACCGGGC	20	7.0238643E-4	45.0	30
GGCACTC	20	7.0238643E-4	45.0	9
TAACGTC	25	3.882998E-5	45.0	32
TCGATCA	20	7.0238643E-4	45.0	17
CCTCGAT	20	7.0238643E-4	45.0	15
GGGCGAA	20	7.0238643E-4	45.0	7
TTGCGCG	25	3.882998E-5	45.0	1
TAGGGCG	25	3.882998E-5	45.0	5
TAGGGAC	25	3.882998E-5	45.0	5
CTCGATC	20	7.0238643E-4	45.0	16
CACGACC	65	0.0	44.999996	27
AAACACG	60	0.0	44.999996	40
CGTTTTT	7505	0.0	44.430378	1
GATACCT	890	0.0	43.230335	5
TGATACC	905	0.0	42.762432	4
ATACCTG	915	0.0	42.049183	6
GCGAGAC	75	0.0	42.0	21
ACACGAC	70	0.0	41.785717	26
TACGGCT	700	0.0	41.785713	7
>>END_MODULE