##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933247.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	249736
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.127967133292756	31.0	28.0	31.0	23.0	34.0
2	29.506330685203576	31.0	30.0	33.0	16.0	34.0
3	29.655456161706763	31.0	30.0	34.0	16.0	34.0
4	32.497209052759715	35.0	32.0	37.0	19.0	37.0
5	33.91166672005638	35.0	33.0	37.0	30.0	37.0
6	33.34783931832015	35.0	35.0	37.0	27.0	37.0
7	32.29159192106865	35.0	32.0	35.0	25.0	37.0
8	33.4865257712144	35.0	33.0	37.0	28.0	37.0
9	33.3927507447865	35.0	32.0	37.0	26.0	39.0
10	31.794815324983183	35.0	27.0	39.0	15.0	39.0
11	32.69120190921613	37.0	30.0	39.0	17.0	39.0
12	33.45246179966044	37.0	31.0	39.0	23.0	39.0
13	33.67396770990165	37.0	32.0	39.0	25.0	39.0
14	33.90603677483423	37.0	32.0	40.0	21.0	41.0
15	34.54417464842874	37.0	32.0	40.0	25.0	41.0
16	34.75584617355928	37.0	32.0	40.0	25.0	41.0
17	32.87863984367492	36.0	30.0	40.0	18.0	41.0
18	33.517330300797646	36.0	31.0	39.0	24.0	39.0
19	33.25567799596374	37.0	32.0	37.0	23.0	39.0
20	32.96824646827049	35.0	31.0	37.0	25.0	39.0
21	33.16058157414229	35.0	32.0	37.0	25.0	39.0
22	33.33593074286446	35.0	31.0	38.0	26.0	39.0
23	34.44458147804081	35.0	33.0	38.0	28.0	40.0
24	32.693852708460135	35.0	31.0	37.0	23.0	39.0
25	31.724228785597592	35.0	30.0	37.0	18.0	39.0
26	30.95301438318865	33.0	25.0	37.0	20.0	39.0
27	32.83461735592786	35.0	31.0	38.0	23.0	39.0
28	32.88642005958292	35.0	31.0	38.0	23.0	39.0
29	33.155215907998844	35.0	31.0	38.0	22.0	40.0
30	32.01439119710414	35.0	30.0	38.0	20.0	40.0
31	32.36560207579203	35.0	30.0	38.0	21.0	40.0
32	32.17465643719768	35.0	31.0	38.0	20.0	40.0
33	32.01512797514175	35.0	31.0	38.0	16.0	40.0
34	31.025038440593267	35.0	30.0	38.0	12.0	40.0
35	30.332567190953647	35.0	27.0	38.0	9.0	40.0
36	29.724224781369127	35.0	24.0	38.0	7.0	40.0
37	29.757167568952813	35.0	24.0	38.0	7.0	40.0
38	29.76598888426178	35.0	23.0	38.0	7.0	40.0
39	29.317855655572284	35.0	23.0	38.0	7.0	40.0
40	29.077113431783964	34.0	22.0	38.0	7.0	40.0
41	28.64809238555915	34.0	21.0	38.0	7.0	40.0
42	29.014235032193998	34.0	22.0	38.0	7.0	40.0
43	29.011544190665344	34.0	21.0	38.0	7.0	40.0
44	28.940557228433224	34.0	21.0	38.0	7.0	40.0
45	29.18352580324823	35.0	22.0	38.0	7.0	40.0
46	28.92217381554922	34.0	21.0	38.0	7.0	40.0
47	28.89470480827754	34.0	20.0	38.0	7.0	40.0
48	28.71901928436429	34.0	21.0	38.0	7.0	40.0
49	28.419390876765863	34.0	20.0	37.0	7.0	40.0
50	28.608955056539706	34.0	20.0	38.0	7.0	40.0
51	27.116843386616267	32.0	19.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	12.0
10	12.0
11	11.0
12	24.0
13	24.0
14	36.0
15	78.0
16	181.0
17	334.0
18	581.0
19	1028.0
20	1639.0
21	2410.0
22	3483.0
23	5114.0
24	7388.0
25	10498.0
26	13513.0
27	14411.0
28	13595.0
29	13560.0
30	14357.0
31	16489.0
32	19071.0
33	21859.0
34	25108.0
35	28319.0
36	21845.0
37	12025.0
38	2685.0
39	43.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.167040394656755	2.3080372873754684	36.20343082294903	42.32149149501873
2	42.10045808373643	9.060768171188775	35.08304769836948	13.755726046705322
3	12.435131498862798	9.067575359579717	64.51572860941154	13.981564532145946
4	11.27951116378896	2.788945126053112	69.15062305794919	16.780920652208735
5	16.55868597238684	3.271054233270333	63.657222026459934	16.513037767882885
6	13.852628375564596	8.200659896851075	65.79147579844316	12.155235929141172
7	54.02144664765993	1.0479065893583626	42.34751898004293	2.5831277829387833
8	56.055995130858186	5.5038120254989265	35.81902489028414	2.621167953358747
9	52.430566678412404	2.44858570650607	35.84425152961527	9.276596085466252
10	28.42201364641061	19.315196847871352	41.09900054457507	11.163788961142968
11	20.389931767946955	16.38089822852933	50.49892686677131	12.730243136752412
12	17.11687542044399	15.383845340679759	52.124643623666586	15.37463561520966
13	15.630505814139731	15.933225486113336	55.92705897427683	12.509209725470097
14	14.279879552807765	17.26543229650511	52.534676618509145	15.92001153217798
15	12.45475221834257	15.75183393663709	56.991783323189296	14.801630521831052
16	15.16321235224397	15.868757407822661	50.574206361918186	18.393823878015183
17	14.826056315469135	16.306820001922027	53.34833584264984	15.518787839958998
18	15.222474933529808	15.610484671813435	52.613559919274756	16.553480475382003
19	15.13558317583368	17.96216804946023	50.17538520677836	16.726863567927733
20	16.539065252907072	18.157173975718358	52.61756414774001	12.686196623634558
21	16.62395489637057	19.635935547938622	50.316334048755486	13.423775506935323
22	15.434699042188551	15.485152320850817	51.98970112438735	17.090447512573277
23	14.960598391901847	19.77007720152481	51.06992984591729	14.199394560656053
24	15.196847871352148	17.179741807348563	51.09956113656021	16.523849184739085
25	14.685107473492007	20.667424800589423	48.69221898324631	15.955248742672262
26	14.287487586891759	17.753147323573693	51.15441906653426	16.80494602300029
27	14.831662235320497	18.007415831117658	49.764551366242756	17.39637056731909
28	12.6605695614569	18.449883076528813	52.613559919274756	16.275987442739535
29	15.296152737290578	16.957507127526668	50.70594547842522	17.040394656757535
30	14.711134958516192	19.77408142999007	51.06992984591729	14.444853765576449
31	15.714194189063651	17.85485472659128	49.56033571451452	16.870615369830542
32	17.23099593170388	19.332014607425442	47.45891661594644	15.97807284492424
33	16.6059358682769	19.137008681167313	44.97709581317872	19.27995963737707
34	16.98673799532306	20.758320786750808	45.731892878880096	16.52304833904603
35	16.74968767017971	24.78337124002947	41.44216292404779	17.024778165743022
36	17.385959573309414	23.795127654803473	42.4227984751898	16.396114296697313
37	16.957106704680143	24.430598712240126	40.01906012749463	18.593234455585097
38	20.36951020277413	22.896178364352757	40.464730755678	16.269580677195115
39	18.81426786686741	22.495355094980297	38.67604190024666	20.01433513790563
40	22.487747060896307	22.6034692635423	38.93191209917673	15.976871576384664
41	17.844844155428135	25.486914181375532	38.24718903161739	18.421052631578945
42	19.092161322356407	23.01630521831054	40.214866899445816	17.67666655988724
43	18.941201909216133	23.222122561424865	39.025611045263794	18.811064484095205
44	19.820130057340553	23.302607553576575	37.849569145017135	19.027693244065734
45	18.125940993689333	22.27952718070282	38.61557805042125	20.9789537751866
46	19.590687766281192	23.97571835858667	37.12920844411699	19.304385431015152
47	17.23860396578787	22.376829932408622	42.02477816574303	18.359787936060478
48	18.125140147996284	23.60212384277797	37.838757728160935	20.433978281064803
49	18.01902809366691	22.338789761988657	39.33513790562834	20.307044238716085
50	18.08910209180895	22.307957202806165	39.00999455424929	20.5929461511356
51	18.17559342665855	21.45665823109203	38.206345901271746	22.161402440977675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	239.5
2	421.0
3	12587.5
4	24754.0
5	16545.0
6	8336.0
7	7762.0
8	7188.0
9	7126.5
10	7065.0
11	6744.5
12	6424.0
13	6077.0
14	5730.0
15	5255.0
16	4780.0
17	4427.5
18	4075.0
19	3719.5
20	3364.0
21	3134.0
22	2904.0
23	2784.5
24	2665.0
25	2677.0
26	2835.5
27	2982.0
28	3255.0
29	3528.0
30	3970.5
31	4413.0
32	4827.0
33	5241.0
34	5710.0
35	6179.0
36	6556.0
37	6933.0
38	7225.0
39	7517.0
40	8009.5
41	8502.0
42	8706.5
43	8911.0
44	9592.0
45	10273.0
46	16076.0
47	21879.0
48	18559.0
49	15239.0
50	14133.0
51	13027.0
52	11898.5
53	10770.0
54	9891.5
55	9013.0
56	8314.5
57	7616.0
58	6994.5
59	6373.0
60	5875.0
61	5377.0
62	4810.0
63	4243.0
64	3748.0
65	3253.0
66	2836.5
67	2420.0
68	2093.0
69	1766.0
70	1486.5
71	1207.0
72	1037.0
73	867.0
74	788.0
75	583.5
76	458.0
77	387.5
78	317.0
79	231.0
80	145.0
81	112.0
82	79.0
83	52.5
84	26.0
85	18.0
86	10.0
87	8.0
88	6.0
89	4.0
90	2.0
91	2.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	249736.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.10036978166164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.82224252861866	44.21956952875323
2	10.063661846822392	11.29150301928672
3	4.715695597573293	7.936568004048468
4	2.6589659776713592	5.966758983368828
5	1.4751244865182644	4.137751458382919
6	0.7816220835487008	2.630957274995697
7	0.467940358328188	1.8376138996582219
8	0.2630851545572193	1.1807339563780486
9	0.17296839645902454	0.8733231901703099
>10	0.5001414996869333	4.917033848870383
>50	0.05188760601424555	2.0036790571533913
>100	0.021619835839268978	2.2009743315462194
>500	0.002161983583926898	0.773413647363688
>1k	0.002161983583926898	3.3414865632309887
>5k	0.0	0.0
>10k+	7.206611946422993E-4	6.68863323679292
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16544	6.624595572925009	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCG	3383	1.354630489797226	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGC	2543	1.0182752987154435	No Hit
GAATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTC	2339	0.9365890380241535	No Hit
GAACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCT	776	0.31072812890412277	No Hit
GCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTTCTGC	579	0.2318448281385143	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTATCGCGAT	558	0.22343594836146974	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTC	427	0.1709805554665727	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCTT	331	0.1325399622000833	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTA	311	0.12453150526956465	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTATCGCGATCGTATGCCGTCT	273	0.10931543710157927	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTATCGCGATCGTATGCCG	272	0.10891501425505333	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	257	0.10290867155716436	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.041243553192170934	0.0
2	0.0	0.0	0.0	0.3840055098183682	0.0
3	0.0	0.0	0.0	0.5025306723900439	0.0
4	0.0	0.0	0.0	1.0110676874779767	0.0
5	0.0	0.0	0.0	2.3084377102219946	0.0
6	0.0	0.0	0.0	2.709260979594452	0.0
7	0.0	0.0	0.0	3.0492199762949674	0.0
8	0.0	0.0	0.0	3.4760707306916103	0.0
9	0.0	0.0	0.0	3.705112598904443	0.0
10	0.0	0.0	0.0	5.661578627030144	0.0
11	0.0	0.0	0.0	6.059999359323445	0.0
12	0.0	0.0	0.0	7.510731332286895	0.0
13	0.0	0.0	0.0	7.701332607233239	0.0
14	0.0	0.0	0.0	7.81825607841881	0.0
15	0.0	0.0	0.0	8.078530928660665	0.0
16	0.0	0.0	0.0	8.243104718582824	0.0
17	0.0	0.0	0.0	8.381651023480796	0.0
18	0.0	0.0	0.0	8.518996059839191	0.0
19	0.0	0.0	0.0	8.928228208988692	0.0
20	0.0	0.0	0.0	9.074782970817182	0.0
21	0.0	0.0	0.0	9.238155492199763	0.0
22	0.0	0.0	0.0	9.446375372393247	0.0
23	0.0	0.0	0.0	9.63417368741391	0.0
24	0.0	0.0	0.0	9.768315341000097	0.0
25	0.0	0.0	0.0	9.888442194957875	0.0
26	0.0	0.0	0.0	10.015376237306596	0.0
27	0.0	0.0	0.0	10.145113239580997	0.0
28	0.0	0.0	0.0	10.272447704776244	0.0
29	0.0	0.0	0.0	10.404987666976327	0.0
30	0.0	0.0	0.0	10.553144120190922	0.0
31	0.0	0.0	0.0	10.66446167152513	0.0
32	0.0	0.0	0.0	10.791796136720377	0.0
33	0.0	0.0	0.0	10.927939904539194	0.0
34	0.0	0.0	0.0	11.06208155812538	0.0
35	0.0	0.0	0.0	11.21864689111702	0.0
36	0.0	0.0	0.0	11.352788544703207	0.0
37	0.0	0.0	0.0	11.503347534996957	0.0
38	0.0	0.0	0.0	11.666720056379537	0.0
39	0.0	0.0	0.0	11.898965307364577	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACATACG	30	2.1586548E-6	45.000004	17
CTTGTCG	20	7.0226716E-4	45.0	1
GGCACGT	20	7.0226716E-4	45.0	11
CATGATA	20	7.0226716E-4	45.0	2
TCGATCA	20	7.0226716E-4	45.0	17
CCCACGA	20	7.0226716E-4	45.0	39
AGGCACG	20	7.0226716E-4	45.0	10
CCCCACG	20	7.0226716E-4	45.0	38
CTCGATC	20	7.0226716E-4	45.0	16
CGGTAGG	25	3.8820097E-5	45.0	31
TGTACGG	20	7.0226716E-4	45.0	2
CGCCCAA	20	7.0226716E-4	45.0	38
ACGACGA	20	7.0226716E-4	45.0	34
CGTTTTT	7550	0.0	44.642384	1
CGACCAC	195	0.0	43.846153	12
TACGGCT	315	0.0	42.857143	7
GATACCT	450	0.0	42.0	5
GCGACCA	205	0.0	41.70732	11
TGATACC	460	0.0	41.086956	4
ATGATAC	1150	0.0	41.086956	3
>>END_MODULE