##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933246.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620512
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.322893030271775	31.0	28.0	31.0	25.0	34.0
2	28.377101490382135	31.0	26.0	33.0	16.0	34.0
3	28.355126089422928	31.0	25.0	33.0	16.0	34.0
4	33.043983033366	35.0	32.0	37.0	28.0	37.0
5	34.20201220927234	35.0	33.0	37.0	30.0	37.0
6	33.568353230880305	35.0	33.0	37.0	28.0	37.0
7	33.68930496106441	35.0	33.0	37.0	29.0	37.0
8	33.477181746686604	35.0	33.0	37.0	28.0	37.0
9	34.73634353566087	37.0	34.0	39.0	28.0	39.0
10	34.78148367799495	37.0	33.0	39.0	28.0	39.0
11	35.17189353308236	37.0	34.0	39.0	29.0	39.0
12	35.287420710638955	37.0	34.0	39.0	30.0	39.0
13	35.380538974266415	37.0	34.0	39.0	30.0	39.0
14	36.15046606673199	38.0	34.0	40.0	29.0	41.0
15	36.390617747924296	38.0	34.0	40.0	30.0	41.0
16	36.41129422154608	38.0	34.0	40.0	30.0	41.0
17	35.64315919756588	38.0	33.0	40.0	27.0	41.0
18	35.39935085864576	38.0	34.0	39.0	27.0	40.0
19	34.689074506214226	37.0	33.0	38.0	27.0	40.0
20	33.758252862152546	35.0	32.0	37.0	27.0	40.0
21	34.218638801505854	35.0	33.0	38.0	27.0	40.0
22	34.698777783507815	35.0	33.0	39.0	29.0	40.0
23	34.97130917693775	35.0	33.0	39.0	30.0	40.0
24	34.46174771801351	35.0	33.0	38.0	27.0	40.0
25	33.42734548244031	35.0	33.0	38.0	24.0	40.0
26	33.4159984013202	35.0	32.0	38.0	24.0	40.0
27	34.252746119333715	35.0	33.0	38.0	26.0	40.0
28	33.86152080862256	35.0	33.0	39.0	24.0	40.0
29	33.86897594244753	35.0	33.0	39.0	24.0	40.0
30	33.20798791965345	35.0	33.0	39.0	21.0	40.0
31	33.26048488989738	35.0	33.0	39.0	21.0	40.0
32	32.49973892527461	35.0	31.0	38.0	17.0	40.0
33	31.701733407250785	35.0	30.0	39.0	12.0	40.0
34	31.07098331700273	35.0	27.0	39.0	8.0	40.0
35	30.438062438760248	35.0	24.0	39.0	7.0	40.0
36	29.96323681089165	35.0	22.0	40.0	7.0	40.0
37	29.75037871950905	35.0	21.0	40.0	7.0	40.0
38	29.603490343458304	35.0	21.0	39.0	7.0	40.0
39	29.06820980093858	35.0	18.0	39.0	7.0	40.0
40	28.83832705894487	35.0	18.0	39.0	7.0	40.0
41	28.39873523799701	35.0	18.0	39.0	7.0	40.0
42	28.55869024289619	35.0	18.0	39.0	7.0	40.0
43	28.573318485379815	35.0	18.0	39.0	7.0	40.0
44	28.617494907431283	35.0	17.0	39.0	7.0	40.0
45	28.758863648084162	35.0	18.0	39.0	7.0	40.0
46	28.458126514878035	35.0	17.0	39.0	7.0	40.0
47	28.38371699499768	35.0	17.0	39.0	7.0	40.0
48	28.24377449590016	35.0	17.0	39.0	7.0	40.0
49	28.417497163632614	35.0	17.0	39.0	7.0	40.0
50	28.375383554226186	35.0	17.0	39.0	7.0	40.0
51	27.426088455984736	33.0	15.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	24.0
10	33.0
11	34.0
12	67.0
13	71.0
14	125.0
15	186.0
16	322.0
17	699.0
18	1326.0
19	2644.0
20	4312.0
21	6470.0
22	8989.0
23	13159.0
24	19229.0
25	27180.0
26	35115.0
27	35170.0
28	30809.0
29	27090.0
30	27075.0
31	29499.0
32	34134.0
33	39405.0
34	46003.0
35	54856.0
36	59969.0
37	61558.0
38	47680.0
39	7271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.10068459594657	2.2553955443246867	29.883708937135783	40.760210922592954
2	39.457093496983134	18.419949976793358	29.520460522923003	12.602496003300502
3	9.520524985818163	18.694400752926615	58.758895879531735	13.02617838172348
4	8.933912639884483	2.352734516012583	74.28139987623125	14.431952967871691
5	21.220540456913	2.7520821515135885	60.07490588417307	15.95247150740034
6	11.63474678974782	14.849994842968387	63.305947346707235	10.209311020576555
7	47.38812464545408	0.8850755505131246	49.20356093032851	2.523238873704296
8	46.443743231396006	11.169163529472435	39.030832602753854	3.356260636377701
9	43.84508277035738	2.311155948635965	37.40685111649734	16.436910164509307
10	26.346791037079058	17.81561033469135	43.571921252127275	12.265677376102316
11	20.010088443092155	15.446115465937806	51.58562606363777	12.958170027332267
12	15.614041307823218	13.818749677685524	53.027177556598424	17.540031457892837
13	14.97424707338456	14.892862668248155	59.484586921767836	10.648303336599453
14	13.509650095405085	21.147052756433396	49.98871899334743	15.354578154814089
15	10.36192692486205	15.403731112371718	60.194968026404005	14.039373936362228
16	11.949003403640864	18.234941467691197	48.97810195451498	20.837953174152958
17	12.575099272858543	17.998684956938785	54.41731989067093	15.008895879531742
18	12.576549687999588	17.059460574493322	53.05699164560879	17.306998091898304
19	12.30628900005157	19.076826878448767	49.90830153163839	18.708582589861276
20	14.615349904594915	18.61285519055232	55.365085606724776	11.406709298127996
21	14.481267082667218	22.341711360940643	51.368708421432615	11.808313134959517
22	12.724330875148265	14.394081016966634	53.73433551647672	19.147252591408385
23	13.217794337579289	21.36928858748904	52.63008612242793	12.782830952503737
24	15.493012222164923	16.970501779175905	50.08847532360373	17.44801067505544
25	11.861172708988706	26.096836161105667	47.882716208550356	14.159274921355266
26	12.712244082306224	16.100897323500593	52.38609406425662	18.80076452993657
27	16.161975916662367	18.054123046774276	48.73024212263421	17.053658913929144
28	10.764981176834613	18.197714145737713	53.04329328038781	17.994011397039863
29	15.077065391160849	15.337334330359445	49.299288329637456	20.286311948842247
30	12.317086534990459	19.966253674385023	53.32145067299262	14.395209117631891
31	14.705436800577587	16.718290701872	46.69498736527255	21.88128513227786
32	16.322488525604662	24.29219741117013	44.451356299314114	14.933957763911094
33	13.283224176164198	18.152751276365326	43.21286292609974	25.35116162137074
34	15.667223196328193	20.690655458717963	44.61573668196586	19.026384662987983
35	13.139633077200763	25.42207080604404	42.7828954153989	18.6554007013563
36	14.710271517714405	20.94254422154608	37.331913000876696	27.01527125986282
37	14.675622711567222	25.641728121293383	43.51148728791707	16.17116187922232
38	16.581629364138	22.593761281006653	35.77561755453561	25.048991800319737
39	18.613983291217576	24.648193749677684	39.09497318343561	17.642849775669127
40	17.193221081945232	25.4547857253365	38.37862926099737	18.9733639317209
41	17.054625857356505	24.576156464339128	37.416681708008866	20.952535970295497
42	16.75148909287814	25.770492754370583	40.08770176886184	17.390316383889434
43	19.498414212779124	21.487416842865247	34.60207054819246	24.412098396163167
44	19.976245423134444	22.194413645505648	36.33547779897891	21.493863132381
45	16.63481125264298	22.00344231860141	36.17883296374607	25.182913465009545
46	22.206016966634007	24.93537594760456	33.651242844618636	19.2073642411428
47	15.57375199834975	21.187019751431077	43.38787968645248	19.851348563766695
48	18.95821515135888	23.82145711928214	34.32568588520448	22.894641844154503
49	16.58034010623485	22.165244185446856	40.32637563818266	20.92804007013563
50	18.373053220566245	20.348679799907174	37.8047483368573	23.47351864266928
51	18.23623072559435	20.96591202104069	35.22752178845857	25.570335464906403
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	27.0
1	787.5
2	1548.0
3	20623.5
4	39699.0
5	30179.5
6	20660.0
7	19884.0
8	19108.0
9	18882.0
10	18656.0
11	18337.5
12	18019.0
13	17212.5
14	16406.0
15	15653.0
16	14900.0
17	14034.5
18	13169.0
19	12184.5
20	11200.0
21	10305.5
22	9411.0
23	8903.5
24	8396.0
25	8016.5
26	7769.5
27	7902.0
28	8233.5
29	8565.0
30	9356.0
31	10147.0
32	10859.0
33	11571.0
34	12712.0
35	13853.0
36	14638.0
37	15423.0
38	16297.5
39	17172.0
40	18347.5
41	19523.0
42	20568.0
43	21613.0
44	23815.5
45	26018.0
46	64454.0
47	102890.0
48	70686.5
49	38483.0
50	34948.0
51	31413.0
52	27421.0
53	23429.0
54	21451.5
55	19474.0
56	17786.0
57	16098.0
58	14232.0
59	12366.0
60	10621.5
61	8877.0
62	7483.0
63	6089.0
64	5069.0
65	4049.0
66	3320.0
67	2591.0
68	2032.5
69	1474.0
70	1201.0
71	928.0
72	766.0
73	604.0
74	541.5
75	374.5
76	270.0
77	225.5
78	181.0
79	141.0
80	101.0
81	79.5
82	58.0
83	39.5
84	21.0
85	14.5
86	8.0
87	5.5
88	3.0
89	3.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	620512.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.76835831791748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.49091321980131	41.37159881997193
2	7.778183285934372	7.89771192245506
3	3.710209883340289	5.650837943762959
4	2.28484300386253	4.6399091328111925
5	1.4404234994224847	3.6563968174114656
6	0.9741305683923474	2.9673005846747587
7	0.6450626543884284	2.292414038284909
8	0.4337977317445996	1.7618558946167748
9	0.3108113501283908	1.420144379333447
>10	0.8488711084146691	6.769539593848034
>50	0.04862182144603398	1.7377234285332481
>100	0.028979892275575153	3.0322700649808154
>500	0.0012879952122477794	0.40230829855613615
>1k	0.0025759904244955587	4.032237501903964
>5k	0.0	0.0
>10k+	0.0012879952122477794	12.36775157885536
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21128	3.404930122221649	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTC	20827	3.356421793615595	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCG	19171	3.089545407663349	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGC	14530	2.341614666597906	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCT	4844	0.780645660357898	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTC	3799	0.6122363467588057	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTTCTGC	3576	0.576298282708473	TruSeq Adapter, Index 23 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACTACT	3177	0.5119965447888196	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCT	3060	0.49314114795523695	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACTACTCGTA	2644	0.4260997369913877	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTATGCCGTCTT	2276	0.3667938734464442	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTCACTACTCGT	1290	0.2078928368830901	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTCACTACTCGTAT	674	0.10861997834046722	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	626	0.10088443092156155	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.2231447578773655E-4	0.0	0.0	0.06752488267753082	0.0
2	3.2231447578773655E-4	0.0	0.0	0.7134430921561549	0.0
3	3.2231447578773655E-4	0.0	0.0	0.8386622659996906	0.0
4	3.2231447578773655E-4	0.0	0.0	1.7745023464493836	0.0
5	3.2231447578773655E-4	0.0	0.0	5.695296787169306	0.0
6	3.2231447578773655E-4	0.0	0.0	6.223731370223299	0.0
7	3.2231447578773655E-4	0.0	0.0	6.909294260223815	0.0
8	3.2231447578773655E-4	0.0	0.0	7.736353205095147	0.0
9	3.2231447578773655E-4	0.0	0.0	7.902022845650044	0.0
10	3.2231447578773655E-4	0.0	0.0	11.582531844670207	0.0
11	3.2231447578773655E-4	0.0	0.0	11.764156051776597	0.0
12	3.2231447578773655E-4	0.0	0.0	14.674978082615647	0.0
13	3.2231447578773655E-4	0.0	0.0	14.89882548605023	0.0
14	3.2231447578773655E-4	0.0	0.0	15.078515806301892	0.0
15	3.2231447578773655E-4	0.0	0.0	15.649818214635657	0.0
16	3.2231447578773655E-4	0.0	0.0	15.96745913052447	0.0
17	3.2231447578773655E-4	0.0	0.0	16.13119488422464	0.0
18	3.2231447578773655E-4	0.0	0.0	16.243199164560878	0.0
19	3.2231447578773655E-4	0.0	0.0	16.995964622763136	0.0
20	3.2231447578773655E-4	0.0	0.0	17.150353256665465	0.0
21	3.2231447578773655E-4	0.0	0.0	17.252849259965963	0.0
22	3.2231447578773655E-4	0.0	0.0	17.476696663400546	0.0
23	3.2231447578773655E-4	0.0	0.0	17.578709194987365	0.0
24	3.2231447578773655E-4	0.0	0.0	17.67846552524367	0.0
25	3.2231447578773655E-4	0.0	0.0	17.76001108761797	0.0
26	3.2231447578773655E-4	0.0	0.0	17.874916198236296	0.0
27	3.2231447578773655E-4	0.0	0.0	18.050255273064824	0.0
28	3.2231447578773655E-4	0.0	0.0	18.138247124954876	0.0
29	3.2231447578773655E-4	0.0	0.0	18.23961502759012	0.0
30	4.834717136816049E-4	0.0	0.0	18.351619307926356	0.0
31	4.834717136816049E-4	0.0	0.0	18.459433500077356	1.6115723789386828E-4
32	4.834717136816049E-4	0.0	0.0	18.575305554123048	1.6115723789386828E-4
33	4.834717136816049E-4	0.0	0.0	18.68876024960033	1.6115723789386828E-4
34	4.834717136816049E-4	0.0	0.0	18.859909236243617	1.6115723789386828E-4
35	4.834717136816049E-4	0.0	0.0	18.986739982466094	1.6115723789386828E-4
36	4.834717136816049E-4	0.0	0.0	19.104707080604403	1.6115723789386828E-4
37	4.834717136816049E-4	0.0	0.0	19.239756845959466	1.6115723789386828E-4
38	4.834717136816049E-4	0.0	0.0	19.38818266205972	1.6115723789386828E-4
39	4.834717136816049E-4	0.0	0.0	19.573513485637665	1.6115723789386828E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACCGGT	130	0.0	45.000004	16
CGACGAA	25	3.8887243E-5	45.0	19
ATTCGGC	25	3.8887243E-5	45.0	28
TACGCCA	20	7.030774E-4	45.0	28
GAGCGAC	25	3.8887243E-5	45.0	18
GCGATAG	20	7.030774E-4	45.0	9
GACGAAT	25	3.8887243E-5	45.0	20
ACCGGTG	105	0.0	44.999996	17
GCGATCG	30	2.1638807E-6	44.999996	9
CGTTTTT	18345	0.0	44.582993	1
CGATGAA	560	0.0	42.589283	19
GACACGA	90	0.0	42.5	25
TGATACC	2235	0.0	42.18121	4
GATACCT	2235	0.0	42.18121	5
ACGGCTG	1885	0.0	42.015915	8
ATACCTG	2265	0.0	41.821194	6
CCGATGA	565	0.0	41.81416	18
CGGCTGT	1940	0.0	41.52062	9
TACGGCT	1935	0.0	40.930237	7
CGCTTTT	530	0.0	40.75472	1
>>END_MODULE