##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933243.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	295143
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.537929071670344	31.0	28.0	31.0	16.0	33.0
2	28.7102658711201	31.0	30.0	33.0	16.0	34.0
3	28.88011235231735	31.0	30.0	33.0	16.0	34.0
4	32.01978363030802	35.0	30.0	37.0	19.0	37.0
5	33.6532697709246	35.0	33.0	37.0	28.0	37.0
6	32.58633611503576	35.0	33.0	37.0	17.0	37.0
7	32.16417465432011	35.0	32.0	35.0	25.0	37.0
8	32.806720809912484	35.0	32.0	36.0	27.0	37.0
9	33.06247141216292	35.0	31.0	37.0	25.0	39.0
10	31.524715138085607	35.0	27.0	39.0	15.0	39.0
11	32.408845881487956	35.0	28.0	39.0	17.0	39.0
12	33.21704732959955	35.0	31.0	39.0	23.0	39.0
13	33.45657189904555	35.0	32.0	39.0	25.0	39.0
14	33.6570374360903	37.0	32.0	40.0	20.0	41.0
15	34.214482471208875	37.0	32.0	40.0	25.0	41.0
16	34.409245687683594	37.0	32.0	40.0	25.0	41.0
17	32.32561165265651	35.0	27.0	40.0	18.0	41.0
18	33.03273328522106	36.0	30.0	39.0	23.0	39.0
19	32.76735006420616	36.0	30.0	37.0	21.0	39.0
20	32.270357081143715	34.0	30.0	36.0	23.0	39.0
21	32.716530630914505	35.0	31.0	37.0	24.0	39.0
22	32.83236939381927	34.0	30.0	37.0	25.0	39.0
23	33.823783725177286	35.0	32.0	38.0	27.0	40.0
24	32.1496494919412	35.0	31.0	37.0	21.0	39.0
25	31.355078724550474	34.0	29.0	37.0	18.0	39.0
26	30.454522045245863	33.0	25.0	37.0	18.0	39.0
27	32.20340309612629	34.0	30.0	37.0	22.0	39.0
28	32.262218653330756	35.0	31.0	37.0	21.0	39.0
29	32.40203562340967	35.0	31.0	38.0	21.0	40.0
30	31.402547239812566	34.0	29.0	37.0	18.0	40.0
31	31.746184053153893	35.0	30.0	37.0	20.0	39.0
32	31.554571851610913	35.0	30.0	37.0	18.0	40.0
33	31.33580332245725	35.0	30.0	38.0	15.0	40.0
34	30.40894075075472	35.0	27.0	38.0	10.0	40.0
35	29.840856805006386	35.0	25.0	38.0	8.0	40.0
36	29.216583147830036	34.0	23.0	38.0	7.0	40.0
37	29.326350277662016	35.0	23.0	38.0	7.0	40.0
38	29.170585106202758	34.0	23.0	38.0	7.0	40.0
39	28.74551996828656	34.0	22.0	37.0	7.0	40.0
40	28.490701117763255	33.0	21.0	37.0	7.0	40.0
41	27.985247829018476	33.0	20.0	37.0	7.0	40.0
42	28.42134490738388	33.0	21.0	37.0	7.0	40.0
43	28.29101147579309	34.0	20.0	37.0	7.0	40.0
44	28.275818840358742	34.0	19.0	38.0	7.0	40.0
45	28.502176910853382	34.0	20.0	38.0	7.0	40.0
46	28.184676580505045	34.0	20.0	38.0	7.0	40.0
47	28.18483582534568	34.0	20.0	38.0	7.0	40.0
48	28.00605130394419	34.0	20.0	37.0	7.0	40.0
49	27.86479435392335	33.0	19.0	37.0	7.0	39.0
50	27.96617910639927	34.0	20.0	37.0	7.0	40.0
51	26.406538525392776	31.0	18.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	10.0
10	14.0
11	22.0
12	35.0
13	46.0
14	75.0
15	144.0
16	343.0
17	645.0
18	1109.0
19	1782.0
20	2728.0
21	3678.0
22	5173.0
23	7086.0
24	9681.0
25	13149.0
26	16785.0
27	17853.0
28	17559.0
29	17453.0
30	18974.0
31	21132.0
32	23468.0
33	25849.0
34	28016.0
35	29310.0
36	20437.0
37	10444.0
38	2095.0
39	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.148924419688086	2.475071406064179	32.72752530129463	42.64847887295311
2	42.771470100934124	12.460400551597022	32.34127185804847	12.426857489420383
3	11.856625432417506	12.361804277926293	62.94406440267938	12.837505886976821
4	10.739878635102308	2.662099389109686	71.42876503931991	15.169256936468084
5	18.743117742924618	3.1340739912516984	62.518169158679015	15.604639107144672
6	13.077389604361276	11.35619005024683	64.4057287484372	11.160691596954697
7	49.48618127483965	1.1543556852102201	46.7471700158906	2.612293024059524
8	50.49924951633615	8.66698515634794	37.35477378762159	3.478991539694318
9	47.28385901071684	2.3510637216535715	37.31852017496603	13.046557092663559
10	28.840934733332656	15.478259691065011	42.9025929803519	12.778212595250437
11	21.894471493479433	15.45352591794486	49.453654669092614	13.198347919483098
12	16.879275469856985	13.80991587128951	52.22180434569006	17.08900431316345
13	15.68832735318134	14.639005499029285	57.78656447891362	11.886102668875766
14	13.942732844756609	17.84999136012035	51.44252108300045	16.7647547121226
15	11.129520266447113	14.482132390061766	59.50539230135901	14.882955042132117
16	12.638280426776172	13.985424014799605	51.8548635746062	21.52143198381801
17	13.065530946015999	15.351202637365615	54.653168125281645	16.930098291336744
18	13.428744710191332	14.463836174329053	53.82712786683066	18.28029124864896
19	14.368966907566842	16.97888819995731	50.56633564068943	18.085809251786422
20	16.78169565261585	15.42337104386687	55.13022500957163	12.664708293945647
21	16.60517105267616	19.180194007650528	50.26783626919832	13.94679867047499
22	14.064368797498162	14.585133308260742	52.99600532623169	18.354492568009405
23	14.314078260368705	19.837163679978858	50.779791490904415	15.06896656874803
24	15.112335376410757	15.470128039628248	50.76183409398156	18.655702489979433
25	12.710110014467563	20.80787957024222	49.474322616494376	17.007687798795835
26	13.601203484412641	16.552654137147076	52.32073943817065	17.525402940269633
27	14.893458425237935	17.794763894112346	49.77485490084467	17.536922779805046
28	11.934214939876602	17.645005980151996	54.099877008772026	16.320902071199384
29	15.956333031784592	16.069159695469654	50.73506740800222	17.23943986474353
30	15.059818460881674	19.54001958372721	50.49552250942764	14.904639445963483
31	15.851976838346157	16.412721968672813	47.80326824623996	19.93203294674107
32	16.817610446461543	20.79432681784762	47.466821168043964	14.92124156764687
33	15.070660662797355	20.059767638060194	44.229068620973564	20.640503078168887
34	16.359866234333865	22.596504067519813	42.55428724381063	18.489342454335695
35	16.878597832237254	21.59258393388967	41.454481386988675	20.074336846884393
36	16.397475122228887	24.400036592431466	38.84184954411929	20.360638741220356
37	17.78561578624599	21.740308934990836	43.61038547415998	16.863689804603194
38	16.86673917389198	23.98972701368489	38.827957972914824	20.315575839508305
39	16.968723635661355	22.413541910192684	41.15394910263838	19.463785351507575
40	17.350572434379266	23.16605848690296	39.68788011235232	19.795488966365458
41	16.331744273115067	24.52777128375059	37.99412488183694	21.146359561297405
42	16.272450981388683	22.0828547517644	41.06721148731293	20.57748277953399
43	17.93808425068526	24.385806202417136	37.98802614325937	19.688083403638238
44	19.65013569693335	21.827385369126155	38.7205524101876	19.80192652375289
45	16.465238884201895	21.62849872773537	38.184541052981096	23.72172133508164
46	19.32656373351223	23.992098745353946	37.44761014152462	19.233727379609206
47	15.719498683688924	22.22312573904853	42.99746224711411	19.059913330148436
48	18.46630277526487	22.32172201271926	36.81130841659805	22.400666795417816
49	16.21248005204257	20.357589371931574	41.22882805961856	22.2011025164073
50	17.696167620441617	21.101974297205082	39.2857021850425	21.916155897310794
51	17.71920729951244	20.595440176456837	37.77694202471344	23.90841049931728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	95.0
1	322.0
2	549.0
3	15113.5
4	29678.0
5	20148.5
6	10619.0
7	10193.0
8	9767.0
9	9626.0
10	9485.0
11	8854.0
12	8223.0
13	7550.5
14	6878.0
15	6271.5
16	5665.0
17	5251.0
18	4837.0
19	4398.5
20	3960.0
21	3641.0
22	3322.0
23	3103.5
24	2885.0
25	2841.0
26	2868.0
27	2939.0
28	2922.0
29	2905.0
30	3318.5
31	3732.0
32	3961.5
33	4191.0
34	4921.5
35	5652.0
36	5649.5
37	5647.0
38	6205.0
39	6763.0
40	7323.0
41	7883.0
42	8511.0
43	9139.0
44	10516.0
45	11893.0
46	21616.0
47	31339.0
48	26043.5
49	20748.0
50	19138.0
51	17528.0
52	15113.5
53	12699.0
54	11492.5
55	10286.0
56	9816.0
57	9346.0
58	8853.0
59	8360.0
60	7532.0
61	6704.0
62	5970.5
63	5237.0
64	4627.0
65	4017.0
66	3443.5
67	2870.0
68	2455.5
69	2041.0
70	1727.5
71	1414.0
72	1278.5
73	1143.0
74	982.0
75	663.0
76	505.0
77	373.5
78	242.0
79	224.5
80	207.0
81	146.5
82	86.0
83	59.5
84	33.0
85	21.0
86	9.0
87	6.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	295143.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.11630797498363
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.72072787539696	38.50378982517032
2	6.638653195456098	6.255776569924374
3	2.9327237805766537	4.145373505536238
4	1.900837566697696	3.5824179281178856
5	1.3816557318218339	3.254925848795946
6	1.046217974338608	2.9576356972740827
7	0.8580815064154179	2.8300742766734706
8	0.7228646619033489	2.724697122757648
9	0.5464566629365698	2.317231838331112
>10	2.163974211652119	15.228791712370981
>50	0.046443283466964566	1.4712468298589312
>100	0.03338110999188078	2.9315524794818675
>500	0.0029027052166852854	0.909485607117071
>1k	0.0029027052166852854	2.3492765437975174
>5k	0.0014513526083426427	3.6776042068237653
>10k+	7.256763041713213E-4	6.8601200079687645
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20064	6.798060601132333	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCG	5740	1.9448199686253782	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGC	5016	1.6995151502830832	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	3221	1.091335386575321	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTACCAT	1329	0.45029019831064937	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGC	1261	0.42725051923982615	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCT	1060	0.35914793845695137	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTC	841	0.28494661909650576	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTA	782	0.2649563093144679	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCAGCTACCATCGTATGCCG	524	0.17754105636928538	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCT	513	0.17381404946076986	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTC	389	0.13180051703750387	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	388	0.1314616982276388	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTACCATCGT	355	0.1202806775020922	No Hit
CTGTCTCTTATACACATCTGACGCAGCTACCATCGTATGCCGTCTTCTGCT	334	0.11316548249492618	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	321	0.10876083796668055	No Hit
GAATGATACGGCTGCCTCCTATACACATCTGACGCAGCTACCATCGTATGC	305	0.10333973700883978	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13756043680520968	0.0
2	0.0	0.0	0.0	0.7948689279434037	0.0
3	0.0	0.0	0.0	0.9642783328759279	0.0
4	0.0	0.0	0.0	1.5836391173092366	0.0
5	0.0	0.0	0.0	3.0554680273630073	0.0
6	0.0	0.0	0.0	3.3166973297689593	0.0
7	0.0	0.0	0.0	3.66974652964834	0.0
8	0.0	0.0	0.0	4.1983038730378155	0.0
9	0.0	0.0	0.0	4.339252497941676	0.0
10	0.0	0.0	0.0	6.986782678227164	0.0
11	0.0	0.0	0.0	7.502464906841769	0.0
12	0.0	0.0	0.0	9.900963261876447	0.0
13	0.0	0.0	0.0	10.164564295951454	0.0
14	0.0	0.0	0.0	10.317710398010457	0.0
15	0.0	0.0	0.0	10.773082878469081	0.0
16	0.0	0.0	0.0	11.067177605431944	0.0
17	0.0	0.0	0.0	11.334166827605602	0.0
18	0.0	0.0	0.0	11.598106680490474	0.0
19	0.0	0.0	0.0	12.356721995778317	0.0
20	0.0	0.0	0.0	12.632859325818332	0.0
21	0.0	0.0	0.0	12.924243502302273	0.0
22	0.0	0.0	0.0	13.364369136316972	0.0
23	0.0	0.0	0.0	13.642200560406312	0.0
24	0.0	0.0	0.0	13.857689323480482	0.0
25	0.0	0.0	0.0	14.05081604510356	0.0
26	0.0	0.0	0.0	14.241232216247717	0.0
27	0.0	0.0	0.0	14.424533192384708	0.0
28	0.0	0.0	0.0	14.601057792324399	0.0
29	0.0	0.0	0.0	14.78639168132058	0.0
30	0.0	0.0	0.0	14.99442643057772	0.0
31	0.0	0.0	0.0	15.187891971010663	0.0
32	0.0	0.0	0.0	15.390844438119826	0.0
33	0.0	0.0	0.0	15.579905334024524	0.0
34	0.0	0.0	0.0	15.776081424936386	0.0
35	0.0	0.0	0.0	15.988520818721772	0.0
36	0.0	0.0	0.0	16.19045682940134	0.0
37	0.0	0.0	0.0	16.392731658890774	0.0
38	0.0	0.0	0.0	16.583486648844797	0.0
39	0.0	0.0	0.0	16.821337453370063	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTCG	30	2.159999E-6	45.000004	1
ACCGCCT	30	2.159999E-6	45.000004	14
GACCGCC	35	1.2078272E-7	45.0	13
GCGTTGG	20	7.024758E-4	45.0	2
GCCGATT	20	7.024758E-4	45.0	9
CGGGATC	55	1.8189894E-12	45.0	6
CGTGTCT	20	7.024758E-4	45.0	20
TTCGGGT	35	1.2078272E-7	45.0	4
GTCTTAT	70	0.0	45.0	23
CGCCACT	20	7.024758E-4	45.0	20
CGTTTTT	9675	0.0	44.255814	1
TACGGCT	785	0.0	43.28026	7
ACGGCTG	795	0.0	42.73585	8
CCGATGA	95	0.0	42.63158	18
CGATGAA	90	0.0	42.5	19
ACCGGTG	70	0.0	41.785713	17
CCTATAC	70	0.0	41.785713	18
CGGCTGT	755	0.0	41.721855	9
ATGATAC	2320	0.0	41.702587	3
GATACGG	1610	0.0	41.645966	5
>>END_MODULE