##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933242.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	604801
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.524704820263192	31.0	28.0	33.0	25.0	34.0
2	28.820445072015424	31.0	28.0	33.0	16.0	34.0
3	28.792316811645485	31.0	28.0	34.0	16.0	34.0
4	33.18577350235863	35.0	32.0	37.0	28.0	37.0
5	34.24306176742433	35.0	33.0	37.0	30.0	37.0
6	33.72919852976433	35.0	33.0	37.0	28.0	37.0
7	33.59370602892522	35.0	32.0	37.0	29.0	37.0
8	33.55144254060426	35.0	33.0	37.0	28.0	37.0
9	34.71756660455257	37.0	34.0	39.0	28.0	39.0
10	34.384743080781945	37.0	32.0	39.0	27.0	39.0
11	34.59000067790893	37.0	33.0	39.0	27.0	39.0
12	34.708914171768896	37.0	33.0	39.0	27.0	39.0
13	34.790645187425284	37.0	34.0	39.0	27.0	39.0
14	35.677150004712296	38.0	34.0	40.0	27.0	41.0
15	35.940560614152425	38.0	34.0	40.0	27.0	41.0
16	35.907949887648996	38.0	34.0	40.0	27.0	41.0
17	35.262836867002534	37.0	33.0	40.0	27.0	41.0
18	35.05953693859633	37.0	33.0	39.0	27.0	40.0
19	34.355971633644785	37.0	33.0	38.0	27.0	40.0
20	33.70325445890467	35.0	32.0	38.0	27.0	40.0
21	34.135067567679286	35.0	33.0	38.0	27.0	40.0
22	34.69403985773833	35.0	33.0	39.0	29.0	40.0
23	35.06644830283019	35.0	33.0	39.0	30.0	40.0
24	34.56313564296355	35.0	33.0	39.0	27.0	40.0
25	33.58479566006009	35.0	33.0	38.0	24.0	40.0
26	33.56417896134431	35.0	32.0	38.0	24.0	40.0
27	34.25071883148341	35.0	33.0	38.0	27.0	40.0
28	34.06716424079987	35.0	33.0	39.0	25.0	40.0
29	34.1399997685189	35.0	33.0	39.0	25.0	40.0
30	33.379751356231225	35.0	33.0	39.0	22.0	40.0
31	33.25746981238457	35.0	33.0	38.0	22.0	40.0
32	32.66607363413751	35.0	32.0	38.0	18.0	40.0
33	32.24425058821001	35.0	31.0	39.0	15.0	40.0
34	31.65988812849185	35.0	30.0	39.0	10.0	40.0
35	31.20734258045208	35.0	29.0	39.0	8.0	40.0
36	30.605195758604896	35.0	26.0	39.0	7.0	40.0
37	30.512102327873134	35.0	25.0	39.0	7.0	40.0
38	30.232370647535305	35.0	24.0	39.0	7.0	40.0
39	29.899902612594886	35.0	23.0	39.0	7.0	40.0
40	29.631022435478776	35.0	23.0	39.0	7.0	40.0
41	29.163589345917085	35.0	21.0	38.0	7.0	40.0
42	29.32968530144626	35.0	22.0	39.0	7.0	40.0
43	29.242767455741642	35.0	21.0	39.0	7.0	40.0
44	29.332725971021873	35.0	21.0	39.0	7.0	40.0
45	29.451343499762732	35.0	22.0	39.0	7.0	40.0
46	29.295723717388032	35.0	22.0	39.0	7.0	40.0
47	29.26036497955526	35.0	22.0	38.0	7.0	40.0
48	29.1186125684316	35.0	22.0	38.0	7.0	40.0
49	29.328243504888384	35.0	22.0	38.0	7.0	40.0
50	29.276927452170217	35.0	22.0	38.0	7.0	40.0
51	28.088730012020484	33.0	20.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	13.0
9	29.0
10	37.0
11	34.0
12	35.0
13	65.0
14	99.0
15	165.0
16	309.0
17	590.0
18	1234.0
19	2183.0
20	3472.0
21	5212.0
22	7429.0
23	10728.0
24	15572.0
25	22395.0
26	29066.0
27	31230.0
28	29967.0
29	28112.0
30	29090.0
31	32543.0
32	37908.0
33	43816.0
34	48855.0
35	56626.0
36	59488.0
37	59180.0
38	43236.0
39	6081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.17720539483235	2.377310884075919	32.95480662234355	38.49067709874818
2	37.44471321972021	16.135224644139146	32.23655384167685	14.183508294463799
3	12.297764057929799	16.249642444374267	57.20377446465862	14.248819033037313
4	11.365721948211064	2.7809147140960415	69.35636680494906	16.49699653274383
5	20.944079126853296	3.270993268860336	58.39061112663504	17.394316477651326
6	13.802721887033917	13.77097590777793	61.40763656144749	11.018665643740668
7	51.94998024143479	1.3578019877612635	43.76811546277205	2.924102308031898
8	51.05861266763778	10.240723808327036	35.286482661238985	3.4141808627961923
9	48.11417309164502	2.668481037564422	34.31244326646286	14.904902604327704
10	32.38552846308124	14.032549549355904	40.81821954659466	12.763702440968187
11	26.40885183721588	14.635061780651817	45.21619507904253	13.739891303089777
12	19.894643031344195	13.360758332079477	49.32746473633476	17.417133900241566
13	18.727647606402765	13.740883364941524	55.22328832128254	12.308180707373168
14	15.812308511394658	19.140345336730594	49.10524288154286	15.942103270331895
15	13.208476837835917	13.852655666905312	57.67897209164667	15.259895403612097
16	16.20169278820637	16.262208561163092	46.8192016878279	20.716896962802643
17	16.667465827602797	16.367367117448552	50.924022943083756	16.041144111864895
18	16.743358559261644	15.633076003511897	49.671710198891866	17.951855238334595
19	16.380759952447168	17.924242850127563	46.63798505624164	19.05701214118363
20	17.61124733590057	18.821893482318977	51.014300571592976	12.552558610187484
21	17.439124604622016	21.383562527178363	48.287287884775324	12.8900249834243
22	15.719881415539987	14.032714892997863	51.237018457310754	19.010385234151396
23	15.872824284351381	19.897288529615526	50.25123966395558	13.978647522077509
24	17.90076405296949	16.737075500867228	47.021086274658934	18.341074171504346
25	15.603644835243328	23.60263954590022	44.80134788136924	15.992367737487207
26	15.324875454901695	14.691278618917627	49.614170611490394	20.369675314690287
27	18.116537505725024	16.76121567259313	46.412125641326654	18.71012118035519
28	13.048754879704234	18.15588929251109	50.20279397686181	18.592561850922866
29	18.717561644243315	16.04808854482714	48.38318719711111	16.85116261381843
30	19.63422679525993	19.36471665886796	47.270093799448084	13.73096274642403
31	19.59900859952282	18.27014174910425	44.16692432717538	17.963925324197547
32	24.691758115479306	19.427877930096017	42.12112744522579	13.759236509198894
33	22.183164379688524	16.945739177018556	39.78134956787439	21.089746875418527
34	19.7226856437076	19.823710608944097	42.72479708201541	17.728806665332893
35	20.291633115685986	24.081144045727438	38.27787983154789	17.34934300703868
36	19.087766058587867	24.131904543808623	40.22331312282883	16.55701627477468
37	23.46358554301332	22.614049910631763	37.58409790989102	16.338266636463892
38	23.39794411715589	24.771123063619275	34.0242492985296	17.806683520695238
39	23.638353772563207	20.99136740845336	34.76052453616975	20.609754282813686
40	23.477805096221733	23.33147597308867	36.23985410077034	16.95086482991926
41	19.112567604881605	23.48673365288748	36.16131587084016	21.239382871390756
42	18.893817966570822	24.277241605089937	38.87642381543681	17.952516612902425
43	21.222517819911012	20.27708287519366	37.84699430060466	20.65340500429067
44	22.596854171868102	20.482935709431697	35.13816941440243	21.782040704297778
45	18.671761455420874	21.28848993305236	35.31128420753273	24.728464403994042
46	23.653730731265327	22.387363777506984	34.23969206400122	19.719213427226475
47	17.60463359022224	21.005256274377853	42.51018103475358	18.87992910064633
48	19.38257377219945	23.797745043410973	34.237542596655764	22.582138587733816
49	18.875299478671497	21.04262393746042	39.93644190403124	20.14563467983684
50	19.411839596826063	20.010879611640853	38.0508630111392	22.52641778039388
51	18.73508807029089	20.714747495457182	35.78482839810119	24.765336036150735
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	1080.5
2	2076.0
3	23684.5
4	45293.0
5	32282.0
6	19271.0
7	17825.5
8	16380.0
9	15881.5
10	15383.0
11	14655.5
12	13928.0
13	13223.0
14	12518.0
15	11603.5
16	10689.0
17	9969.0
18	9249.0
19	8519.5
20	7790.0
21	7273.0
22	6756.0
23	6435.5
24	6115.0
25	6153.0
26	6444.0
27	6697.0
28	7149.5
29	7602.0
30	8022.0
31	8442.0
32	8934.0
33	9426.0
34	10253.5
35	11081.0
36	11599.5
37	12118.0
38	12578.5
39	13039.0
40	13831.0
41	14623.0
42	15676.0
43	16729.0
44	18795.5
45	20862.0
46	52777.0
47	84692.0
48	59477.5
49	34263.0
50	31966.0
51	29669.0
52	27164.0
53	24659.0
54	24691.0
55	24723.0
56	24757.0
57	24791.0
58	23285.0
59	21779.0
60	19579.0
61	17379.0
62	15628.0
63	13877.0
64	12339.0
65	10801.0
66	9410.5
67	8020.0
68	6915.0
69	5810.0
70	5092.5
71	4375.0
72	3655.0
73	2935.0
74	2513.0
75	1637.0
76	1183.0
77	948.0
78	713.0
79	539.5
80	366.0
81	274.5
82	183.0
83	135.5
84	88.0
85	60.5
86	33.0
87	26.0
88	19.0
89	13.0
90	7.0
91	4.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	604801.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.2765401194923
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.74177162132618	37.699150652550195
2	7.321623619433064	6.9228206556789935
3	3.835684604104675	5.4401369121502094
4	2.5281067337343845	4.780805576950091
5	1.8301222240314219	4.3260923373998
6	1.3072092583700987	3.708019856874346
7	0.9116792233943084	3.0170727566636026
8	0.6527510486584307	2.468784891195278
9	0.4452266772242878	1.8943899181256065
>10	1.3221227762898777	9.201161142475248
>50	0.06001943191203032	2.010603262643962
>100	0.03764532391265094	3.1652519849508627
>500	0.0017757228260684395	0.5149494927776519
>1k	0.0028411565217095034	3.3929244701828463
>5k	0.0	0.0
>10k+	0.0014205782608547517	11.457836089381324
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23444	3.8763163420695403	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTC	16686	2.7589240097155923	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCG	15172	2.5085937357907806	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGC	12940	2.1395467269399355	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCT	4033	0.6668309080176785	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTC	2930	0.4844568709377134	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGCATT	2894	0.4785044998272159	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTTCTGC	2832	0.46825319402580357	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCT	2548	0.4212955997096566	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGCATTCGTA	2209	0.3652441050858051	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTATGCCGTCTT	1606	0.26554188898497194	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGGCATTCGT	1156	0.19113725010375313	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	764	0.12632254245611366	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAGGCATTCGTAT	653	0.10796939819874636	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0646493640056812	0.0
2	0.0	0.0	0.0	0.6094566642581609	0.0
3	0.0	0.0	0.0	0.7104816294946602	0.0
4	0.0	0.0	0.0	1.5074379837334926	0.0
5	0.0	0.0	0.0	4.707333486551775	0.0
6	0.0	0.0	0.0	5.097048450647403	0.0
7	0.0	0.0	0.0	5.672940355588036	0.0
8	0.0	0.0	0.0	6.333488205211301	0.0
9	0.0	0.0	0.0	6.477667860998907	0.0
10	0.0	0.0	0.0	9.487418175565185	0.0
11	0.0	0.0	0.0	9.676405958323482	0.0
12	0.0	0.0	0.0	12.30338574175638	0.0
13	0.0	0.0	0.0	12.509899950562252	0.0
14	0.0	0.0	0.0	12.675408936162473	0.0
15	0.0	0.0	0.0	13.171605205679223	0.0
16	0.0	0.0	0.0	13.456988331699186	0.0
17	0.0	0.0	0.0	13.584964310574883	0.0
18	0.0	0.0	0.0	13.686650650379216	0.0
19	0.0	0.0	0.0	14.366543706111598	0.0
20	0.0	0.0	0.0	14.509235269121579	0.0
21	0.0	0.0	0.0	14.613236419913326	0.0
22	0.0	0.0	0.0	14.81958528507724	0.0
23	0.0	0.0	0.0	14.921767655807447	0.0
24	0.0	0.0	0.0	15.016178875365616	0.0
25	0.0	0.0	0.0	15.099016039986706	0.0
26	0.0	0.0	0.0	15.214095214789658	0.0
27	0.0	0.0	0.0	15.355464028663974	0.0
28	0.0	0.0	0.0	15.447560437234726	0.0
29	0.0	0.0	0.0	15.55867136463068	0.0
30	0.0	0.0	0.0	15.691772996407083	0.0
31	0.0	0.0	0.0	15.793790023495331	0.0
32	0.0	0.0	0.0	15.913168132989199	0.0
33	0.0	0.0	0.0	16.03105814970544	0.0
34	0.0	0.0	0.0	16.208967908452532	0.0
35	0.0	0.0	0.0	16.362241464547843	0.0
36	0.0	0.0	0.0	16.49782325095362	0.0
37	0.0	0.0	0.0	16.648947339703472	0.0
38	0.0	0.0	0.0	16.843060775362474	0.0
39	0.0	0.0	0.0	17.2216977154469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAC	25	3.8886054E-5	45.000004	10
GCAACGA	25	3.8886054E-5	45.000004	9
CGTCCAA	25	3.8886054E-5	45.000004	26
CGTTTTT	16090	0.0	44.356747	1
CCGATGA	495	0.0	42.727272	18
CGATGAA	505	0.0	42.32673	19
GATACCT	1700	0.0	42.088234	5
TGATACC	1690	0.0	42.071007	4
TGCCAGT	180	0.0	41.25	24
TAGCATA	180	0.0	41.25	30
ATACCTG	1745	0.0	41.1318	6
ACGGCTG	1530	0.0	40.882355	8
TACGGCT	1525	0.0	40.868855	7
TACCTGT	1800	0.0	40.500004	7
GAATCTG	1975	0.0	40.44304	1
GTTGATC	190	0.0	40.263157	16
CCGTTTG	45	1.925764E-8	40.0	1
AATCTGT	1995	0.0	39.924812	2
GATGAAT	550	0.0	39.68182	20
ACAACGA	40	3.4546065E-7	39.375	13
>>END_MODULE