##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933240.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	425902
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.34854731839719	31.0	28.0	31.0	25.0	34.0
2	28.458095993914093	31.0	26.0	33.0	16.0	34.0
3	28.454219515287555	31.0	26.0	33.0	16.0	34.0
4	33.04914745645712	35.0	32.0	37.0	28.0	37.0
5	34.225072904095306	35.0	33.0	37.0	30.0	37.0
6	33.562002526402786	35.0	33.0	37.0	28.0	37.0
7	33.65590440993468	35.0	33.0	37.0	29.0	37.0
8	33.42399660015684	35.0	33.0	37.0	28.0	37.0
9	34.79071946128452	37.0	34.0	39.0	28.0	39.0
10	34.56720794924654	37.0	32.0	39.0	27.0	39.0
11	34.927358875985554	37.0	34.0	39.0	28.0	39.0
12	34.949394461636714	37.0	34.0	39.0	27.0	39.0
13	35.139100074665066	37.0	34.0	39.0	27.0	39.0
14	35.91478321303962	38.0	34.0	40.0	27.0	41.0
15	36.22099919699837	38.0	34.0	40.0	27.0	41.0
16	36.1769961164775	38.0	34.0	40.0	30.0	41.0
17	35.39683072631732	37.0	33.0	40.0	27.0	41.0
18	35.1684448535109	37.0	33.0	39.0	27.0	40.0
19	34.47920413616278	37.0	33.0	38.0	27.0	40.0
20	33.65759493968096	35.0	32.0	37.0	26.0	40.0
21	34.17802217411517	35.0	33.0	38.0	27.0	40.0
22	34.678693690097724	35.0	33.0	39.0	29.0	40.0
23	34.946856319059314	35.0	33.0	39.0	30.0	40.0
24	34.473639475747945	35.0	33.0	38.0	27.0	40.0
25	33.526205559025314	35.0	33.0	38.0	24.0	40.0
26	33.494733530248745	35.0	32.0	38.0	24.0	40.0
27	34.18997797615414	35.0	33.0	38.0	26.0	40.0
28	33.881186282290294	35.0	33.0	39.0	24.0	40.0
29	33.90501335988091	35.0	33.0	39.0	24.0	40.0
30	33.18112147865002	35.0	32.0	38.0	21.0	40.0
31	33.08620997318632	35.0	32.0	38.0	21.0	40.0
32	32.51524763912825	35.0	31.0	38.0	18.0	40.0
33	31.89327356997619	35.0	31.0	39.0	14.0	40.0
34	31.309735572972187	35.0	30.0	39.0	10.0	40.0
35	30.831890904480375	35.0	27.0	39.0	8.0	40.0
36	30.185735216082573	35.0	23.0	39.0	7.0	40.0
37	30.127313325600724	35.0	23.0	39.0	7.0	40.0
38	29.689940408826445	35.0	22.0	39.0	7.0	40.0
39	29.488919986287925	35.0	21.0	39.0	7.0	40.0
40	29.13297660025076	35.0	21.0	39.0	7.0	40.0
41	28.690013195523854	34.0	20.0	38.0	7.0	40.0
42	28.887070264990538	35.0	20.0	39.0	7.0	40.0
43	28.814882296866415	35.0	19.0	38.0	7.0	40.0
44	28.93328512192946	35.0	18.0	39.0	7.0	40.0
45	28.89251987546431	35.0	20.0	39.0	7.0	40.0
46	28.752755328690636	35.0	20.0	39.0	7.0	40.0
47	28.570659447478526	34.0	20.0	38.0	7.0	40.0
48	28.433348516794943	34.0	18.0	38.0	7.0	40.0
49	28.605028856403585	35.0	19.0	38.0	7.0	40.0
50	28.572892355518405	34.0	20.0	38.0	7.0	40.0
51	27.424562458030252	33.0	18.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	10.0
10	23.0
11	27.0
12	41.0
13	54.0
14	71.0
15	100.0
16	219.0
17	467.0
18	997.0
19	1676.0
20	2786.0
21	4008.0
22	5657.0
23	8253.0
24	11780.0
25	16895.0
26	22272.0
27	23514.0
28	21847.0
29	20554.0
30	20692.0
31	22260.0
32	25523.0
33	29649.0
34	33438.0
35	37385.0
36	40581.0
37	40367.0
38	30471.0
39	4280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.811797080079455	2.351479917915389	29.569478424614115	40.26724457739104
2	38.63682255542355	18.54745927466882	30.806382688975397	12.009335480932233
3	11.072969838131778	18.845415142450612	57.57404285492906	12.507572164488545
4	10.151631126409361	2.591441223567863	72.95504599649685	14.301881653525928
5	22.310531530727726	3.1152706491164635	58.64494649003762	15.929251330118197
6	12.276767894961752	16.211241083629567	61.655263417405884	9.8567276040028
7	48.41536315866091	1.2815154659992205	47.630206009833245	2.672915365506619
8	47.375452568900826	12.523538278758965	36.59996900695465	3.501040145385558
9	45.123760865175555	2.4951749463491604	35.366821475362876	17.014242713112406
10	30.972148522430043	14.16851764020831	41.898605782550916	12.96072805481073
11	24.26990246582547	14.376781513118042	47.59663021070575	13.756685810350739
12	17.400247005179594	12.880427891862448	50.73702401021831	18.982301092739647
13	16.44157576156017	14.323013275354423	57.95276847725533	11.282642485830072
14	14.362928561030472	20.981587313513437	48.73562462726167	15.91985949819442
15	10.74895163676151	14.29342900479453	60.10185441721335	14.855764941230612
16	12.57143662157022	15.790252217646314	48.90092086912012	22.73739029166334
17	13.29648604608572	16.515301642161813	53.349596855614664	16.8386154561378
18	13.191532324337524	15.820306079802396	52.107057492099116	18.88110410376096
19	14.069433813412473	18.36948405971327	48.47194894600166	19.089133180872594
20	16.573296204291125	17.040305046700883	54.151424506107034	12.234974242900948
21	15.898962672164018	22.349038041615206	48.953984719489455	12.798014566731315
22	13.68155115496053	14.621438734732402	51.96500603425201	19.732004076055055
23	14.704321651459725	21.732464275819318	49.81944203126541	13.743772041455546
24	16.085390535850973	16.36573671877568	48.406440918333324	19.142431827040024
25	12.917290832163268	24.85172645350339	46.61964489483496	15.611337819498383
26	14.047832599987792	15.695394715216176	51.47803954900423	18.778733135791803
27	16.98442364675442	18.784368234946065	46.66707364605003	17.564134472249485
28	12.343684697418656	17.98888007100225	53.03379650717771	16.63363872440139
29	20.266634108315998	14.797535583303201	47.17798930270344	17.757841005677363
30	16.528685002653194	20.457757887964835	49.75604716577992	13.25750994360205
31	18.5357194847641	15.595606501026058	47.151457377518774	18.71721663669107
32	20.035829838789205	20.870294105216693	42.88146099337406	16.21241506262004
33	16.852233612427273	17.589727214241773	44.02069020572808	21.537348967602878
34	24.565040784030128	19.72261224413128	40.26348784462153	15.44885912721706
35	18.553563965419276	24.11705040126602	38.7227578175261	18.60662781578861
36	20.25771186798841	23.60190842024691	40.02141337678621	16.118966334978467
37	20.45846227535912	22.01961953688877	38.85541744344943	18.66650074430268
38	17.899892463524473	23.108132856854393	41.28555395372644	17.706420725894688
39	23.210269029025458	22.44859146000723	35.17616728731023	19.164972223657085
40	17.859507586252235	24.733624167061908	40.31279496222135	17.0940732844645
41	20.20112608064766	27.267775215894734	34.87257632037417	17.658522383083433
42	16.853407591417742	25.413827594141374	39.79694859380796	17.935816220632915
43	19.95482528844664	24.676099196528778	34.90356936572263	20.46550614930195
44	21.71156744978892	22.066109104911458	36.00969237054534	20.212631074754288
45	16.88487022836239	22.54579692041831	36.731924245483704	23.83740860573559
46	22.492733070048978	24.953627829876357	33.49855130992576	19.05508779014891
47	15.634347807711633	21.379800987081534	44.56917319007659	18.41667801513024
48	20.68222267094308	23.260045738221468	34.474832238402264	21.582899352433188
49	16.99639823245723	22.040985954515357	40.65606641903536	20.306549393992047
50	19.333790402486958	20.88085991613094	37.70257007480594	22.08277960657616
51	18.78507262234035	20.771914665815142	35.93338373616466	24.50962897567985
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	694.5
2	1295.0
3	15700.0
4	30105.0
5	22357.0
6	14609.0
7	14160.0
8	13711.0
9	13553.5
10	13396.0
11	12585.0
12	11774.0
13	11045.5
14	10317.0
15	9362.5
16	8408.0
17	7841.5
18	7275.0
19	6751.5
20	6228.0
21	5686.5
22	5145.0
23	5001.0
24	4857.0
25	4716.5
26	4505.5
27	4435.0
28	4802.5
29	5170.0
30	5634.0
31	6098.0
32	6534.0
33	6970.0
34	7464.5
35	7959.0
36	8546.5
37	9134.0
38	9559.0
39	9984.0
40	10528.0
41	11072.0
42	12063.5
43	13055.0
44	14512.0
45	15969.0
46	41198.5
47	66428.0
48	45975.5
49	25523.0
50	23794.5
51	22066.0
52	19439.5
53	16813.0
54	15560.0
55	14307.0
56	14043.5
57	13780.0
58	12597.5
59	11415.0
60	10372.5
61	9330.0
62	8526.0
63	7722.0
64	6834.5
65	5947.0
66	4788.5
67	3630.0
68	3054.5
69	2479.0
70	2095.5
71	1712.0
72	1468.0
73	1224.0
74	1002.0
75	646.0
76	512.0
77	407.0
78	302.0
79	231.0
80	160.0
81	116.5
82	73.0
83	54.0
84	35.0
85	24.5
86	14.0
87	12.0
88	10.0
89	6.0
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	425902.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.56486505337669
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.61356870853523	36.86178702471954
2	5.18544315906894	4.518062629335873
3	2.043933724446208	2.67131090650634
4	1.238055711421623	2.1574291998658115
5	0.9585442880921933	2.0879426279210715
6	0.7895773665927588	2.0638698854888444
7	0.7057847863079153	2.152319328056145
8	0.6022281010012709	2.098878876117734
9	0.5330265824291935	2.0899108020051345
>10	3.2143606897731205	22.24415170413434
>50	0.07516891397054769	2.204031357601655
>100	0.03268213650893378	2.6309319187908637
>500	0.0016341068254466886	0.4494439482447818
>1k	0.0038129159260422733	3.7910858063139568
>5k	0.0	0.0
>10k+	0.0021788091005955847	11.978843984897882
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14126	3.316725443881456	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	13098	3.0753553634404156	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	11679	2.742180125944466	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	11577	2.7182309545388375	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTCTGAAT	2835	0.6656460875976163	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	2810	0.6597761926452564	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTC	2486	0.5837023540626717	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTA	2400	0.5635099154265535	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	2215	0.5200726927790901	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	2161	0.5073937196819925	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGT	1069	0.2509967081629107	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTT	720	0.16905297462796606	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATG	596	0.1399382956642608	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTAT	578	0.13571197129856163	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	490	0.11504994106625469	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	473	0.11105841249864992	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0781870007654343	0.0
2	0.0	0.0	0.0	0.6792642438870914	0.0
3	0.0	0.0	0.0	0.7788176622791159	0.0
4	0.0	0.0	0.0	1.5710186850496124	0.0
5	0.0	0.0	0.0	4.720099929091669	0.0
6	0.0	0.0	0.0	4.985653976736432	0.0
7	0.0	0.0	0.0	5.646604148372161	0.0
8	0.0	0.0	0.0	6.389263257744739	0.0
9	0.0	0.0	0.0	6.513939826532864	0.0
10	0.0	0.0	0.0	10.302370028785965	0.0
11	0.0	0.0	0.0	10.547966433592704	0.0
12	0.0	0.0	0.0	13.903902775755926	0.0
13	0.0	0.0	0.0	14.180961817507313	0.0
14	0.0	0.0	0.0	14.383590591262779	0.0
15	0.0	0.0	0.0	15.114275114932543	0.0
16	0.0	0.0	0.0	15.49699226582641	0.0
17	0.0	0.0	0.0	15.66299289507915	0.0
18	0.0	0.0	0.0	15.793069767223447	0.0
19	0.0	0.0	0.0	16.729435410023903	0.0
20	0.0	0.0	0.0	16.923846330846064	0.0
21	0.0	0.0	0.0	17.07200247944363	0.0
22	0.0	0.0	0.0	17.352113866570242	0.0
23	0.0	0.0	0.0	17.4880606336669	0.0
24	0.0	0.0	0.0	17.61297199825312	0.0
25	0.0	0.0	0.0	17.713464599837522	0.0
26	0.0	0.0	0.0	17.84213269719325	0.0
27	0.0	0.0	0.0	18.02104709534118	0.0
28	0.0	0.0	0.0	18.1208353095313	0.0
29	0.0	0.0	0.0	18.235885250597555	0.0
30	0.0	0.0	0.0	18.350230804269525	0.0
31	0.0	0.0	0.0	18.468098294912913	0.0
32	0.0	0.0	0.0	18.582913440181073	0.0
33	0.0	0.0	0.0	18.704068071997785	0.0
34	0.0	0.0	0.0	18.889321956694264	0.0
35	0.0	0.0	0.0	19.03653892209945	0.0
36	0.0	0.0	0.0	19.175538034571332	0.0
37	0.0	0.0	0.0	19.331677240304106	0.0
38	0.0	0.0	0.0	19.5037825603073	0.0
39	0.0	0.0	0.0	19.685984099628552	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.162271E-6	45.000004	6
GATACCG	20	7.02828E-4	45.000004	27
CGACGAA	20	7.02828E-4	45.000004	19
GGCAACG	20	7.02828E-4	45.000004	8
ATCCGGC	20	7.02828E-4	45.000004	6
ACCGTCT	20	7.02828E-4	45.000004	40
TTCGAAA	20	7.02828E-4	45.000004	33
TTCTCCG	20	7.02828E-4	45.000004	1
TCAAGCG	65	0.0	44.999996	17
AACACGT	65	0.0	44.999996	41
CGTTTTT	11820	0.0	44.219543	1
TGATACC	1525	0.0	42.934425	4
GATACCT	1535	0.0	42.801304	5
TACGGCT	1480	0.0	42.56757	7
CACCGGT	90	0.0	42.5	16
ACGGCTG	1490	0.0	42.28188	8
ACCGAGG	70	0.0	41.785717	17
AGACACG	70	0.0	41.785717	24
CGATGAA	350	0.0	41.785713	19
CGGCTGT	1525	0.0	41.60656	9
>>END_MODULE