##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933239.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277782
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.465778920160414	31.0	27.0	31.0	16.0	33.0
2	28.639307082532344	31.0	30.0	33.0	16.0	34.0
3	28.758015278167772	31.0	30.0	33.0	16.0	34.0
4	31.9618369800779	35.0	30.0	37.0	19.0	37.0
5	33.67514453780303	35.0	33.0	37.0	28.0	37.0
6	32.61147950551152	35.0	33.0	37.0	17.0	37.0
7	32.30366258432872	35.0	32.0	35.0	26.0	37.0
8	32.81283884484956	35.0	32.0	36.0	26.0	37.0
9	33.16332231750077	35.0	32.0	37.0	25.0	39.0
10	31.65400565911398	35.0	28.0	39.0	15.0	39.0
11	32.52930715453125	35.0	30.0	39.0	17.0	39.0
12	33.26904191056296	35.0	31.0	39.0	23.0	39.0
13	33.54117617412216	35.0	32.0	39.0	25.0	39.0
14	33.68450799547847	37.0	32.0	40.0	19.0	41.0
15	34.29254955324679	37.0	32.0	40.0	25.0	41.0
16	34.44841278412568	37.0	32.0	40.0	25.0	41.0
17	32.37148195347431	35.0	27.0	40.0	18.0	41.0
18	33.05642194238648	36.0	30.0	39.0	23.0	39.0
19	32.83235774816223	36.0	31.0	37.0	21.0	39.0
20	32.271018280522135	34.0	30.0	36.0	24.0	39.0
21	32.67677171306996	35.0	31.0	37.0	24.0	39.0
22	32.75027899575927	34.0	30.0	37.0	25.0	39.0
23	33.751711773981036	35.0	32.0	38.0	27.0	40.0
24	32.15533043897733	35.0	31.0	37.0	21.0	39.0
25	31.374113513474594	34.0	29.0	37.0	18.0	39.0
26	30.353043033745887	33.0	25.0	37.0	18.0	39.0
27	32.14287822825093	34.0	30.0	37.0	22.0	39.0
28	32.168736635203146	35.0	31.0	37.0	21.0	39.0
29	32.31019288506815	35.0	30.0	38.0	21.0	40.0
30	31.393942012081418	34.0	29.0	37.0	18.0	40.0
31	31.70724164992692	35.0	30.0	37.0	20.0	39.0
32	31.53833581729558	35.0	30.0	37.0	18.0	39.0
33	31.411088551454018	35.0	30.0	38.0	15.0	40.0
34	30.46216817504374	35.0	27.0	38.0	10.0	40.0
35	29.87396951566336	35.0	25.0	38.0	8.0	40.0
36	29.1372875132298	34.0	23.0	37.0	7.0	40.0
37	29.228027733978443	35.0	23.0	38.0	7.0	40.0
38	29.005219920657208	34.0	22.0	38.0	7.0	40.0
39	28.701208141636247	34.0	22.0	37.0	7.0	40.0
40	28.40649862122096	33.0	21.0	37.0	7.0	40.0
41	27.899179932465028	33.0	20.0	37.0	7.0	39.0
42	28.340961617383417	33.0	21.0	37.0	7.0	40.0
43	28.28566285792456	33.0	20.0	37.0	7.0	40.0
44	28.25277735778416	34.0	20.0	37.0	7.0	40.0
45	28.353550626030483	34.0	20.0	37.0	7.0	40.0
46	28.170277411783342	34.0	20.0	37.0	7.0	40.0
47	28.097839312842446	34.0	20.0	37.0	7.0	40.0
48	27.898135948333586	33.0	20.0	37.0	7.0	40.0
49	27.73774758623669	33.0	19.0	37.0	7.0	39.0
50	27.87858464551339	33.0	20.0	37.0	7.0	40.0
51	26.347398319545544	31.0	18.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	13.0
11	11.0
12	30.0
13	34.0
14	63.0
15	149.0
16	365.0
17	600.0
18	1061.0
19	1695.0
20	2452.0
21	3606.0
22	4885.0
23	6569.0
24	9204.0
25	12245.0
26	15688.0
27	17016.0
28	16658.0
29	16835.0
30	18053.0
31	19774.0
32	22101.0
33	24063.0
34	26330.0
35	28386.0
36	18650.0
37	9258.0
38	1953.0
39	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.358540150189718	2.3295245912262135	31.724877781857714	43.58705747672636
2	43.5712177174907	12.83236494805279	31.637039117005422	11.959378217451095
3	11.505785112066297	12.889604078018014	63.157080012383815	12.447530797531877
4	10.525160017567734	2.543001346379535	72.45177873296326	14.480059903089474
5	18.618916992461713	3.0527535981453084	63.40691621487353	14.921413194519442
6	12.35249224211792	11.427666299472248	65.46032500305995	10.759516455349878
7	47.706834856110184	1.2542209358417753	48.233146856167785	2.8057973518802513
8	48.55462196974606	8.80834611313908	38.99388729291315	3.643144624201712
9	45.501148382544585	2.3399644325406257	38.70265171969386	13.456235465220928
10	28.534246279456553	14.142025041219371	44.39164524699225	12.932083432331828
11	21.873987515389768	14.441540488584574	50.592190998696815	13.092280997328839
12	17.025941205693673	13.100200876946671	53.359468936072176	16.514388981287485
13	15.225248576221642	14.459540214988731	59.19786019252508	11.117351016264552
14	13.378836641683044	17.785529659949166	52.541921362795286	16.2937123355725
15	10.46108099156893	14.202504121937345	60.96075339654837	14.375661489945351
16	11.981697878192252	13.789230403697864	52.83567689771116	21.393394820398733
17	12.818325161457548	14.644217407895399	55.66559388297298	16.871863547674078
18	12.672167383055777	14.127985254624129	55.33223894996796	17.867608412352133
19	14.042306556940334	17.064460620198574	51.48317745570268	17.41005536715842
20	15.763800390234067	15.951717533893483	56.150146517772924	12.134335558099517
21	15.741480729492913	19.40982497066045	51.40325867046821	13.445435629378434
22	13.314397621156157	14.823494682880819	53.38322857492565	18.478879121037362
23	13.980027503581946	20.14637377511862	51.411178550086035	14.462420171213397
24	14.451980329898987	15.208688827929814	51.9875297895472	18.351801052624
25	12.624288110820716	20.358410552159604	50.341994801679014	16.67530653534066
26	13.353277030189142	15.986996997645637	53.711543584537516	16.948182387627707
27	14.514619377785456	17.655931629839227	50.78622804933365	17.043220943041664
28	11.835900094318566	16.793384740551947	55.77935215384726	15.591363011282228
29	17.629652029289154	14.09522575256856	51.22146143378621	17.05366078435608
30	15.01177182106832	18.582197550597233	52.92171558992303	13.484315038411415
31	16.976261960818196	15.688201539336601	50.005399917921245	17.330136581923956
32	18.102324844662363	19.602422043184944	47.09664413100921	15.198608981143485
33	15.516124154912845	17.212418371240755	47.36951998329626	19.901937490550143
34	21.373235126826074	19.187348352305044	43.971531632719184	15.4678848881497
35	17.3013370196773	22.098984095441747	42.65683161615944	17.942847268721515
36	18.977471542432557	21.16407830600975	43.85093346581132	16.007516685746374
37	18.64123665320287	21.10611918698836	42.27991734525635	17.97272681455242
38	16.693306261744823	21.644671001000784	44.903197471398435	16.758825265855958
39	20.692485474220792	21.31131606799577	39.849234291638766	18.146964166144674
40	17.579612789885594	22.891332051752812	43.187463550554035	16.341591607807562
41	18.461599383689368	24.960580599174893	39.532799101453655	17.045020915682084
42	16.23251326579836	23.711759581254366	42.37243593897373	17.683291213973547
43	18.29528191171494	22.68541518168924	39.72359620133774	19.29570670525808
44	20.529767947527198	20.81596359735332	39.89171364595258	18.7625548091669
45	16.700506152306485	21.281796516692946	40.54834366517629	21.46935366582428
46	19.902297485078225	23.497562837044878	37.719866658026795	18.8802730198501
47	15.593522978450727	20.50600830867371	45.850343074785265	18.0501256380903
48	19.69422064784615	21.447034005083125	38.63533274294231	20.22341260412842
49	16.353831421762386	20.902362284093282	42.33967643691816	20.40412985722617
50	17.920887602508444	20.050615230648493	40.55194361045712	21.476553556385944
51	17.77796977485942	19.97645635786336	39.365401645894984	22.880172221382235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	266.5
2	464.0
3	16216.5
4	31969.0
5	21102.5
6	10236.0
7	9764.5
8	9293.0
9	9210.0
10	9127.0
11	8507.0
12	7887.0
13	7240.5
14	6594.0
15	5891.0
16	5188.0
17	4774.5
18	4361.0
19	4027.0
20	3693.0
21	3359.5
22	3026.0
23	2905.0
24	2784.0
25	2751.5
26	2586.5
27	2454.0
28	2669.5
29	2885.0
30	3140.5
31	3396.0
32	3664.0
33	3932.0
34	4242.5
35	4553.0
36	4952.5
37	5352.0
38	5691.0
39	6030.0
40	6488.5
41	6947.0
42	7769.0
43	8591.0
44	9717.5
45	10844.0
46	20193.0
47	29542.0
48	24442.0
49	19342.0
50	18083.5
51	16825.0
52	14345.5
53	11866.0
54	10717.5
55	9569.0
56	9037.5
57	8506.0
58	7814.0
59	7122.0
60	6534.5
61	5947.0
62	5451.0
63	4955.0
64	4454.5
65	3954.0
66	3245.5
67	2537.0
68	2131.5
69	1726.0
70	1474.0
71	1222.0
72	1038.5
73	855.0
74	713.0
75	462.0
76	353.0
77	297.5
78	242.0
79	182.5
80	123.0
81	94.0
82	65.0
83	45.5
84	26.0
85	26.0
86	26.0
87	19.0
88	12.0
89	6.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	277782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.271765960068535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55179135291928	31.23147591148832
2	8.325927087429287	6.705995741310947
3	3.405814697095952	4.114745171544296
4	2.266539454952996	3.651101858765134
5	1.683830695948733	3.390541785181335
6	1.2679397756747364	3.0637304338460862
7	1.0340579247621582	2.915033711462305
8	0.7735208292151142	2.4920839839511375
9	0.6472449264644062	2.34591265776747
>10	2.9210431817391442	17.911195079991685
>50	0.06760752047381492	1.899065283559256
>100	0.04474027090178929	3.4826206697023845
>500	0.0029826847267859524	0.8344195764152413
>1k	0.004971141211309921	4.524043567138106
>5k	9.942282422619841E-4	2.414771816391709
>10k+	9.942282422619841E-4	9.023262751484587
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22536	8.112836684882389	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	6031	2.1711269988696174	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	4618	1.6624547306880935	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	3255	1.1717821889107285	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTCTGAAT	1302	0.4687128755642914	No Hit
GCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGC	1082	0.3895140793859933	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	1042	0.3751142982626664	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTC	822	0.29591550208436834	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTA	758	0.2728758522870452	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	504	0.18143724215391926	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGGTCTGAATCGTATGCCG	480	0.1727973734799231	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	388	0.13967787689627117	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGT	379	0.1364379261435226	No Hit
GAATCTTTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	355	0.12779805746952647	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	347	0.12491810124486108	No Hit
CTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCTGCT	296	0.10655838031261924	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCT	285	0.10259844050370434	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13067801369419185	0.0
2	0.0	0.0	0.0	0.7667883448171587	0.0
3	0.0	0.0	0.0	0.9539854994204088	0.0
4	0.0	0.0	0.0	1.619615381846196	0.0
5	0.0	0.0	0.0	3.237790785580059	0.0
6	0.0	0.0	0.0	3.547386079731588	0.0
7	0.0	0.0	0.0	3.942660071566912	0.0
8	0.0	0.0	0.0	4.523691239893154	0.0
9	0.0	0.0	0.0	4.703328509406657	0.0
10	0.0	0.0	0.0	7.88028021974066	0.0
11	0.0	0.0	0.0	8.584069522143263	0.0
12	0.0	0.0	0.0	11.003232750862187	0.0
13	0.0	0.0	0.0	11.2725086578684	0.0
14	0.0	0.0	0.0	11.419386425326335	0.0
15	0.0	0.0	0.0	11.924818742755111	0.0
16	0.0	0.0	0.0	12.260333642928627	0.0
17	0.0	0.0	0.0	12.551209221619832	0.0
18	0.0	0.0	0.0	12.83920484408637	0.0
19	0.0	0.0	0.0	13.681592039800995	0.0
20	0.0	0.0	0.0	13.984347437918943	0.0
21	0.0	0.0	0.0	14.310862474890381	0.0
22	0.0	0.0	0.0	14.799735044027331	0.0
23	0.0	0.0	0.0	15.12877004269535	0.0
24	0.0	0.0	0.0	15.37608628348849	0.0
25	0.0	0.0	0.0	15.581643159023983	0.0
26	0.0	0.0	0.0	15.781440122110144	0.0
27	0.0	0.0	0.0	15.988076981229886	0.0
28	0.0	0.0	0.0	16.174194152248887	0.0
29	0.0	0.0	0.0	16.367511213829548	0.0
30	0.0	0.0	0.0	16.571268116724625	0.0
31	0.0	0.0	0.0	16.766385150945705	0.0
32	0.0	0.0	0.0	16.975181977233945	0.0
33	0.0	0.0	0.0	17.158779186556366	0.0
34	0.0	0.0	0.0	17.360016127754857	0.0
35	0.0	0.0	0.0	17.57925279535751	0.0
36	0.0	0.0	0.0	17.778329769387504	0.0
37	0.0	0.0	0.0	17.969846858327752	0.0
38	0.0	0.0	0.0	18.195563427435903	0.0
39	0.0	0.0	0.0	18.45043955331879	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGCAC	45	3.8198777E-10	45.000004	6
ACCCGGT	45	3.8198777E-10	45.000004	39
CACCGGT	90	0.0	45.000004	16
GTTATAC	30	2.1595388E-6	45.000004	33
CTTGTCG	20	7.024041E-4	45.0	1
CATGGGT	20	7.024041E-4	45.0	4
CCGATGA	95	0.0	45.0	18
GACACGA	25	3.8831437E-5	45.0	25
ACTCTGA	20	7.024041E-4	45.0	42
GTCGTTT	20	7.024041E-4	45.0	9
CGGTGTA	25	3.8831437E-5	45.0	19
CACACCG	25	3.8831437E-5	45.0	17
TCATACT	25	3.8831437E-5	45.0	40
GACTGAT	25	3.8831437E-5	45.0	1
CCACCGG	100	0.0	45.0	15
TGCTACG	65	0.0	44.999996	4
CGTTTTT	9180	0.0	44.534313	1
TACGGCT	600	0.0	44.25	7
CGACCAC	530	0.0	43.301888	12
ACGGCTG	615	0.0	43.17073	8
>>END_MODULE