FastQCFastQC Report
Sat 14 Jan 2017
SRR2933234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549373
Sequences flagged as poor quality0
Sequence length51
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248974.531893631467145No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC163592.977758280803753Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG146382.6644920664102534No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC108021.9662415153274733No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT38540.7015270135226885Illumina PCR Primer Index 7 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC27480.5002065991594054Illumina PCR Primer Index 7 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC25310.46070702418939413Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT21530.39190131295131No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT21200.3858944651448106Illumina PCR Primer Index 7 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT19170.34894324985028385Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA17050.3103538033358028No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGT9800.17838517728392186No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA7210.1312405232874568No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7090.1290562149941843No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC7090.1290562149941843No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA6850.12468759840763925No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA6530.1188627762922459No Hit
GCGCTCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC6500.11831669921892776No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACA207.0300663E-445.00000418
TCGATTG253.8881375E-545.01
TACGGGA351.2103737E-745.04
CGTTGAT302.1634241E-644.99999625
TCGGGTA302.1634241E-644.9999965
CGTTCAT302.1634241E-644.99999617
CGTTTTT182400.044.4078941
GATACCT16150.043.0495345
TCGTTTG1100.042.9545441
TGATACC16150.042.9102174
ATACCTG16500.042.409096
GTTGATC750.042.016
TACCTGT17200.041.3372087
AATCTGT19650.041.3358762
ATGACTG2450.041.326533
ACGGCTG12000.041.258
GAATCTG19850.041.14611
TACGGCT12100.040.9090927
ACATACG556.002665E-1140.90909217
TACGAAA501.0786607E-940.520
Produced by FastQC (version 0.11.5)