##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933234.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	549373
Sequences flagged as poor quality	0
Sequence length	51
%GC	36
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.59057143325209	31.0	28.0	33.0	25.0	34.0
2	28.942476241096667	31.0	28.0	33.0	16.0	34.0
3	28.83744741732848	31.0	28.0	34.0	16.0	34.0
4	33.3242278015119	35.0	32.0	37.0	28.0	37.0
5	34.32594430377903	35.0	33.0	37.0	30.0	37.0
6	33.87349032442439	35.0	33.0	37.0	28.0	37.0
7	33.845653135483545	35.0	33.0	37.0	29.0	37.0
8	33.72514848745752	35.0	33.0	37.0	28.0	37.0
9	34.941007293769445	37.0	34.0	39.0	28.0	39.0
10	34.80027413069081	37.0	33.0	39.0	27.0	39.0
11	34.95615911229711	37.0	34.0	39.0	27.0	39.0
12	35.060427068676475	37.0	34.0	39.0	27.0	39.0
13	35.12312399772104	37.0	34.0	39.0	27.0	39.0
14	36.06457179366296	38.0	34.0	40.0	27.0	41.0
15	36.24439861442044	38.0	34.0	41.0	27.0	41.0
16	36.23031710695648	38.0	34.0	41.0	29.0	41.0
17	35.55221679987913	38.0	33.0	40.0	27.0	41.0
18	35.281824916768755	38.0	33.0	39.0	27.0	40.0
19	34.418808350610604	37.0	33.0	37.0	27.0	39.0
20	33.58719121616825	35.0	32.0	37.0	27.0	39.0
21	33.973631758386375	35.0	33.0	37.0	27.0	39.0
22	34.44255360201539	35.0	33.0	38.0	29.0	40.0
23	34.83525582800757	35.0	33.0	38.0	30.0	40.0
24	34.30464001689199	35.0	33.0	38.0	27.0	40.0
25	33.38409969183051	35.0	33.0	38.0	24.0	40.0
26	33.2492969257681	35.0	32.0	38.0	24.0	40.0
27	34.02270406445166	35.0	33.0	38.0	26.0	40.0
28	33.78950367054806	35.0	33.0	38.0	24.0	40.0
29	33.67167844069512	35.0	33.0	38.0	24.0	40.0
30	32.99599907530949	35.0	33.0	38.0	21.0	40.0
31	32.74038585806001	35.0	32.0	38.0	21.0	40.0
32	32.07392063315817	35.0	31.0	38.0	16.0	40.0
33	31.226587400545714	35.0	30.0	38.0	12.0	40.0
34	30.4014121553116	35.0	25.0	38.0	8.0	40.0
35	29.940621399304298	35.0	23.0	39.0	7.0	40.0
36	29.21061282589425	35.0	21.0	39.0	7.0	40.0
37	29.086256514244422	35.0	20.0	39.0	7.0	40.0
38	28.772462425346713	35.0	18.0	38.0	7.0	40.0
39	28.307350379432553	34.0	18.0	38.0	7.0	40.0
40	28.118709874711715	34.0	18.0	38.0	7.0	40.0
41	27.48633078072639	33.0	16.0	38.0	7.0	40.0
42	27.63436135376147	33.0	15.0	38.0	7.0	40.0
43	27.504891940448474	33.0	15.0	38.0	7.0	40.0
44	27.51566422084813	34.0	15.0	38.0	7.0	40.0
45	27.58118436836175	34.0	15.0	38.0	7.0	40.0
46	27.37459977101168	34.0	15.0	38.0	7.0	40.0
47	27.273042177172886	33.0	15.0	37.0	7.0	40.0
48	27.180028869274608	33.0	15.0	37.0	7.0	40.0
49	27.369752426857527	34.0	15.0	38.0	7.0	40.0
50	27.35570004350414	34.0	15.0	37.0	7.0	40.0
51	26.20016637148167	32.0	15.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	11.0
9	27.0
10	41.0
11	38.0
12	44.0
13	68.0
14	97.0
15	155.0
16	298.0
17	688.0
18	1185.0
19	2193.0
20	3519.0
21	5330.0
22	7851.0
23	11333.0
24	16700.0
25	24338.0
26	32183.0
27	34287.0
28	31743.0
29	28876.0
30	28312.0
31	30228.0
32	33958.0
33	38207.0
34	41363.0
35	48030.0
36	48691.0
37	44790.0
38	30574.0
39	4211.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.10953395962306	2.126424123500791	27.270542964434004	45.493498952442145
2	43.834698829392785	15.947998900564825	27.07613952633275	13.141162743709648
3	10.6576042142588	16.256350421298464	59.93960387569102	13.146441488751723
4	9.269294268193013	2.594594201025533	73.21218916837923	14.923922362402228
5	19.388648513851244	2.8170295955571167	61.892011438494436	15.90231045209721
6	11.928325563870084	12.81952334752527	64.5657868151511	10.686364273453556
7	45.26141619628194	1.0906979411074078	51.12919637477633	2.5186894878343127
8	44.081889717914784	9.654824681955612	42.975355541681154	3.2879300584484494
9	41.59305244342186	2.0923853192639608	41.86900339113863	14.44555884617555
10	27.971887952265583	13.303347634485133	47.17796469793747	11.546799715311819
11	23.386478767613262	12.692833466515463	51.14976527787132	12.770922487999956
12	17.66450116769481	12.255971807860961	54.263496749931285	15.816030274512945
13	16.138943850535064	12.870854592417174	59.95653226496388	11.033669292083886
14	13.602779896354573	17.591144814179074	53.92492896447405	14.881146324992308
15	11.022565724926416	12.085959812367918	62.368008620736724	14.523465841968935
16	14.108265240556053	15.115231363754681	51.50125688739708	19.27524650829218
17	14.16760561585662	15.361330098130049	56.00384438259616	14.467219903417169
18	15.449430532625374	13.693246664834275	54.515784357804264	16.341538444736088
19	14.10881131762937	16.745817504682613	51.18052761966824	17.96484355801978
20	16.127476231995384	16.88743349236311	55.90172796988567	11.083362305755834
21	14.809064151314317	20.519756158384194	53.38831722709343	11.282862463208057
22	13.850698887641002	11.880088755726984	56.135266931574726	18.133945425057295
23	14.168697770003257	18.546415641103586	55.169438614566054	12.115447974327097
24	16.339536162133925	14.619757432564032	52.42703955236242	16.613666852939623
25	14.10244041844066	22.60504247569502	49.18006527441283	14.112451831451493
26	14.006330853536669	13.281686577243512	53.93803481423368	18.77394775498614
27	17.0346922764679	14.370018184366543	50.99995813409105	17.595331405074514
28	11.202589133430292	17.696901740711684	53.86213010104246	17.23837902481556
29	17.52834595074749	17.78500217520701	49.28345586696106	15.403196007084441
30	17.91678877556778	17.751691473734603	51.89406832880393	12.437451421893686
31	18.48179652076094	17.69835794624053	47.58606629739722	16.23377923560131
32	22.553893256494224	17.493214992364024	46.56999888964328	13.38289286149847
33	17.10932280982138	16.235781518203478	42.74127050291878	23.91362516905636
34	17.313919686624573	25.04618901911816	44.160524816472595	13.479366477784675
35	18.56716656988967	20.70196387518134	43.89804376989768	16.8328257850313
36	17.195967038787856	27.05211213510675	37.07044212220112	18.68147870390427
37	22.800902119325123	21.531090898169367	39.431133310155396	16.236873672350114
38	19.72830845345512	23.765820307878254	36.31503550411105	20.190835734555577
39	23.39721828338852	22.872438215929797	35.69105143499954	18.03929206568215
40	21.569862370374953	19.903053116916922	41.02130974765779	17.50577476505034
41	18.67820224146436	25.25624666665453	34.759807999301024	21.305743092580087
42	19.3360430891216	20.936959042399245	42.716697034619465	17.01030083385969
43	21.610636125182708	19.623279629686934	35.96044945783648	22.80563478729388
44	23.41505680111691	20.411086820793887	35.419287078178215	20.754569299910987
45	19.282345510245317	20.13549992445934	36.46848316171344	24.1136714035819
46	22.916670458868566	22.093732309378144	34.72631527213751	20.26328195961578
47	16.195007763395726	20.934046631341545	43.50632448263748	19.36462112262525
48	18.92739541258853	24.140247154483383	35.02611158538917	21.906245847538923
49	18.290305493717383	19.970402622626157	41.07591745498959	20.663374428666863
50	17.997244131036656	19.929992919200615	38.48751212746167	23.58525082230106
51	18.17362702571841	19.887216881790696	36.28700354768072	25.652152544810175
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	76.0
1	1147.0
2	2218.0
3	26880.5
4	51543.0
5	36762.5
6	21982.0
7	20572.5
8	19163.0
9	18492.5
10	17822.0
11	16616.0
12	15410.0
13	14215.5
14	13021.0
15	12020.0
16	11019.0
17	10286.0
18	9553.0
19	8798.0
20	8043.0
21	7519.0
22	6995.0
23	6640.0
24	6285.0
25	6098.5
26	6045.0
27	6178.0
28	6324.0
29	6470.0
30	6818.0
31	7166.0
32	7430.0
33	7694.0
34	8411.0
35	9128.0
36	9327.0
37	9526.0
38	9720.5
39	9915.0
40	10243.0
41	10571.0
42	10936.0
43	11301.0
44	13221.0
45	15141.0
46	44591.0
47	74041.0
48	50718.5
49	27396.0
50	26163.5
51	24931.0
52	23249.5
53	21568.0
54	21612.0
55	21656.0
56	20924.5
57	20193.0
58	19288.0
59	18383.0
60	16486.0
61	14589.0
62	13228.5
63	11868.0
64	10605.0
65	9342.0
66	8104.5
67	6867.0
68	6064.5
69	5262.0
70	4444.0
71	3626.0
72	3162.0
73	2698.0
74	2357.0
75	1721.0
76	1426.0
77	1059.0
78	692.0
79	521.0
80	350.0
81	274.5
82	199.0
83	137.0
84	75.0
85	54.0
86	33.0
87	24.0
88	15.0
89	10.5
90	6.0
91	5.0
92	4.0
93	2.0
94	0.0
95	2.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	549373.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.79339184796633
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.95393543831496	44.085801625162105
2	9.651271014320727	10.383492070085486
3	4.002574745966355	6.459362151316272
4	1.832780788087555	3.943659804200735
5	0.8994847120458104	2.419316678816772
6	0.4594862357243901	1.4830393876241486
7	0.25682333684950975	0.9670778876393551
8	0.16621807960596435	0.7153147430768081
9	0.11491118456180764	0.5563316140962566
>10	0.5487489230490149	5.49012681084158
>50	0.060309443764632896	2.340652308620132
>100	0.04660275184918125	4.589507090888906
>500	0.003084005637078171	1.1332753472080084
>1k	0.0023986710510608	3.138811420341244
>5k	0.0	0.0
>10k+	0.0013706691720347427	12.294231060082195
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24897	4.531893631467145	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	16359	2.977758280803753	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	14638	2.6644920664102534	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	10802	1.9662415153274733	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	3854	0.7015270135226885	Illumina PCR Primer Index 7 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC	2748	0.5002065991594054	Illumina PCR Primer Index 7 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	2531	0.46070702418939413	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	2153	0.39190131295131	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	2120	0.3858944651448106	Illumina PCR Primer Index 7 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	1917	0.34894324985028385	Illumina PCR Primer Index 7 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA	1705	0.3103538033358028	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGT	980	0.17838517728392186	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	721	0.1312405232874568	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	709	0.1290562149941843	No Hit
GCGCGCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	709	0.1290562149941843	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	685	0.12468759840763925	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA	653	0.1188627762922459	No Hit
GCGCTCGGGAGAAACAAGCGAGATAGGAATGTCTTACACGCGGGGCAAGAC	650	0.11831669921892776	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04532439708540464	0.0
2	0.0	0.0	0.0	0.5759292866595191	0.0
3	0.0	0.0	0.0	0.6875110353075233	0.0
4	0.0	0.0	0.0	1.5379350641549547	0.0
5	0.0	0.0	0.0	5.02063261208687	0.0
6	0.0	0.0	0.0	5.5698041221538	0.0
7	0.0	0.0	0.0	6.161205592557334	0.0
8	0.0	0.0	0.0	6.896225333243534	0.0
9	0.0	0.0	0.0	7.094087259475803	0.0
10	0.0	0.0	0.0	10.414599916632234	0.0
11	0.0	0.0	0.0	10.689822761584571	0.0
12	0.0	0.0	0.0	13.136794127123101	0.0
13	0.0	0.0	0.0	13.342665183764037	0.0
14	0.0	0.0	0.0	13.505578177303944	0.0
15	0.0	0.0	0.0	13.933702602785358	0.0
16	0.0	0.0	0.0	14.197457829198013	0.0
17	0.0	0.0	0.0	14.320143145003486	0.0
18	0.0	0.0	0.0	14.400234449090144	0.0
19	0.0	0.0	0.0	14.963603963063347	0.0
20	0.0	0.0	0.0	15.089565741308729	0.0
21	0.0	0.0	0.0	15.168928942630963	0.0
22	0.0	0.0	0.0	15.338030809668476	0.0
23	0.0	0.0	0.0	15.426131244163802	0.0
24	0.0	0.0	0.0	15.508406856543733	0.0
25	0.0	0.0	0.0	15.582309287132786	0.0
26	0.0	0.0	0.0	15.680967211712261	0.0
27	0.0	0.0	0.0	15.83969361435673	0.0
28	0.0	0.0	0.0	15.917054533076799	0.0
29	0.0	0.0	0.0	16.009523584158668	0.0
30	0.0	0.0	0.0	16.115826587764598	0.0
31	0.0	0.0	0.0	16.205565253479875	0.0
32	1.820256911060427E-4	0.0	0.0	16.318967259038942	0.0
33	1.820256911060427E-4	0.0	0.0	16.42071962036722	0.0
34	1.820256911060427E-4	0.0	0.0	16.55414445194795	0.0
35	1.820256911060427E-4	0.0	0.0	16.684474846779874	0.0
36	1.820256911060427E-4	0.0	0.0	16.79278013298797	0.0
37	1.820256911060427E-4	0.0	0.0	16.92711509302423	0.0
38	1.820256911060427E-4	0.0	0.0	17.10477216754373	0.0
39	1.820256911060427E-4	0.0	0.0	17.444432107147602	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGACA	20	7.0300663E-4	45.000004	18
TCGATTG	25	3.8881375E-5	45.0	1
TACGGGA	35	1.2103737E-7	45.0	4
CGTTGAT	30	2.1634241E-6	44.999996	25
TCGGGTA	30	2.1634241E-6	44.999996	5
CGTTCAT	30	2.1634241E-6	44.999996	17
CGTTTTT	18240	0.0	44.407894	1
GATACCT	1615	0.0	43.049534	5
TCGTTTG	110	0.0	42.954544	1
TGATACC	1615	0.0	42.910217	4
ATACCTG	1650	0.0	42.40909	6
GTTGATC	75	0.0	42.0	16
TACCTGT	1720	0.0	41.337208	7
AATCTGT	1965	0.0	41.335876	2
ATGACTG	245	0.0	41.32653	3
ACGGCTG	1200	0.0	41.25	8
GAATCTG	1985	0.0	41.1461	1
TACGGCT	1210	0.0	40.909092	7
ACATACG	55	6.002665E-11	40.909092	17
TACGAAA	50	1.0786607E-9	40.5	20
>>END_MODULE