FastQCFastQC Report
Sat 14 Jan 2017
SRR2933233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434913
Sequences flagged as poor quality0
Sequence length51
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT407999.380956651100334No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG63291.4552335754507224No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC44551.0243427995944017Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC39060.8981106566140814No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT12740.2929321496483205Illumina PCR Primer Index 7 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC10430.2398180785582404Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT9280.21337600853504035No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC7720.1775067657209603Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6450.14830552317360024No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT5640.1296811086355202Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA5520.12692193611136018No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA5230.1202539358446402No Hit
GAATCTTTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC5130.11795462540784019Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTCTACACATCTGACGCAGAGCATCTCGTATGCCG4990.11473559079632019No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC4820.11082676305376017No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT4610.10599821113648016Illumina PCR Primer Index 7 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAA207.028444E-445.00000429
CTACGGC406.7975634E-945.0000046
GGCGATA207.028444E-445.0000048
TATACGG207.028444E-445.00000427
CATCGAC253.8867933E-545.022
CGATGAA1250.044.99999619
CGTTTTT165250.044.5642971
TGATACC7850.044.140134
GATACCT7850.044.140135
ATACCTG7850.043.8535046
GAATGCT1600.043.5937541
CCGATGA1450.043.4482818
ATGATCC850.042.352943
ACCACCG3150.042.14285714
ATGATAC22150.041.8510173
CGACCAC5250.041.5714312
GAATGAT24050.041.444911
GGACTAA2200.040.9090928
AATGATA22550.040.9090922
GTTTTTT181850.040.8551032
Produced by FastQC (version 0.11.5)