##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933233.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	434913
Sequences flagged as poor quality	0
Sequence length	51
%GC	35
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.877780153731894	31.0	28.0	31.0	16.0	33.0
2	29.246056107773278	31.0	30.0	33.0	16.0	34.0
3	29.362913962102766	31.0	30.0	33.0	16.0	34.0
4	32.32088026800763	35.0	32.0	37.0	19.0	37.0
5	33.668908494342546	35.0	33.0	37.0	28.0	37.0
6	33.158907643597686	35.0	33.0	37.0	26.0	37.0
7	32.26331243260146	35.0	32.0	37.0	25.0	37.0
8	33.214300331330634	35.0	33.0	37.0	28.0	37.0
9	33.19666462028038	35.0	31.0	38.0	25.0	39.0
10	31.754385359830586	35.0	27.0	39.0	15.0	39.0
11	32.42440212180367	37.0	29.0	39.0	15.0	39.0
12	33.248608342358125	37.0	31.0	39.0	22.0	39.0
13	33.399967349791794	37.0	32.0	39.0	22.0	39.0
14	33.95742826726265	37.0	32.0	40.0	21.0	41.0
15	34.33927015288115	37.0	32.0	40.0	23.0	41.0
16	34.49869973994799	37.0	32.0	40.0	25.0	41.0
17	32.673700257292836	36.0	29.0	40.0	18.0	41.0
18	33.21516717136531	36.0	30.0	39.0	23.0	39.0
19	32.776408155194254	36.0	31.0	37.0	21.0	39.0
20	32.240905652394844	34.0	30.0	35.0	24.0	39.0
21	32.47847730465633	35.0	31.0	36.0	23.0	39.0
22	32.38033813659283	34.0	30.0	37.0	23.0	39.0
23	33.57355149190758	35.0	32.0	38.0	26.0	39.0
24	31.74470526289166	34.0	30.0	36.0	21.0	39.0
25	31.09122514158004	34.0	28.0	36.0	18.0	39.0
26	29.84596689452833	33.0	24.0	36.0	18.0	39.0
27	31.75458310052815	34.0	30.0	37.0	22.0	39.0
28	31.92103478166898	35.0	30.0	37.0	21.0	39.0
29	31.93963620310269	35.0	30.0	37.0	21.0	39.0
30	31.127292125091685	34.0	28.0	37.0	18.0	39.0
31	31.334501383035228	34.0	30.0	36.0	19.0	39.0
32	31.094878745864115	35.0	30.0	36.0	17.0	39.0
33	30.79969327198773	35.0	29.0	37.0	15.0	40.0
34	29.735365463897377	34.0	25.0	37.0	10.0	39.0
35	29.042560236185167	34.0	23.0	37.0	8.0	39.0
36	28.191824571810916	33.0	21.0	37.0	7.0	39.0
37	28.253144881849934	34.0	20.0	37.0	7.0	39.0
38	28.15693483524291	33.0	20.0	37.0	7.0	39.0
39	27.619503210987023	33.0	18.0	36.0	7.0	39.0
40	27.495466909473848	33.0	18.0	36.0	7.0	39.0
41	26.959033185947533	33.0	18.0	36.0	7.0	39.0
42	27.303067510053737	33.0	18.0	36.0	7.0	39.0
43	27.272086601228292	33.0	18.0	37.0	7.0	39.0
44	27.118338610250785	33.0	17.0	36.0	7.0	39.0
45	27.279340925656395	33.0	18.0	37.0	7.0	39.0
46	26.998762970985002	33.0	17.0	36.0	7.0	39.0
47	27.02782165628528	33.0	17.0	36.0	7.0	39.0
48	26.83274815882717	33.0	17.0	36.0	7.0	39.0
49	26.642268683621783	33.0	15.0	36.0	7.0	39.0
50	26.833007980906526	33.0	17.0	36.0	7.0	39.0
51	25.444247470183694	30.0	15.0	35.0	7.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	14.0
10	24.0
11	29.0
12	42.0
13	80.0
14	159.0
15	291.0
16	603.0
17	999.0
18	1716.0
19	2803.0
20	4340.0
21	6144.0
22	8517.0
23	11531.0
24	15785.0
25	21442.0
26	27097.0
27	29045.0
28	28359.0
29	27505.0
30	29231.0
31	31323.0
32	33607.0
33	36239.0
34	38784.0
35	40350.0
36	25094.0
37	11596.0
38	2117.0
39	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.09396362031027	2.1802061561737633	30.89330509780117	48.832525125714795
2	48.433364833886316	9.059513052035694	29.91655802424853	12.59056408982946
3	11.299961141653618	9.045717189414894	66.38546100024602	13.26886066868546
4	10.165941234223856	2.7288216263942444	71.80240645830315	15.302830681078744
5	15.499421723425144	2.968869635996165	66.33993465359738	15.191773986981305
6	12.472379533377941	7.925493144605933	68.19087955522139	11.411247766794737
7	46.326506680646474	1.212656324368322	49.68947812551016	2.7713588694750446
8	47.49340672732247	5.708268090399689	43.73012533541191	3.068199846865925
9	44.210681216703115	2.134909740568803	43.61125098582935	10.043158056898736
10	26.796623692554604	13.808048966115061	48.311731311779596	11.083596029550739
11	21.116867051571234	13.469360538774422	53.28100102779176	12.132771381862579
12	17.318176278933947	12.3797173227749	55.94820113447977	14.353905263811384
13	15.208329022126266	12.99524272670626	59.709413146997214	12.08701510417026
14	13.146307422404021	13.954055178851862	57.57036464764217	15.329272751101945
15	11.694522812608499	11.997801859222418	61.5881797049065	14.719495623262583
16	14.166971325299544	12.94787693170818	54.82291860670985	18.06223313628243
17	14.171799877216824	13.622034751777942	57.551740233104084	14.654425137901145
18	14.931032183448185	12.62068505655154	56.65087040396585	15.797412356034426
19	14.378277954441462	15.767751251399705	53.59784600598281	16.256124788176027
20	15.813507529092025	15.923414567971065	56.47037453467705	11.79270336825986
21	15.508618965172344	17.356114901141147	54.492507696941686	12.642758436744819
22	14.402190782984183	12.884645894696181	56.19008859243113	16.523074729888506
23	14.448636853807542	16.729782738156825	55.315660833316095	13.505919574719542
24	14.624534102222745	14.516466511693144	54.8118819166132	16.047117469470905
25	14.621085136567544	17.75619491714435	52.17089394890473	15.451825997383384
26	13.900941107761783	14.245837673281784	54.99306757903305	16.860153639923386
27	14.765021969911224	14.00349035324306	53.96021733082249	17.271270346023226
28	12.36684118432882	16.307859273004027	55.116540549489216	16.208758993177945
29	16.527673350762107	15.621055245531865	52.25481877984792	15.596452623858104
30	16.553195696610587	16.005959812652186	53.6633763534316	13.77746813730562
31	16.813477638056344	16.606539698744346	51.01411086815064	15.565871795048663
32	19.30684987572227	16.460073623920184	49.83456461407224	14.398511886285304
33	16.52629376450003	16.14690754242803	47.34165223849367	19.985146454578274
34	16.473869486540988	22.191104887644194	46.957437464504395	14.377588161310422
35	17.498212286135388	20.884866628498113	45.50910182036407	16.107819265002426
36	17.408079317012827	23.986176545653958	41.411270759899104	17.194473377434107
37	20.645738343070914	21.144918638900194	42.04840968193639	16.160933336092505
38	18.79249413101011	21.449347340732512	41.66350511481607	18.094653413441307
39	20.39695295380915	21.514417826093954	40.53730286287143	17.55132635722547
40	19.922375279653632	19.66761168325619	43.68620850606903	16.72380453102115
41	17.72147532954867	22.149027506650757	40.65272824679879	19.47676891700179
42	17.93623092434579	20.239680119932032	44.6029435772212	17.22114537850099
43	19.17303000830051	19.147967524539393	41.23675309774599	20.44224936941411
44	21.143998914725472	20.07550935474451	40.058356498885985	18.722135231644028
45	18.19720265892259	20.088155562146913	40.593635968573025	21.121005810357474
46	19.73130258235555	21.419686236097792	39.41477950762567	19.43423167392099
47	16.330162584240988	20.621825514528194	44.22838590706647	18.819625994164348
48	18.26549217889555	21.954735774741156	39.303262951440864	20.47650909492243
49	17.236320827383867	20.182427290055713	41.82882553522199	20.75242634733843
50	17.068241234453787	19.579318162483073	41.160186060200544	22.192254542862596
51	17.62145532554787	19.13141248939443	40.54971913923014	22.697413045827556
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	596.0
2	1074.0
3	29671.0
4	58268.0
5	38279.5
6	18291.0
7	17124.5
8	15958.0
9	15457.0
10	14956.0
11	13813.5
12	12671.0
13	11574.0
14	10477.0
15	9435.5
16	8394.0
17	7687.5
18	6981.0
19	6485.0
20	5989.0
21	5396.0
22	4803.0
23	4597.0
24	4391.0
25	4232.5
26	4132.5
27	4191.0
28	4314.5
29	4438.0
30	4665.0
31	4892.0
32	5379.0
33	5866.0
34	6469.0
35	7072.0
36	7289.5
37	7507.0
38	7720.0
39	7933.0
40	8012.5
41	8092.0
42	8743.5
43	9395.0
44	10525.0
45	11655.0
46	22484.5
47	33314.0
48	28279.5
49	23245.0
50	22504.5
51	21764.0
52	20088.5
53	18413.0
54	17539.5
55	16666.0
56	15851.0
57	15036.0
58	14494.5
59	13953.0
60	12930.0
61	11907.0
62	11111.0
63	10315.0
64	9370.5
65	8426.0
66	7630.0
67	6834.0
68	6117.0
69	5400.0
70	4695.5
71	3991.0
72	3436.5
73	2882.0
74	2551.0
75	1860.0
76	1500.0
77	1153.0
78	806.0
79	608.0
80	410.0
81	305.0
82	200.0
83	136.5
84	73.0
85	56.5
86	40.0
87	28.5
88	17.0
89	11.5
90	6.0
91	6.0
92	6.0
93	3.5
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	434913.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.69257117981824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.21841691309257	44.4204404813147
2	10.131353487126836	11.082195434851686
3	4.048462942806367	6.64262543004881
4	1.9164945864192726	4.192720663338896
5	0.9499232747915118	2.5976873160950404
6	0.505833316760065	1.6599194809214044
7	0.282485154462864	1.08148875923915
8	0.17525875149284867	0.7668281392726963
9	0.10959362382661474	0.5394561364792215
>10	0.5471798772292839	5.57164406616023
>50	0.06626282958658364	2.591571152325544
>100	0.043177714762495184	4.286239837976205
>500	0.0029925148845293694	1.0514521356850859
>1k	0.001710008505445354	2.496643518978285
>5k	4.275021263613385E-4	1.479795545197
>10k+	4.275021263613385E-4	9.539291902116037
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	40799	9.380956651100334	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCG	6329	1.4552335754507224	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	4455	1.0243427995944017	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGC	3906	0.8981106566140814	No Hit
GAACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCT	1274	0.2929321496483205	Illumina PCR Primer Index 7 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTCTGC	1043	0.2398180785582404	Illumina PCR Primer Index 7 (96% over 27bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGAGCATCT	928	0.21337600853504035	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTC	772	0.1775067657209603	Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	645	0.14830552317360024	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTT	564	0.1296811086355202	Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA	552	0.12692193611136018	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTA	523	0.1202539358446402	No Hit
GAATCTTTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCTTC	513	0.11795462540784019	Illumina PCR Primer Index 7 (95% over 24bp)
GAATGATACCTGTCTCTTCTACACATCTGACGCAGAGCATCTCGTATGCCG	499	0.11473559079632019	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCC	482	0.11082676305376017	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGAGCATCTCGTATGCCGTCT	461	0.10599821113648016	Illumina PCR Primer Index 7 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05656303674528009	0.0
2	0.0	0.0	0.0	0.39387187782384064	0.0
3	0.0	0.0	0.0	0.5270019521145608	0.0
4	0.0	0.0	0.0	1.0418175589140817	0.0
5	0.0	0.0	0.0	2.431520786916004	0.0
6	0.0	0.0	0.0	2.9058685300278446	0.0
7	0.0	0.0	0.0	3.3880339286248056	0.0
8	0.0	0.0	0.0	3.9789567108824064	0.0
9	0.0	0.0	0.0	4.388463899676487	0.0
10	0.0	0.0	0.0	6.70823819936401	0.0
11	0.0	0.0	0.0	7.315716016766571	0.0
12	0.0	0.0	0.0	8.693002968409774	0.0
13	0.0	0.0	0.0	8.889364079712495	0.0
14	0.0	0.0	0.0	9.026173050702095	0.0
15	0.0	0.0	0.0	9.274728508920175	0.0
16	0.0	0.0	0.0	9.432921066972016	0.0
17	0.0	0.0	0.0	9.550415830292495	0.0
18	0.0	0.0	0.0	9.653884799948495	0.0
19	0.0	0.0	0.0	10.030971711583696	0.0
20	0.0	0.0	0.0	10.151225647428337	0.0
21	0.0	0.0	0.0	10.272399307447696	0.0
22	0.0	0.0	0.0	10.449906073168657	0.0
23	0.0	0.0	0.0	10.583496009546737	0.0
24	0.0	0.0	0.0	10.693862910513138	0.0
25	0.0	0.0	0.0	10.783076155460977	0.0
26	0.0	0.0	0.0	10.886085263029617	0.0
27	0.0	0.0	0.0	11.022664302975537	0.0
28	0.0	0.0	0.0	11.120384996539538	0.0
29	0.0	0.0	0.0	11.232131483768018	0.0
30	0.0	0.0	0.0	11.353765005874738	0.0
31	0.0	0.0	0.0	11.447346940652498	0.0
32	0.0	0.0	0.0	11.560702945186739	0.0
33	0.0	0.0	0.0	11.674058949720978	0.0
34	0.0	0.0	0.0	11.798221713308179	0.0
35	0.0	0.0	0.0	11.94261840873922	0.0
36	0.0	0.0	0.0	12.064711792933299	0.0
37	0.0	0.0	0.0	12.20795883314594	0.0
38	0.0	0.0	0.0	12.398341737312979	0.0
39	0.0	0.0	0.0	12.7591035448469	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCCTAA	20	7.028444E-4	45.000004	29
CTACGGC	40	6.7975634E-9	45.000004	6
GGCGATA	20	7.028444E-4	45.000004	8
TATACGG	20	7.028444E-4	45.000004	27
CATCGAC	25	3.8867933E-5	45.0	22
CGATGAA	125	0.0	44.999996	19
CGTTTTT	16525	0.0	44.564297	1
TGATACC	785	0.0	44.14013	4
GATACCT	785	0.0	44.14013	5
ATACCTG	785	0.0	43.853504	6
GAATGCT	160	0.0	43.593754	1
CCGATGA	145	0.0	43.44828	18
ATGATCC	85	0.0	42.35294	3
ACCACCG	315	0.0	42.142857	14
ATGATAC	2215	0.0	41.851017	3
CGACCAC	525	0.0	41.57143	12
GAATGAT	2405	0.0	41.44491	1
GGACTAA	220	0.0	40.909092	8
AATGATA	2255	0.0	40.909092	2
GTTTTTT	18185	0.0	40.855103	2
>>END_MODULE