##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933232.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	554571
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.374271283568742	31.0	28.0	31.0	25.0	34.0
2	28.629279208613504	31.0	28.0	33.0	16.0	34.0
3	28.66694435879265	31.0	28.0	34.0	16.0	34.0
4	33.010712785197924	35.0	32.0	37.0	28.0	37.0
5	34.08456446514513	35.0	33.0	37.0	30.0	37.0
6	33.579471339107165	35.0	33.0	37.0	28.0	37.0
7	33.255920342030144	35.0	32.0	36.0	28.0	37.0
8	33.40970227437064	35.0	33.0	37.0	28.0	37.0
9	34.33981041201217	37.0	33.0	39.0	27.0	39.0
10	33.972750107740936	35.0	32.0	39.0	27.0	39.0
11	34.150932883255706	37.0	32.0	39.0	27.0	39.0
12	34.23214160134591	37.0	32.0	39.0	26.0	39.0
13	34.415126286805474	37.0	33.0	39.0	27.0	39.0
14	35.18854393756615	37.0	33.0	40.0	27.0	41.0
15	35.54690021656379	38.0	33.0	40.0	27.0	41.0
16	35.38960565914915	37.0	33.0	40.0	27.0	41.0
17	34.85211992693451	37.0	32.0	40.0	27.0	41.0
18	34.6956025468335	37.0	33.0	39.0	27.0	40.0
19	34.10866056825907	37.0	32.0	38.0	25.0	40.0
20	33.78612296712233	35.0	32.0	38.0	26.0	40.0
21	34.141361520887315	35.0	33.0	38.0	27.0	40.0
22	34.87355631650411	36.0	33.0	39.0	29.0	40.0
23	35.29247292050973	36.0	34.0	39.0	30.0	40.0
24	34.774661855740746	36.0	33.0	39.0	27.0	40.0
25	33.66573802092068	35.0	32.0	39.0	24.0	40.0
26	33.874420047207664	35.0	32.0	38.0	25.0	40.0
27	34.53348985071344	35.0	33.0	39.0	27.0	40.0
28	34.3816715984067	35.0	33.0	39.0	25.0	40.0
29	34.589635952835614	36.0	33.0	39.0	25.0	40.0
30	33.851710601528026	35.0	33.0	39.0	23.0	40.0
31	33.82862609115875	35.0	33.0	39.0	23.0	40.0
32	33.53880927780212	35.0	33.0	39.0	21.0	40.0
33	33.39944930405665	36.0	33.0	39.0	18.0	40.0
34	33.107504719864544	36.0	33.0	40.0	15.0	41.0
35	32.74080866110922	36.0	32.0	40.0	13.0	41.0
36	32.17978221003262	36.0	31.0	40.0	10.0	41.0
37	32.219796924108906	36.0	31.0	40.0	10.0	41.0
38	32.008273061519624	36.0	31.0	40.0	10.0	41.0
39	31.65234568702655	35.0	30.0	39.0	10.0	40.0
40	31.411649725643787	35.0	30.0	39.0	10.0	40.0
41	30.912570257009474	35.0	27.0	39.0	8.0	40.0
42	31.186665007726692	35.0	29.0	39.0	8.0	40.0
43	31.14188625081369	35.0	29.0	39.0	8.0	40.0
44	31.256124103135576	35.0	29.0	39.0	8.0	40.0
45	31.357481729120348	35.0	30.0	39.0	8.0	40.0
46	31.181644911111473	35.0	29.0	39.0	8.0	40.0
47	31.110238364429442	35.0	29.0	39.0	8.0	40.0
48	30.963198580524406	35.0	29.0	39.0	8.0	40.0
49	31.120651819153906	35.0	29.0	39.0	8.0	40.0
50	31.093881576930638	35.0	29.0	39.0	8.0	40.0
51	29.810341687538656	34.0	26.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	18.0
9	38.0
10	46.0
11	47.0
12	60.0
13	84.0
14	111.0
15	189.0
16	296.0
17	600.0
18	985.0
19	1808.0
20	2856.0
21	4087.0
22	5907.0
23	8195.0
24	11283.0
25	16333.0
26	20892.0
27	22533.0
28	22122.0
29	22217.0
30	24559.0
31	29379.0
32	34959.0
33	42092.0
34	47697.0
35	56073.0
36	62116.0
37	63408.0
38	47117.0
39	6461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.1258324001796	2.703891837113733	38.027592499427485	32.14268326327918
2	30.40422236287148	15.686720005193203	37.09209461006796	16.816963021867355
3	13.547949676416545	15.968198841987771	53.77489987756302	16.708951604032666
4	12.446016831027947	3.3964992760169572	64.90963285133914	19.247851041615952
5	21.85112456295046	3.9192456872068684	54.26302493278589	19.966604817056787
6	16.031491008365027	13.27945384810962	57.5798229622537	13.109232181271649
7	60.6719428170604	1.5947462092320008	34.46808433906569	3.265226634641912
8	60.112771854280155	9.294030881528244	26.880237156288374	3.7129601079032257
9	56.56065679597383	2.9687812741740913	25.986393085826702	14.484168844025383
10	35.15600346934838	17.464490570188488	33.002627256023125	14.376878704440008
11	27.977301373494107	17.611811652610758	38.5745017319694	15.83638524192574
12	22.04983672063631	15.827910222496309	42.69137765948815	19.430875397379236
13	20.426059061869445	16.71922981908538	48.11575073344982	14.738960385595352
14	17.293547625101205	21.71299977820694	42.99792091544635	17.995531681245502
15	14.855446822859472	16.240120741978934	51.161889099862776	17.742543335298816
16	18.47139500623004	18.77036484057046	40.46190659086032	22.29633356233918
17	18.710318426315116	18.886310319147594	43.596221223251845	18.807150031285445
18	19.213410005211234	17.9738933337661	42.95067719011632	19.86201947090634
19	18.97124083300425	20.486826754374103	39.2510607298254	21.29087168279625
20	20.179021261479594	21.59416918663255	43.63733408346271	14.589475468425142
21	19.101431557005323	23.264108653355475	42.30982146560134	15.32463832403786
22	17.642646297768906	16.258693656898757	44.52432601055591	21.57433403477643
23	18.112018118509624	21.793602622567715	43.78681900063292	16.30756025828974
24	20.015651738010103	19.54916503026664	40.72751730617	19.70766592555327
25	18.086232421096668	25.876398152806402	37.85015805009638	18.187211376000548
26	17.282548131799174	17.666809119120906	41.87885771163656	23.171785037443357
27	19.71920637754228	18.573996837194876	40.352632936089336	21.354163849173506
28	14.566935523134097	20.30127792473822	42.7896518209571	22.342134731170578
29	18.950864722461144	18.408283159415113	39.97324057695047	22.667611541173265
30	17.664464964810637	20.168923365989205	45.208638749592026	16.95797291960813
31	19.706584008179295	17.262712979943053	43.267318341564916	19.76338467031273
32	22.279022884355655	19.44025201462031	40.53782112660056	17.742903974423474
33	19.552951741075532	17.7214459465064	37.17991023692187	25.545692075496195
34	19.757614444318218	19.46784090765655	41.02288074926385	19.751663898761386
35	21.37021229022073	21.797028694251953	37.96934928079543	18.863409734731892
36	22.87245456397828	21.98420039994879	34.66391138375429	20.47943365231864
37	19.05815486204652	22.52461812824688	40.048794473566055	18.368432536140546
38	21.75248976235685	21.960578537283773	33.581813690221814	22.705118010137564
39	22.376395448012968	20.68950594243118	36.79078062141728	20.14331798813858
40	23.843836046241147	20.786157227839176	34.953143961728976	20.4168627641907
41	21.709213067398043	23.656303701419656	34.073004178004254	20.56147905317804
42	21.50094397290879	21.49066575785607	37.82184787881083	19.18654239042431
43	23.84942595267333	19.989685721034817	33.0603295159682	23.100558810323655
44	22.400017310677985	21.04942378883858	33.51563641084731	23.034922489636134
45	20.111581745168788	20.524693862463057	33.20170005283363	26.162024339534522
46	23.707153817996254	22.976138312317087	31.584413898310586	21.732293971376073
47	17.13035842119404	22.21140304848252	39.57257050945686	21.08566802086658
48	20.005734162082042	24.402105411209746	32.1154550093676	23.47670541734061
49	20.232756491053443	21.343705314558463	36.64219730205871	21.781340892329386
50	20.28486884456634	20.709521413849625	34.9049986385873	24.10061110299673
51	19.107021463437505	21.39960437888025	32.81202947864205	26.681344679040198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77.0
1	779.5
2	1482.0
3	13828.0
4	26174.0
5	19287.5
6	12401.0
7	11764.0
8	11127.0
9	10764.5
10	10402.0
11	9765.0
12	9128.0
13	8566.5
14	8005.0
15	7410.0
16	6815.0
17	6313.0
18	5811.0
19	5410.0
20	5009.0
21	4766.5
22	4524.0
23	4483.5
24	4443.0
25	4564.0
26	5036.5
27	5388.0
28	6017.0
29	6646.0
30	7450.0
31	8254.0
32	9034.0
33	9814.0
34	10736.0
35	11658.0
36	12359.5
37	13061.0
38	13887.0
39	14713.0
40	15572.5
41	16432.0
42	17457.0
43	18482.0
44	20652.0
45	22822.0
46	51417.5
47	80013.0
48	57934.0
49	35855.0
50	34505.0
51	33155.0
52	30473.5
53	27792.0
54	27778.0
55	27764.0
56	26790.0
57	25816.0
58	24072.0
59	22328.0
60	19937.0
61	17546.0
62	15669.5
63	13793.0
64	11967.0
65	10141.0
66	8708.5
67	7276.0
68	6130.0
69	4984.0
70	4287.5
71	3591.0
72	3127.0
73	2663.0
74	2300.5
75	1615.5
76	1293.0
77	963.0
78	633.0
79	479.0
80	325.0
81	238.0
82	151.0
83	122.0
84	93.0
85	61.0
86	29.0
87	27.0
88	25.0
89	16.5
90	8.0
91	5.5
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	554571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.85842886509216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.77406575517126	36.44370527737693
2	7.544113180690781	7.070105816552062
3	4.172941657547085	5.866124694550496
4	2.885856732664011	5.409068496895355
5	2.1164460894746977	4.95866692652263
6	1.5611688579658523	4.389235192643404
7	1.069274782771008	3.5073175441998514
8	0.7548995023619709	2.8298723705377506
9	0.5593786789226338	2.3590445431450924
>10	1.4786132530507712	10.29919877151223
>50	0.050804677119688016	1.6285322311663564
>100	0.026965559348880567	2.3348460497247823
>500	0.0011724156238643725	0.355262849918908
>1k	0.002735636455683536	3.1354693383049193
>5k	3.9080520795479085E-4	1.7255885746318917
>10k+	0.0011724156238643725	7.687961322317319
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	16099	2.9029646339242405	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13865	2.500130731682688	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG	12018	2.16708050006221	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC	9423	1.6991512358201204	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	4249	0.7661778203331945	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC	2918	0.5261724828741495	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	2213	0.399047191432657	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTCTGGT	2110	0.38047427651283605	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCT	2071	0.3734418135820301	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTA	1966	0.3545082595375525	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTT	1595	0.28760970191373153	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGT	897	0.16174664740853742	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8031956232835832E-4	0.0	0.0	0.0618496098786269	0.0
2	1.8031956232835832E-4	0.0	0.0	0.5333852653672839	0.0
3	1.8031956232835832E-4	0.0	0.0	0.6565435264375526	0.0
4	1.8031956232835832E-4	0.0	0.0	1.5768945725614936	0.0
5	1.8031956232835832E-4	0.0	0.0	5.000441782927704	0.0
6	1.8031956232835832E-4	0.0	0.0	5.466026892859525	0.0
7	1.8031956232835832E-4	0.0	0.0	6.040525018437675	0.0
8	1.8031956232835832E-4	0.0	0.0	6.79894909759075	0.0
9	1.8031956232835832E-4	0.0	0.0	6.983596329414989	0.0
10	1.8031956232835832E-4	0.0	0.0	9.774943154257976	0.0
11	1.8031956232835832E-4	0.0	0.0	10.027390541517677	0.0
12	1.8031956232835832E-4	0.0	0.0	12.207995008754514	0.0
13	1.8031956232835832E-4	0.0	0.0	12.434115739914276	0.0
14	1.8031956232835832E-4	0.0	0.0	12.592796954763232	0.0
15	1.8031956232835832E-4	0.0	0.0	13.079118814362813	0.0
16	1.8031956232835832E-4	0.0	0.0	13.350139116542337	0.0
17	1.8031956232835832E-4	0.0	0.0	13.486280386100248	0.0
18	1.8031956232835832E-4	0.0	0.0	13.608537049358874	0.0
19	1.8031956232835832E-4	0.0	0.0	14.18123197931374	0.0
20	1.8031956232835832E-4	0.0	0.0	14.335585524666815	0.0
21	1.8031956232835832E-4	0.0	0.0	14.47443158765965	0.0
22	1.8031956232835832E-4	0.0	0.0	14.733009840038516	0.0
23	1.8031956232835832E-4	0.0	0.0	14.87744580946353	0.0
24	1.8031956232835832E-4	0.0	0.0	15.004571100905023	0.0
25	1.8031956232835832E-4	0.0	0.0	15.11366443611368	0.0
26	1.8031956232835832E-4	0.0	0.0	15.276132361771532	0.0
27	1.8031956232835832E-4	0.0	0.0	15.457714161036188	0.0
28	1.8031956232835832E-4	0.0	0.0	15.576725072172906	0.0
29	1.8031956232835832E-4	0.0	0.0	15.725849350218457	0.0
30	1.8031956232835832E-4	0.0	0.0	15.903644438674219	0.0
31	1.8031956232835832E-4	0.0	0.0	16.04609689291362	0.0
32	1.8031956232835832E-4	0.0	0.0	16.220285590122817	0.0
33	1.8031956232835832E-4	0.0	0.0	16.378425846284784	0.0
34	1.8031956232835832E-4	0.0	0.0	16.608152968691115	0.0
35	1.8031956232835832E-4	0.0	0.0	16.8140779088701	0.0
36	1.8031956232835832E-4	0.0	0.0	16.987365008267652	0.0
37	1.8031956232835832E-4	0.0	0.0	17.171831920529563	0.0
38	1.8031956232835832E-4	0.0	0.0	17.428606977285146	0.0
39	1.8031956232835832E-4	0.0	0.0	17.92124002156622	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	20	7.0301257E-4	45.0	24
TCTTACG	25	3.8881866E-5	44.999996	1
CGTTTTT	9735	0.0	43.936825	1
GAATCTG	2010	0.0	41.97761	1
ACGGCTG	1175	0.0	40.97872	8
CGACCAC	1160	0.0	40.926723	12
TACGAAT	55	6.002665E-11	40.909092	12
CTCGTCC	105	0.0	40.714287	37
CACCGGT	100	0.0	40.499996	16
AATCTGT	2085	0.0	40.467625	2
TACGGCT	1195	0.0	40.29289	7
GATACCT	1325	0.0	40.245285	5
TGATACC	1320	0.0	40.227272	4
GCGACCA	1200	0.0	39.937504	11
TTACGGG	120	0.0	39.375004	3
ATACCTG	1360	0.0	39.375004	6
AGGGTAC	40	3.4539153E-7	39.375	6
TATACGG	40	3.4539153E-7	39.375	27
GAATGAT	4675	0.0	39.272724	1
ATGATAC	4515	0.0	39.269104	3
>>END_MODULE