##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933231.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262144
Sequences flagged as poor quality	0
Sequence length	51
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.65245819091797	31.0	28.0	31.0	16.0	33.0
2	28.87145233154297	31.0	30.0	33.0	16.0	34.0
3	29.058490753173828	31.0	30.0	33.0	16.0	34.0
4	32.2707633972168	35.0	32.0	37.0	19.0	37.0
5	33.7367057800293	35.0	33.0	37.0	28.0	37.0
6	32.83861541748047	35.0	33.0	37.0	18.0	37.0
7	32.19243621826172	35.0	32.0	35.0	26.0	37.0
8	32.97736358642578	35.0	32.0	35.0	28.0	37.0
9	32.5994873046875	35.0	31.0	37.0	25.0	39.0
10	30.59168243408203	34.0	27.0	37.0	15.0	39.0
11	31.441810607910156	35.0	27.0	39.0	15.0	39.0
12	32.34111022949219	35.0	30.0	39.0	21.0	39.0
13	32.57926559448242	35.0	30.0	38.0	21.0	39.0
14	32.948272705078125	36.0	31.0	39.0	18.0	41.0
15	33.441551208496094	36.0	31.0	39.0	22.0	41.0
16	33.525306701660156	36.0	31.0	39.0	23.0	41.0
17	31.44550323486328	34.0	27.0	38.0	17.0	40.0
18	32.3422737121582	34.0	29.0	38.0	19.0	39.0
19	32.292972564697266	35.0	30.0	37.0	18.0	39.0
20	32.277896881103516	34.0	30.0	37.0	23.0	39.0
21	32.79261779785156	35.0	31.0	37.0	23.0	39.0
22	33.62342071533203	35.0	31.0	38.0	26.0	39.0
23	34.52060317993164	35.0	33.0	39.0	28.0	40.0
24	32.632598876953125	35.0	31.0	38.0	23.0	39.0
25	31.75323486328125	35.0	30.0	37.0	18.0	39.0
26	31.645294189453125	34.0	28.0	37.0	21.0	39.0
27	33.03816223144531	35.0	31.0	38.0	24.0	40.0
28	32.998470306396484	35.0	31.0	38.0	23.0	40.0
29	33.479583740234375	35.0	32.0	38.0	24.0	40.0
30	32.41455841064453	35.0	30.0	38.0	21.0	40.0
31	32.530189514160156	35.0	30.0	38.0	22.0	40.0
32	32.56645584106445	35.0	31.0	38.0	21.0	40.0
33	32.86322784423828	35.0	31.0	38.0	21.0	40.0
34	32.3804931640625	35.0	31.0	38.0	18.0	40.0
35	31.976821899414062	35.0	31.0	38.0	15.0	40.0
36	31.312053680419922	35.0	30.0	38.0	12.0	40.0
37	31.514076232910156	35.0	30.0	38.0	10.0	40.0
38	31.37884521484375	35.0	30.0	38.0	10.0	40.0
39	30.896820068359375	35.0	29.0	38.0	10.0	40.0
40	30.57569122314453	35.0	27.0	38.0	10.0	40.0
41	30.10213851928711	34.0	25.0	38.0	10.0	40.0
42	30.663841247558594	35.0	27.0	38.0	10.0	40.0
43	30.727928161621094	35.0	28.0	38.0	10.0	40.0
44	30.646446228027344	35.0	28.0	38.0	10.0	40.0
45	30.93633270263672	35.0	29.0	38.0	9.0	40.0
46	30.71524429321289	35.0	29.0	38.0	8.0	40.0
47	30.721736907958984	35.0	29.0	38.0	8.0	40.0
48	30.529388427734375	35.0	28.0	38.0	8.0	40.0
49	30.427959442138672	35.0	28.0	38.0	8.0	40.0
50	30.555267333984375	35.0	28.0	38.0	8.0	40.0
51	28.807697296142578	33.0	24.0	37.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	9.0
11	15.0
12	15.0
13	24.0
14	48.0
15	86.0
16	209.0
17	390.0
18	630.0
19	1108.0
20	1661.0
21	2338.0
22	3136.0
23	4408.0
24	6060.0
25	8601.0
26	11128.0
27	12662.0
28	13115.0
29	14176.0
30	16306.0
31	19152.0
32	22582.0
33	25696.0
34	29079.0
35	30927.0
36	23444.0
37	12516.0
38	2564.0
39	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.998641967773438	2.74810791015625	40.12413024902344	33.129119873046875
2	32.3577880859375	12.207794189453125	39.13154602050781	16.302871704101562
3	13.981246948242188	12.247085571289062	57.44056701660156	16.331100463867188
4	12.926864624023438	3.32183837890625	64.56527709960938	19.186019897460938
5	19.98748779296875	3.8089752197265625	56.80656433105469	19.39697265625
6	16.437911987304688	10.379791259765625	59.12666320800781	14.055633544921875
7	59.81712341308594	1.6448974609375	34.77821350097656	3.759765625
8	61.084747314453125	7.2826385498046875	27.206039428710938	4.42657470703125
9	56.43653869628906	2.7679443359375	27.334213256835938	13.4613037109375
10	33.8623046875	17.209243774414062	33.95576477050781	14.972686767578125
11	26.282119750976562	17.539596557617188	40.54450988769531	15.633773803710938
12	21.652603149414062	15.4937744140625	43.92242431640625	18.931198120117188
13	19.480133056640625	16.74957275390625	48.60801696777344	15.162277221679688
14	16.802597045898438	19.017791748046875	45.20301818847656	18.976593017578125
15	14.48211669921875	16.1041259765625	51.25083923339844	18.162918090820312
16	17.511749267578125	16.71295166015625	42.487335205078125	23.2879638671875
17	17.798233032226562	17.891311645507812	44.948577880859375	19.36187744140625
18	18.032073974609375	16.657638549804688	44.32487487792969	20.98541259765625
19	18.17169189453125	19.999313354492188	40.850067138671875	20.978927612304688
20	18.978500366210938	21.045684814453125	44.77691650390625	15.198898315429688
21	18.808746337890625	22.055435180664062	42.53807067871094	16.597747802734375
22	17.1661376953125	16.960525512695312	44.683074951171875	21.190261840820312
23	17.546463012695312	21.350860595703125	43.32122802734375	17.781448364257812
24	18.550491333007812	19.048690795898438	41.85829162597656	20.542526245117188
25	17.426681518554688	22.971725463867188	39.369964599609375	20.23162841796875
26	16.961288452148438	18.43414306640625	42.36640930175781	22.2381591796875
27	17.885208129882812	18.474960327148438	41.814422607421875	21.825408935546875
28	14.617156982421875	19.377899169921875	43.82896423339844	22.175979614257812
29	18.434524536132812	17.641067504882812	41.21818542480469	22.706222534179688
30	17.502212524414062	19.04296875	45.2239990234375	18.230819702148438
31	18.814468383789062	17.447280883789062	43.72978210449219	20.008468627929688
32	20.886993408203125	18.969345092773438	41.15409851074219	18.98956298828125
33	18.51043701171875	17.333602905273438	39.6148681640625	24.541091918945312
34	19.017410278320312	18.799972534179688	41.529083251953125	20.653533935546875
35	19.633102416992188	20.691299438476562	40.097808837890625	19.577789306640625
36	21.2188720703125	20.2484130859375	37.9791259765625	20.5535888671875
37	18.67828369140625	21.068572998046875	41.88385009765625	18.369293212890625
38	20.943450927734375	20.04547119140625	37.282562255859375	21.728515625
39	21.112442016601562	19.524383544921875	39.37492370605469	19.988250732421875
40	23.101806640625	19.597625732421875	37.155914306640625	20.1446533203125
41	21.4447021484375	21.445465087890625	37.044525146484375	20.0653076171875
42	21.457672119140625	20.0775146484375	39.37721252441406	19.087600708007812
43	22.4273681640625	18.925857543945312	35.840606689453125	22.806167602539062
44	21.210098266601562	20.03326416015625	36.38801574707031	22.368621826171875
45	19.573593139648438	19.718551635742188	35.7452392578125	24.962615966796875
46	22.000885009765625	21.7987060546875	34.378814697265625	21.82159423828125
47	17.014312744140625	20.9747314453125	40.659332275390625	21.35162353515625
48	19.461822509765625	22.501754760742188	34.987640380859375	23.048782348632812
49	19.469070434570312	20.370864868164062	37.64915466308594	22.510910034179688
50	19.852447509765625	19.410324096679688	36.516571044921875	24.220657348632812
51	18.956756591796875	19.633865356445312	35.15892028808594	26.250457763671875
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	60.0
1	293.0
2	526.0
3	9187.0
4	17848.0
5	11848.0
6	5848.0
7	5626.5
8	5405.0
9	5374.0
10	5343.0
11	4893.0
12	4443.0
13	3995.0
14	3547.0
15	3206.0
16	2865.0
17	2634.0
18	2403.0
19	2242.0
20	2081.0
21	1934.5
22	1788.0
23	1811.0
24	1834.0
25	1849.0
26	2041.5
27	2219.0
28	2455.5
29	2692.0
30	3059.0
31	3426.0
32	3780.0
33	4134.0
34	4600.0
35	5066.0
36	5354.0
37	5642.0
38	6064.5
39	6487.0
40	7067.0
41	7647.0
42	8302.0
43	8957.0
44	10006.5
45	11056.0
46	20530.0
47	30004.0
48	25818.0
49	21632.0
50	20454.5
51	19277.0
52	16852.0
53	14427.0
54	13437.5
55	12448.0
56	11738.0
57	11028.0
58	10240.0
59	9452.0
60	8506.5
61	7561.0
62	6886.0
63	6211.0
64	5517.0
65	4823.0
66	4216.5
67	3610.0
68	3097.5
69	2585.0
70	2293.0
71	2001.0
72	1694.0
73	1387.0
74	1218.5
75	905.0
76	760.0
77	561.0
78	362.0
79	269.0
80	176.0
81	137.5
82	99.0
83	71.5
84	44.0
85	29.0
86	14.0
87	10.5
88	7.0
89	5.5
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	262144.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.683237557846006
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.554963883397	41.63401549942567
2	10.340986041302555	11.102752204752292
3	4.968661584398051	8.002015205393521
4	2.802892987180722	6.018734803201733
5	1.6317530408324759	4.37988930633737
6	0.935207312099778	3.0122973780772204
7	0.5049235373339738	1.897415114227335
8	0.3283333266810111	1.4100796779499662
9	0.19438376621489742	0.9391634909192824
>10	0.6651852897552003	5.939170224684161
>50	0.03383498859214559	1.210012742075433
>100	0.03167530846924268	3.0140176861853405
>500	0.0035994668715048504	1.4067219358526273
>1k	0.0028795734972038804	4.998886879190586
>5k	0.0	0.0
>10k+	7.198933743009701E-4	5.0348278517274805
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13028	4.96978759765625	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCG	4236	1.61590576171875	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	3878	1.479339599609375	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGC	3277	1.2500762939453125	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCT	1544	0.5889892578125	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	918	0.350189208984375	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTTCTGGT	903	0.3444671630859375	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTC	738	0.281524658203125	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTA	562	0.214385986328125	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCCTTTCTGGTCGTATGCCG	519	0.1979827880859375	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTT	441	0.1682281494140625	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCT	434	0.165557861328125	No Hit
GAATCTGTCTCTTATCCCCATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	370	0.141143798828125	No Hit
GAATCTGTCTCTTATCCACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	348	0.13275146484375	No Hit
GAATCTTTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTC	318	0.121307373046875	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	307	0.1171112060546875	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGT	289	0.1102447509765625	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCC	263	0.1003265380859375	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.144195556640625	0.0
2	0.0	0.0	0.0	0.6855010986328125	0.0
3	0.0	0.0	0.0	0.9063720703125	0.0
4	0.0	0.0	0.0	1.9321441650390625	0.0
5	0.0	0.0	0.0	3.995513916015625	0.0
6	0.0	0.0	0.0	4.6875	0.0
7	0.0	0.0	0.0	5.3436279296875	0.0
8	0.0	0.0	0.0	6.2503814697265625	0.0
9	0.0	0.0	0.0	6.7596435546875	0.0
10	0.0	0.0	0.0	9.412765502929688	0.0
11	0.0	0.0	0.0	10.2630615234375	0.0
12	0.0	0.0	0.0	12.266921997070312	0.0
13	0.0	0.0	0.0	12.604904174804688	0.0
14	0.0	0.0	0.0	12.803268432617188	0.0
15	0.0	0.0	0.0	13.232803344726562	0.0
16	0.0	0.0	0.0	13.536453247070312	0.0
17	0.0	0.0	0.0	13.797760009765625	0.0
18	3.814697265625E-4	0.0	0.0	14.059066772460938	0.0
19	3.814697265625E-4	0.0	0.0	14.714431762695312	0.0
20	3.814697265625E-4	0.0	0.0	15.010833740234375	0.0
21	3.814697265625E-4	0.0	0.0	15.318679809570312	0.0
22	3.814697265625E-4	0.0	0.0	15.724563598632812	0.0
23	3.814697265625E-4	0.0	0.0	16.026687622070312	0.0
24	3.814697265625E-4	0.0	0.0	16.271209716796875	0.0
25	3.814697265625E-4	0.0	0.0	16.480255126953125	0.0
26	3.814697265625E-4	0.0	0.0	16.686248779296875	0.0
27	3.814697265625E-4	0.0	0.0	16.920089721679688	0.0
28	3.814697265625E-4	0.0	0.0	17.121124267578125	0.0
29	3.814697265625E-4	0.0	0.0	17.370986938476562	0.0
30	3.814697265625E-4	0.0	0.0	17.63916015625	0.0
31	3.814697265625E-4	0.0	0.0	17.850112915039062	0.0
32	3.814697265625E-4	0.0	0.0	18.085098266601562	0.0
33	3.814697265625E-4	0.0	0.0	18.310928344726562	0.0
34	3.814697265625E-4	0.0	0.0	18.570709228515625	0.0
35	3.814697265625E-4	0.0	0.0	18.852615356445312	0.0
36	3.814697265625E-4	0.0	0.0	19.08111572265625	0.0
37	3.814697265625E-4	0.0	0.0	19.310760498046875	0.0
38	3.814697265625E-4	0.0	0.0	19.613265991210938	0.0
39	3.814697265625E-4	0.0	0.0	20.223236083984375	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAATAG	30	2.1590677E-6	45.000004	26
AACGAAT	30	2.1590677E-6	45.000004	24
CGTAACC	30	2.1590677E-6	45.000004	35
CACCGGT	75	0.0	45.000004	16
CGTTTTT	5145	0.0	44.0379	1
GATACCT	535	0.0	42.056072	5
GAATGAT	1790	0.0	41.606148	1
ACACGCG	125	0.0	41.4	36
GCGAGAC	120	0.0	41.250004	21
TCTTGCG	60	3.6379788E-12	41.250004	1
TCAAGCG	120	0.0	41.250004	17
TTACACG	120	0.0	41.250004	34
TACACGC	120	0.0	41.250004	35
AATGATA	1740	0.0	40.99138	2
TGATACC	550	0.0	40.909092	4
ACACGAC	110	0.0	40.909092	26
CACGACC	110	0.0	40.909092	27
ACGTGAG	110	0.0	40.909092	44
ATGATAC	1770	0.0	40.805088	3
TACGGCT	555	0.0	40.54054	7
>>END_MODULE