##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933230.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1680623
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.222173563017996	33.0	31.0	34.0	30.0	34.0
2	32.41344370510222	34.0	31.0	34.0	30.0	34.0
3	32.47874984455169	34.0	31.0	34.0	30.0	34.0
4	35.956767817648576	37.0	35.0	37.0	35.0	37.0
5	35.18792257395025	37.0	35.0	37.0	35.0	37.0
6	34.87455306752317	37.0	35.0	37.0	32.0	37.0
7	35.64669827796002	37.0	35.0	37.0	35.0	37.0
8	35.92638325192503	37.0	35.0	37.0	35.0	37.0
9	37.80331817427228	39.0	38.0	39.0	35.0	39.0
10	37.27837117545101	39.0	37.0	39.0	34.0	39.0
11	37.21087656184641	39.0	37.0	39.0	34.0	39.0
12	37.045824673350296	39.0	37.0	39.0	33.0	39.0
13	37.00913470778396	39.0	37.0	39.0	33.0	39.0
14	38.161424662163974	40.0	37.0	41.0	33.0	41.0
15	38.25520417131028	40.0	37.0	41.0	33.0	41.0
16	38.24275997650871	40.0	37.0	41.0	33.0	41.0
17	38.14793323666283	40.0	37.0	41.0	33.0	41.0
18	38.13484463797056	40.0	37.0	41.0	33.0	41.0
19	38.10042525896647	40.0	37.0	41.0	34.0	41.0
20	38.019210733162645	40.0	37.0	41.0	33.0	41.0
21	37.886189228637235	40.0	36.0	41.0	33.0	41.0
22	37.93642833639668	40.0	36.0	41.0	33.0	41.0
23	37.84343663034482	40.0	36.0	41.0	33.0	41.0
24	37.79009272156813	40.0	36.0	41.0	33.0	41.0
25	37.669955129734625	40.0	36.0	41.0	33.0	41.0
26	37.621515949740065	40.0	36.0	41.0	33.0	41.0
27	37.56936802602368	40.0	36.0	41.0	33.0	41.0
28	37.46484488192771	39.0	35.0	41.0	33.0	41.0
29	37.394107423259115	39.0	35.0	41.0	33.0	41.0
30	37.28269040706928	39.0	35.0	41.0	32.0	41.0
31	37.2155147228141	39.0	35.0	41.0	32.0	41.0
32	37.16717134062785	39.0	35.0	41.0	32.0	41.0
33	37.04156494347632	39.0	35.0	41.0	31.0	41.0
34	36.90595511307414	39.0	35.0	41.0	31.0	41.0
35	36.76586718139642	39.0	35.0	41.0	31.0	41.0
36	36.61348618934764	39.0	35.0	41.0	31.0	41.0
37	36.585016984772906	39.0	35.0	41.0	31.0	41.0
38	36.468595871887985	39.0	35.0	41.0	30.0	41.0
39	36.435671176700545	39.0	35.0	41.0	30.0	41.0
40	36.32468376310452	39.0	35.0	40.0	30.0	41.0
41	36.17607101652185	38.0	35.0	40.0	30.0	41.0
42	36.071858471531094	38.0	35.0	40.0	30.0	41.0
43	35.90740457556513	38.0	35.0	40.0	29.0	41.0
44	35.81248798808537	38.0	35.0	40.0	29.0	41.0
45	35.76276595048384	38.0	35.0	40.0	29.0	41.0
46	35.51298774323569	38.0	34.0	40.0	28.0	41.0
47	35.412693388106675	37.0	34.0	40.0	28.0	41.0
48	35.32342827629992	37.0	34.0	40.0	28.0	41.0
49	35.37297240368601	37.0	34.0	40.0	28.0	41.0
50	35.19294511618608	37.0	34.0	40.0	28.0	41.0
51	34.12564209819811	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	14.0
9	24.0
10	33.0
11	37.0
12	21.0
13	26.0
14	31.0
15	62.0
16	108.0
17	201.0
18	391.0
19	698.0
20	1264.0
21	2078.0
22	3165.0
23	4758.0
24	7020.0
25	10161.0
26	13992.0
27	17066.0
28	20107.0
29	23903.0
30	29309.0
31	36910.0
32	48082.0
33	66821.0
34	110915.0
35	136817.0
36	154720.0
37	224966.0
38	352160.0
39	414638.0
40	125.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.395060641202697	4.5284992529556005	53.43792153266973	17.63851857317197
2	20.94842210299395	4.377900338148413	51.96584837884522	22.70782918001241
3	21.42211548931557	4.577052676299206	50.936706209542535	23.064125624842692
4	18.378482265207605	5.043486849817002	50.38209045098158	26.195940433993826
5	17.91924780274934	7.77640196522361	49.52639586629482	24.77795436573223
6	22.28489078157326	7.279086386417418	52.12697910239239	18.309043729616935
7	84.80319500566159	2.0626279659388214	8.79614285892791	4.338034169471678
8	86.97661521947516	2.488124939382598	6.865489761832368	3.6697700793098753
9	81.8280482892356	4.744669089974372	8.655897247627815	4.7713853731622144
10	39.25002811457418	27.737749632130466	15.998650500439421	17.013571752855935
11	31.03468178169643	23.81420461340824	23.94141934270803	21.209694262187295
12	24.777835362243643	21.923715193710905	31.009274536883048	22.289174907162405
13	23.63974549913931	22.88234779602564	32.52710453206936	20.950802172765695
14	22.0686019410659	23.71233762717754	32.4519538290265	21.76710660273006
15	20.478239319585654	21.39724376020083	34.19547394031856	23.929042979894955
16	23.609161602572378	21.203446579036463	31.769825832444276	23.41756598594688
17	22.69521481022216	21.567597254113505	30.190352030169766	25.546835905494568
18	25.205295893249115	20.331865028623312	29.383686882780967	25.079152195346605
19	24.783131017485776	23.090663402797652	27.6850905884306	24.44111499128597
20	28.395838924018058	22.96713778164407	26.868607653233354	21.768415641104518
21	27.127321237422073	24.313602753264714	27.029678875036222	21.52939713427699
22	26.568540356760558	21.588422864616277	26.71902026807916	25.124016510544006
23	24.860661790300384	23.911727972305506	26.62149690918189	24.606113328212217
24	24.725830837731007	22.67355617529928	28.563574341181813	24.037038645787902
25	24.90891770492252	24.090114201697823	25.907832988124046	25.09313510525561
26	24.11617596569843	24.28998056078014	26.39872237854653	25.195121094974898
27	23.543947690826556	22.8229055534763	28.017229325077665	25.61591743061948
28	21.331018318802016	24.747072960443838	28.688111491988387	25.23379722876576
29	22.95975956535166	22.698368402669725	28.018835872173593	26.323036159805024
30	24.467176755286584	21.69326493806166	30.195290674946136	23.644267631705624
31	25.898134203804187	23.714360686483523	26.59835073065167	23.789154379060623
32	24.716905576086965	23.078584548706047	27.233888861451973	24.970621013755018
33	24.181211372211376	22.60566468506024	26.952148102221617	26.260975840506763
34	23.23061150537628	22.948930247890218	29.274084669792096	24.54637357694141
35	23.57857770600545	22.959819067095953	28.115942718860804	25.345660508037792
36	23.769340298210842	25.114377227968436	27.273041009197186	23.843241464623535
37	23.306000215396313	23.865495116989354	28.120048339217064	24.708456328397265
38	22.023797127612795	24.9894830666961	28.196746087611558	24.789973718079544
39	23.445472304020594	23.123448863903445	27.485581239814046	25.94549759226192
40	25.339115316165493	22.78494344061696	27.892454167293913	23.98348707592363
41	22.837780989549707	23.256435262399716	27.440895429849526	26.464888318201048
42	23.108216417364275	22.935304348446977	27.97926721221833	25.977212021970423
43	23.25613775367825	22.078955244572995	28.974195878552177	25.690711123196575
44	24.16377736113334	22.278405091445254	28.307002819787662	25.250814727633742
45	24.52001430421933	21.450378817855047	26.915554529481035	27.114052348444595
46	23.863650562916252	22.115429813824992	28.379594947825897	25.64132467543286
47	22.234968818110904	23.78427523602854	30.108180121300254	23.872575824560297
48	22.101863416126044	23.140585366260012	29.65275377047678	25.104797447137162
49	23.365739966667125	22.326065988624457	29.5590980249586	24.74909601974982
50	22.94458662055678	22.14851278365225	28.671748512307637	26.235152083483328
51	22.408594907959728	21.400278349159805	27.713353916970075	28.477772825910392
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	254.0
1	1042.5
2	1831.0
3	5810.5
4	9790.0
5	6640.0
6	3490.0
7	3422.5
8	3355.0
9	3422.5
10	3490.0
11	3672.0
12	3854.0
13	3939.0
14	4024.0
15	3816.0
16	3608.0
17	3639.5
18	3671.0
19	3821.0
20	3971.0
21	3982.5
22	3994.0
23	4494.5
24	4995.0
25	6024.0
26	8748.5
27	10444.0
28	12242.5
29	14041.0
30	16269.5
31	18498.0
32	21895.0
33	25292.0
34	30768.5
35	36245.0
36	39599.0
37	42953.0
38	45971.0
39	48989.0
40	54360.0
41	59731.0
42	67701.0
43	75671.0
44	84303.5
45	92936.0
46	104751.0
47	116566.0
48	133844.0
49	151122.0
50	152449.5
51	153777.0
52	142834.0
53	131891.0
54	121721.0
55	111551.0
56	105918.5
57	100286.0
58	97529.5
59	94773.0
60	89210.5
61	83648.0
62	77543.5
63	71439.0
64	64393.0
65	57347.0
66	49182.5
67	41018.0
68	34823.0
69	28628.0
70	23830.0
71	19032.0
72	16771.5
73	14511.0
74	12585.5
75	8508.0
76	6356.0
77	4721.5
78	3087.0
79	2299.0
80	1511.0
81	1110.5
82	710.0
83	517.0
84	324.0
85	229.0
86	134.0
87	94.0
88	54.0
89	35.0
90	16.0
91	8.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1680623.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.634946217006654
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.47469901397575	24.509079364859012
2	9.183264387966311	5.810241500197336
3	2.9709921371952026	2.8196152941395964
4	1.480299023458679	1.8731671996881114
5	0.8309875281429346	1.3144122879902522
6	0.5794984992542511	1.0999442314046586
7	0.4258281680606501	0.9429735835999595
8	0.36466201891327527	0.9228850684565221
9	0.30241196469724546	0.8610107614719009
>10	6.016178991247557	48.13696976057998
>50	0.3012838533453974	5.882939674484811
>100	0.06430286758556995	3.590421940034174
>500	0.0035413125661897297	0.8039571505676916
>1k	0.001863848719047226	1.114926715077703
>5k	1.8638487190472263E-4	0.31745546744821795
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5384	0.3203573912769253	No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	3126	0.18600245266189977	No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT	2873	0.1709485113556104	No Hit
TCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	2652	0.15779862586671728	No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC	2465	0.14667179968380772	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.3800697717453587E-4	0.0	0.0	0.18558594045184434	0.0
2	2.3800697717453587E-4	0.0	0.0	0.7338350123733878	0.0
3	2.3800697717453587E-4	0.0	0.0	1.1244639636610947	0.0
4	2.3800697717453587E-4	0.0	0.0	1.5853644749595834	0.0
5	2.3800697717453587E-4	0.0	0.0	2.3422266623746073	0.0
6	2.3800697717453587E-4	0.0	0.0	3.018701993248932	0.0
7	2.3800697717453587E-4	0.0	0.0	3.6087807914089	0.0
8	2.3800697717453587E-4	0.0	0.0	4.5053530744253765	0.0
9	2.3800697717453587E-4	0.0	0.0	5.012962454994368	0.0
10	2.3800697717453587E-4	0.0	0.0	5.816236002958426	0.0
11	2.3800697717453587E-4	0.0	0.0	7.139733301281727	0.0
12	2.3800697717453587E-4	0.0	0.0	8.278537185317587	0.0
13	2.3800697717453587E-4	0.0	0.0	8.661371408102829	0.0
14	2.3800697717453587E-4	0.0	0.0	8.794476810087687	0.0
15	2.3800697717453587E-4	0.0	0.0	8.99214160463114	0.0
16	2.3800697717453587E-4	0.0	0.0	9.466430008395696	0.0
17	2.3800697717453587E-4	0.0	0.0	10.166587033498887	0.0
18	2.3800697717453587E-4	0.0	0.0	10.878584905716512	0.0
19	2.3800697717453587E-4	0.0	0.0	11.351326264129433	0.0
20	2.3800697717453587E-4	0.0	0.0	11.851854937127483	0.0
21	2.3800697717453587E-4	0.0	0.0	12.55344000409372	0.0
22	2.9750872146816986E-4	0.0	0.0	13.287096511234227	0.0
23	2.9750872146816986E-4	0.0	0.0	13.98874107994476	0.0
24	2.9750872146816986E-4	0.0	0.0	14.56085035132805	0.0
25	3.570104657618038E-4	0.0	0.0	15.061855038280447	0.0
26	3.570104657618038E-4	0.0	0.0	15.505500043733782	0.0
27	3.570104657618038E-4	0.0	0.0	15.95378618524202	0.0
28	3.570104657618038E-4	0.0	0.0	16.42420697562749	0.0
29	3.570104657618038E-4	0.0	0.0	16.941991154470692	0.0
30	3.570104657618038E-4	0.0	0.0	17.548908946265758	0.0
31	3.570104657618038E-4	0.0	0.0	18.043130434368685	0.0
32	3.570104657618038E-4	0.0	0.0	18.53330580385964	0.0
33	3.570104657618038E-4	0.0	0.0	19.02824131289409	0.0
34	3.570104657618038E-4	0.0	0.0	19.538944784166347	0.0
35	3.570104657618038E-4	0.0	0.0	20.101712281695537	0.0
36	3.570104657618038E-4	0.0	0.0	20.59688579770716	0.0
37	3.570104657618038E-4	0.0	0.0	21.10937432130823	0.0
38	3.570104657618038E-4	0.0	0.0	21.656076347878138	0.0
39	3.570104657618038E-4	0.0	0.0	22.223901493672287	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAT	20	7.034219E-4	45.0	35
CGCGTAG	20	7.034219E-4	45.0	29
TATACGG	20	7.034219E-4	45.0	2
TACGGGA	370	0.0	40.135136	4
CCGTTAG	45	1.9292202E-8	40.0	1
GTTACCG	155	0.0	39.19355	38
CATCGAA	35	6.250015E-6	38.571426	12
ACCGGTA	135	0.0	38.333336	41
CGTTTTT	3125	0.0	38.016	1
TTTGGGC	4510	0.0	37.865856	4
ACACGAC	1585	0.0	37.76025	26
CGACCAA	1555	0.0	37.62058	29
ATTACGG	30	1.1401462E-4	37.500004	2
CACGACC	1590	0.0	37.5	27
TTTGGGA	7535	0.0	37.445255	4
TCAAGCG	1650	0.0	37.363636	17
TGGGCGA	2940	0.0	37.27041	6
ACGACCA	1580	0.0	37.025314	28
CGTTCAT	305	0.0	36.885246	17
GCGAGAC	1680	0.0	36.69643	21
>>END_MODULE