FastQCFastQC Report
Sat 14 Jan 2017
SRR2933229.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933229.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1892438
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93360.4933318819427638No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT28590.15107496256152117No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC28020.14806297485043105No Hit
TCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC25810.1363849172337482No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC20130.10637072390218333No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGC207.0344453E-445.010
CGAACGA600.044.99999622
GCGATCG650.044.9999969
CGTTTTT51750.040.3913041
GTCGTTA356.250433E-638.57142622
TTTGGGA99400.038.028174
ACGGGTA301.14020084E-437.4999965
TACGTCG301.14020084E-437.49999624
TTTGGGC55650.037.4393544
CGTTTGG13250.037.358492
AGACACG2300.037.17391624
TACGAAT3100.037.0161312
TTGGGAC39400.036.947975
TTGGGAT50950.036.8302275
TGGGCGA38550.036.5369646
TTACGGG4100.036.2195133
ACACGAC2300.036.19565626
GGGCGAT60450.036.178667
CCGCTAA250.002107452136.026
ATACGTA250.002107452136.037
Produced by FastQC (version 0.11.5)