##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933229.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1892438
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.234760663229125	33.0	31.0	34.0	30.0	34.0
2	32.42217922066668	34.0	31.0	34.0	30.0	34.0
3	32.48299865041814	34.0	31.0	34.0	30.0	34.0
4	35.96477189741487	37.0	35.0	37.0	35.0	37.0
5	35.16702845747126	37.0	35.0	37.0	35.0	37.0
6	34.83956673877823	37.0	35.0	37.0	32.0	37.0
7	35.63221674897672	37.0	35.0	37.0	33.0	37.0
8	35.91716241166157	37.0	35.0	37.0	35.0	37.0
9	37.77502301264295	39.0	38.0	39.0	35.0	39.0
10	37.295064884556325	39.0	37.0	39.0	34.0	39.0
11	37.24507381483568	39.0	37.0	39.0	34.0	39.0
12	37.11580987065362	39.0	37.0	39.0	34.0	39.0
13	37.10843472811263	39.0	37.0	39.0	33.0	39.0
14	38.30405487524558	40.0	38.0	41.0	33.0	41.0
15	38.37909934169574	40.0	38.0	41.0	34.0	41.0
16	38.36015710950636	40.0	38.0	41.0	34.0	41.0
17	38.302040542411426	40.0	38.0	41.0	34.0	41.0
18	38.23867043464568	40.0	37.0	41.0	34.0	41.0
19	38.21014638260276	40.0	37.0	41.0	34.0	41.0
20	38.107159124896036	40.0	37.0	41.0	34.0	41.0
21	37.99792014322266	40.0	37.0	41.0	33.0	41.0
22	38.0212794289694	40.0	37.0	41.0	33.0	41.0
23	37.915470414354395	40.0	36.0	41.0	33.0	41.0
24	37.84605783650508	40.0	36.0	41.0	33.0	41.0
25	37.72707692405247	40.0	36.0	41.0	33.0	41.0
26	37.69159518039693	40.0	36.0	41.0	33.0	41.0
27	37.63915224699567	40.0	36.0	41.0	33.0	41.0
28	37.543898928260795	40.0	36.0	41.0	33.0	41.0
29	37.46074111807098	39.0	36.0	41.0	33.0	41.0
30	37.3409406279096	39.0	35.0	41.0	32.0	41.0
31	37.26437695713149	39.0	35.0	41.0	32.0	41.0
32	37.20034156997482	39.0	35.0	41.0	32.0	41.0
33	37.03960975207642	39.0	35.0	41.0	31.0	41.0
34	36.910518072454686	39.0	35.0	41.0	31.0	41.0
35	36.752490174050614	39.0	35.0	41.0	31.0	41.0
36	36.59432752882789	39.0	35.0	41.0	30.0	41.0
37	36.57111567195332	39.0	35.0	41.0	30.0	41.0
38	36.471582160155315	39.0	35.0	41.0	30.0	41.0
39	36.39339624336438	39.0	35.0	41.0	30.0	41.0
40	36.27698873093861	39.0	35.0	41.0	30.0	41.0
41	36.14570516973343	39.0	35.0	40.0	30.0	41.0
42	36.05053269908974	38.0	35.0	40.0	30.0	41.0
43	35.92781216610531	38.0	35.0	40.0	29.0	41.0
44	35.87984282708337	38.0	35.0	40.0	29.0	41.0
45	35.86463968700692	38.0	35.0	40.0	29.0	41.0
46	35.64588430373941	38.0	35.0	40.0	28.0	41.0
47	35.562311684715695	38.0	35.0	40.0	28.0	41.0
48	35.4559108409364	38.0	34.0	40.0	28.0	41.0
49	35.487134056703574	38.0	34.0	40.0	28.0	41.0
50	35.29992158263573	37.0	34.0	40.0	28.0	41.0
51	34.23575092024151	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	16.0
9	27.0
10	35.0
11	42.0
12	32.0
13	36.0
14	53.0
15	67.0
16	140.0
17	233.0
18	473.0
19	817.0
20	1526.0
21	2392.0
22	3743.0
23	5559.0
24	8459.0
25	12351.0
26	17409.0
27	20568.0
28	23105.0
29	26702.0
30	32328.0
31	39865.0
32	51629.0
33	71256.0
34	114183.0
35	146887.0
36	170547.0
37	250138.0
38	393355.0
39	498260.0
40	201.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.446824678007946	4.4141472534371005	53.67726710201338	17.461760966541572
2	21.87870884013109	4.2719497283398455	52.40314345833258	21.44619797319648
3	20.88464721169201	4.367857758087715	52.010898111325176	22.736596918895096
4	18.673531180413836	4.7685049655523715	51.867062487648205	24.690901366385585
5	17.21514786745986	7.8508252317909495	50.35520318234996	24.578823718399228
6	21.4802809920325	8.020236330067352	53.41961004799101	17.079872629909143
7	83.34407785089921	2.2857287794897374	9.9955718496458	4.374621519965252
8	85.91277494956242	2.713166825016196	7.578848025668476	3.795210199752911
9	80.00267379961721	5.366252421479594	9.596404215091855	5.034669563811338
10	37.884358694974416	27.706640851642167	16.99495571321227	17.414044740171146
11	29.523345018436537	24.560751792132688	25.02967072104872	20.886232468382055
12	25.511218861595463	21.72160990214739	31.53059703937461	21.23657419688254
13	24.01389107595599	20.920051277769733	33.55851023917296	21.507547407101317
14	21.542634421841033	23.15790530522004	32.709922332990566	22.58953793994836
15	19.813647791895956	22.691734154566753	34.974514356613	22.520103696924284
16	22.2399888397929	20.919100123755705	33.19379551668271	23.64711551976868
17	22.25631698370039	21.390079886368802	30.583194799512587	25.77040833041822
18	23.242399486799567	21.181829999186235	31.185116764723595	24.390653749290596
19	24.57993339808226	23.48272440101076	29.13500997126458	22.802332229642396
20	27.167072316239686	23.10786403570421	28.111304042721613	21.613759605334497
21	26.71146954352005	23.18068015966705	27.551866956803867	22.555983340009025
22	24.872783150623693	22.642485513395947	27.434135226623013	25.050596109357347
23	23.663760715014178	23.97864553554727	27.581299889349083	24.77629386008947
24	23.54703298073702	22.772265194421166	29.54876196736696	24.131939857474855
25	23.49567066397948	23.98303141238973	27.605924209934486	24.9153737136963
26	22.75165685745055	24.424842451905953	27.735175472063023	25.088325218580476
27	22.343875994880676	23.782919176216076	28.871223258040686	25.001981570862558
28	21.822749278972413	23.912593173461957	29.23392998872354	25.030727558842088
29	22.717573838614527	23.366208034292274	28.316858993531095	25.599359133562103
30	23.59754982725986	23.74217807928186	28.68194360924902	23.978328484209257
31	23.873278807548783	25.586677080041724	26.103048025879843	24.43699608652965
32	23.886013702958827	25.8353510128205	25.49589471359167	24.782740570629
33	24.18034302841097	25.307936112041716	26.311139387393407	24.20058147215391
34	23.069553665694727	24.356570730454578	27.07829794159703	25.495577662253666
35	23.075841850565247	24.87553092888644	26.363611383833973	25.68501583671433
36	22.91477977085643	27.49014762967136	26.002754119289513	23.592318480182705
37	23.598131088046216	27.355929229914004	25.964707958728372	23.08123172331141
38	22.992721558117097	26.24255061460402	26.81662490395987	23.94810292331902
39	23.558605354574365	25.24711509703356	27.045483128113045	24.148796420279027
40	23.77673667512489	24.565718929761506	28.864353812383815	22.793190582729792
41	21.607418578574304	26.006558735345624	27.864426734191554	24.521595951888518
42	21.718703598215637	26.898899726173326	26.406677523913597	24.97571915169744
43	21.979002746721424	25.106397144847016	27.570625827636096	25.34397428079546
44	22.619393607610924	24.011090455803572	27.74600805944501	25.623507877140494
45	23.31283772572734	23.702493820141004	26.832847364088018	26.151821090043637
46	22.812319346789696	24.57449068344643	27.69480426835648	24.91838570140739
47	21.802457993339807	25.704514494001916	28.04398347528426	24.449044037374012
48	21.596533149302648	24.708392031865774	28.555387283493566	25.139687535338012
49	22.178005303212046	24.271970865095714	28.683158972711393	24.86686485898085
50	21.98735176528901	23.59274121530005	28.801260596119928	25.618646423291015
51	21.240114603490312	23.948789867884706	27.94448219703895	26.866613331586027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	337.0
1	1486.5
2	2636.0
3	9496.5
4	16357.0
5	11001.0
6	5645.0
7	5495.5
8	5346.0
9	5368.0
10	5390.0
11	5358.0
12	5326.0
13	5198.0
14	5070.0
15	4873.0
16	4676.0
17	4730.0
18	4784.0
19	4824.5
20	4865.0
21	5375.5
22	5886.0
23	6635.0
24	7384.0
25	8389.5
26	11998.5
27	14602.0
28	16938.5
29	19275.0
30	22686.0
31	26097.0
32	28815.5
33	31534.0
34	38659.0
35	45784.0
36	49629.0
37	53474.0
38	58143.5
39	62813.0
40	68329.0
41	73845.0
42	81296.5
43	88748.0
44	99321.5
45	109895.0
46	123848.0
47	137801.0
48	153055.5
49	168310.0
50	166737.0
51	165164.0
52	152788.0
53	140412.0
54	130225.5
55	120039.0
56	114228.0
57	108417.0
58	104955.0
59	101493.0
60	96545.5
61	91598.0
62	82809.5
63	74021.0
64	65710.5
65	57400.0
66	48379.5
67	39359.0
68	33773.5
69	28188.0
70	23536.0
71	18884.0
72	15748.5
73	12613.0
74	10457.5
75	6643.0
76	4984.0
77	3995.0
78	3006.0
79	2527.5
80	2049.0
81	1432.5
82	816.0
83	546.5
84	277.0
85	172.5
86	68.0
87	60.0
88	52.0
89	29.5
90	7.0
91	6.5
92	6.0
93	5.0
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1892438.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.07179968231372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.6399492148055	21.69917651870895
2	9.869946272206745	5.738742018015871
3	3.2945306567737824	2.8733380590290656
4	1.5900244170433995	1.8489948536909346
5	0.933810697078598	1.3573778763335371
6	0.6818704181848035	1.189392012405846
7	0.5409796263126393	1.100907592986179
8	0.4359653606339049	1.0139438106221235
9	0.37917875785216015	0.9921068002859887
>10	7.275529508860728	51.91222842180001
>50	0.29102065501596724	5.198862466627492
>100	0.06291335181680835	3.2692031626947013
>500	0.0027919978793737284	0.5486984047031607
>1k	0.00130293234370774	0.7518358614344887
>5k	1.8613319195824856E-4	0.5051921406616083
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9336	0.4933318819427638	No Hit
CTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGCT	2859	0.15107496256152117	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	2802	0.14806297485043105	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	2581	0.1363849172337482	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTGAATCGTATGCCGTCTTCTGC	2013	0.10637072390218333	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.2841889668248046E-5	0.0	0.0	0.16423259308891494	0.0
2	5.2841889668248046E-5	0.0	0.0	0.6056208974877909	0.0
3	5.2841889668248046E-5	0.0	0.0	0.9306513608371846	0.0
4	5.2841889668248046E-5	0.0	0.0	1.3227910240652534	0.0
5	5.2841889668248046E-5	0.0	0.0	1.9811481274419558	0.0
6	5.2841889668248046E-5	0.0	0.0	2.6648693378594173	0.0
7	5.2841889668248046E-5	0.0	0.0	3.2008974666541254	0.0
8	5.2841889668248046E-5	0.0	0.0	4.022430325326378	0.0
9	5.2841889668248046E-5	0.0	0.0	4.530346568817579	0.0
10	5.2841889668248046E-5	0.0	0.0	5.319223139674853	0.0
11	5.2841889668248046E-5	0.0	0.0	6.530359250871099	0.0
12	5.2841889668248046E-5	0.0	0.0	7.565848920810088	0.0
13	5.2841889668248046E-5	0.0	0.0	7.910325199557397	0.0
14	5.2841889668248046E-5	0.0	0.0	8.04232423994868	0.0
15	5.2841889668248046E-5	0.0	0.0	8.232924935982052	0.0
16	5.2841889668248046E-5	0.0	0.0	8.671248410780168	0.0
17	5.2841889668248046E-5	0.0	0.0	9.265772511437627	0.0
18	5.2841889668248046E-5	0.0	0.0	9.913297027432339	0.0
19	5.2841889668248046E-5	0.0	0.0	10.339625393275764	0.0
20	5.2841889668248046E-5	0.0	0.0	10.81567797729701	0.0
21	5.2841889668248046E-5	0.0	0.0	11.482172731682622	0.0
22	5.2841889668248046E-5	0.0	0.0	12.19379445984492	0.0
23	5.2841889668248046E-5	0.0	0.0	12.890673300789775	0.0
24	5.2841889668248046E-5	0.0	0.0	13.445354616637374	0.0
25	5.2841889668248046E-5	0.0	0.0	13.931024424578242	0.0
26	5.2841889668248046E-5	0.0	0.0	14.351170289330483	0.0
27	5.2841889668248046E-5	0.0	0.0	14.779136753753624	0.0
28	5.2841889668248046E-5	0.0	0.0	15.221793263504537	0.0
29	5.2841889668248046E-5	0.0	0.0	15.697528796187775	0.0
30	5.2841889668248046E-5	0.0	0.0	16.24269857189509	0.0
31	5.2841889668248046E-5	0.0	0.0	16.729055324401646	0.0
32	5.2841889668248046E-5	0.0	0.0	17.20933525959635	0.0
33	5.2841889668248046E-5	0.0	0.0	17.692362973053807	0.0
34	1.0568377933649609E-4	0.0	0.0	18.172642908248513	0.0
35	1.0568377933649609E-4	0.0	0.0	18.6968872956472	0.0
36	1.0568377933649609E-4	0.0	0.0	19.18287415492608	0.0
37	1.0568377933649609E-4	0.0	0.0	19.663523983348465	0.0
38	1.5852566900474414E-4	0.0	0.0	20.146657380585257	0.0
39	1.5852566900474414E-4	0.0	0.0	20.63967221119001	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGC	20	7.0344453E-4	45.0	10
CGAACGA	60	0.0	44.999996	22
GCGATCG	65	0.0	44.999996	9
CGTTTTT	5175	0.0	40.391304	1
GTCGTTA	35	6.250433E-6	38.571426	22
TTTGGGA	9940	0.0	38.02817	4
ACGGGTA	30	1.14020084E-4	37.499996	5
TACGTCG	30	1.14020084E-4	37.499996	24
TTTGGGC	5565	0.0	37.439354	4
CGTTTGG	1325	0.0	37.35849	2
AGACACG	230	0.0	37.173916	24
TACGAAT	310	0.0	37.01613	12
TTGGGAC	3940	0.0	36.94797	5
TTGGGAT	5095	0.0	36.830227	5
TGGGCGA	3855	0.0	36.536964	6
TTACGGG	410	0.0	36.219513	3
ACACGAC	230	0.0	36.195656	26
GGGCGAT	6045	0.0	36.17866	7
CCGCTAA	25	0.0021074521	36.0	26
ATACGTA	25	0.0021074521	36.0	37
>>END_MODULE