FastQCFastQC Report
Sat 14 Jan 2017
SRR2933228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748391
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55430.31703434758014654No Hit
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC26660.15248305441974935TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC25310.1447616694435055TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT25060.14333178333679367TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC19470.11135952999071717TruSeq Adapter, Index 23 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG207.0342957E-445.0000049
TACGAAT2150.040.81395312
TTTGGGA92050.038.4003264
CGTTTTT31650.038.1042671
TTACGGG6500.038.076923
TTGGGAC39950.037.959955
TATGCGA301.1401653E-437.49999627
TCAAGCG9200.037.17391617
TTTGGGC52450.036.9780734
TCGTTCA3200.036.56250416
TTGGGAT43200.036.5104145
ACGACCA8650.036.41618728
GGGCGAT48550.036.1946457
CGACCAA8650.036.1560729
CATACGA3300.036.13636418
ACATACG3300.036.13636417
TCTTACG1250.036.0000041
TATCTCG250.002107408436.01
TCGGTAT250.002107408436.05
TTGGGCA24500.036.05
Produced by FastQC (version 0.11.5)