##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1748391
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.299010919182265	33.0	31.0	34.0	31.0	34.0
2	32.4899624855081	34.0	31.0	34.0	31.0	34.0
3	32.55757836776785	34.0	31.0	34.0	31.0	34.0
4	36.0159226397299	37.0	35.0	37.0	35.0	37.0
5	35.2059356288153	37.0	35.0	37.0	35.0	37.0
6	34.887931818454796	37.0	35.0	37.0	32.0	37.0
7	35.68145111705562	37.0	35.0	37.0	35.0	37.0
8	35.970753109573316	37.0	35.0	37.0	35.0	37.0
9	37.85803118410013	39.0	38.0	39.0	35.0	39.0
10	37.358502760538116	39.0	37.0	39.0	34.0	39.0
11	37.28466801762306	39.0	37.0	39.0	34.0	39.0
12	37.12395568268197	39.0	37.0	39.0	34.0	39.0
13	37.09030874672771	39.0	37.0	39.0	33.0	39.0
14	38.28514159590161	40.0	38.0	41.0	33.0	41.0
15	38.36986463554205	40.0	38.0	41.0	34.0	41.0
16	38.3511314116808	40.0	38.0	41.0	34.0	41.0
17	38.30160930821538	40.0	37.0	41.0	34.0	41.0
18	38.25168283295899	40.0	37.0	41.0	34.0	41.0
19	38.246144598090474	40.0	37.0	41.0	34.0	41.0
20	38.17462855848606	40.0	37.0	41.0	34.0	41.0
21	38.055042035791764	40.0	37.0	41.0	33.0	41.0
22	38.07532182446604	40.0	37.0	41.0	34.0	41.0
23	37.9960592338899	40.0	37.0	41.0	34.0	41.0
24	37.93710216993796	40.0	36.0	41.0	33.0	41.0
25	37.817002032154136	40.0	36.0	41.0	33.0	41.0
26	37.790178512701104	40.0	36.0	41.0	33.0	41.0
27	37.76113123437492	40.0	36.0	41.0	33.0	41.0
28	37.67037178754638	40.0	36.0	41.0	33.0	41.0
29	37.602400149623286	40.0	36.0	41.0	33.0	41.0
30	37.4934851529206	40.0	36.0	41.0	33.0	41.0
31	37.428960684423565	40.0	36.0	41.0	33.0	41.0
32	37.374372208504845	39.0	35.0	41.0	33.0	41.0
33	37.24608225505622	39.0	35.0	41.0	32.0	41.0
34	37.11370454320573	39.0	35.0	41.0	32.0	41.0
35	36.98232546381216	39.0	35.0	41.0	31.0	41.0
36	36.85740203421317	39.0	35.0	41.0	31.0	41.0
37	36.835909702120404	39.0	35.0	41.0	31.0	41.0
38	36.726447345016076	39.0	35.0	41.0	31.0	41.0
39	36.675402698824236	39.0	35.0	41.0	31.0	41.0
40	36.555093797668825	39.0	35.0	41.0	31.0	41.0
41	36.461810887839164	39.0	35.0	41.0	30.0	41.0
42	36.38366246451738	39.0	35.0	40.0	30.0	41.0
43	36.26012144880636	38.0	35.0	40.0	30.0	41.0
44	36.1733668269855	38.0	35.0	40.0	30.0	41.0
45	36.14975311586482	38.0	35.0	40.0	30.0	41.0
46	35.940245059600514	38.0	35.0	40.0	30.0	41.0
47	35.86098018120661	38.0	35.0	40.0	29.0	41.0
48	35.76000963171281	38.0	35.0	40.0	29.0	41.0
49	35.793220166427304	38.0	35.0	40.0	29.0	41.0
50	35.60561910922671	37.0	34.0	40.0	29.0	41.0
51	34.55121251482077	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	7.0
9	20.0
10	37.0
11	20.0
12	26.0
13	37.0
14	35.0
15	47.0
16	98.0
17	180.0
18	284.0
19	631.0
20	1171.0
21	1820.0
22	2826.0
23	4165.0
24	6169.0
25	9354.0
26	13055.0
27	15979.0
28	18600.0
29	22257.0
30	27295.0
31	35007.0
32	45475.0
33	64810.0
34	110131.0
35	139634.0
36	154924.0
37	230861.0
38	367668.0
39	475579.0
40	189.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.76602258876876	4.291031010797928	54.898646812984055	17.04429958744926
2	19.796315583871113	4.16171211130691	54.2848253050948	21.75714699972718
3	20.748390949164115	4.293719196678547	52.23694242306212	22.72094743109522
4	18.483737333353922	4.8007568101185605	51.67808573711486	25.03742011941265
5	17.495171274617636	7.779609938509179	50.487219392000995	24.2379993948722
6	21.861357099184335	7.791106222807141	52.74672541782702	17.600811260181505
7	85.06318094751116	2.061037834214429	8.996385819876675	3.8793953983977265
8	88.00920389089168	2.408786135366746	6.266447264942452	3.315562708799119
9	82.67389845863997	4.893356234389219	8.008048542917459	4.42469676405335
10	40.07518913103533	28.937463073191296	15.37476456925253	15.61258322652084
11	30.93575750504321	25.307439811804112	23.30108082231034	20.455721860842342
12	26.411426277074177	21.873539728813522	30.21080524894031	21.50422874517199
13	23.84764048773987	22.579045533865134	31.831209380510423	21.74210459788457
14	22.05301903292799	23.567211224491547	31.505996084399886	22.87377365818058
15	21.238727492877736	22.696353390059777	32.93519584578049	23.129723271281996
16	23.37955297184669	21.771045492684415	31.01062634159064	23.838775193878256
17	23.304398158077912	22.024650092570827	29.145368513107194	25.525583236244064
18	23.761732930448623	21.505773022167237	30.5323008411734	24.200193206210738
19	24.44584763934383	24.20768580940991	27.964168198074685	23.382298353171574
20	27.75408933127658	23.17530804036397	27.088849118989973	21.981753509369472
21	26.891753618040816	24.318301798625136	26.612754240899207	22.177190342434844
22	25.598621818574905	22.796159440308262	27.21816801848099	24.38705072263584
23	24.779068297651953	24.076365069369494	26.62447930697424	24.520087326004308
24	24.20837215474113	23.439493797440047	28.353097219100302	23.99903682871852
25	23.657294049214393	24.68418105561056	26.476285910874626	25.18223898430042
26	23.19052202853938	25.486289966031627	26.438594113101704	24.88459389232729
27	22.847349362928544	24.171652679520772	27.540979105932255	25.44001885161843
28	21.922270247330257	24.46535128583938	28.555797873587775	25.05658059324259
29	23.378466258405588	23.23559203862294	27.544696809809704	25.841244893161768
30	24.234624863660358	23.8591939674821	27.843943374222356	24.062237794635184
31	24.62984538355551	25.599422554794664	26.11160775821884	23.659124303430985
32	24.535186923291185	25.911023335169308	25.46100957966496	24.092780161874547
33	24.761566491705803	25.072766903970567	25.543771387521442	24.62189521680219
34	22.97907047107884	25.143288886753588	27.11664610490445	24.760994537263116
35	23.090944760067973	25.98892353026297	26.429442842018748	24.490688867650316
36	24.13956603528616	27.424815158622984	24.873154803473593	23.562464002617265
37	23.873264046772146	26.623850157087286	25.985949367161005	23.51693642897956
38	23.22621198576291	26.755800047014656	26.32517554711732	23.692812420105113
39	23.851358191617322	25.75636685386736	26.194541152408128	24.197733802107194
40	23.181427952900695	25.84610650592459	27.677790608622445	23.294674932552272
41	21.42472707763881	25.505278853528758	27.67596035440585	25.394033714426577
42	22.226664401727074	25.443965337272957	26.2432144754806	26.08615578551937
43	22.778314461696496	23.735423026085126	27.253171630373295	26.23309088184508
44	22.99880289935146	23.93943917579077	27.254773102812813	25.806984822044953
45	23.44910263207715	24.441157612913816	26.28971437167087	25.82002538333817
46	22.79810408541339	25.171657827110757	27.169094327298644	24.861143760177214
47	21.42564220474711	25.598736209463446	28.486476995134385	24.489144590655066
48	21.829041673172647	24.499554161511927	28.495399484440263	25.17600468087516
49	22.69795486249929	24.456142819312156	28.357615659197517	24.488286658991036
50	22.514128704620422	23.810120276299752	28.343888752573083	25.33186226650675
51	21.609983121624396	23.609135485140335	27.529025258080143	27.251856135155123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	1201.5
2	2145.0
3	6267.5
4	10390.0
5	7061.5
6	3733.0
7	3566.5
8	3400.0
9	3423.5
10	3447.0
11	3476.5
12	3506.0
13	3557.0
14	3608.0
15	3484.0
16	3360.0
17	3366.5
18	3373.0
19	3521.5
20	3670.0
21	4056.0
22	4442.0
23	5212.0
24	5982.0
25	6867.5
26	10203.5
27	12654.0
28	14110.0
29	15566.0
30	19350.0
31	23134.0
32	27094.0
33	31054.0
34	35675.5
35	40297.0
36	44768.0
37	49239.0
38	52641.0
39	56043.0
40	63411.5
41	70780.0
42	77964.0
43	85148.0
44	94311.0
45	103474.0
46	116692.0
47	129910.0
48	141932.5
49	153955.0
50	154585.0
51	155215.0
52	144668.0
53	134121.0
54	125153.0
55	116185.0
56	110210.0
57	104235.0
58	99293.0
59	94351.0
60	89054.5
61	83758.0
62	75712.0
63	67666.0
64	59198.0
65	50730.0
66	43684.0
67	36638.0
68	30800.5
69	24963.0
70	21970.5
71	18978.0
72	15969.5
73	12961.0
74	10646.0
75	6812.5
76	5294.0
77	3849.0
78	2404.0
79	1828.5
80	1253.0
81	892.5
82	532.0
83	407.5
84	283.0
85	214.5
86	146.0
87	78.0
88	10.0
89	10.5
90	11.0
91	6.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1748391.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.33446604958162
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.55740191056894	23.526623751499105
2	9.069799563246688	5.502550538556338
3	2.8929457029045733	2.6326788962412544
4	1.311936978694335	1.5918763095757595
5	0.7850338864874484	1.1906791888712307
6	0.5590869463959159	1.0175762396526704
7	0.4416522203871124	0.9378098999538641
8	0.35354230309402473	0.8579613592237271
9	0.3186376556097458	0.8699132831590902
>10	6.098140476605393	47.32415465496591
>50	0.5543055965288808	10.214005771155989
>100	0.05376974424794065	2.8456870419230724
>500	0.0024355598988617523	0.49136820212702537
>1k	0.0011241045687054242	0.6820959012177376
>5k	1.8735076145090404E-4	0.31501896187718037
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5543	0.31703434758014654	No Hit
TCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2666	0.15248305441974935	TruSeq Adapter, Index 23 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	2531	0.1447616694435055	TruSeq Adapter, Index 23 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGCT	2506	0.14333178333679367	TruSeq Adapter, Index 20 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCGATACTCGTATGCCGTCTTCTGC	1947	0.11135952999071717	TruSeq Adapter, Index 23 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.715863328054194E-4	0.0	0.0	0.1530550088624341	0.0
2	2.2878177707389251E-4	0.0	0.0	0.6036979142537339	0.0
3	2.2878177707389251E-4	0.0	0.0	0.9196455483927795	0.0
4	2.2878177707389251E-4	0.0	0.0	1.276316338850978	0.0
5	2.2878177707389251E-4	0.0	0.0	1.8938555506176822	0.0
6	2.2878177707389251E-4	0.0	0.0	2.469127329069985	0.0
7	2.2878177707389251E-4	0.0	0.0	2.9602646090033637	0.0
8	2.2878177707389251E-4	0.0	0.0	3.717532291117948	0.0
9	2.2878177707389251E-4	0.0	0.0	4.185162243456984	0.0
10	2.8597722134236564E-4	0.0	0.0	4.900620055811315	0.0
11	2.8597722134236564E-4	0.0	0.0	6.015759632713735	0.0
12	2.8597722134236564E-4	0.0	0.0	6.955823954710359	0.0
13	2.8597722134236564E-4	0.0	0.0	7.296194043552043	0.0
14	2.8597722134236564E-4	0.0	0.0	7.411500059197285	0.0
15	2.8597722134236564E-4	0.0	0.0	7.592580835751271	0.0
16	2.8597722134236564E-4	0.0	0.0	7.985513537875681	0.0
17	2.8597722134236564E-4	0.0	0.0	8.560098970996762	0.0
18	2.8597722134236564E-4	0.0	0.0	9.184272854298609	0.0
19	4.5756355414778503E-4	0.0	0.0	9.594192603370756	0.0
20	4.5756355414778503E-4	0.0	0.0	10.033568006241167	0.0
21	4.5756355414778503E-4	0.0	0.0	10.662889479527177	0.0
22	4.5756355414778503E-4	0.0	0.0	11.33179020024697	0.0
23	4.5756355414778503E-4	0.0	0.0	11.988050727783431	0.0
24	4.5756355414778503E-4	0.0	0.0	12.523457281580608	0.0
25	4.5756355414778503E-4	0.0	0.0	12.99200236102794	0.0
26	4.5756355414778503E-4	0.0	0.0	13.400492223993375	0.0
27	4.5756355414778503E-4	0.0	0.0	13.797257020883773	0.0
28	5.147589984162581E-4	0.0	0.0	14.214612177710821	0.0
29	5.147589984162581E-4	0.0	0.0	14.67051706397482	0.0
30	5.147589984162581E-4	0.0	0.0	15.185905212278032	0.0
31	5.147589984162581E-4	0.0	0.0	15.64375474364716	0.0
32	5.147589984162581E-4	0.0	0.0	16.09096592238235	0.0
33	5.147589984162581E-4	0.0	0.0	16.550474121635265	0.0
34	5.147589984162581E-4	0.0	0.0	17.010897447996474	0.0
35	5.147589984162581E-4	0.0	0.0	17.524741319304436	0.0
36	5.719544426847313E-4	0.0	0.0	17.98533623199845	0.0
37	5.719544426847313E-4	0.0	0.0	18.445530776582583	0.0
38	5.719544426847313E-4	0.0	0.0	18.908356311603068	0.0
39	5.719544426847313E-4	0.0	0.0	19.387253766462994	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	20	7.0342957E-4	45.000004	9
TACGAAT	215	0.0	40.813953	12
TTTGGGA	9205	0.0	38.400326	4
CGTTTTT	3165	0.0	38.104267	1
TTACGGG	650	0.0	38.07692	3
TTGGGAC	3995	0.0	37.95995	5
TATGCGA	30	1.1401653E-4	37.499996	27
TCAAGCG	920	0.0	37.173916	17
TTTGGGC	5245	0.0	36.978073	4
TCGTTCA	320	0.0	36.562504	16
TTGGGAT	4320	0.0	36.510414	5
ACGACCA	865	0.0	36.416187	28
GGGCGAT	4855	0.0	36.194645	7
CGACCAA	865	0.0	36.15607	29
CATACGA	330	0.0	36.136364	18
ACATACG	330	0.0	36.136364	17
TCTTACG	125	0.0	36.000004	1
TATCTCG	25	0.0021074084	36.0	1
TCGGTAT	25	0.0021074084	36.0	5
TTGGGCA	2450	0.0	36.0	5
>>END_MODULE