FastQCFastQC Report
Sat 14 Jan 2017
SRR2933227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences949789
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28110.29596047122045No Hit
CTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGCT12250.1289760146727326Illumina PCR Primer Index 9 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC11460.1206583778081237Illumina PCR Primer Index 9 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC10460.11012972354912513Illumina PCR Primer Index 9 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC10020.10549711567516575Illumina PCR Primer Index 9 (95% over 24bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGG253.890292E-545.035
TACGAAT950.042.6315812
CGTTTGG8000.038.531252
TTTGGGA55050.038.0109024
CGTTTTT17000.037.9852941
TCGTTCA950.037.89473716
TTGGGAC20950.037.6968965
TCGTTTG4200.037.51
TTGGGCG13850.037.2021645
TGGGCGA20250.036.4444436
GGGCGAT30900.036.334957
TTTGGGC24350.036.036964
CGAACGA250.002106924536.022
CAAGTCG250.002106924536.027
GATCGAC250.002106924536.09
CGGCATA250.002106924536.016
ATTCCGA250.002106924536.012
ATACCGA250.002106924536.037
GTATGGG3700.035.878383
TATGGGC3200.035.8593754
Produced by FastQC (version 0.11.5)