FastQCFastQC Report
Sat 14 Jan 2017
SRR2933226.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933226.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1319761
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC110860.8400005758618416No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG107950.8179511290301804No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC94780.718160333575549No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT37310.28270270147397897No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC36650.2777017960070043No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36470.27633791269782937No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTT31150.23602758378221514No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC29560.22397994788450332No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC29270.22178258033083262No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT23960.1815480227101725No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT22720.17215238213585643No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTC22290.16889421645282743No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTA19320.14639014185144128No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTTA207.033668E-445.030
CGTACCC253.8911225E-544.99999643
CGATGAA8400.042.0535719
AATACGG1200.041.2499965
CCGATGA8400.040.98214318
CGTTTTT19900.039.7989961
TACGAAT850.039.70588312
CGAATAT850.039.70588314
TCGTTAG403.4587356E-739.3751
TTATGCG750.039.0000041
CGGATCA356.2489926E-638.571436
TTTGGGC38700.038.0813984
TAATACG1300.038.076924
GTTCGCC301.1400137E-437.4999969
TGGGCGA23000.037.3695646
GGGCGAT35400.036.9279677
TTTGGGA59700.036.8969844
GACACGA2200.036.8181825
GCGATAG552.750312E-936.818189
TTGGGAC22050.036.3265345
Produced by FastQC (version 0.11.5)