##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933226.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1319761
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15557968450348	33.0	31.0	34.0	30.0	34.0
2	32.339641798780235	34.0	31.0	34.0	30.0	34.0
3	32.41773699935064	34.0	31.0	34.0	30.0	34.0
4	35.917672972606404	37.0	35.0	37.0	35.0	37.0
5	35.11639986330859	37.0	35.0	37.0	35.0	37.0
6	34.69250265767817	37.0	35.0	37.0	32.0	37.0
7	35.562769319596505	37.0	35.0	37.0	33.0	37.0
8	35.782084028850676	37.0	35.0	37.0	35.0	37.0
9	37.7048473170521	39.0	37.0	39.0	35.0	39.0
10	37.22414058302981	39.0	37.0	39.0	34.0	39.0
11	37.140840652209	39.0	37.0	39.0	34.0	39.0
12	36.95220877113356	39.0	37.0	39.0	33.0	39.0
13	36.88949665886475	39.0	37.0	39.0	33.0	39.0
14	38.02549855617797	40.0	37.0	41.0	33.0	41.0
15	38.064702624187255	40.0	37.0	41.0	33.0	41.0
16	38.12234260597184	40.0	37.0	41.0	33.0	41.0
17	38.01205066674951	40.0	37.0	41.0	33.0	41.0
18	37.99244105561537	40.0	37.0	41.0	33.0	41.0
19	37.98612400275504	40.0	37.0	41.0	33.0	41.0
20	37.827335403910254	40.0	36.0	41.0	33.0	41.0
21	37.78868825491888	40.0	36.0	41.0	33.0	41.0
22	37.83467006526182	40.0	36.0	41.0	33.0	41.0
23	37.72284527274257	40.0	36.0	41.0	33.0	41.0
24	37.69204045277895	40.0	36.0	41.0	33.0	41.0
25	37.59841365216884	39.0	36.0	41.0	33.0	41.0
26	37.542965733947284	39.0	36.0	41.0	33.0	41.0
27	37.455596884587436	39.0	35.0	41.0	33.0	41.0
28	37.36601324027608	39.0	35.0	41.0	32.0	41.0
29	37.28067581933396	39.0	35.0	41.0	32.0	41.0
30	37.19337289100072	39.0	35.0	41.0	32.0	41.0
31	37.13383256513869	39.0	35.0	41.0	32.0	41.0
32	37.06849952377741	39.0	35.0	41.0	32.0	41.0
33	36.96156576834745	39.0	35.0	41.0	31.0	41.0
34	36.78542857380995	39.0	35.0	41.0	31.0	41.0
35	36.67309838675336	39.0	35.0	41.0	31.0	41.0
36	36.51265494282677	39.0	35.0	41.0	30.0	41.0
37	36.523243981296616	39.0	35.0	41.0	30.0	41.0
38	36.409757524279016	39.0	35.0	41.0	30.0	41.0
39	36.40954081837545	39.0	35.0	40.0	30.0	41.0
40	36.32798741590334	39.0	35.0	40.0	30.0	41.0
41	36.20752545347226	39.0	35.0	40.0	30.0	41.0
42	36.171709877773324	38.0	35.0	40.0	30.0	41.0
43	35.99283658177503	38.0	35.0	40.0	30.0	41.0
44	35.96421170196725	38.0	35.0	40.0	30.0	41.0
45	35.98197400893041	38.0	35.0	40.0	30.0	41.0
46	35.76805951986761	38.0	35.0	40.0	29.0	41.0
47	35.64383778578091	38.0	35.0	40.0	29.0	41.0
48	35.57302041809085	38.0	35.0	40.0	29.0	41.0
49	35.67770831233837	38.0	35.0	40.0	29.0	41.0
50	35.47161796719254	37.0	34.0	40.0	28.0	41.0
51	34.43791489519693	36.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	28.0
11	29.0
12	31.0
13	10.0
14	23.0
15	50.0
16	56.0
17	121.0
18	274.0
19	599.0
20	1095.0
21	1989.0
22	3324.0
23	4846.0
24	6315.0
25	8442.0
26	10867.0
27	12957.0
28	14978.0
29	18063.0
30	22520.0
31	29437.0
32	38710.0
33	54206.0
34	89103.0
35	111279.0
36	116132.0
37	170380.0
38	271371.0
39	332392.0
40	117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.76992273601054	4.123473871405505	52.70727048306474	16.399332909519224
2	19.643405131686723	8.841676636906227	50.81473084899463	20.700187382412423
3	19.94755110963273	8.804851787558505	50.45072554803484	20.796871554773933
4	17.93445934529055	4.4002664118730594	53.668580902148186	23.9966933406882
5	20.13235729802593	7.315188128759677	47.93458815649197	24.61786641672242
6	20.574255490198603	10.844918132904366	51.98683701064056	16.593989366256466
7	81.10756417260397	1.8065392142971342	12.777843867185043	4.308052745913844
8	82.87038334971257	5.482356275113449	7.442786989462485	4.204473385711504
9	77.6980074422566	4.555749109119	9.023376202206308	8.722867246418103
10	41.160255531115105	24.840709795182615	16.983378051025905	17.015656622676378
11	31.896835866494012	23.87144339012897	24.055946493342358	20.175774250034664
12	26.283319479814903	20.431729684389826	30.544848650626893	22.740102185168375
13	25.484841573587946	21.181335105371353	33.32504900508501	20.00877431595569
14	22.06407069158734	25.04188258328591	30.676614932552184	22.217431792574565
15	19.442308114878376	23.197609264101608	35.25949016526477	22.100592455755248
16	21.811221880325302	21.668392989336706	31.071534921853278	25.448850208484718
17	21.53117117417472	22.359199885433803	30.618271035437477	25.491357904954004
18	22.23485919041402	21.489421190654976	30.90946012194632	25.366259496984682
19	23.391129151414535	24.727355937931186	28.491143472189286	23.390371438464992
20	26.466761784898935	23.822722447473442	29.595131239671424	20.1153845279562
21	25.61365277501002	25.960154906835402	27.908841070466543	20.51735124768803
22	22.87876365493449	22.256075153001188	29.092691782830375	25.772469409233945
23	23.485009785862744	25.66737462313252	27.26205729673782	23.585558294266917
24	23.7700613974803	22.888538151983578	28.442043673058986	24.899356777477134
25	21.947307126062977	26.752722652055937	26.115561832786387	25.184408389094692
26	21.143525229189226	25.260558540523625	28.306034198616263	25.289882031670885
27	22.75669609876334	25.008694756095988	27.99878159757714	24.235827547563535
28	19.982405905311644	24.99649557760837	30.74958268959304	24.271515827486947
29	22.146964488267194	24.096711450027694	28.54001595743472	25.216308104270396
30	22.857320378462465	24.990661187896897	29.322581891721306	22.829436541919332
31	23.488343722840728	24.672345977794464	26.58049449862513	25.258815800739683
32	24.41737557027371	25.910145852165655	25.435969088342507	24.236509489218125
33	23.616472982608215	22.44853424218476	27.699409211213244	26.235583563993785
34	21.51700194201829	23.920315875374403	27.761996300845382	26.800685881761925
35	22.298203992995703	23.522365034275143	26.824856924852302	27.354574047876852
36	22.475433051893486	25.84028471821792	28.28542440638873	23.39885782349986
37	22.919073983850105	24.594074230106816	28.114560136267098	24.37229164977598
38	21.062980342652953	24.03965566492721	29.071854676717983	25.825509315701854
39	21.260516108598452	23.668527862241724	28.04030426721202	27.030651761947805
40	21.30893396607416	22.45436863189623	29.588917993485186	26.647779408544427
41	19.951794302150162	23.44053203572465	28.19268034136484	28.414993320760352
42	20.419606277197158	23.365063825950305	28.86924223401055	27.346087662841985
43	21.60603321358943	22.956050375787736	29.45934907911357	25.978567331509268
44	21.954808484263438	22.660693868056413	29.022982191472547	26.361515456207602
45	21.83160435866797	22.327224398963146	27.37025870593236	28.470912536436522
46	23.581921272109117	23.75786221899268	27.42572329383881	25.234493215059395
47	19.68704939758032	23.373853296164988	31.940631674977517	24.998465631277178
48	21.050478078985513	22.94044148902718	29.734550422387084	26.274530009600223
49	21.269532892697995	20.954324305688683	31.909035044981632	25.86710775663169
50	21.57057224755088	20.578801767895854	30.253659564118045	27.596966420435216
51	20.382857199144393	21.019942247118987	28.464623518955328	30.1325770347813
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	184.0
1	859.0
2	1534.0
3	4158.5
4	6783.0
5	4589.0
6	2395.0
7	2445.0
8	2495.0
9	2523.0
10	2551.0
11	2577.0
12	2603.0
13	2732.0
14	2861.0
15	2667.0
16	2473.0
17	2627.0
18	2781.0
19	3000.0
20	3219.0
21	3107.5
22	2996.0
23	4019.0
24	5042.0
25	5536.0
26	7383.0
27	8736.0
28	9984.0
29	11232.0
30	13507.0
31	15782.0
32	18223.0
33	20664.0
34	23635.0
35	26606.0
36	31019.5
37	35433.0
38	38060.5
39	40688.0
40	46391.5
41	52095.0
42	58921.5
43	65748.0
44	73654.5
45	81561.0
46	113253.5
47	144946.0
48	141438.5
49	137931.0
50	131262.0
51	124593.0
52	111208.5
53	97824.0
54	89110.0
55	80396.0
56	75079.0
57	69762.0
58	66896.0
59	64030.0
60	58442.0
61	52854.0
62	48517.0
63	44180.0
64	39150.5
65	34121.0
66	28772.0
67	23423.0
68	19289.0
69	15155.0
70	12979.5
71	10804.0
72	8967.5
73	7131.0
74	5901.0
75	3649.5
76	2628.0
77	1965.0
78	1302.0
79	1106.5
80	911.0
81	641.5
82	372.0
83	252.0
84	132.0
85	88.0
86	44.0
87	38.0
88	32.0
89	23.0
90	14.0
91	12.5
92	11.0
93	6.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1319761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.473109757802664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.4567250508025	24.063308992460474
2	10.00493543824266	6.297728623350815
3	3.4833724161825916	3.288976871454509
4	1.6980617844851196	2.137731396745217
5	0.9570698380974898	1.5060982030162362
6	0.6324541865395025	1.1943180017843735
7	0.46508505887675156	1.0246371173319482
8	0.3935071197504833	0.9907914216307006
9	0.29324884055990036	0.8306507650761105
>10	5.085875771832012	40.981204891600214
>50	0.463283160626197	8.842106349137513
>100	0.058529778405686	3.264960099392211
>500	0.004282666690429163	0.9014378008456116
>1k	0.002855111126952775	2.3278032461444327
>5k	2.379259272460646E-4	0.7097381189910871
>10k+	4.758518544921292E-4	1.6385081010386133
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC	11086	0.8400005758618416	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG	10795	0.8179511290301804	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	9478	0.718160333575549	No Hit
CTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGCT	3731	0.28270270147397897	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	3665	0.2777017960070043	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3647	0.27633791269782937	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTCTCCTT	3115	0.23602758378221514	No Hit
CCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	2956	0.22397994788450332	No Hit
TCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	2927	0.22178258033083262	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCT	2396	0.1815480227101725	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCT	2272	0.17215238213585643	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTC	2229	0.16889421645282743	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTA	1932	0.14639014185144128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.577129495416216E-5	0.0	0.0	0.3007362696730696	0.0
2	7.577129495416216E-5	0.0	0.0	1.0986837768353512	0.0
3	7.577129495416216E-5	0.0	0.0	1.5353537496561878	0.0
4	7.577129495416216E-5	0.0	0.0	2.2178258033083265	0.0
5	7.577129495416216E-5	0.0	0.0	3.747496705842952	0.0
6	7.577129495416216E-5	0.0	0.0	4.417466495827654	0.0
7	7.577129495416216E-5	0.0	0.0	5.169648140837621	0.0
8	7.577129495416216E-5	0.0	0.0	6.201956263293127	0.0
9	7.577129495416216E-5	0.0	0.0	6.667040471721774	0.0
10	7.577129495416216E-5	0.0	0.0	8.397808391064746	0.0
11	1.5154258990832432E-4	0.0	0.0	9.956196614386998	0.0
12	1.5154258990832432E-4	0.0	0.0	12.285027364803172	0.0
13	1.5154258990832432E-4	0.0	0.0	12.787769906824039	0.0
14	1.5154258990832432E-4	0.0	0.0	12.992428174495231	0.0
15	1.5154258990832432E-4	0.0	0.0	13.383483827753661	0.0
16	1.5154258990832432E-4	0.0	0.0	14.012234033283299	0.0
17	1.5154258990832432E-4	0.0	0.0	14.817682898646042	0.0
18	1.5154258990832432E-4	0.0	0.0	15.702312767235886	0.0
19	2.2731388486248646E-4	0.0	0.0	16.568530211151867	0.0
20	2.2731388486248646E-4	0.0	0.0	17.19477996394802	0.0
21	0.0010607981293582701	0.0	0.0	18.039023732327294	0.0
22	0.0010607981293582701	0.0	0.0	18.913272933508416	0.0
23	0.0010607981293582701	0.0	0.0	19.78971950224321	0.0
24	0.0010607981293582701	0.0	0.0	20.445747373956344	0.0
25	0.0010607981293582701	0.0	0.0	21.027595147909356	0.0
26	0.0010607981293582701	0.0	0.0	21.546476975755457	0.0
27	0.0010607981293582701	0.0	0.0	22.067025772090552	0.0
28	0.0010607981293582701	0.0	0.0	22.61000287173208	0.0
29	0.0010607981293582701	0.0	0.0	23.19245681604472	0.0
30	0.0010607981293582701	0.0	0.0	23.831360375098217	0.0
31	0.0010607981293582701	0.0	0.0	24.40411559365673	0.0
32	0.0010607981293582701	0.0	0.0	24.95520022185835	0.0
33	0.0010607981293582701	0.0	0.0	25.517423230418235	0.0
34	0.0010607981293582701	0.0	0.0	26.074114934446463	0.0
35	0.0010607981293582701	0.0	0.0	26.661797098110945	0.0
36	0.0010607981293582701	0.0	0.0	27.203713399623112	0.0
37	0.0010607981293582701	0.0	0.0	27.75525265559446	0.0
38	0.0010607981293582701	0.0	0.0	28.302624490343327	0.0
39	0.0010607981293582701	0.0	0.0	28.896292586309187	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACTTA	20	7.033668E-4	45.0	30
CGTACCC	25	3.8911225E-5	44.999996	43
CGATGAA	840	0.0	42.05357	19
AATACGG	120	0.0	41.249996	5
CCGATGA	840	0.0	40.982143	18
CGTTTTT	1990	0.0	39.798996	1
TACGAAT	85	0.0	39.705883	12
CGAATAT	85	0.0	39.705883	14
TCGTTAG	40	3.4587356E-7	39.375	1
TTATGCG	75	0.0	39.000004	1
CGGATCA	35	6.2489926E-6	38.57143	6
TTTGGGC	3870	0.0	38.081398	4
TAATACG	130	0.0	38.07692	4
GTTCGCC	30	1.1400137E-4	37.499996	9
TGGGCGA	2300	0.0	37.369564	6
GGGCGAT	3540	0.0	36.927967	7
TTTGGGA	5970	0.0	36.896984	4
GACACGA	220	0.0	36.81818	25
GCGATAG	55	2.750312E-9	36.81818	9
TTGGGAC	2205	0.0	36.326534	5
>>END_MODULE