FastQCFastQC Report
Sat 14 Jan 2017
SRR2933225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2933225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1527166
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308622.02086741061548No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCTT44590.2919787370855559No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCT44360.2904726794598622No Hit
CGTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCTTC34440.22551575925603373No Hit
CTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCTTCTGCT32650.2137947020821574No Hit
CCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCTTCTGC30640.20063306804892198No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCC26760.17522653071113423No Hit
CGTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCTTCT24470.16023143522053268No Hit
TCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCTTCTGC24310.15918374295918059No Hit
CGCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCTTCTG23040.15086768563469852No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGT21850.14307547444089247No Hit
GCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTCTTCTGC20270.13272951336004074No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCGTCTGAATCGTATGCCGTC19480.12755653281961488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATCG302.1659725E-645.00000437
CACGAGG253.8914106E-545.02
CGTTTTT223300.044.2845951
CGTTATT5300.041.1792451
CGTTTAT3300.040.2272761
GTTTTTT249950.039.49992
CTCGACC403.4592267E-739.37517
CGTTTTC5950.038.5714261
CAACCGA301.1400973E-437.50000441
ATACGAA1200.037.50000419
TGGGCGA24250.037.4845356
TTGGGCG20800.036.8870165
TACGCGG552.750312E-936.818182
CGTTGAT800.036.562525
TCAAGCG9250.036.24324417
TCTTGCG4550.036.09891
TTTGGGC42800.036.0630844
TACGAAA1250.036.00000420
CGAACGA250.002107325236.022
GACACGA8750.036.025
Produced by FastQC (version 0.11.5)