##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933223.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	690775
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05603416452535	33.0	31.0	34.0	30.0	34.0
2	32.240083963664	34.0	31.0	34.0	30.0	34.0
3	32.32783612609026	34.0	31.0	34.0	30.0	34.0
4	35.85072997719952	37.0	35.0	37.0	35.0	37.0
5	35.14176830371684	37.0	35.0	37.0	35.0	37.0
6	34.74196084108429	37.0	35.0	37.0	32.0	37.0
7	35.56631175129384	37.0	35.0	37.0	33.0	37.0
8	35.77165357750353	37.0	35.0	37.0	35.0	37.0
9	37.67055553544932	39.0	37.0	39.0	35.0	39.0
10	37.17576345409142	39.0	37.0	39.0	34.0	39.0
11	37.07303246353733	39.0	37.0	39.0	33.0	39.0
12	36.93158264268394	39.0	37.0	39.0	33.0	39.0
13	36.81690999239984	39.0	35.0	39.0	33.0	39.0
14	37.92483514892693	40.0	37.0	41.0	33.0	41.0
15	37.9980818645724	40.0	37.0	41.0	33.0	41.0
16	38.04450074192031	40.0	37.0	41.0	33.0	41.0
17	37.966912525786256	40.0	37.0	41.0	33.0	41.0
18	37.94376750750968	40.0	37.0	41.0	33.0	41.0
19	37.94238355470305	40.0	37.0	41.0	33.0	41.0
20	37.8053693315479	40.0	36.0	41.0	33.0	41.0
21	37.72723969454598	39.0	36.0	41.0	33.0	41.0
22	37.7639694545981	39.0	36.0	41.0	33.0	41.0
23	37.67121276826752	39.0	36.0	41.0	33.0	41.0
24	37.62544967608845	39.0	36.0	41.0	33.0	41.0
25	37.52218015996526	39.0	35.0	41.0	33.0	41.0
26	37.50089681879049	39.0	35.0	41.0	33.0	41.0
27	37.41925373674496	39.0	35.0	41.0	33.0	41.0
28	37.30549165792045	39.0	35.0	41.0	32.0	41.0
29	37.243006767760846	39.0	35.0	41.0	32.0	41.0
30	37.15153848937787	39.0	35.0	41.0	32.0	41.0
31	37.05810719843654	39.0	35.0	41.0	31.0	41.0
32	36.98711157757591	39.0	35.0	41.0	31.0	41.0
33	36.84790706090985	39.0	35.0	41.0	31.0	41.0
34	36.691818609532774	39.0	35.0	41.0	31.0	41.0
35	36.57046650501248	39.0	35.0	41.0	30.0	41.0
36	36.399716260721654	39.0	35.0	40.0	30.0	41.0
37	36.28578191162101	38.0	35.0	40.0	30.0	41.0
38	36.113436357714164	38.0	35.0	40.0	30.0	41.0
39	36.10968694582172	38.0	35.0	40.0	30.0	41.0
40	36.0445499619992	38.0	35.0	40.0	29.0	41.0
41	35.989589953313306	38.0	35.0	40.0	29.0	41.0
42	35.89544497122797	38.0	35.0	40.0	29.0	41.0
43	35.67201766132243	38.0	35.0	40.0	28.0	41.0
44	35.5404972675618	38.0	34.0	40.0	28.0	41.0
45	35.57490065506134	38.0	34.0	40.0	28.0	41.0
46	35.419559190764	38.0	34.0	40.0	28.0	41.0
47	35.28742933661467	37.0	34.0	40.0	27.0	41.0
48	35.21133654228946	37.0	34.0	40.0	27.0	41.0
49	35.239513589808546	37.0	34.0	40.0	28.0	41.0
50	34.965545944772174	37.0	34.0	40.0	27.0	41.0
51	33.864827186855344	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	13.0
11	20.0
12	11.0
13	13.0
14	13.0
15	28.0
16	33.0
17	80.0
18	154.0
19	311.0
20	590.0
21	1037.0
22	1583.0
23	2256.0
24	3050.0
25	4436.0
26	5939.0
27	7235.0
28	8742.0
29	10277.0
30	12818.0
31	16403.0
32	21590.0
33	29602.0
34	47768.0
35	62611.0
36	64609.0
37	95223.0
38	144163.0
39	150116.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.036444573124392	4.62306829285947	50.7671817885708	17.573305345445334
2	20.14606782237342	7.803409214288299	50.191162100539245	21.85936086279903
3	20.670551192501176	7.882885165212985	51.35362455213347	20.092939090152363
4	18.46245159422388	4.683000977163331	53.31403134160906	23.540516087003727
5	19.077847345372952	7.919944989323585	49.76236835438457	23.239839310918896
6	20.510875465962144	9.684484817777134	52.450363721906555	17.35427599435417
7	80.03242734609678	1.912200065144222	13.147551662987226	4.907820925771778
8	81.5343635771416	4.764214107343202	8.528681553327784	5.172740762187398
9	76.18052187760125	4.95139517208932	10.438565379465095	8.429517570844341
10	39.54934674821759	24.73786688863957	18.3821070536716	17.330679309471243
11	29.962143968730775	24.342948137960985	25.12598168723535	20.568926206072888
12	25.115558611704248	21.22543519959466	31.48289964170678	22.17610654699432
13	22.934530056820236	23.853787412688646	34.11559480293872	19.096087727552387
14	20.21642358220839	26.608085121783503	31.62115015743187	21.554341138576234
15	17.570699576562557	22.757772067605224	38.293510911657194	21.378017444175022
16	19.363179038036986	22.185371503021965	33.4176830371684	25.033766421772647
17	19.67746371828743	22.889218631247513	30.83247077557815	26.6008468748869
18	21.53320545763816	22.0074553943035	31.97075748253773	24.488581665520613
19	22.289747023270966	23.768086569432885	29.33328507835402	24.608881328942132
20	24.733958235315406	24.63363613332851	29.783648800260575	20.848756831095507
21	24.196880315587567	25.95403713220658	28.912598168723534	20.93648438348232
22	21.98747783286888	23.78748507111578	28.726719988418804	25.49831710759654
23	21.5062791791828	26.01744417502081	27.46465925952734	25.011617386269048
24	22.621113966197388	23.266041764684594	29.023777641055336	25.089066628062685
25	21.18852014042199	25.86775722919909	27.88042416126814	25.063298469110784
26	21.98110817559987	24.860772320943866	28.27215808331222	24.88596142014404
27	22.393398718830298	25.094712460642032	28.535775035286452	23.976113785241214
28	19.637219065542325	25.831131699902283	31.03572074843473	23.49592848612066
29	22.22807715971192	24.46426115594803	28.862943795012846	24.444717889327205
30	23.236220187470593	26.79772719047447	28.408237124968334	21.557815497086604
31	23.92573558684087	27.732040099887804	25.47819478122399	22.86402953204734
32	25.10716224530419	28.41504107705114	24.940393036806483	21.53740364083819
33	24.73149723137056	25.653070826245884	25.33082407440918	24.284607867974376
34	24.05283920234519	25.258296840505228	27.396474973761354	23.292388983388225
35	24.951539937027253	24.926061307951215	26.581158843328147	23.54123991169339
36	27.700336578480695	26.13340089030437	25.140023886214756	21.02623864500018
37	23.847272990481706	28.089609496579925	27.85523506206797	20.2078824508704
38	22.10835655604213	30.893851109261337	24.95993630342731	22.037856031269225
39	22.807426441315915	27.859143715392133	27.647931670949294	21.68549817234266
40	24.30559878397452	25.134233288697477	28.40534182620969	22.154826101118307
41	23.83829756432992	23.534725489486448	27.674857949404654	24.952118996778978
42	26.782816401867464	23.60696319351453	26.31870001085737	23.29152039376063
43	26.5569830986935	23.842785277405813	26.164235822083892	23.4359958018168
44	22.86055517353697	24.52173283630705	27.85407694256451	24.763635047591475
45	23.13488473091817	24.67663131989432	26.343889110057546	25.844594839129964
46	25.336325142050597	25.186059136477144	27.395606384133764	22.082009337338494
47	21.853715030219682	25.312149397415943	30.298432919546887	22.535702652817488
48	23.12504071513879	25.31301798704354	28.101769751366216	23.46017154645145
49	23.92457746733741	22.97390611993775	30.57985595888676	22.52166045383808
50	23.899098838261374	23.12200065144222	27.981180558068836	24.99771995222757
51	22.1855162679599	23.3916977308096	26.24660707176722	28.176178929463287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	521.0
2	846.0
3	2232.0
4	3618.0
5	2516.5
6	1415.0
7	1416.0
8	1417.0
9	1527.0
10	1637.0
11	1741.0
12	1845.0
13	1811.5
14	1778.0
15	1704.0
16	1630.0
17	1769.5
18	1909.0
19	1756.5
20	1604.0
21	1986.5
22	2369.0
23	2686.0
24	3003.0
25	3431.0
26	4234.0
27	4609.0
28	5671.0
29	6733.0
30	7823.0
31	8913.0
32	10012.5
33	11112.0
34	12649.5
35	14187.0
36	15887.5
37	17588.0
38	19564.0
39	21540.0
40	25328.0
41	29116.0
42	32429.5
43	35743.0
44	41877.0
45	48011.0
46	62546.0
47	77081.0
48	78847.5
49	80614.0
50	77181.5
51	73749.0
52	62540.5
53	51332.0
54	45527.5
55	39723.0
56	36330.0
57	32937.0
58	29530.0
59	26123.0
60	24339.5
61	22556.0
62	20151.5
63	17747.0
64	16173.0
65	14599.0
66	12210.5
67	9822.0
68	8253.5
69	6685.0
70	5798.0
71	4911.0
72	4080.0
73	3249.0
74	2667.0
75	1728.0
76	1371.0
77	1026.0
78	681.0
79	551.5
80	422.0
81	319.0
82	216.0
83	181.0
84	146.0
85	86.0
86	26.0
87	19.0
88	12.0
89	9.0
90	6.0
91	3.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	690775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.364653285468464
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.475915422064	25.937036648825718
2	10.744883539945391	7.384883948859208
3	3.996912212034164	4.120575071370265
4	1.8454172680081427	2.53668498328465
5	1.0479005782883997	1.8005370025261376
6	0.751014699895528	1.5485015864519982
7	0.5227792766966828	1.257559001195664
8	0.3959297520167154	1.0884790922764744
9	0.32100825216292755	0.9928203558617914
>10	4.501213825616608	38.124217479430804
>50	0.3323154973273855	6.982641785195123
>100	0.057195003898923916	3.303451945791109
>500	0.0037573360225570457	0.9427161789191948
>1k	0.0037573360225570457	3.9798949200117764
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGC	4765	0.6898049292461366	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCG	4249	0.6151062212732077	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTC	3449	0.4992942709275814	No Hit
CCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	3448	0.49914950598964936	No Hit
CTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGCT	3084	0.44645506858238937	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	2993	0.4332814592305744	No Hit
TCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTGC	2281	0.3302088234229669	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2034	0.29445188375375486	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGAAATGGT	1438	0.20817198074626325	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCT	990	0.14331728855271253	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTC	856	0.12391878686982014	No Hit
TCCTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCTG	838	0.12131301798704354	No Hit
GAACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTATGCCGTCTTCT	809	0.11711483478701458	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGAAATGGTCGTA	806	0.11668053997321848	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4476493793203287E-4	0.0	0.0	0.48076435887228114	0.0
2	1.4476493793203287E-4	0.0	0.0	1.8797727190474467	0.0
3	1.4476493793203287E-4	0.0	0.0	2.5438094893416814	0.0
4	1.4476493793203287E-4	0.0	0.0	3.3584017950852303	0.0
5	1.4476493793203287E-4	0.0	0.0	5.046650501248598	0.0
6	1.4476493793203287E-4	0.0	0.0	5.8377908870471575	0.0
7	1.4476493793203287E-4	0.0	0.0	6.746046107632731	0.0
8	1.4476493793203287E-4	0.0	0.0	8.045166660634795	0.0
9	1.4476493793203287E-4	0.0	0.0	8.667800658680468	0.0
10	1.4476493793203287E-4	0.0	0.0	10.402374144982085	0.0
11	1.4476493793203287E-4	0.0	0.0	12.38739097390612	0.0
12	1.4476493793203287E-4	0.0	0.0	14.855198870833485	0.0
13	1.4476493793203287E-4	0.0	0.0	15.484347291086099	0.0
14	1.4476493793203287E-4	0.0	0.0	15.722485613984293	0.0
15	1.4476493793203287E-4	0.0	0.0	16.14881835619413	0.0
16	1.4476493793203287E-4	0.0	0.0	16.898990264557924	0.0
17	1.4476493793203287E-4	0.0	0.0	18.0038362708552	0.0
18	1.4476493793203287E-4	0.0	0.0	19.172089319966705	0.0
19	1.4476493793203287E-4	0.0	0.0	20.161847200608012	0.0
20	1.4476493793203287E-4	0.0	0.0	20.948065578516882	0.0
21	1.4476493793203287E-4	0.0	0.0	22.004125800731064	0.0
22	1.4476493793203287E-4	0.0	0.0	23.06626615033839	0.0
23	1.4476493793203287E-4	0.0	0.0	24.102493576055878	0.0
24	1.4476493793203287E-4	0.0	0.0	24.86728674315081	0.0
25	1.4476493793203287E-4	0.0	0.0	25.534508342079548	0.0
26	1.4476493793203287E-4	0.0	0.0	26.126886468097428	0.0
27	1.4476493793203287E-4	0.0	0.0	26.73750497629474	0.0
28	1.4476493793203287E-4	0.0	0.0	27.37953747602331	0.0
29	1.4476493793203287E-4	0.0	0.0	27.998697115558613	0.0
30	2.8952987586406575E-4	0.0	0.0	28.746118490101697	0.0
31	2.8952987586406575E-4	0.0	0.0	29.38091274293366	0.0
32	2.8952987586406575E-4	0.0	0.0	30.021497593282906	0.0
33	2.8952987586406575E-4	0.0	0.0	30.639354348376823	0.0
34	2.8952987586406575E-4	0.0	0.0	31.231008649705043	0.0
35	2.8952987586406575E-4	0.0	0.0	31.887951938040608	0.0
36	2.8952987586406575E-4	0.0	0.0	32.503637219065546	0.0
37	2.8952987586406575E-4	0.0	0.0	33.10701748036625	0.0
38	2.8952987586406575E-4	0.0	0.0	33.70156708045312	0.0
39	2.8952987586406575E-4	0.0	0.0	34.28337736600196	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTTG	30	2.164239E-6	45.000004	1
CAACGAA	30	2.164239E-6	45.000004	14
AACCCGA	30	2.164239E-6	45.000004	24
GTGCGTT	30	2.164239E-6	45.000004	21
TTCTCGT	30	2.164239E-6	45.000004	1
CTTCACG	35	1.2109922E-7	45.0	1
TAGGTCG	20	7.0313306E-4	45.0	32
CCGTACT	40	6.8084773E-9	45.0	44
CGAGGTA	20	7.0313306E-4	45.0	19
CGATGTA	20	7.0313306E-4	45.0	11
CGGTCGA	35	1.2109922E-7	45.0	41
AATCGCC	20	7.0313306E-4	45.0	42
CATATCG	25	3.889185E-5	44.999996	29
CGTTGAT	75	0.0	42.0	25
TTCACGG	65	0.0	41.53846	2
TTCTTCG	110	0.0	40.909092	1
TGTACGG	55	6.002665E-11	40.909092	2
CGAGACA	235	0.0	40.212765	22
CGTTTTT	1225	0.0	40.040813	1
TACGGGA	90	0.0	40.0	4
>>END_MODULE