##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933221.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2511215
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16273437360003	33.0	31.0	34.0	30.0	34.0
2	32.360203726084784	34.0	31.0	34.0	30.0	34.0
3	32.43410102281167	34.0	31.0	34.0	30.0	34.0
4	35.92647025443859	37.0	35.0	37.0	35.0	37.0
5	35.114033645068226	37.0	35.0	37.0	35.0	37.0
6	34.75475775670343	37.0	35.0	37.0	32.0	37.0
7	35.61322825803446	37.0	35.0	37.0	33.0	37.0
8	35.90656634338358	37.0	35.0	37.0	35.0	37.0
9	37.77680644628198	39.0	38.0	39.0	35.0	39.0
10	37.24864776612118	39.0	37.0	39.0	34.0	39.0
11	37.166117994675886	39.0	37.0	39.0	34.0	39.0
12	36.99574070718756	39.0	37.0	39.0	33.0	39.0
13	36.93226665180003	39.0	37.0	39.0	33.0	39.0
14	37.95555896249425	40.0	37.0	41.0	33.0	41.0
15	38.07243425991005	40.0	37.0	41.0	33.0	41.0
16	38.10687177322531	40.0	37.0	41.0	33.0	41.0
17	38.07290335554702	40.0	37.0	41.0	33.0	41.0
18	38.0169742534988	40.0	37.0	41.0	33.0	41.0
19	38.00245259764696	40.0	37.0	41.0	33.0	41.0
20	37.896396764116176	40.0	36.0	41.0	33.0	41.0
21	37.7604390703305	40.0	36.0	41.0	33.0	41.0
22	37.83562498631141	40.0	36.0	41.0	33.0	41.0
23	37.74272652879184	40.0	36.0	41.0	33.0	41.0
24	37.68327960767995	39.0	36.0	41.0	33.0	41.0
25	37.572928642111485	39.0	36.0	41.0	33.0	41.0
26	37.54965464924349	39.0	35.0	41.0	33.0	41.0
27	37.51773902274397	39.0	35.0	41.0	33.0	41.0
28	37.41849901342577	39.0	35.0	41.0	33.0	41.0
29	37.34642513683615	39.0	35.0	41.0	32.0	41.0
30	37.2505655628849	39.0	35.0	41.0	32.0	41.0
31	37.163370718954766	39.0	35.0	41.0	32.0	41.0
32	37.12765016137607	39.0	35.0	41.0	32.0	41.0
33	36.99872810571775	39.0	35.0	41.0	31.0	41.0
34	36.82176436505835	39.0	35.0	41.0	31.0	41.0
35	36.67807575217574	39.0	35.0	41.0	31.0	41.0
36	36.52002994566375	39.0	35.0	41.0	30.0	41.0
37	36.468038379828094	39.0	35.0	40.0	30.0	41.0
38	36.33809490625056	39.0	35.0	40.0	30.0	41.0
39	36.279800017123186	39.0	35.0	40.0	30.0	41.0
40	36.16198135165647	38.0	35.0	40.0	30.0	41.0
41	36.021624193866316	38.0	35.0	40.0	30.0	41.0
42	35.93919039190193	38.0	35.0	40.0	29.0	41.0
43	35.78785607763572	38.0	35.0	40.0	29.0	41.0
44	35.729579904548196	38.0	35.0	40.0	29.0	41.0
45	35.721675762529294	38.0	35.0	40.0	29.0	41.0
46	35.51941590027138	38.0	34.0	40.0	28.0	41.0
47	35.437207088998754	37.0	34.0	40.0	28.0	41.0
48	35.33901159398936	37.0	34.0	40.0	28.0	41.0
49	35.40375117224133	37.0	34.0	40.0	28.0	41.0
50	35.19109036860643	37.0	34.0	40.0	28.0	41.0
51	34.09962786937797	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	15.0
9	31.0
10	51.0
11	48.0
12	40.0
13	47.0
14	55.0
15	83.0
16	144.0
17	305.0
18	599.0
19	1112.0
20	1909.0
21	3109.0
22	4771.0
23	7154.0
24	10523.0
25	15473.0
26	21733.0
27	26570.0
28	30532.0
29	36934.0
30	46080.0
31	57555.0
32	75689.0
33	106520.0
34	170777.0
35	201918.0
36	233119.0
37	345033.0
38	523100.0
39	589943.0
40	243.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.001403702988394	4.372783692356091	54.28089590098817	16.344916703667348
2	20.519190909579628	4.1990430926862095	51.39946201340785	23.88230398432631
3	20.53942016115705	4.3429973140491756	52.75864471978704	22.35893780500674
4	18.37174435482426	4.700593139177649	49.85307908721475	27.074583418783337
5	17.59188281369775	9.032958149740265	49.3804791704414	23.994679866120585
6	21.80876587627901	8.75162023164086	51.89340617987707	17.546207712203056
7	82.8885220899047	2.028500148334571	9.375103286656062	5.70787447510468
8	85.17590090852435	2.3620836925551973	8.656248071152808	3.8057673277676347
9	79.5091220783565	4.738542896566005	10.84630348257716	4.906031542500344
10	40.5845775849539	26.936602401626303	16.28649876653333	16.19232124688647
11	30.431802932046836	24.214692887705752	25.02780526557861	20.325698914668795
12	25.458194539296713	20.743783387722676	31.072488815175127	22.725533257805488
13	25.03035383270648	20.71965164273071	33.14280139295122	21.10719313161159
14	21.926199070967638	21.977449163054537	33.52321485814635	22.57313690783147
15	20.447392995024323	20.497607731715526	36.469517743403095	22.58548152985706
16	24.48364636241819	20.35241904815	32.26537751646116	22.898557072970654
17	23.605346415977923	20.729766268519423	29.835557688210685	25.82932962729197
18	24.862785544049395	21.25883287571952	31.043777613625277	22.83460396660581
19	24.62847665373136	23.5475257992645	29.408632872932028	22.415364674072112
20	26.139697317832205	24.582801552236667	28.544549152501876	20.732951977429252
21	26.25271034140844	25.656704025740527	27.74700692692581	20.343578705925218
22	25.82733855922332	22.260101186079247	28.256083210716724	23.656477043980704
23	23.909900187757717	24.410574164298954	27.896655602965097	23.782870044978228
24	23.40795192765255	24.786248887490718	28.25946802643342	23.546331158423314
25	23.557282032800856	25.265140579360985	26.269873348160154	24.907704039678006
26	22.388126862893063	23.74352653994182	27.8872577616811	25.981088835484016
27	22.081183809430893	22.30350646997569	30.03104075119016	25.584268969403258
28	21.237687732830523	23.336233655819992	29.439972284332484	25.986106327017
29	21.66485147627742	23.86832668648443	28.305182949289488	26.161638887948662
30	23.259856284706807	23.752964202587194	29.197300908126145	23.789878604579854
31	24.450913203369684	24.45903676108975	27.220050851878476	23.86999918366209
32	23.663445782220958	25.00936797526297	26.269037099571324	25.058149142944753
33	23.950040120021583	23.592523937615855	26.57586068894937	25.881575253413185
34	21.909673205997894	24.847892354895937	28.375109259860267	24.867325179245903
35	22.691963850168147	24.984638909850414	27.495773958024305	24.82762328195714
36	23.979428284714768	26.301531330451596	26.260754256405765	23.458286128427872
37	23.071182674522095	26.824863661614	26.57916586194332	23.524787801920585
38	22.406723438654197	27.01226298823478	27.044836861837794	23.536176711273228
39	23.477360560525483	24.994753535639123	26.735862918945607	24.792022984889783
40	24.59614170829658	22.79386671392135	28.43018220263896	24.17980937514311
41	22.64433750196618	23.067160717023434	26.946159528355796	27.34234225265459
42	22.971231057476164	22.425200550331216	27.611614298258015	26.991954093934613
43	22.62733378065996	22.534151795047418	28.88012376479115	25.95839065950148
44	23.23405204253718	22.788451008774636	28.584211228429268	25.393285720258916
45	22.93471486909723	22.92945844939601	27.103015870803578	27.032810810703182
46	22.78960582825445	22.74397054812113	28.755721831862264	25.71070179176215
47	21.05853142801393	23.790316639554955	30.45852306552804	24.692628866903075
48	20.848513568133352	24.643330021523447	29.468006522738992	25.04014988760421
49	23.10280083545216	23.320902431691433	28.686472484434827	24.88982424842158
50	21.462160746889452	23.155126104296126	29.280368267949974	26.102344880864443
51	21.536387764488506	22.6786635154696	27.394149843800708	28.39079887624118
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	475.0
1	1508.5
2	2542.0
3	9805.0
4	17068.0
5	11098.0
6	5128.0
7	5075.5
8	5023.0
9	5226.0
10	5429.0
11	5618.5
12	5808.0
13	5983.0
14	6158.0
15	5874.0
16	5590.0
17	5380.0
18	5170.0
19	5315.0
20	5460.0
21	6190.0
22	6920.0
23	7764.5
24	8609.0
25	10164.0
26	14046.0
27	16373.0
28	18849.5
29	21326.0
30	30283.0
31	39240.0
32	40702.5
33	42165.0
34	47038.5
35	51912.0
36	58477.0
37	65042.0
38	74243.5
39	83445.0
40	89771.5
41	96098.0
42	106598.5
43	117099.0
44	131867.0
45	146635.0
46	169406.5
47	192178.0
48	217466.0
49	242754.0
50	239608.0
51	236462.0
52	214815.5
53	193169.0
54	177510.0
55	161851.0
56	152125.0
57	142399.0
58	135883.0
59	129367.0
60	120266.5
61	111166.0
62	104290.0
63	97414.0
64	89297.5
65	81181.0
66	69135.5
67	57090.0
68	47184.0
69	37278.0
70	30243.0
71	23208.0
72	18604.0
73	14000.0
74	12073.0
75	8048.5
76	5951.0
77	4302.0
78	2653.0
79	2026.0
80	1399.0
81	1025.0
82	651.0
83	463.5
84	276.0
85	192.0
86	108.0
87	70.0
88	32.0
89	31.5
90	31.0
91	20.0
92	9.0
93	6.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2511215.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.691059606832862
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.62061877229429	19.38321264336393
2	10.773931945481058	5.751353195135914
3	4.175066395816889	3.3431083809970024
4	2.086613337280296	2.2277568384704334
5	1.2726564303798003	1.6984274321143193
6	0.7967961902641194	1.276040076530217
7	0.583944921301219	1.0910276091090714
8	0.47304013316974414	1.0100753912686238
9	0.35351648251769074	0.8492156556189814
>10	5.858029127484044	39.693230463273466
>50	0.8987671617610568	15.039695112180471
>100	0.09784677796200751	4.661403791878657
>500	0.004891906286829586	0.8650681283655756
>1k	0.003821801786585614	2.04831942096844
>5k	3.057441429268491E-4	0.638447290436119
>10k+	1.5287207146342456E-4	0.42361857028873184
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10382	0.4134253737732532	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	9375	0.37332526287076173	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	6272	0.24975957853071123	No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	4789	0.19070449961472832	No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	4310	0.1716300675171182	No Hit
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3749	0.14929028378693182	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3504	0.13953405025057591	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATG	2957	0.11775176557960987	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCCTA	2608	0.10385411046047431	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.964272274576251E-5	0.0	0.0	0.20531893923857575	0.0
2	7.964272274576251E-5	0.0	0.0	0.7204082485967948	0.0
3	7.964272274576251E-5	0.0	0.0	1.122086320765048	0.0
4	7.964272274576251E-5	0.0	0.0	1.6084246072120467	0.0
5	7.964272274576251E-5	0.0	0.0	2.4532746100990956	0.0
6	7.964272274576251E-5	0.0	0.0	3.242812742039212	0.0
7	7.964272274576251E-5	0.0	0.0	3.9089842964461425	0.0
8	7.964272274576251E-5	0.0	0.0	5.04723808992858	0.0
9	7.964272274576251E-5	0.0	0.0	5.737541389327477	0.0
10	7.964272274576251E-5	0.0	0.0	6.771303930567474	0.0
11	7.964272274576251E-5	0.0	0.0	8.349265196329267	0.0
12	7.964272274576251E-5	0.0	0.0	9.713584858325552	0.0
13	7.964272274576251E-5	0.0	0.0	10.31213974112133	0.0
14	1.1946408411864377E-4	0.0	0.0	10.494601218931871	0.0
15	1.1946408411864377E-4	0.0	0.0	10.833719932383328	0.0
16	1.1946408411864377E-4	0.0	0.0	11.326429636649989	0.0
17	1.1946408411864377E-4	0.0	0.0	12.01956025270636	0.0
18	1.1946408411864377E-4	0.0	0.0	12.773338802133628	0.0
19	1.1946408411864377E-4	0.0	0.0	13.290498822283237	0.0
20	2.3892816823728753E-4	0.0	0.0	13.925092037121473	0.0
21	2.3892816823728753E-4	0.0	0.0	14.710847139731166	0.0
22	2.3892816823728753E-4	0.0	0.0	15.561630525462775	0.0
23	2.3892816823728753E-4	0.0	0.0	16.403613390330975	0.0
24	2.787495296101688E-4	0.0	0.0	17.060387103453905	0.0
25	2.787495296101688E-4	0.0	0.0	17.742566845132735	0.0
26	3.1857089098305004E-4	0.0	0.0	18.235754405735868	0.0
27	3.1857089098305004E-4	0.0	0.0	18.73706552405907	0.0
28	3.1857089098305004E-4	0.0	0.0	19.243274669831138	0.0
29	3.583922523559313E-4	0.0	0.0	19.81921101936712	0.0
30	3.583922523559313E-4	0.0	0.0	20.522496082573575	0.0
31	3.982136137288125E-4	0.0	0.0	21.068964624693624	0.0
32	4.380349751016938E-4	0.0	0.0	21.657603988507557	0.0
33	4.7785633647457506E-4	0.0	0.0	22.222151428690893	0.0
34	4.7785633647457506E-4	0.0	0.0	22.866978733401957	0.0
35	4.7785633647457506E-4	0.0	0.0	23.53287153827928	0.0
36	4.7785633647457506E-4	0.0	0.0	24.11294930939804	0.0
37	4.7785633647457506E-4	0.0	0.0	24.67435086203292	0.0
38	4.7785633647457506E-4	0.0	0.0	25.309780325459986	0.0
39	4.7785633647457506E-4	0.0	0.0	26.213207550926544	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATTAG	3045	0.0	41.970444	16
ACTTGAC	3710	0.0	41.30054	6
CTTGACC	3975	0.0	40.867928	7
CATTAGA	3070	0.0	40.749187	17
CGTTTTT	5330	0.0	40.483116	1
CTCAACT	3885	0.0	39.96139	2
CCTGCAT	3320	0.0	39.713856	13
TCAACTT	3855	0.0	39.571983	3
TGCATTA	3210	0.0	39.532707	15
ATTAGAA	3185	0.0	39.34851	18
AACTTGA	3890	0.0	39.21594	5
TTGACCC	3715	0.0	38.822342	8
ACATACG	365	0.0	38.219177	17
TATGGGA	1410	0.0	37.97872	4
ACCCTGC	3505	0.0	37.93866	11
GACTGTA	2365	0.0	37.864693	31
CTGCATT	3385	0.0	37.7548	14
TGGGCGA	4285	0.0	37.75379	6
TCCCGTA	30	1.140307E-4	37.500004	33
TTTGGGA	12555	0.0	37.38351	4
>>END_MODULE