##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933218.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1053561
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05615052189669	33.0	31.0	34.0	30.0	34.0
2	32.23522700631477	34.0	31.0	34.0	30.0	34.0
3	32.30630309967814	34.0	31.0	34.0	30.0	34.0
4	35.83935339292172	37.0	35.0	37.0	35.0	37.0
5	35.07060530904238	37.0	35.0	37.0	33.0	37.0
6	34.72565708108026	37.0	35.0	37.0	32.0	37.0
7	35.545112243144914	37.0	35.0	37.0	33.0	37.0
8	35.807568807121754	37.0	35.0	37.0	35.0	37.0
9	37.66419599814344	39.0	37.0	39.0	35.0	39.0
10	37.12106560512395	39.0	37.0	39.0	33.0	39.0
11	37.062519398497095	39.0	37.0	39.0	33.0	39.0
12	36.9068122301414	39.0	37.0	39.0	33.0	39.0
13	36.83565450885141	39.0	37.0	39.0	33.0	39.0
14	37.86366522678801	40.0	37.0	41.0	33.0	41.0
15	37.96699574111039	40.0	37.0	41.0	33.0	41.0
16	37.986537087078965	40.0	37.0	41.0	33.0	41.0
17	37.89622717621476	40.0	37.0	41.0	33.0	41.0
18	37.88644131663947	40.0	37.0	41.0	33.0	41.0
19	37.87781723127564	40.0	37.0	41.0	33.0	41.0
20	37.77372548907942	39.0	36.0	41.0	33.0	41.0
21	37.66006619455352	39.0	36.0	41.0	33.0	41.0
22	37.74023905592557	39.0	36.0	41.0	33.0	41.0
23	37.64400827289545	39.0	36.0	41.0	33.0	41.0
24	37.57822850314315	39.0	36.0	41.0	33.0	41.0
25	37.47410733692686	39.0	36.0	41.0	33.0	41.0
26	37.445330645306726	39.0	35.0	41.0	33.0	41.0
27	37.415701606266744	39.0	35.0	41.0	33.0	41.0
28	37.31314370976147	39.0	35.0	41.0	32.0	41.0
29	37.23993105287686	39.0	35.0	41.0	32.0	41.0
30	37.1542986120405	39.0	35.0	41.0	32.0	41.0
31	37.08226861092998	39.0	35.0	41.0	32.0	41.0
32	37.02101159780971	39.0	35.0	41.0	31.0	41.0
33	36.94250261731404	39.0	35.0	41.0	31.0	41.0
34	36.80796745513549	39.0	35.0	41.0	31.0	41.0
35	36.6290959896959	39.0	35.0	40.0	31.0	41.0
36	36.49827964398834	39.0	35.0	40.0	30.0	41.0
37	36.522259271176516	39.0	35.0	40.0	30.0	41.0
38	36.38914785190416	39.0	35.0	40.0	30.0	41.0
39	36.35295156141885	39.0	35.0	40.0	30.0	41.0
40	36.237755573716186	38.0	35.0	40.0	30.0	41.0
41	36.125290324907624	38.0	35.0	40.0	30.0	41.0
42	36.02313107641608	38.0	35.0	40.0	30.0	41.0
43	35.90630442850485	38.0	35.0	40.0	29.0	41.0
44	35.84946101839381	38.0	35.0	40.0	29.0	41.0
45	35.82075741224286	38.0	35.0	40.0	29.0	41.0
46	35.56632506328537	38.0	34.0	40.0	28.0	41.0
47	35.44765134624383	38.0	34.0	40.0	28.0	41.0
48	35.385491680121035	37.0	34.0	40.0	28.0	41.0
49	35.48774204815858	37.0	34.0	40.0	28.0	41.0
50	35.29409687716231	37.0	34.0	40.0	28.0	41.0
51	34.17468091548567	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	13.0
10	34.0
11	27.0
12	22.0
13	32.0
14	23.0
15	32.0
16	58.0
17	131.0
18	189.0
19	437.0
20	739.0
21	1353.0
22	2078.0
23	2996.0
24	4248.0
25	6078.0
26	8386.0
27	10550.0
28	12707.0
29	15432.0
30	19528.0
31	24813.0
32	32988.0
33	45879.0
34	72347.0
35	90200.0
36	101619.0
37	147495.0
38	225011.0
39	228041.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.88626667084298	4.268760897565495	54.97754757436921	15.867424857222314
2	21.17456891437705	6.248238118153576	49.5201511825134	23.057041784955974
3	20.891149159849313	6.461609721696228	49.689007091188834	22.958234027265625
4	18.95818087419713	4.772101473004411	50.41074982843898	25.858967824359482
5	18.811155690083442	7.822423191443114	48.73405526590297	24.632365852570473
6	21.373418340276455	8.344557173243885	52.04245411513904	18.239570371340623
7	81.50073892256832	2.1717774291189595	11.494920559891645	4.832563088421079
8	83.56412205842851	3.4983261529232763	8.0362693759545	4.901282412693712
9	77.65463983575702	5.326696793066562	10.102974578595829	6.915688792580591
10	37.512018763033176	27.17175370007052	17.990415362755456	17.325812174140843
11	29.980513705423796	24.129689690487783	24.947013034840886	20.942783569247535
12	23.94014205157556	21.989139689111497	32.39584608769687	21.674872171616073
13	24.00791221391073	23.73341458159518	33.318431490915096	18.940241713578995
14	20.534833768524084	25.946955135962703	32.850969236712444	20.66724185880077
15	18.82947451547656	22.47748350593843	36.02268876695322	22.670353211631788
16	21.43938509493043	22.666271815300682	32.15143688879903	23.742906200969855
17	20.86191497217532	22.357509437042562	30.69219532613679	26.088380264645334
18	23.57784694004429	20.404418918316072	31.58421771496857	24.43351642667107
19	23.505900465184265	23.83336133361049	29.14439695470884	23.516341246496406
20	25.65916923652261	24.89262605582401	29.03059243840651	20.417612269246867
21	24.761451876066026	26.359840578760984	28.86391960218725	20.01478794298574
22	23.07194362737421	22.538609534711327	29.034673834737617	25.354773003176845
23	22.039824936572252	25.911931060470156	27.761088347043977	24.28715565591361
24	21.880365731077745	24.655905068619663	29.384914589663058	24.078814610639533
25	21.86688763156571	26.159852158536623	27.79582767395528	24.17743253594239
26	20.274763397658038	26.23388678965907	27.73688471763856	25.75446509504433
27	21.82854148929203	24.122096394988045	28.750399834466155	25.29896228125377
28	19.0474970125128	25.44930953214859	29.91853343090718	25.58466002443143
29	20.620068510508645	24.9379010802412	28.58363208205315	25.858398327197
30	22.831425992419994	24.268836830520492	29.012463445400883	23.88727373165863
31	23.91470451165144	26.21813070149711	24.87354790088092	24.993616885970532
32	21.828636405485778	27.356080948326678	25.18791033457009	25.627372311617457
33	21.397621969681868	25.11567911112883	25.683752530703014	27.802946388486287
34	20.400812102953697	25.96119256502471	27.245598498805478	26.392396833216115
35	19.173260969227222	27.070478121342763	27.11736672105365	26.638894188376373
36	21.641651503804717	28.48577348630027	25.38894283292567	24.483632176969344
37	21.648960050723215	25.229673459818653	26.05829183122762	27.063074658230512
38	21.23208812778757	27.73811862815727	25.801923191917698	25.22787005213746
39	20.91971893416708	25.19692737297603	26.689674352030874	27.193679340826016
40	21.678004406009713	26.053735853927773	27.151061969833734	25.117197770228778
41	19.0765413677993	26.288273768675946	26.78098373041523	27.85420113310952
42	21.41034073964393	26.208639082122442	27.6601924330912	24.720827745142426
43	21.8148735573925	23.00398363265155	28.754291398409777	26.426851411546178
44	22.190836600823303	23.66697324597247	27.6429176858293	26.49927246737493
45	22.21931145894732	23.32432578654677	25.28548418174173	29.17087857276418
46	23.101367647435698	24.3208509046937	26.580046148253402	25.997735299617204
47	18.97289288422787	26.315514716281257	29.218811250606276	25.492781148884593
48	19.709442547702505	26.139255344493577	28.323561711187107	25.827740396616804
49	21.554328605557725	23.56996889596331	29.632740771535772	25.242961726943197
50	20.959204070765715	23.220677302975336	27.843001022247403	27.977117604011536
51	19.452219662648865	23.045746757900112	26.439570181508238	31.062463397942786
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	160.0
1	649.5
2	1139.0
3	3131.0
4	5123.0
5	3459.0
6	1795.0
7	1871.0
8	1947.0
9	2087.5
10	2228.0
11	2281.5
12	2335.0
13	2502.5
14	2670.0
15	2537.0
16	2404.0
17	2423.0
18	2442.0
19	2689.5
20	2937.0
21	3073.0
22	3209.0
23	3865.5
24	4522.0
25	5291.5
26	6697.0
27	7333.0
28	8706.0
29	10079.0
30	11974.5
31	13870.0
32	16683.5
33	19497.0
34	21187.0
35	22877.0
36	24352.5
37	25828.0
38	28346.0
39	30864.0
40	36551.0
41	42238.0
42	47388.0
43	52538.0
44	60951.5
45	69365.0
46	85708.0
47	102051.0
48	110144.0
49	118237.0
50	115210.5
51	112184.0
52	99526.0
53	86868.0
54	76397.0
55	65926.0
56	60035.0
57	54144.0
58	48893.5
59	43643.0
60	39459.5
61	35276.0
62	31651.5
63	28027.0
64	25249.0
65	22471.0
66	19624.0
67	16777.0
68	14170.0
69	11563.0
70	9766.0
71	7969.0
72	6472.0
73	4975.0
74	4557.5
75	3655.5
76	3171.0
77	2395.0
78	1619.0
79	1067.5
80	516.0
81	436.0
82	356.0
83	235.5
84	115.0
85	70.0
86	25.0
87	30.5
88	36.0
89	20.5
90	5.0
91	5.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1053561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.5261855817957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.26232586681662	25.98521030195294
2	10.692172102938843	7.383198365971305
3	4.003222346872053	4.146479930198887
4	2.0239809724785776	2.795213706792748
5	1.1558893338361667	1.9954224826022844
6	0.7497388753620702	1.5531374129182576
7	0.5392100786678384	1.3031807070562311
8	0.40196991443560853	1.1102790291281888
9	0.3199030691042531	0.9940529458871509
>10	4.430210267297441	37.487958078477554
>50	0.3411285361428862	7.336921276147041
>100	0.07327049378359822	4.510091379478268
>500	0.004025851306216617	0.9817841869191798
>1k	0.002952290957892186	2.417070196470022
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	3497	0.3319219295323194	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTC	2938	0.2788637772278966	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2936	0.27867394484040314	No Hit
CTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGCT	2794	0.2651958453283673	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGC	2786	0.2644365157783935	No Hit
CCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	2748	0.26082970041601766	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCG	2598	0.24659227135400796	No Hit
GCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTGC	2386	0.22647003827970094	No Hit
TCCTGTCTCTTATACACATCTGACGCACGCACATTCGTATGCCGTCTTCTG	1342	0.12737753200811344	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.84748581240194E-4	0.0	0.0	0.2838943354964734	0.0
2	2.84748581240194E-4	0.0	0.0	1.189869404809024	0.0
3	2.84748581240194E-4	0.0	0.0	1.8155569539874767	0.0
4	2.84748581240194E-4	0.0	0.0	2.6623043184020667	0.0
5	2.84748581240194E-4	0.0	0.0	4.067633483016171	0.0
6	2.84748581240194E-4	0.0	0.0	4.94200145981106	0.0
7	2.84748581240194E-4	0.0	0.0	5.797481114050349	0.0
8	2.84748581240194E-4	0.0	0.0	7.0965990578618605	0.0
9	2.84748581240194E-4	0.0	0.0	7.788917775050519	0.0
10	2.84748581240194E-4	0.0	0.0	9.22594894837603	0.0
11	3.796647749869253E-4	0.0	0.0	11.388899171476545	0.0
12	3.796647749869253E-4	0.0	0.0	13.586209056713376	0.0
13	3.796647749869253E-4	0.0	0.0	14.203638897035862	0.0
14	3.796647749869253E-4	0.0	0.0	14.419573237809676	0.0
15	3.796647749869253E-4	0.0	0.0	14.764593602079044	0.0
16	3.796647749869253E-4	0.0	0.0	15.53654700582121	0.0
17	3.796647749869253E-4	0.0	0.0	16.6664293761823	0.0
18	4.745809687336566E-4	0.0	0.0	17.942197936332114	0.0
19	5.69497162480388E-4	0.0	0.0	18.803182729808714	0.0
20	5.69497162480388E-4	0.0	0.0	19.681347354353473	0.0
21	5.69497162480388E-4	0.0	0.0	20.745927383416813	0.0
22	5.69497162480388E-4	0.0	0.0	21.859863833228452	0.0
23	5.69497162480388E-4	0.0	0.0	22.92207095744812	0.0
24	5.69497162480388E-4	0.0	0.0	23.76065552920049	0.0
25	5.69497162480388E-4	0.0	0.0	24.50147642139373	0.0
26	5.69497162480388E-4	0.0	0.0	25.13418776890944	0.0
27	5.69497162480388E-4	0.0	0.0	25.794045147836716	0.0
28	5.69497162480388E-4	0.0	0.0	26.484750289731682	0.0
29	5.69497162480388E-4	0.0	0.0	27.232500064068432	0.0
30	5.69497162480388E-4	0.0	0.0	28.116264744044248	0.0
31	5.69497162480388E-4	0.0	0.0	28.82358021984489	0.0
32	5.69497162480388E-4	0.0	0.0	29.474705308947463	0.0
33	5.69497162480388E-4	0.0	0.0	30.17006134433602	0.0
34	5.69497162480388E-4	0.0	0.0	30.871492016124364	0.0
35	5.69497162480388E-4	0.0	0.0	31.673154188509255	0.0
36	6.644133562271193E-4	0.0	0.0	32.33775737712387	0.0
37	6.644133562271193E-4	0.0	0.0	33.01346576040685	0.0
38	6.644133562271193E-4	0.0	0.0	33.698286098289515	0.0
39	6.644133562271193E-4	0.0	0.0	34.52548072679228	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAT	30	2.1653286E-6	45.000004	45
GATAACG	30	2.1653286E-6	45.000004	17
GTTTACG	35	1.2117926E-7	45.0	1
TAAGTCG	20	7.0330186E-4	45.0	16
TCGGATA	20	7.0330186E-4	45.0	13
CATAGCG	25	3.890584E-5	45.0	10
AGCGAAC	20	7.0330186E-4	45.0	31
ACACGAC	1005	0.0	40.074627	26
CACGACC	1010	0.0	39.87624	27
TGGTCAA	1120	0.0	39.57589	14
AACACGT	900	0.0	39.5	41
GTCAAGC	1125	0.0	39.4	16
TTCGGGT	160	0.0	39.375	4
AGACACG	1030	0.0	39.32039	24
CGACCAA	1040	0.0	39.158657	29
ATGGTCA	1135	0.0	39.052864	13
GACACGA	1035	0.0	38.913044	25
CGAGACA	1090	0.0	38.807343	22
TCAAGCG	1145	0.0	38.711792	17
GCGAGAC	1105	0.0	38.687786	21
>>END_MODULE