##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2933217.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2561830
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13096653564054	33.0	31.0	34.0	30.0	34.0
2	32.40614482615943	34.0	31.0	34.0	30.0	34.0
3	32.31113696068826	33.0	31.0	34.0	30.0	34.0
4	35.956743812040614	37.0	35.0	37.0	35.0	37.0
5	35.203767619240935	37.0	35.0	37.0	35.0	37.0
6	34.9990362358158	37.0	35.0	37.0	33.0	37.0
7	35.773692243435356	37.0	35.0	37.0	35.0	37.0
8	36.02595878727316	37.0	35.0	37.0	35.0	37.0
9	37.91039569370333	39.0	38.0	39.0	35.0	39.0
10	37.370814612991495	39.0	37.0	39.0	34.0	39.0
11	37.320630955215606	39.0	37.0	39.0	34.0	39.0
12	37.326556406943475	39.0	37.0	39.0	34.0	39.0
13	37.40023147515643	39.0	37.0	39.0	34.0	39.0
14	38.6946951983543	40.0	38.0	41.0	35.0	41.0
15	38.736030493826675	40.0	38.0	41.0	35.0	41.0
16	38.68742890824137	40.0	38.0	41.0	34.0	41.0
17	38.616631860818245	40.0	38.0	41.0	34.0	41.0
18	38.303979967445144	39.0	38.0	41.0	34.0	41.0
19	38.00858370774017	39.0	37.0	41.0	34.0	41.0
20	37.63453117498039	39.0	35.0	41.0	34.0	41.0
21	37.50795056658717	39.0	35.0	41.0	33.0	41.0
22	37.548019970099496	39.0	35.0	41.0	33.0	41.0
23	37.467292521361685	39.0	35.0	41.0	33.0	41.0
24	37.387991787120924	39.0	35.0	41.0	33.0	41.0
25	37.29218761588396	39.0	35.0	41.0	33.0	41.0
26	37.266708563800094	39.0	35.0	41.0	33.0	41.0
27	37.22765327910127	39.0	35.0	41.0	33.0	41.0
28	37.11626376457454	39.0	35.0	41.0	33.0	41.0
29	36.97207894356768	39.0	35.0	41.0	33.0	41.0
30	36.71121854299466	39.0	35.0	41.0	32.0	41.0
31	36.33952018674151	39.0	35.0	41.0	31.0	41.0
32	35.750450264069045	39.0	35.0	41.0	27.0	41.0
33	34.91560212816619	39.0	35.0	41.0	21.0	41.0
34	34.16637130488752	38.0	34.0	41.0	15.0	41.0
35	33.697863246195105	38.0	33.0	40.0	10.0	41.0
36	33.41175878180832	38.0	33.0	40.0	10.0	41.0
37	33.32524211208394	38.0	33.0	40.0	10.0	41.0
38	33.21569034635397	38.0	33.0	40.0	10.0	41.0
39	33.13325044987372	38.0	33.0	40.0	10.0	41.0
40	33.06547350917118	38.0	33.0	40.0	10.0	41.0
41	32.96964708821429	38.0	32.0	40.0	9.0	41.0
42	32.87437730060152	38.0	32.0	40.0	9.0	41.0
43	32.76010000663588	38.0	32.0	40.0	8.0	41.0
44	32.696746466393165	38.0	31.0	40.0	8.0	41.0
45	32.733006483646456	38.0	31.0	40.0	8.0	41.0
46	32.563540906305256	38.0	31.0	40.0	8.0	41.0
47	32.51827521732511	38.0	31.0	40.0	8.0	41.0
48	32.405976196703136	38.0	31.0	40.0	8.0	41.0
49	32.43447028101006	38.0	31.0	40.0	8.0	41.0
50	32.26257597108317	38.0	31.0	40.0	8.0	41.0
51	31.367302670356736	36.0	28.0	40.0	7.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	5.0
10	15.0
11	13.0
12	9.0
13	19.0
14	18.0
15	58.0
16	153.0
17	408.0
18	1068.0
19	2247.0
20	4174.0
21	6970.0
22	11814.0
23	19987.0
24	37770.0
25	73629.0
26	104219.0
27	94029.0
28	68254.0
29	52115.0
30	48143.0
31	51569.0
32	60692.0
33	78215.0
34	115038.0
35	151065.0
36	183609.0
37	275326.0
38	482500.0
39	638484.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	18.837276478142577	3.761490809304286	48.1496039940199	29.251628718533233
2	34.12240468727433	3.3077526611836072	43.83596881916443	18.733873832377636
3	15.045377718271705	3.249005593657659	61.89009419048103	19.815522497589612
4	13.269615860537193	3.499802875288368	62.32123130730767	20.90934995686677
5	12.926111412544937	6.613124211989085	62.00754929093656	18.45321508452942
6	15.881615876151034	5.985096591108699	64.19348668725091	13.939800845489358
7	69.1577505142808	0.9786363654106635	25.96764812653455	3.8959649937739824
8	72.1755151590855	1.1475780984686728	23.386563511240013	3.2903432312058176
9	66.93117810315282	3.64442605481238	25.29750217617875	4.126893665856048
10	28.946768520940108	17.174793019052785	37.22881690041884	16.64962155958826
11	21.47066745256321	23.01987251300828	37.48999738468204	18.01946264974647
12	15.38903830464941	20.30099577255321	45.09518586323058	19.214780059566795
13	16.751033440938702	20.280385505673678	46.97962003723901	15.988961016148611
14	17.15258233372238	21.930729205294654	45.42299840348501	15.493690057497961
15	15.694679194169792	22.835160803019715	45.558877833423765	15.911282169386729
16	15.90464628800506	21.661585663373447	44.708274944082945	17.725493104538554
17	16.5487561625869	22.821498694292753	42.80643134009673	17.82331380302362
18	17.543708989277196	21.57961301101166	43.07104686883985	17.80563113087129
19	17.692821147382926	24.70897756681747	40.75524137042661	16.842959915372997
20	18.522423423880586	23.244399511286854	40.99561641482846	17.2375606500041
21	18.33466701537573	24.053547659290427	41.30761994355597	16.304165381777867
22	17.85333140762658	22.141906371617164	41.0488205696709	18.955941651085357
23	16.413579355382677	23.251894153788502	40.47091337052029	19.86361312030853
24	16.486691154370117	22.81478474371836	41.739420648520785	18.959103453390743
25	16.670934449202328	24.394202581748203	40.26262476432863	18.672238204720845
26	16.44949118403641	24.941116311386782	40.49975993723237	18.109632567344438
27	17.005695147609327	23.955102407263556	40.18478977918129	18.854412665945826
28	17.53172536819383	23.243735923148687	40.34127947599958	18.883259232657903
29	17.42324822490173	22.693387149030187	39.831019232345625	20.052345393722458
30	17.516189598841454	23.523692048262376	39.23777143682446	19.722346916071714
31	16.79377632395592	24.377105428541316	38.2537873317121	20.575330915790666
32	16.88226775391029	25.361440845020944	37.37020020844474	20.386091192624022
33	18.620127018576564	24.501274479571244	35.67383471971208	21.20476378214011
34	17.9592713021551	25.555091477576575	34.234746255606346	22.25089096466198
35	18.506302135582768	26.06597627477233	32.3741231853792	23.053598404265703
36	18.96530995421242	27.20840180652112	31.219245617390694	22.60704262187577
37	19.100955176573	28.327640788030433	31.508452941842357	21.062951093554215
38	19.7334717760351	27.8884235097567	32.118993063552224	20.259111650655974
39	20.680724325970107	25.881967187518296	32.20666476698298	21.23064371952862
40	20.400455924085517	25.823220119992353	32.00181120527123	21.774512750650903
41	19.36592201668339	26.353114765616763	31.644137198799292	22.636826018900553
42	20.140524546905922	27.74270736153453	30.941787706444224	21.174980385115326
43	20.147394635865766	26.66781168149331	32.31963088885679	20.86516279378413
44	20.10875038546664	26.43274534219679	31.303833587708784	22.154670684627785
45	20.320669209120044	25.573476772463433	31.032191831620366	23.073662186796156
46	20.288738909295308	26.019525105100648	30.47204537381481	23.21969061178923
47	19.703376102239414	26.29097168820726	31.242744444401072	22.762907765152253
48	19.51694687001089	25.829973105163106	31.795083982934074	22.85799604189193
49	20.39100955176573	24.70897756681747	31.92218062869121	22.97783225272559
50	19.607350995187034	24.034498776265405	32.229539040451556	24.12861118809601
51	18.785282395787387	23.954829165089016	31.949700019126954	25.310188419996642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	174.0
1	654.5
2	1135.0
3	35578.5
4	70022.0
5	51306.5
6	32591.0
7	33788.0
8	34985.0
9	36193.0
10	37401.0
11	38165.5
12	38930.0
13	39211.0
14	39492.0
15	39133.0
16	38774.0
17	37704.5
18	36635.0
19	35459.5
20	34284.0
21	32973.5
22	31663.0
23	30749.0
24	29835.0
25	29656.0
26	30858.0
27	32239.0
28	33790.0
29	35341.0
30	39885.0
31	44429.0
32	50226.5
33	56024.0
34	64161.5
35	72299.0
36	81137.0
37	89975.0
38	98452.5
39	106930.0
40	117722.0
41	128514.0
42	139516.5
43	150519.0
44	170274.5
45	190030.0
46	215279.0
47	240528.0
48	250786.5
49	261045.0
50	243383.0
51	225721.0
52	190935.0
53	156149.0
54	132137.0
55	108125.0
56	91520.0
57	74915.0
58	63165.0
59	51415.0
60	43126.0
61	34837.0
62	27955.5
63	21074.0
64	16902.5
65	12731.0
66	9737.0
67	6743.0
68	5068.0
69	3393.0
70	2471.5
71	1550.0
72	1214.0
73	878.0
74	724.5
75	387.5
76	204.0
77	165.0
78	126.0
79	98.0
80	70.0
81	49.0
82	28.0
83	21.5
84	15.0
85	12.0
86	9.0
87	5.0
88	1.0
89	2.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2561830.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.80673983520964
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.65970884016974	32.46699243159106
2	8.186882552839348	6.845337378959432
3	3.1843412677388336	3.993807807806371
4	1.9135095683539605	3.199903867854333
5	1.348647325708559	2.819127393767448
6	1.0886668094092533	2.7308166040920243
7	0.8537276507711062	2.498409885013864
8	0.7381238712566112	2.4686842121426333
9	0.6346670482598549	2.388002415172217
>10	4.2488716141271174	29.522208044501586
>50	0.09433882435290795	2.7196821227503576
>100	0.043308376476671635	3.3095494277778794
>500	0.0033130685229554265	0.9596271116985735
>1k	0.0014198865098378186	1.6150497775382844
>5k	3.786364026234183E-4	0.8898187804255796
>10k+	9.465910065585457E-5	1.572982738908425
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39748	1.5515471362268378	No Hit
CTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGCT	6095	0.2379158648309997	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCT	5677	0.2215994035513676	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTT	5470	0.21351924210427703	No Hit
CGCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTG	5243	0.20465838872993133	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCC	4717	0.1841261910431215	No Hit
CCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	4459	0.1740552651815304	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTC	3825	0.14930733108754288	No Hit
TCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	3645	0.14228110374224676	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGT	3399	0.13267859303700869	No Hit
GCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCTGC	3185	0.12432518941537885	No Hit
CGTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTCTTCT	3164	0.12350546289176095	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCGTC	2896	0.11304419106654226	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCACAGTCAGTCGTATGCCG	2635	0.10285616141586287	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.806919272551262E-5	0.0	0.0	0.25505205263424974	0.0
2	7.806919272551262E-5	0.0	0.0	0.7416963654887326	0.0
3	7.806919272551262E-5	0.0	0.0	1.2532057162262913	0.0
4	7.806919272551262E-5	0.0	0.0	1.8131960356463934	0.0
5	7.806919272551262E-5	0.0	0.0	2.835395010597893	0.0
6	7.806919272551262E-5	0.0	0.0	4.438702021601745	0.0
7	7.806919272551262E-5	0.0	0.0	5.719466162860143	0.0
8	7.806919272551262E-5	0.0	0.0	7.232017737320588	0.0
9	7.806919272551262E-5	0.0	0.0	8.217407087902007	0.0
10	7.806919272551262E-5	0.0	0.0	9.381028405475773	0.0
11	7.806919272551262E-5	0.0	0.0	10.934175960153484	0.0
12	7.806919272551262E-5	0.0	0.0	12.275287587388702	0.0
13	7.806919272551262E-5	0.0	0.0	12.954216321926124	0.0
14	7.806919272551262E-5	0.0	0.0	13.25568050963569	0.0
15	1.1710378908826894E-4	0.0	0.0	13.514636021906215	0.0
16	1.1710378908826894E-4	0.0	0.0	13.958186140376215	0.0
17	1.1710378908826894E-4	0.0	0.0	14.604520986950735	0.0
18	1.1710378908826894E-4	0.0	0.0	15.344304657217693	0.0
19	1.1710378908826894E-4	0.0	0.0	15.830051174355832	0.0
20	1.1710378908826894E-4	0.0	0.0	16.31946694355207	0.0
21	1.1710378908826894E-4	0.0	0.0	16.93449604384366	0.0
22	1.1710378908826894E-4	0.0	0.0	17.580830890418177	0.0
23	1.1710378908826894E-4	0.0	0.0	18.24988387207582	0.0
24	1.1710378908826894E-4	0.0	0.0	18.791645034994517	0.0
25	1.1710378908826894E-4	0.0	0.0	19.275556926103604	0.0
26	1.1710378908826894E-4	0.0	0.0	19.70981681063927	0.0
27	1.1710378908826894E-4	0.0	0.0	20.157270388745545	0.0
28	1.1710378908826894E-4	0.0	0.0	20.620376839993288	0.0
29	1.1710378908826894E-4	0.0	0.0	21.132901090236277	0.0
30	1.1710378908826894E-4	0.0	0.0	21.688090154303758	0.0
31	1.5613838545102524E-4	0.0	0.0	22.190894790052422	0.0
32	1.5613838545102524E-4	0.0	0.0	22.68437015727039	0.0
33	1.5613838545102524E-4	0.0	0.0	23.157469465186995	0.0
34	1.5613838545102524E-4	0.0	0.0	23.61963908612203	0.0
35	1.5613838545102524E-4	0.0	0.0	24.08446305960973	0.0
36	1.9517298181378156E-4	0.0	0.0	24.546203299984775	0.0
37	2.3420757817653787E-4	0.0	0.0	25.02523586654852	0.0
38	2.3420757817653787E-4	0.0	0.0	25.49267515799253	0.0
39	2.3420757817653787E-4	0.0	0.0	25.96058286459289	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	20	7.034912E-4	45.0	9
CGTTTTT	46840	0.0	44.20741	1
CGTTATT	1320	0.0	42.954544	1
TACGGGA	210	0.0	42.857143	4
CTGCGCG	95	0.0	40.26316	1
GTCCGCG	45	1.9299478E-8	40.0	1
GTTTTTT	51975	0.0	39.896103	2
TTGTACG	85	0.0	39.705883	1
TAGTCCG	40	3.4604454E-7	39.375	1
ACGGGTA	155	0.0	39.193546	5
GCGATCG	150	0.0	39.000004	9
TACGGGT	280	0.0	38.571426	4
TTACGGG	650	0.0	37.730766	3
AGGGCGA	600	0.0	37.500004	6
CGACGGG	120	0.0	37.499996	3
TAGGGCG	395	0.0	37.025314	5
CGTTTCT	705	0.0	37.021275	1
CGTTTAT	630	0.0	36.785713	1
TTGCGCG	270	0.0	36.666664	1
TGGGCGA	3940	0.0	36.54822	6
>>END_MODULE